LOCUS       62056_23285        8195 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1051..1408
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1482..1889
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="VTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTFNVG
                     WPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKPPPP
                     LPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1899..2273
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    2850..3411
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3413..4129
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     LTR             4276..4867
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(5566..5582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6057..6161
                     /label=AmpR promoter
     CDS             6162..7019
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7193..7781
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8069..8090
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8105..8135
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8143..8159
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8167..8183
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa agcatttgac
       61 ttgttctatg ccctaggggg cggggggaag ctaagccagc tttttttaac atttaaaatg
      121 ttaattccat tttaaatgca cagatgtttt tatttcataa gggtttcaat gtgcatgaat
      181 gctgcaatat tcctgttacc aaagctagta taaataaaaa tagataaacg tggaaattac
      241 ttagagtttc tgtcattaac gtttccttcc tcagttgaca acataaatgc gctgctgagc
      301 aagccagttt gcatctgtca ggatcaattt cccattatgc cagtcatatt aattactagt
      361 caattagttg atttttattt ttgacatata catgtgaatg aaagacccca cctgtaggtt
      421 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
      481 agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
      541 atctgtggta agcagttcct gccccgctca gggccaagaa cagatggaac agctgaatat
      601 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg ctcagggcca agaacagatg
      661 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
      721 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
      781 gcttctgttc gcgcgcttat gctccccgag ctcaataaaa gagcccacaa cccctcactc
      841 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
      901 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
      961 gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc
     1021 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
     1081 cgattgtcta gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta
     1141 gctctgtatc tggcggaccc gtggtggaac tgacgagttc ggaacacccg gccgcaaccc
     1201 tgggagacgt cccagggact tcgggggccg tttttgtggc ccgacctgag tcctaaaatc
     1261 ccgatcgttt aggactcttt ggtgcacccc ccttagagga gggatatgtg gttctggtag
     1321 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc
     1381 gaagccgcgc cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc tgtctgactg
     1441 tgtttctgta tttgtctgaa aatatgggcc cgggctagcc tgttaccact cccttaagtt
     1501 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     1561 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     1621 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     1681 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     1741 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     1801 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     1861 atccagccct cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg
     1921 cacccccgcc ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct
     1981 ctctccaagc tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg
     2041 cggcagccta ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg
     2101 acacagtgtg ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt
     2161 acacagtcct gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac
     2221 acgccgccca cgtgaaggct gccgaccccg ggggtggacc atcctctagg ctgccaccat
     2281 gggcaacgag gccagctacc ctctggagat gtgctcccac ttcgacgccg acgagatcaa
     2341 gcggctgggc aagcgcttca agaagctgga cctggacaac agcggcagcc tgagcgtgga
     2401 ggagtttatg tctctgcccg agctgcagca gaaccccctg gtgcagcgcg tgatcgacat
     2461 cttcgacacc gacggcaacg gcgaggtgga cttcaaggag ttcatcgagg gcgtgagcca
     2521 gttcagcgtg aagggcgaca aggagcagaa gctgcggttc gccttccgga tctacgatat
     2581 ggataaagat ggctatattt ctaatggcga gctgttccag gtgctgaaga tgatggtggg
     2641 caacaatacc aagctggccg atacccagct gcagcagatc gtggacaaga ccatcatcaa
     2701 cgccgacaag gacggcgacg gcagaatcag cttcgaggag ttctgtgccg tggtgggagg
     2761 cctggatatt cacaaaaaaa tggtggtgga cgtgtgaacg cgtacgtcgc gaccgcggac
     2821 atgtacagag ctcgagcggg atcaattccg ccccccccct aacgttactg gccgaagccg
     2881 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     2941 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     3001 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     3061 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3121 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3181 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3241 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3301 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta
     3361 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata atatggtgag
     3421 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3481 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3541 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3601 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3661 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3721 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3781 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3841 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     3901 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     3961 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4021 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4081 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aatccggatt
     4141 agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata
     4201 tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct
     4261 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     4321 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
     4381 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     4441 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     4501 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
     4561 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     4621 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     4681 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
     4741 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     4801 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     4861 tctttcacac atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat
     4921 taaatggcca tagtacttaa agttacattg gcttccttga aataaacatg gagtattcag
     4981 aatgtgtcat aaatatttct aattttaaga tagtatctcc attggctttc tactttttct
     5041 tttatttttt tttgtcctct gtcttccatt tgttgttgtt gttgtttgtt tgtttgtttg
     5101 ttggttggtt ggttaatttt tttttaaaga tcctacacta tagttcaagc tagactatta
     5161 gctactctgt aacccagggt gaccttgaag tcatgggtag cctgctgttt tagccttccc
     5221 acatctaaga ttacaggtat gagctatcat ttttggtata ttgattgatt gattgattga
     5281 tgtgtgtgtg tgtgattgtg tttgtgtgtg tgactgtgaa aatgtgtgta tgggtgtgtg
     5341 tgaatgtgtg tatgtatgtg tgtgtgtgag tgtgtgtgtg tgtgtgtgca tgtgtgtgtg
     5401 tgtgactgtg tctatgtgta tgactgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     5461 tgtgtgtgtg ttgtgaaaaa atattctatg gtagtgagag ccaacgctcc ggctcaggtg
     5521 tcaggttggt ttttgagaca gagtctttca cttagcttgg aattcactgg ccgtcgtttt
     5581 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     5641 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5701 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     5761 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5821 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5881 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5941 gtcatcaccg aaacgcgcga tgacgaaagg gcctcgtgat acgcctattt ttataggtta
     6001 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6061 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6121 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6181 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6241 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6301 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6361 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6421 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6481 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6541 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6601 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6661 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6721 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6781 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6841 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6901 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6961 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7021 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7081 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7141 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7261 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7321 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7561 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7801 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7861 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7921 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7981 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8041 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8101 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     8161 ttcacacagg aaacagctat gaccatgatt acgcc
//
