LOCUS       40924_48728        9363 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector RSFmobcat, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Katashkina JI, Kuvaeva TM, Skorokhodova AY, Biryukova IV, Golubeva 
            LI, Mashko SV.
  TITLE     Construction of Stably Maintained mob- Derivatives of RSF1010 
            Plasmid Lacking All the Known DNA Sequences Involved in the 
            Mobilization Process
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   Katashkina JI, Kuvaeva TM, Mashko SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2007) Ajinomoto-Genetika Research Institute, 1st 
            Dorozhny Pr., Moscow 117545, Russia
REFERENCE   3  (bases 1 to 9363)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2007) Ajinomoto-Genetika Research Institute, 1st Dorozhny 
            Pr., Moscow 117545, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..866
                     /codon_start=1
                     /gene="strA"
                     /product="streptomycin resistance protein A"
                     /label=strA
                     /protein_id="ABP62933.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            63..866
                     /gene="strA"
                     /label=strA
     CDS             866..1702
                     /codon_start=1
                     /gene="strB"
                     /product="streptomycin resistance protein B"
                     /label=strB
                     /protein_id="ABP62934.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            866..1702
                     /gene="strB"
                     /label=strB
     rep_origin      complement(2346..2740)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     promoter        3046..3148
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3149..3805
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    3838..3859
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3874..3904
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3912..3928
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3953..3975
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3983..5062
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             5087..6055
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6119..6331
                     /codon_start=1
                     /gene="orfE"
                     /product="unknown protein E"
                     /label=orfE
                     /note="hypothetical protein"
                     /protein_id="ABP62938.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     gene            6119..6331
                     /gene="orfE"
                     /label=orfE
     CDS             6333..6539
                     /codon_start=1
                     /gene="orfF"
                     /product="repressor protein F"
                     /label=orfF
                     /note="transcriptional regulator of repAC operon"
                     /protein_id="ABP62939.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     gene            6333..6539
                     /gene="orfF"
                     /label=orfF
     CDS             6569..7405
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7395..8243
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             8554..9342
                     /codon_start=1
                     /gene="sul"
                     /product="sulfonamide resistance protein"
                     /label=sul
                     /protein_id="ABP62942.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADVI
                     DLVRHPAIPTPRLFRPTQKSRVCAGAGRAQADGIPVSLDSYQPATQAYALSRGVAYLND
                     IRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGA
                     GIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRG
                     PGVSGPRHSLQSLPPPQVELTSSAHTSRAPCATGWRYWRR"
     gene            8554..9342
                     /gene="sul"
                     /label=sul
ORIGIN
        1 aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc
       61 cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag
      121 gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg
      181 cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag
      241 gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct
      301 tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca
      361 aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat
      421 gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca
      481 atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg cacgatcttt
      541 tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg
      601 tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
      661 cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
      721 tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
      781 tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
      841 gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
      901 cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
      961 gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
     1021 actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
     1081 tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
     1141 tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
     1201 tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
     1261 tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
     1321 ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
     1381 tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
     1441 tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
     1501 cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
     1561 ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
     1621 ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
     1681 ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
     1741 atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
     1801 acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
     1861 tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
     1921 cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
     1981 gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
     2041 aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
     2101 gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
     2161 taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
     2221 gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
     2281 agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
     2341 tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
     2401 aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
     2461 gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
     2521 tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
     2581 ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
     2641 gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
     2701 cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
     2761 gatcattcag cccggctcat agatctctga tgtccggcgg tgcttttgcc gttacgcacc
     2821 accccgtcag tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct
     2881 caaaaacacc atcatacact aaatcagtaa gttggcagca tcacccgacg cactttgcgc
     2941 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt
     3001 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag
     3061 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
     3121 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     3181 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     3241 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     3301 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     3361 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     3421 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     3481 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     3541 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     3601 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     3661 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     3721 tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     3781 ctgcgatgag tggcagggcg gggcgtaaga gctcgcgggc agtgagcgca acgcaattaa
     3841 tgtaagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     3901 aatgtgtgga attgtgagcg gataacaatt tcacacagga tctagaaata attttgttta
     3961 actttaagaa ggagatatac atatgaaacc agtaacgtta tacgatgtcg cagagtatgc
     4021 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     4081 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     4141 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     4201 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     4261 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     4321 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     4381 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     4441 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     4501 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     4561 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     4621 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
     4681 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     4741 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     4801 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     4861 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     4921 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     4981 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5041 ttcccgactg gaaagcgggc agtgagcgga tccggggggt ggcccgatga agaacgacag
     5101 gactttgcag gccataggcc gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg
     5161 cgtcagggac gccaccaccg gccagatgat gaaccgggaa tggtcagccg ccgaagtgct
     5221 ccagaacacg ccatggctca agcggatgaa tgcccagggc aatgacgtgt atatcaggcc
     5281 cgccgagcag gagcggcatg gtctggtgct ggtggacgac ctcagcgagt ttgacctgga
     5341 tgacatgaaa gccgagggcc gggagcctgc cctggtagtg gaaaccagcc cgaagaacta
     5401 tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa cttcgggggc agattgcccg
     5461 gacgctggcc agcgagtacg acgccgaccc ggccagcgcc gacagccgcc actatggccg
     5521 cttggcgggc ttcaccaacc gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg
     5581 ggtgctgctg cgtgaatcca agggcaagac cgccaccgct ggcccggcgc tggtgcagca
     5641 ggctggccag cagatcgagc aggcccagcg gcagcaggag aaggcccgca ggctggccag
     5701 cctcgaactg cccgagcggc agcttagccg ccaccggcgc acggcgctgg acgagtaccg
     5761 cagcgagatg gccgggctgg tcaagcgctt cggtgatgac ctcagcaagt gcgactttat
     5821 cgccgcgcag aagctggcca gccggggccg cagtgccgag gaaatcggca aggccatggc
     5881 cgaggccagc ccagcgctgg cagagcgcaa gcccggccac gaagcggatt acatcgagcg
     5941 caccgtcagc aaggtcatgg gtctgcccag cgtccagctt gcgcgggccg agctggcacg
     6001 ggcaccggca ccccgccagc gaggcatgga caggggcggg ccagatttca gcatgtagtg
     6061 cttgcgttgg tactcacgcc tgttatacta tgagtactca cgcacagaag ggggttttat
     6121 ggaatacgaa aaaagcgctt cagggtcggt ctacctgatc aaaagtgaca agggctattg
     6181 gttgcccggt ggctttggtt atacgtcaaa caaggccgag gctggccgct tttcagtcgc
     6241 tgatatggcc agccttaacc ttgacggctg caccttgtcc ttgttccgcg aagacaagcc
     6301 tttcggcccc ggcaagtttc tcggtgactg atatgaaaga ccaaaaggac aagcagaccg
     6361 gcgacctgct ggccagccct gacgctgtac gccaagcgcg atatgccgag cgcatgaagg
     6421 ccaaagggat gcgtcagcgc aagttctggc tgaccgacga cgaatacgag gcgctgcgcg
     6481 agtgcctgga agaactcaga gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac
     6541 caccaactgc ctgcaaagga ggcaatcaat ggctacccat aagcctatca atattctgga
     6601 ggcgttcgca gcagcgccgc caccgctgga ctacgttttg cccaacatgg tggccggtac
     6661 ggtcggggcg ctggtgtcgc ccggtggtgc cggtaaatcc atgctggccc tgcaactggc
     6721 cgcacagatt gcaggcgggc cggatctgct ggaggtgggc gaactgccca ccggcccggt
     6781 gatctacctg cccgccgaag acccgcccac cgccattcat caccgcctgc acgcccttgg
     6841 ggcgcacctc agcgccgagg aacggcaagc cgtggctgac ggcctgctga tccagccgct
     6901 gatcggcagc ctgcccaaca tcatggcccc ggagtggttc gacggcctca agcgcgccgc
     6961 cgagggccgc cgcctgatgg tgctggacac gctgcgccgg ttccacatcg aggaagaaaa
     7021 cgccagcggc cccatggccc aggtcatcgg tcgcatggag gccatcgccg ccgataccgg
     7081 gtgctctatc gtgttcctgc accatgccag caagggcgcg gccatgatgg gcgcaggcga
     7141 ccagcagcag gccagccggg gcagctcggt actggtcgat aacatccgct ggcagtccta
     7201 cctgtcgagc atgaccagcg ccgaggccga ggaatggggt gtggacgacg accagcgccg
     7261 gttcttcgtc cgcttcggtg tgagcaaggc caactatggc gcaccgttcg ctgatcggtg
     7321 gttcaggcgg catgacggcg gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag
     7381 caagggggtg ccccgtggtg aagcctaaga acaagcacag cctcagccac gtccggcacg
     7441 acccggcgca ctgtctggcc cccggcctgt tccgtgccct caagcggggc gagcgcaagc
     7501 gcagcaagct ggacgtgacg tatgactacg gcgacggcaa gcggatcgag ttcagcggcc
     7561 cggagccgct gggcgctgat gatctgcgca tcctgcaagg gctggtggcc atggctgggc
     7621 ctaatggcct agtgcttggc ccggaaccca agaccgaagg cggacggcag ctccggctgt
     7681 tcctggaacc caagtgggag gccgtcaccg ctgaatgcca tgtggtcaaa ggtagctatc
     7741 gggcgctggc aaaggaaatc ggggcagagg tcgatagtgg tggggcgctc aagcacatac
     7801 aggactgcat cgagcgcctt tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc
     7861 aggggtttcg gctgctgtcg gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg
     7921 ccctgaaccc cttgatcgcg caggccgtca tgggtggcgg ccagcatgtg cgcatcagca
     7981 tggacgaggt gcgggcgctg gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg
     8041 gctggatcga ccccggcaaa accggcaagg cttccataga taccttgtgc ggctatgtct
     8101 ggccgtcaga ggccagtggt tcgaccatgc gcaagcgccg ccagcgggtg cgcgaggcgt
     8161 tgccggagct ggtcgcgctg ggctggacgg taaccgagtt cgcggcgggc aagtacgaca
     8221 tcacccggcc caaggcggca ggctgacccc ccccactcta ttgtaaacaa gacattttta
     8281 tcttttatat tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc
     8341 tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca
     8401 taggccgctt tcctggcttt gcttccagat gtatgctctt ctgctcctgc agctaatgga
     8461 tcaccgcaaa caggttactc gcctggggat tccctttcga cccgagcatc cgtatgatac
     8521 tcatgctcga ttattattat tatagaagcc cccatgaata aatcgctcat cattttcggc
     8581 atcgtcaaca taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca
     8641 gccattgcgc aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctggtccgg
     8701 catccagcaa tcccgacgcc gcgcctgttt cgtccgacac agaaatcgcg cgtatgcgcc
     8761 ggtgctggac gcgctcaggc agatggcatt cccgtctcgc tcgacagtta tcaacccgcg
     8821 acgcaagcct atgccttgtc gcgtggtgtg gcctatctca atgatattcg cggttttcca
     8881 gacgctgcgt tctatccgca attggcgaaa tcatctgcca aactcgtcgt tatgcattcg
     8941 gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat ggatcacatt
     9001 gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa acgcaaccgc
     9061 cttgtccttg atcccggcat ggggtttttt ctgggggctg ctcccgaaac ctcgctctcg
     9121 gtgctggcgc ggttcgatga attgcggctg cgcttcgatt tgccggtgct tctgtctgtt
     9181 tcgcgcaaat cctttctgcg cgcgctcaca ggccgtggtc cgggggtgtc ggggccgcga
     9241 cactcgctgc agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc
     9301 cgcgcccctt gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc
     9361 gtt
//
