LOCUS       62056_23260        7361 bp DNA     circular SYN 18-MAY-2019
DEFINITION  Cloning vector RS540-AAV-ErbB-RASER1C-OFPBidBH3, complete sequence.
ACCESSION   MK801287
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Chung HK, Zou X, Bajar BT, Brand VR, Huo Y, Alcudia JF, Ferrell JE 
            Jr., Lin MZ.
  TITLE     A compact synthetic pathway rewires cancer signaling to therapeutic 
            effector release
  JOURNAL   Science 364 (6439) (2019)
  PUBMED    31048459
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Chung HK, Lin MZ.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2019) Bioengineering, Stanford University, 269 
            Campus Drive, CCRS2100, Stanford, CA 94305, United States
REFERENCE   3  (bases 1 to 7361)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science 364
            (6439) (2019)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-APR-2019) Bioengineering, Stanford University, 269 Campus Drive,
            CCRS2100, Stanford, CA 94305, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=L-ITR
                     /note="L-ITR"
     misc_feature    137..142
                     /note="XbaI; Geneious type: restriction; XbaI"
     misc_feature    143..148
                     /label=XhoI
                     /note="XhoI"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     731..770
                     /label=p2006 Nhe1 TM for
                     /note="p2006 Nhe1 TM for"
     misc_feature    758..762
                     /label=nonstandard type: Editing History Replacement; C
     CDS             928..954
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     primer_bind     1068..1108
                     /label=p1940 TM Bsp1047I for
                     /note="p1940 TM Bsp1047I for"
     CDS             1180..1689
                     /label=CIBN
                     /note="N-terminal portion of the CIB1 transcription factor
                     from Arabidopsis thaliana"
     misc_feature    complement(1690..1719)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse; p1853
     primer_bind     1705..1741
                     /label=p1727
                     /note="p1727"
     misc_feature    1723..2106
                     /label=nonstandard type: gBLOCK; HKC gBLOCK-5 VAV1
     misc_feature    1744..2076
                     /label=nonstandard type: SH2; VAV1
     misc_feature    2134..2151
                     /label=nonstandard type: polylinker; L6
     misc_feature    2188..2220
                     /label=nonstandard type: Substrate; substrate 4
     misc_feature    complement(2205..2245)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse; p1942 sub4 
                     AscI
                     Bid rev
     misc_feature    2230..2316
                     /label=nonstandard type: misc; Bid
     CDS             2317..3045
                     /label=mCardinal
                     /note="far-red fluorescent protein derived from mNeptune,
                     with further red-shifted spectra"
     misc_feature    3052..3093
                     /label=nonstandard type: epitope; V5
     primer_bind     3052..3093
                     /label=p1291
                     /note="p1291"
     misc_feature    3103..3165
                     /note="T2A peptide from Thosea asigna virus capsid protein"
     misc_feature    3166..3723
                     /label=Shc-PTB
                     /note="Shc-PTB"
     misc_feature    complement(3192..3228)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse; p1856 SmaI 
                     PTB
                     rev
     misc_feature    3529..3549
                     /label=HIF1a
                     /note="HIF1a"
     CDS             3727..3750
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    3787..4365
                     /label=nonstandard type: Protease; NS3Tprotease
     misc_feature    3946..3948
                     /label=nonstandard type: misc; T54
     primer_bind     4113..4145
                     /label=p1278
                     /note="p1278"
     misc_feature    complement(4131..4161)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse; p1502 MreI 
                     rev
     misc_feature    complement(4344..4387)
                     /direction=LEFT
                     /label=nonstandard type: primer_bind_reverse
     primer_bind     4366..4403
                     /label=p1992 EcoRI EcoRV polyA
                     /note="p1992 EcoRI EcoRV polyA"
     misc_feature    4369..4374
                     /label=EcoRV
                     /note="EcoRV"
     polyA_signal    4408..4615
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4616..4623
                     /label=NotI
                     /note="NotI"
     repeat_region   4624..4764
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4839..5294
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5576..5680
                     /label=AmpR promoter
     CDS             5681..6538
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6712..7300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctctc tggctaacta ccggtgctag cgaccatgca cctgagaatc
      781 cacgcgagac ggagccctcc tcgccggccg gcctggacgc ttgggatctg gttcctgttc
      841 tggggatgta tcgtcagctc tgtatggagt caattgtctt ctaatgtagc ttcctcctcc
      901 tccacctctt cctcgccggg gtctcactac ccatacgatg tgccagatta cgccaacccc
      961 acggagccgg gaatcagacg ggttccgggg gcctcagagg tgatccggga gtcgagcagc
     1021 acaacaggga tggtcgtcgg cattgtggct gctgccgccc tctgcatctt gatcctcctg
     1081 tacgccatgt acaagtacag gaacagggac gagggctcgg gctcgacctc gggctcgggc
     1141 agcggtggct ctggaggttc tggtggcagc tctggaggta tgaatggagc tataggaggt
     1201 gaccttttgc tcaattttcc tgacatgtcg gtcctagagc gccaaagggc tcacctcaag
     1261 tacctcaatc ccacctttga ttctcctctc gccggcttct ttgccgattc ttcaatgatt
     1321 accggcggcg agatggacag ctatctttcg actgccggtt tgaatcttcc gatgatgtac
     1381 ggtgagacga cggtggaagg tgattcaaga ctctcaattt cgccggaaac gacgcttggg
     1441 actggaaatt tcaaggcagc gaagtttgat acagagacta aggattgtaa tgaggcggcg
     1501 aagaagatga cgatgaacag agatgaccta gtagaagaag gagaagaaga gaagtcgaaa
     1561 ataacagagc aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca caaagccaag
     1621 aaagaagaga acaatttctc taatgattca tctaaagtga cgaaggaatt ggagaaaacg
     1681 gattatattc atagcggtag tggcggcggc agtgggggct ctggtgggtc aggtggcagt
     1741 ggcggccctc ctcaggacct gtctgttcat ctctggtacg caggccccat ggagcgggca
     1801 ggggcagaga gcatcctggc caaccgctcg gacgggactt tcttggtgcg gcagagggtg
     1861 aaggatgcag cagaatttgc catcagcatt aaatataacg tcgaggtcaa gcacattaaa
     1921 atcatgacag cagaaggact gtaccggatc acagagaaaa aggctttccg ggggcttacg
     1981 gagctggtgg agttttacca gcagaactct ctaaaggatt gcttcaagtc tctggacacc
     2041 accttgcagt tccccttcaa ggagcctgaa aagagaggat ctagaggggg atctggtgga
     2101 tcagggggta gcgggggttc tggcggaccg gcaggcagta gcggaagcag tattatacct
     2161 gacagggagg ttctctacca ggagttcgat gagatggaag agtgctctca gcacctcccc
     2221 ggcgcgccta tggaagatat tattcggaac attgctcggc atctggcggt tggcgataac
     2281 ctcgacagat caatctgggg cggctctggc ggctccgtga gcaagggcga ggagctgatc
     2341 aaggagaaca tgcacatgaa gctgtacatg gaaggcaccg tgaacaacca ccacttcaag
     2401 tgcaccaccg aaggggaggg caagccctac gagggcaccc agacccagag gattaaggtg
     2461 gtggagggag gccccctgcc gttcgcattc gacatcctgg ccacctgctt tatgtacggg
     2521 agcaagacct tcatcaacca cacccagggc atccccgatt tctttaagca gtccttccct
     2581 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct taccgttacc
     2641 caggacacca gcctccagga cggctgcttg atctacaacg tcaagctcag aggggtgaac
     2701 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc caccaccgag
     2761 accctgtacc ccgctgacgg cggcctggaa ggcagatgcg acatggccct gaagctcgtg
     2821 ggcgggggcc acctgcactg caacctgaag accacataca gatccaagaa acccgctaag
     2881 aacctcaaga tgcccggcgt ctactttgtg gaccgcagac tggaaagaat caaggaggcc
     2941 gacaatgaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     3001 agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagggatc cggtaaacca
     3061 atccccaatc ctctgctggg tcttgatagc accttaatta acggcagtgg agagggcaga
     3121 ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaatggg caagccactg
     3181 catcccaacg acaaagtcat gggacccggg gtttcctact tggttcggta catgggttgt
     3241 gtggaggtcc tccagtcaat gcgtgccctg gacttcaaca cccggactca ggtcaccagg
     3301 gaggccatca gtctggtgtg tgaggctgtg ccgggtgcta agggggcgac aaggaggaga
     3361 aagccctgta gccgcccgct cagctctatc ctggggagga gtaacctgaa atttgctgga
     3421 atgccaatca ctctcaccgt ctccaccagc agcctcaacc tcatggccgc agactgcaaa
     3481 cagatcatcg ccaaccacca catgcaatct atctcatttg catccggcat gttagctccc
     3541 tatatcccag agtatgtcgc ctatgttgcc aaagaccctg tgaatcagag agcctgccac
     3601 attctggagt gtcccgaagg gcttgcccag gatgtcatca gcaccattgg ccaggccttc
     3661 gagttgcgct tcaaacaata cctcagggac atcgagcagg tgccccagca gcccaccctt
     3721 aagatggatt acaaggatga cgacgataag ggctcttccg ggacaggctc cggatccggc
     3781 actagtgcgc ccatcacggc gtacgcccag cagacgagag gcctcctagg gtgtataatc
     3841 accagcctga ctggccggga caaaaaccaa gtggagggtg aggtccagat cgtgtcaact
     3901 gctacccaaa ccttcctggc aacgtgcatc aatggggtat gctggactgt ctaccacggg
     3961 gccggaacga ggaccatcgc atcacccaag ggtcctgtca tccagatgta taccaatgtg
     4021 gaccaagacc ttgtgggctg gcccgctcct caaggttccc gctcattgac accctgtacc
     4081 tgcggctcct cggaccttta cctggtcacg aggcacgccg atgtcattcc cgtgcgccgg
     4141 cgaggtgata gcaggggtag cctgctttcg ccccggccca tttcctactt gaaaggctcc
     4201 tcggggggtc cgctgttgtg ccccgcggga cacgccgtgg gcctattcag ggccgcggtg
     4261 tgcacccgtg gagtggctaa agcggtggac tttatccctg tggagaacct agagacaacc
     4321 atgagatccc cggtgttctc ggacaattcc tccccaccag cggtgtgaga tatctaagaa
     4381 ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4441 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4501 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4561 tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct ggggagcggc
     4621 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     4681 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     4741 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     4801 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4861 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4921 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4981 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5041 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5101 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5161 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     5221 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5281 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5341 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5401 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5461 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5521 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5581 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5641 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5701 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5761 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5821 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5881 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5941 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6001 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6061 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6121 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6181 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6241 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6301 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6361 gtttattgct gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact
     6421 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6481 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6541 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6601 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6661 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6721 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6781 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6841 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6901 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6961 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7021 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7081 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7141 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7201 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7261 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7321 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
