LOCUS       40924_48718        7212 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector RSFmob-I, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Katashkina JI, Kuvaeva TM, Skorokhodova AY, Biryukova IV, Golubeva 
            LI, Mashko SV.
  TITLE     Construction of Stably Maintained mob- Derivatives of RSF1010 
            Plasmid Lacking All the Known DNA Sequences Involved in the 
            Mobilization Process
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Katashkina JI, Kuvaeva TM, Mashko SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2007) Ajinomoto-Genetika Research Institute, 1st 
            Dorozhny Pr., Moscow 117545, Russia
REFERENCE   3  (bases 1 to 7212)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7212)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2007) Ajinomoto-Genetika Research Institute, 1st Dorozhny 
            Pr., Moscow 117545, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..866
                     /codon_start=1
                     /gene="strA"
                     /product="streptomycin resistance protein A"
                     /label=strA
                     /protein_id="ABP62952.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            63..866
                     /gene="strA"
                     /label=strA
     CDS             866..1702
                     /codon_start=1
                     /gene="strB"
                     /product="streptomycin resistance protein B"
                     /label=strB
                     /protein_id="ABP62953.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            866..1702
                     /gene="strB"
                     /label=strB
     rep_origin      complement(2346..2740)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     protein_bind    2810..2831
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2846..2876
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    2884..2900
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2936..3904
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             3968..4180
                     /codon_start=1
                     /gene="orfE"
                     /product="unknown protein E"
                     /label=orfE
                     /note="hypothetical protein"
                     /protein_id="ABP62955.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     gene            3968..4180
                     /gene="orfE"
                     /label=orfE
     CDS             4182..4388
                     /codon_start=1
                     /gene="orfF"
                     /product="repressor protein F"
                     /label=orfF
                     /note="transcriptional regulator of repAC operon"
                     /protein_id="ABP62956.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     gene            4182..4388
                     /gene="orfF"
                     /label=orfF
     CDS             4418..5254
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             5244..6092
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             6403..7191
                     /codon_start=1
                     /gene="sul"
                     /product="sulfonamide resistance protein"
                     /label=sul
                     /protein_id="ABP62959.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADVI
                     DLVRHPAIPTPRLFRPTQKSRVCAGAGRAQADGIPVSLDSYQPATQAYALSRGVAYLND
                     IRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGA
                     GIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRG
                     PGVSGPRHSLQSLPPPQVELTSSAHTSRAPCATGWRYWRR"
     gene            6403..7191
                     /gene="sul"
                     /label=sul
ORIGIN
        1 aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc
       61 cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag
      121 gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg
      181 cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag
      241 gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct
      301 tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca
      361 aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat
      421 gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca
      481 atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg cacgatcttt
      541 tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg
      601 tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
      661 cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
      721 tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
      781 tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
      841 gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
      901 cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
      961 gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
     1021 actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
     1081 tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
     1141 tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
     1201 tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
     1261 tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
     1321 ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
     1381 tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
     1441 tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
     1501 cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
     1561 ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
     1621 ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
     1681 ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
     1741 atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
     1801 acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
     1861 tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
     1921 cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
     1981 gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaattcttca
     2041 aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat
     2101 gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg
     2161 taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc
     2221 gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
     2281 agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
     2341 tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
     2401 aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
     2461 gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
     2521 tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
     2581 ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
     2641 gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
     2701 cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
     2761 gatcattcag cccggctcat agatctgcgg gcagtgagcg caacgcaatt aatgtaagtt
     2821 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     2881 gaattgtgag cggataacaa tttcacacag gatctaggat ccggggggtg gcccgatgaa
     2941 gaacgacagg actttgcagg ccataggccg acagctcaag gccatgggct gtgagcgctt
     3001 cgatatcggc gtcagggacg ccaccaccgg ccagatgatg aaccgggaat ggtcagccgc
     3061 cgaagtgctc cagaacacgc catggctcaa gcggatgaat gcccagggca atgacgtgta
     3121 tatcaggccc gccgagcagg agcggcatgg tctggtgctg gtggacgacc tcagcgagtt
     3181 tgacctggat gacatgaaag ccgagggccg ggagcctgcc ctggtagtgg aaaccagccc
     3241 gaagaactat caggcatggg tcaaggtggc cgacgccgca ggcggtgaac ttcgggggca
     3301 gattgcccgg acgctggcca gcgagtacga cgccgacccg gccagcgccg acagccgcca
     3361 ctatggccgc ttggcgggct tcaccaaccg caaggacaag cacaccaccc gcgccggtta
     3421 tcagccgtgg gtgctgctgc gtgaatccaa gggcaagacc gccaccgctg gcccggcgct
     3481 ggtgcagcag gctggccagc agatcgagca ggcccagcgg cagcaggaga aggcccgcag
     3541 gctggccagc ctcgaactgc ccgagcggca gcttagccgc caccggcgca cggcgctgga
     3601 cgagtaccgc agcgagatgg ccgggctggt caagcgcttc ggtgatgacc tcagcaagtg
     3661 cgactttatc gccgcgcaga agctggccag ccggggccgc agtgccgagg aaatcggcaa
     3721 ggccatggcc gaggccagcc cagcgctggc agagcgcaag cccggccacg aagcggatta
     3781 catcgagcgc accgtcagca aggtcatggg tctgcccagc gtccagcttg cgcgggccga
     3841 gctggcacgg gcaccggcac cccgccagcg aggcatggac aggggcgggc cagatttcag
     3901 catgtagtgc ttgcgttggt actcacgcct gttatactat gagtactcac gcacagaagg
     3961 gggttttatg gaatacgaaa aaagcgcttc agggtcggtc tacctgatca aaagtgacaa
     4021 gggctattgg ttgcccggtg gctttggtta tacgtcaaac aaggccgagg ctggccgctt
     4081 ttcagtcgct gatatggcca gccttaacct tgacggctgc accttgtcct tgttccgcga
     4141 agacaagcct ttcggccccg gcaagtttct cggtgactga tatgaaagac caaaaggaca
     4201 agcagaccgg cgacctgctg gccagccctg acgctgtacg ccaagcgcga tatgccgagc
     4261 gcatgaaggc caaagggatg cgtcagcgca agttctggct gaccgacgac gaatacgagg
     4321 cgctgcgcga gtgcctggaa gaactcagag cggcgcaggg cgggggtagt gaccccgcca
     4381 gcgcctaacc accaactgcc tgcaaaggag gcaatcaatg gctacccata agcctatcaa
     4441 tattctggag gcgttcgcag cagcgccgcc accgctggac tacgttttgc ccaacatggt
     4501 ggccggtacg gtcggggcgc tggtgtcgcc cggtggtgcc ggtaaatcca tgctggccct
     4561 gcaactggcc gcacagattg caggcgggcc ggatctgctg gaggtgggcg aactgcccac
     4621 cggcccggtg atctacctgc ccgccgaaga cccgcccacc gccattcatc accgcctgca
     4681 cgcccttggg gcgcacctca gcgccgagga acggcaagcc gtggctgacg gcctgctgat
     4741 ccagccgctg atcggcagcc tgcccaacat catggccccg gagtggttcg acggcctcaa
     4801 gcgcgccgcc gagggccgcc gcctgatggt gctggacacg ctgcgccggt tccacatcga
     4861 ggaagaaaac gccagcggcc ccatggccca ggtcatcggt cgcatggagg ccatcgccgc
     4921 cgataccggg tgctctatcg tgttcctgca ccatgccagc aagggcgcgg ccatgatggg
     4981 cgcaggcgac cagcagcagg ccagccgggg cagctcggta ctggtcgata acatccgctg
     5041 gcagtcctac ctgtcgagca tgaccagcgc cgaggccgag gaatggggtg tggacgacga
     5101 ccagcgccgg ttcttcgtcc gcttcggtgt gagcaaggcc aactatggcg caccgttcgc
     5161 tgatcggtgg ttcaggcggc atgacggcgg ggtgctcaag cccgccgtgc tggagaggca
     5221 gcgcaagagc aagggggtgc cccgtggtga agcctaagaa caagcacagc ctcagccacg
     5281 tccggcacga cccggcgcac tgtctggccc ccggcctgtt ccgtgccctc aagcggggcg
     5341 agcgcaagcg cagcaagctg gacgtgacgt atgactacgg cgacggcaag cggatcgagt
     5401 tcagcggccc ggagccgctg ggcgctgatg atctgcgcat cctgcaaggg ctggtggcca
     5461 tggctgggcc taatggccta gtgcttggcc cggaacccaa gaccgaaggc ggacggcagc
     5521 tccggctgtt cctggaaccc aagtgggagg ccgtcaccgc tgaatgccat gtggtcaaag
     5581 gtagctatcg ggcgctggca aaggaaatcg gggcagaggt cgatagtggt ggggcgctca
     5641 agcacataca ggactgcatc gagcgccttt ggaaggtatc catcatcgcc cagaatggcc
     5701 gcaagcggca ggggtttcgg ctgctgtcgg agtacgccag cgacgaggcg gacgggcgcc
     5761 tgtacgtggc cctgaacccc ttgatcgcgc aggccgtcat gggtggcggc cagcatgtgc
     5821 gcatcagcat ggacgaggtg cgggcgctgg acagcgaaac cgcccgcctg ctgcaccagc
     5881 ggctgtgtgg ctggatcgac cccggcaaaa ccggcaaggc ttccatagat accttgtgcg
     5941 gctatgtctg gccgtcagag gccagtggtt cgaccatgcg caagcgccgc cagcgggtgc
     6001 gcgaggcgtt gccggagctg gtcgcgctgg gctggacggt aaccgagttc gcggcgggca
     6061 agtacgacat cacccggccc aaggcggcag gctgaccccc cccactctat tgtaaacaag
     6121 acatttttat cttttatatt caatggctta ttttcctgct aattggtaat accatgaaaa
     6181 ataccatgct cagaaaaggc ttaacaatat tttgaaaaat tgcctactga gcgctgccgc
     6241 acagctccat aggccgcttt cctggctttg cttccagatg tatgctcttc tgctcctgca
     6301 gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac ccgagcatcc
     6361 gtatgatact catgctcgat tattattatt atagaagccc ccatgaataa atcgctcatc
     6421 attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg gtatctggcg
     6481 ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga tgtgatcgac
     6541 ctggtccggc atccagcaat cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc
     6601 gtatgcgccg gtgctggacg cgctcaggca gatggcattc ccgtctcgct cgacagttat
     6661 caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc
     6721 ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt
     6781 atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg cacccgctgg cgacatcatg
     6841 gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa
     6901 cgcaaccgcc ttgtccttga tcccggcatg gggttttttc tgggggctgc tcccgaaacc
     6961 tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt
     7021 ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag gccgtggtcc gggggtgtcg
     7081 gggccgcgac actcgctgca gagcttgccg ccgccgcagg tggagctgac ttcatccgca
     7141 cacacgagcc gcgccccttg cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg
     7201 caagaattcg tt
//
