LOCUS       Exported               11520 bp DNA     circular SYN 20-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11520)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11520)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11520)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(31..11520,1..30)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..30
                     /label=T7 promoter
     CDS             75..1679
                     /codon_start=1
                     /label=nsp1
                     /translation="MEKVHVDIEEDSPFLRALQRSFPQFEVEAKQVTDNDHANARAFSH
                     LASKLIETEVDPSDTILDIGSAPARRMYSKHKYHCICPMRCAEDPDRLYKYATKLKKNC
                     KEITDKELDKKMKELAAVMSDPDLETETMCLHDDESCRYEGQVAVYQDVYAVDGPTSLY
                     HQANKGVRVAYWIGFDTTPFMFKNLAGAYPSYSTNWADETVLTARNIGLCSSDVMERSR
                     RGMSILRKKYLKPSNNVLFSVGSTIYHEKRDLLRSWHLPSVFHLRGKQNYTCRCETIVS
                     CDGYVVKRIAISPGLYGKPSGYAATMHREGFLCCKVTDTLNGERVSFPVCTYVPATLCD
                     QMTGILATDVSADDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFARWAKEYK
                     EDQEDERPLGLRDRQLVMGCCWAFRRHKITSIYKRPDTQTIIKVNSDFHSFVLPRIGSN
                     TLEIGLRTRIRKMLEEHKEPSPLITAEDVQEAKCAADEAKEVREAEELRAALPPLAADV
                     EEPTLEADVDLMLQEAGA"
     CDS             1680..4061
                     /codon_start=1
                     /label=nsp2
                     /translation="GSVETPRGLIKVTSYDGEDKIGSYAVLSPQAVLKSEKLSCIHPLA
                     EQVIVITHSGRKGRYAVEPYHGKVVVPEGHAIPVQDFQALSESATIVYNEREFVNRYLH
                     HIATHGGALNTDEEYYKTVKPSEHDGEYLYDIDRKQCVKKELVTGLGLTGELVDPPFHE
                     FAYESLRTRPAAPYQVPTIGVYGVPGSGKSGIIKSAVTKKDLVVSAKKENCAEIIRDVK
                     KMKGLDVNARTVDSVLLNGCKHPVETLYIDEAFACHAGTLRALIAIIRPKKAVLCGDPK
                     QCGFFNMMCLKVHFNHEICTQVFHKSISRRCTKSVTSVVSTLFYDKKMRTTNPKETKIV
                     IDTTGSTKPKQDDLILTCFRGWVKQLQIDYKGNEIMTAAASQGLTRKGVYAVRYKVNEN
                     PLYAPTSEHVNVLLTRTEDRIVWKTLAGDPWIKTLTAKYPGNFTATIEEWQAEHDAIMR
                     HILERPDPTDVFQNKANVCWAKALVPVLKTAGIDMTTEQWNTVDYFETDKAHSAEIVLN
                     QLCVRFFGLDLDSGLFSAPTVPLSIRNNHWDNSPSPNMYGLNKEVVRQLSRRYPQLPRA
                     VATGRVYDMNTGTLRNYDPRINLVPVNRRLPHALVLHHNEHPQSDFSSFVSKLKGRTVL
                     VVGEKLSVPGKMVDWLSDRPEATFRARLDLGIPGDVPKYDIIFVNVRTPYKYHHYQQCE
                     DHAIKLSMLTKKACLHLNPGGTCVSIGYGYADRASESIIGAIARQFKFSRVCKPKSSLE
                     ETEVLFVFIGYDRKARTHNSYKLSSTLTNIYTGSRLHEAGC"
     CDS             4062..5732
                     /codon_start=1
                     /label=nsp3
                     /translation="APSYHVVRGDIATATEGVIINAANSKGQPGGGVCGALYKKFPESF
                     DLQPIEVGKARLVKGAAKHIIHAVGPNFNKVSEVEGDKQLAEAYESIAKIVNDNNYKSV
                     AIPLLSTGIFSGNKDRLTQSLNHLLTALDTTDADVAIYCRDKKWEMTLKEAVARREAVE
                     EICISDDSSVTEPDAELVRVHPKSSLAGRKGYSTSDGKTFSYLEGTKFHQAAKDIAEIN
                     AMWPVATEANEQVCMYILGESMSSIRSKCPVEESEASTPPSTLPCLCIHAMTPERVQRL
                     KASRPEQITVCSSFPLPKYRITGVQKIQCSQPILFSPKVPAYIHPRKYLVETPPVDETP
                     EPSAENQSTEGTPEQPPLITEDETRTRTPEPIIIEEEEEDSISLLSDGPTHQVLQVEAD
                     IHGPPSVSSSSWSIPHASDFDVDSLSILDTLEGASVTSGATSAETNSYFAKSMEFLARP
                     VPAPRTVFRNPPHPAPRTRTPSLAPSRACSRTSLVSTPPGVNRVITREELEALTPSRTP
                     SRSVSRTSLVSNPPGVNRVITREEFEAFVAQQQ*RFDAGA"
     CDS             5733..7556
                     /codon_start=1
                     /label=nsp4
                     /translation="YIFSSDTGQGHLQQKSVRQTVLSEVVLERTELEISYAPRLDQEKE
                     ELLRKKLQLNPTPANRSRYQSRKVENMKAITARRILQGLGHYLKAEGKVECYRTLHPVP
                     LYSSSVNRAFSSPKVAVEACNAMLKENFPTVASYCIIPEYDAYLDMVDGASCCLDTASF
                     CPAKLRSFPKKHSYLEPTIRSAVPSAIQNTLQNVLAAATKRNCNVTQMRELPVLDSAAF
                     NVECFKKYACNNEYWETFKENPIRLTEENVVNYITKLKGPKAAALFAKTHNLNMLQDIP
                     MDRFVMDLKRDVKVTPGTKHTEERPKVQVIQAADPLATAYLCGIHRELVRRLNAVLLPN
                     IHTLFDMSAEDFDAIIAEHFQPGDCVLETDIASFDKSEDDAMALTALMILEDLGVDAEL
                     LTLIEAAFGEISSIHLPTKTKFKFGAMMKSGMFLTLFVNTVINIVIASRVLRERLTGSP
                     CAAFIGDDNIVKGVKSDKLMADRCATWLNMEVKIIDAVVGEKAPYFCGGFILCDSVTGT
                     ACRVADPLKRLFKLGKPLAADDEHDDDRRRALHEESTRWNRVGILSELCKAVESRYETV
                     GTSIIVMAMTTLASSVKSFSYLRGAPITLYG"
     promoter        7543..7566
                     /label=26S subgenomic promoter
     CDS             7636..8352
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    8427..8989
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             8992..9588
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        9788..9892
                     /label=AmpR promoter
     CDS             9893..10750
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10924..11512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caacgcgagc tctaatacga ctcactatag atgggcggcg catgagagaa gcccagacca
       61 attacctacc caaaatggag aaagttcacg ttgacatcga ggaagacagc ccattcctca
      121 gagctttgca gcggagcttc ccgcagtttg aggtagaagc caagcaggtc actgataatg
      181 accatgctaa tgccagagcg ttttcgcatc tggcttcaaa actgatcgaa acggaggtgg
      241 acccatccga cacgatcctt gacattggaa gtgcgcccgc ccgcagaatg tattctaagc
      301 acaagtatca ttgtatctgt ccgatgagat gtgcggaaga tccggacaga ttgtataagt
      361 atgcaactaa gctgaagaaa aactgtaagg aaataactga taaggaattg gacaagaaaa
      421 tgaaggagct ggccgccgtc atgagcgacc ctgacctgga aactgagact atgtgcctcc
      481 acgacgacga gtcgtgtcgc tacgaagggc aagtcgctgt ttaccaggat gtatacgcgg
      541 ttgacggacc gacaagtctc tatcaccaag ccaataaggg agttagagtc gcctactgga
      601 taggctttga caccacccct tttatgttta agaacttggc tggagcatat ccatcatact
      661 ctaccaactg ggccgacgaa accgtgttaa cggctcgtaa cataggccta tgcagctctg
      721 acgttatgga gcggtcacgt agagggatgt ccattcttag aaagaagtat ttgaaaccat
      781 ccaacaatgt tctattctct gttggctcga ccatctacca cgagaagagg gacttactga
      841 ggagctggca cctgccgtct gtatttcact tacgtggcaa gcaaaattac acatgtcggt
      901 gtgagactat agttagttgc gacgggtacg tcgttaaaag aatagctatc agtccaggcc
      961 tgtatgggaa gccttcaggc tatgctgcta cgatgcaccg cgagggattc ttgtgctgca
     1021 aagtgacaga cacattgaac ggggagaggg tctcttttcc cgtgtgcacg tatgtgccag
     1081 ctacattgtg tgaccaaatg actggcatac tggcaacaga tgtcagtgcg gacgacgcgc
     1141 aaaaactgct ggttgggctc aaccagcgta tagtcgtcaa cggtcgcacc cagagaaaca
     1201 ccaataccat gaaaaattac cttttgcccg tagtggccca ggcatttgct aggtgggcaa
     1261 aggaatataa ggaagatcaa gaagatgaaa ggccactagg actacgagat agacagttag
     1321 tcatggggtg ttgttgggct tttagaaggc acaagataac atctatttat aagcgcccgg
     1381 atacccaaac catcatcaaa gtgaacagcg atttccactc attcgtgctg cccaggatag
     1441 gcagtaacac attggagatc gggctgagaa caagaatcag gaaaatgtta gaggagcaca
     1501 aggagccgtc acctctcatt accgccgagg acgtacaaga agctaagtgc gcagccgatg
     1561 aggctaagga ggtgcgtgaa gccgaggagt tgcgcgcagc tctaccacct ttggcagctg
     1621 atgttgagga gcccactctg gaggcagacg tcgacttgat gttacaagag gctggggccg
     1681 gctcagtgga gacacctcgt ggcttgataa aggttaccag ctacgatggc gaggacaaga
     1741 tcggctctta cgctgtgctt tctccgcagg ctgtactcaa gagtgaaaaa ttatcttgca
     1801 tccaccctct cgctgaacaa gtcatagtga taacacactc tggccgaaaa gggcgttatg
     1861 ccgtggaacc ataccatggt aaagtagtgg tgccagaggg acatgcaata cccgtccagg
     1921 actttcaagc tctgagtgaa agtgccacca ttgtgtacaa cgaacgtgag ttcgtaaaca
     1981 ggtacctgca ccatattgcc acacatggag gagcgctgaa cactgatgaa gaatattaca
     2041 aaactgtcaa gcccagcgag cacgacggcg aatacctgta cgacatcgac aggaaacagt
     2101 gcgtcaagaa agaactagtc actgggctag ggctcacagg cgagctggtg gatcctccct
     2161 tccatgaatt cgcctacgag agtctgagaa cacgaccagc cgctccttac caagtaccaa
     2221 ccataggggt gtatggcgtg ccaggatcag gcaagtctgg catcattaaa agcgcagtca
     2281 ccaaaaaaga tctagtggtg agcgccaaga aagaaaactg tgcagaaatt ataagggacg
     2341 tcaagaaaat gaaagggctg gacgtcaatg ccagaactgt ggactcagtg ctcttgaatg
     2401 gatgcaaaca ccccgtagag accctgtata ttgacgaagc ttttgcttgt catgcaggta
     2461 ctctcagagc gctcatagcc attataagac ctaaaaaggc agtgctctgc ggggatccca
     2521 aacagtgcgg tttttttaac atgatgtgcc tgaaagtgca ttttaaccac gagatttgca
     2581 cacaagtctt ccacaaaagc atctctcgcc gttgcactaa atctgtgact tcggtcgtct
     2641 caaccttgtt ttacgacaaa aaaatgagaa cgacgaatcc gaaagagact aagattgtga
     2701 ttgacactac cggcagtacc aaacctaagc aggacgatct cattctcact tgtttcagag
     2761 ggtgggtgaa gcagttgcaa atagattaca aaggcaacga aataatgacg gcagctgcct
     2821 ctcaagggct gacccgtaaa ggtgtgtatg ccgttcggta caaggtgaat gaaaatcctc
     2881 tgtacgcacc cacctcagaa catgtgaacg tcctactgac ccgcacggag gaccgcatcg
     2941 tgtggaaaac actagccggc gacccatgga taaaaacact gactgccaag taccctggga
     3001 atttcactgc cacgatagag gagtggcaag cagagcatga tgccatcatg aggcacatct
     3061 tggagagacc ggaccctacc gacgtcttcc agaataaggc aaacgtgtgt tgggccaagg
     3121 ctttagtgcc ggtgctgaag accgctggca tagacatgac cactgaacaa tggaacactg
     3181 tggattattt tgaaacggac aaagctcact cagcagagat agtattgaac caactatgcg
     3241 tgaggttctt tggactcgat ctggactccg gtctattttc tgcacccact gttccgttat
     3301 ccattaggaa taatcactgg gataactccc cgtcgcctaa catgtacggg ctgaataaag
     3361 aagtggtccg tcagctctct cgcaggtacc cacaactgcc tcgggcagtt gccactggaa
     3421 gagtctatga catgaacact ggtacactgc gcaattatga tccgcgcata aacctagtac
     3481 ctgtaaacag aagactgcct catgctttag tcctccacca taatgaacac ccacagagtg
     3541 acttttcttc attcgtcagc aaattgaagg gcagaactgt cctggtggtc ggggaaaagt
     3601 tgtccgtccc aggcaaaatg gttgactggt tgtcagaccg gcctgaggct accttcagag
     3661 ctcggctgga tttaggcatc ccaggtgatg tgcccaaata tgacataata tttgttaatg
     3721 tgaggacccc atataaatac catcactatc agcagtgtga agaccatgcc attaagctta
     3781 gcatgttgac caagaaagct tgtctgcatc tgaatcccgg cggaacctgt gtcagcatag
     3841 gttatggtta cgctgacagg gccagcgaaa gcatcattgg tgctatagcg cggcagttca
     3901 agttttcccg ggtatgcaaa ccgaaatcct cacttgaaga gacggaagtt ctgtttgtat
     3961 tcattgggta cgatcgcaag gcccgtacgc acaattctta caagctttca tcaaccttga
     4021 ccaacattta tacaggttcc agactccacg aagccggatg tgcaccctca tatcatgtgg
     4081 tgcgagggga tattgccacg gccaccgaag gagtgattat aaatgctgct aacagcaaag
     4141 gacaacctgg cggaggggtg tgcggagcgc tgtataagaa attcccggaa agcttcgatt
     4201 tacagccgat cgaagtagga aaagcgcgac tggtcaaagg tgcagctaaa catatcattc
     4261 atgccgtagg accaaacttc aacaaagttt cggaggttga aggtgacaaa cagttggcag
     4321 aggcttatga gtccatcgct aagattgtca acgataacaa ttacaagtca gtagcgattc
     4381 cactgttgtc caccggcatc ttttccggga acaaagatcg actaacccaa tcattgaacc
     4441 atttgctgac agctttagac accactgatg cagatgtagc catatactgc agggacaaga
     4501 aatgggaaat gactctcaag gaagcagtgg ctaggagaga agcagtggag gagatatgca
     4561 tatccgacga ctcttcagtg acagaacctg atgcagagct ggtgagggtg catccgaaga
     4621 gttctttggc tggaaggaag ggctacagca caagcgatgg caaaactttc tcatatttgg
     4681 aagggaccaa gtttcaccag gcggccaagg atatagcaga aattaatgcc atgtggcccg
     4741 ttgcaacgga ggccaatgag caggtatgca tgtatatcct cggagaaagc atgagcagta
     4801 ttaggtcgaa atgccccgtc gaagagtcgg aagcctccac accacctagc acgctgcctt
     4861 gcttgtgcat ccatgccatg actccagaaa gagtacagcg cctaaaagcc tcacgtccag
     4921 aacaaattac tgtgtgctca tcctttccat tgccgaagta tagaatcact ggtgtgcaga
     4981 agatccaatg ctcccagcct atattgttct caccgaaagt gcctgcgtat attcatccaa
     5041 ggaagtatct cgtggaaaca ccaccggtag acgagactcc ggagccatcg gcagagaacc
     5101 aatccacaga ggggacacct gaacaaccac cacttataac cgaggatgag accaggacta
     5161 gaacgcctga gccgatcatc atcgaagagg aagaagagga tagcataagt ttgctgtcag
     5221 atggcccgac ccaccaggtg ctgcaagtcg aggcagacat tcacgggccg ccctctgtat
     5281 ctagctcatc ctggtccatt cctcatgcat ccgactttga tgtggacagt ttatccatac
     5341 ttgacaccct ggagggagct agcgtgacca gcggggcaac gtcagccgag actaactctt
     5401 acttcgcaaa gagtatggag tttctggcgc gaccggtgcc tgcgcctcga acagtattca
     5461 ggaaccctcc acatcccgct ccgcgcacaa gaacaccgtc acttgcaccc agcagggcct
     5521 gctcgagaac cagcctagtt tccaccccgc caggcgtgaa tagggtgatc actagagagg
     5581 agctcgaggc gcttaccccg tcacgcactc ctagcaggtc ggtctcgaga accagcctgg
     5641 tctccaaccc gccaggcgta aatagggtga ttacaagaga ggagtttgag gcgttcgtag
     5701 cacaacaaca atgacggttt gatgcgggtg catacatctt ttcctccgac accggtcaag
     5761 ggcatttaca acaaaaatca gtaaggcaaa cggtgctatc cgaagtggtg ttggagagga
     5821 ccgaattgga gatttcgtat gccccgcgcc tcgaccaaga aaaagaagaa ttactacgca
     5881 agaaattaca gttaaatccc acacctgcta acagaagcag ataccagtcc aggaaggtgg
     5941 agaacatgaa agccataaca gctagacgta ttctgcaagg cctagggcat tatttgaagg
     6001 cagaaggaaa agtggagtgc taccgaaccc tgcatcctgt tcctttgtat tcatctagtg
     6061 tgaaccgtgc cttttcaagc cccaaggtcg cagtggaagc ctgtaacgcc atgttgaaag
     6121 agaactttcc gactgtggct tcttactgta ttattccaga gtacgatgcc tatttggaca
     6181 tggttgacgg agcttcatgc tgcttagaca ctgccagttt ttgccctgca aagctgcgca
     6241 gctttccaaa gaaacactcc tatttggaac ccacaatacg atcggcagtg ccttcagcga
     6301 tccagaacac gctccagaac gtcctggcag ctgccacaaa aagaaattgc aatgtcacgc
     6361 aaatgagaga attgcccgta ttggattcgg cggcctttaa tgtggaatgc ttcaagaaat
     6421 atgcgtgtaa taatgaatat tgggaaacgt ttaaagaaaa ccccatcagg cttactgaag
     6481 aaaacgtggt aaattacatt accaaattaa aaggaccaaa agctgctgct ctttttgcga
     6541 agacacataa tttgaatatg ttgcaggaca taccaatgga caggtttgta atggacttaa
     6601 agagagacgt gaaagtgact ccaggaacaa aacatactga agaacggccc aaggtacagg
     6661 tgatccaggc tgccgatccg ctagcaacag cgtatctgtg cggaatccac cgagagctgg
     6721 ttaggagatt aaatgcggtc ctgcttccga acattcatac actgtttgat atgtcggctg
     6781 aagactttga cgctattata gccgagcact tccagcctgg ggattgtgtt ctggaaactg
     6841 acatcgcgtc gtttgataaa agtgaggacg acgccatggc tctgaccgcg ttaatgattc
     6901 tggaagactt aggtgtggac gcagagctgt tgacgctgat tgaggcggct ttcggcgaaa
     6961 tttcatcaat acatttgccc actaaaacta aatttaaatt cggagccatg atgaaatctg
     7021 gaatgttcct cacactgttt gtgaacacag tcattaacat tgtaatcgca agcagagtgt
     7081 tgagagaacg gctaaccgga tcaccatgtg cagcattcat tggagatgac aatatcgtga
     7141 aaggagtcaa atcggacaaa ttaatggcag acaggtgcgc cacctggttg aatatggaag
     7201 tcaagattat agatgctgtg gtgggcgaga aagcgcctta tttctgtgga gggtttattt
     7261 tgtgtgactc cgtgaccggc acagcgtgcc gtgtggcaga ccccctaaaa aggctgttta
     7321 agcttggcaa acctctggca gcagacgatg aacatgatga tgacaggaga agggcattgc
     7381 atgaagagtc aacacgctgg aaccgagtgg gtattctttc agagctgtgc aaggcagtag
     7441 aatcaaggta tgaaaccgta ggaacttcca tcatagttat ggccatgact actctagcta
     7501 gcagtgttaa atcattcagc tacctgagag gggcccctat aactctctac ggctaacctg
     7561 aatggactac gacatagtct agtccgccaa gtctagcata tgggcgcgcc ctcagcatcg
     7621 attcaattcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     7681 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     7741 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     7801 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     7861 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     7921 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     7981 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     8041 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     8101 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     8161 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     8221 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     8281 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     8341 gagctgtaca agtagtctag agtcgacccg ggcggccgca actaacttaa gctagcaacg
     8401 gtttccctct agcgggatca attccgcccc ccccccctaa cgttactggc cgaagccgct
     8461 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     8521 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     8581 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     8641 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     8701 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     8761 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     8821 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     8881 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     8941 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac catgaccgag
     9001 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     9061 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     9121 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     9181 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     9241 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     9301 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     9361 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     9421 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     9481 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     9541 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agaattggca
     9601 agctgcttac atagaactcg cggcgattgg catgccgcct taaaattttt attttatttt
     9661 ttcttttctt ttccgaatcg gattttgttt ttaatatttc aaaaaaaaaa aaaaaaaaaa
     9721 aaaacgcgtc gaggggaatt aattcttgaa gacgaaaggg ccaggtggca cttttcgggg
     9781 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9841 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9901 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9961 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    10021 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    10081 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
    10141 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10201 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10261 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10321 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10381 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    10441 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    10501 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    10561 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10621 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10681 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10741 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10801 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10861 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10921 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10981 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    11041 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
    11101 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    11161 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    11221 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    11281 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    11341 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    11401 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    11461 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
//
