LOCUS       V013384                 9126 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013384
VERSION     V013384
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     198..348
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     CDS             complement(763..1437)
                     /label="mRFP1"
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     promoter        complement(1480..1683)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    complement(2046..2167)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(2445..2544)
                     /label="CMVd1 promoter"
                     /note="truncated human cytomegalovirus (CMV) immediate
                     early promoter that yields slightly reduced expression
                     relative to the full-length promoter"
     enhancer        complement(2649..2952)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3114..3533
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(3599..4933)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     repeat_region   6174..6276
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(6523..7111)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             7918..8709
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ttaattaatc ccttccagct ctctgcccct tttggattga agccaatatg ataatgaggg
       61 ggtggagttt gtgacgtggc gcgggcgtgg gaacggggcg ggtgacgtag tagtgtggcg
      121 gaagtgtgat gttgcaagtg tggcggaaca catgtaagcg acggatgtgg caaaagtgac
      181 gtttttggtg tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt ttaggcggat
      241 gttgtagtaa atttgggcgt aaccgagtaa gatttggcca ttttcgcggg aaaactgaat
      301 aagaggaagt gaaatctgaa taattttgtg ttactcatag cgcgtaatat ttgtctaggg
      361 ccgcggggac tttgaccgtt tacgtggaga ctcgcccagg tgtttttctc aggtgttttc
      421 cgcgttccgg gtcaaagttg gcgttttatt attatagtca gtcgagtcta gcaagctagc
      481 ttgatcgcgt taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      541 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      601 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      661 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctt
      721 gtggtatggc tgattatgat ctagagtcgc ggccaattct taggcgccgg tggagtggcg
      781 gccctcggcg cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
      841 cagcttgatg tcggtcttgt aggcgccggg cagctgcacg ggcttcttgg ccatgtaggt
      901 ggtcttgacc tcggcgtcgt agtggccgcc gtccttcagc ttcagcctca tcttgatctc
      961 gcccttcagg gcgccgtcct cggggtacat ccgctcggtg gaggcctccc agcccatggt
     1021 cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta
     1081 gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc
     1141 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg
     1201 gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac tggaactgag gggacaggat
     1261 gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc
     1321 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc
     1381 ctccatgcgc accttgaagc gcatgaactc cttgatgacg tcctcggagg aggccatcgg
     1441 atcccggtag cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc
     1501 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     1561 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     1621 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     1681 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     1741 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     1801 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     1861 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     1921 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     1981 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     2041 cgcgttaaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2101 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2161 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2221 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc
     2281 tagatccggt ggatctgagt ccggacttgt acagctcgtc catgccgaga gtgatcccgg
     2341 cggcggtcac gaactccaga tcggatatct tatctagaag cttaggctcg agcggccgcg
     2401 tcgacggtac cagatctcta gcggatctga cggttcacta aaccagctct gcttatatag
     2461 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     2521 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     2581 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     2641 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     2701 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     2761 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     2821 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     2881 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     2941 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     3001 tattagacgt ttatcactgc tagaggatct gggcgtggtt aagggtggga aagaatatat
     3061 aaggtggggg tcttatgtag ttttgtatct gttttgcagc agccgccgcc gccatgagca
     3121 ccaactcgtt tgatggaagc attgtgagct catatttgac aacgcgcatg cccccatggg
     3181 ccggggtgcg tcagaatgtg atgggctcca gcattgatgg tcgccccgtc ctgcccgcaa
     3241 actctactac cttgacctac gagaccgtgt ctggaacgcc gttggagact gcagcctccg
     3301 ccgccgcttc agccgctgca gccaccgccc gcgggattgt gactgacttt gctttcctga
     3361 gcccgcttgc aagcagtgca gcttcccgtt catccgcccg cgatgacaag ttgacggctc
     3421 ttttggcaca attggattct ttgacccggg aacttaatgt cgtttctcag cagctgttgg
     3481 atctgcgcca gcaggtttct gccctgaagg cttcctcccc tcccaatgcg gtttaaaaca
     3541 taaataaaaa accagactct gtttggattt ggatcaagca agtgtcttgc tgtctttatt
     3601 taggggtttt gcgcgcgcgg taggcccggg accagcggtc tcggtcgttg agggtcctgt
     3661 gtattttttc caggacgtgg taaaggtgac tctggatgtt cagatacatg ggcataagcc
     3721 cgtctctggg gtggaggtag caccactgca gagcttcatg ctgcggggtg gtgttgtaga
     3781 tgatccagtc gtagcaggag cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc
     3841 tgattgccag gggcaggccc ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt
     3901 gcatacgtgg ggatatgaga tgcatcttgg actgtatttt taggttggct atgttcccag
     3961 ccatatccct ccggggattc atgttgtgca gaaccaccag cacagtgtat ccggtgcact
     4021 tgggaaattt gtcatgtagc ttagaaggaa atgcgtggaa gaacttggag acgcccttgt
     4081 gacctccaag attttccatg cattcgtcca taatgatggc aatgggccca cgggcggcgg
     4141 cctgggcgaa gatatttctg ggatcactaa cgtcatagtt gtgttccagg atgagatcgt
     4201 cataggccat ttttacaaag cgcgggcgga gggtgccaga ctgcggtata atggttccat
     4261 ccggcccagg ggcgtagtta ccctcacaga tttgcatttc ccacgctttg agttcagatg
     4321 gggggatcat gtctacctgc ggggcgatga agaaaacggt ttccggggta ggggagatca
     4381 gctgggaaga aagcaggttc ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa
     4441 tcacacctat taccgggtgc aactggtagt taagagagct gcagctgccg tcatccctga
     4501 gcaggggggc cacttcgtta agcatgtccc tgactcgcat gttttccctg accaaatccg
     4561 ccagaaggcg ctcgccgccc agcgatagca gttcttgcaa ggaagcaaag tttttcaacg
     4621 gtttgagacc gtccgccgta ggcatgcttt tgagcgtttg accaagcagt tccaggcggt
     4681 cccacagctc ggtcacctgc tctacggcat ctcgatccag catatctcct cgtttcgcgg
     4741 gttggggcgg ctttcgctgt acggcagtag tcggtgctcg tccagacggg ccagggtcat
     4801 gtctttccac gggcgcaggg tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc
     4861 tccgggctgc gcgctggcca gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg
     4921 ccggtcttcg ccctgcgcgt cggccaggta gcatttgacc atggtgtcat agtccagccc
     4981 ctccgcggcg tggcccttgg cgcgcagctt gcccttggag gaggcgccgc acgaggggca
     5041 gtgcagactt ttgagggcgt agagcttggg cgcgagaaat accgattccg gggagtaggc
     5101 atccgcgccg caggccccgc agacggtctc gcattccacg agccaggtga gctctggccg
     5161 ttcggggtca aaaaccaggt ttcccccatg ctttttgatg cgtttcttac ctctggtttc
     5221 catgagccgg tgtccacgct cggtgacgaa aaggctgtcc gtgtccccgt atacagactg
     5281 tttaaacgaa ttctataaaa tgcaaggtgc tgctcaaaaa atcaggcaaa gcctcgcgca
     5341 aaaaagaaag cacatcgtag tcatgctcat gcagataaag gcaggtaagc tccggaacca
     5401 ccacagaaaa agacaccatt tttctctcaa acatgtctgc gggtttctgc ataaacacaa
     5461 aataaaataa caaaaaaaca tttaaacatt agaagcctgt cttacaacag gaaaaacaac
     5521 ccttataagc ataagacgga ctacggccat gccggcgtga ccgtaaaaaa actggtcacc
     5581 gtgattaaaa agcaccaccg acagctcctc ggtcatgtcc ggagtcataa tgtaagactc
     5641 ggtaaacaca tcaggttgat tcatcggtca gtgctaaaaa gcgaccgaaa tagcccgggg
     5701 gaatacatac ccgcaggcgt agagacaaca ttacagcccc cataggaggt ataacaaaat
     5761 taataggaga gaaaaacaca taaacacctg aaaaaccctc ctgcctaggc aaaatagcac
     5821 cctcccgctc cagaacaaca tacagcgctt cacagcggca gcctaacagt cagccttacc
     5881 agtaaaaaag aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca
     5941 cagtgtaaaa aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg
     6001 gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag
     6061 ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt tcccacgtta cgtaacttcc
     6121 cattttaaga aaactacaat tcccaacaca tacaagttac tccgccctaa aacctacgtc
     6181 acccgccccg ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt
     6241 ggcttcaatc caaaataagg tatattattg atgatttaat taaggatccc ggtgtgaaat
     6301 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     6361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6961 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7441 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7501 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7561 gccagttaat agtttgcgca acgttgttga aaaaggatct tcacctagat ccttttcacg
     7621 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     7681 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     7741 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     7801 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctc gccgccaagg
     7861 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
     7921 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     7981 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     8041 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     8101 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     8161 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     8221 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     8281 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     8341 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     8401 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     8461 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     8521 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     8581 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     8641 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     8701 gagttcttct gaattttgtt aaaatttttg ttaaatcagc tcatttttta accaataggc
     8761 cgaaatcggc aacatccctt ataaatcaaa agaatagacc gcgatagggt tgagtgttgt
     8821 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     8881 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttgcg
     8941 gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     9001 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     9061 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg cgcgcttaat
     9121 gcgccg
//
