LOCUS       V015478                 9820 bp    DNA     circular SYN 18-MAY-2019
DEFINITION  Exported.
ACCESSION   V015478
VERSION     V015478
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Chung HK, Zou X, Bajar BT, Brand VR, Huo Y, Alcudia JF, Ferrell JE
            Jr., Lin MZ.
  TITLE     A compact synthetic pathway rewires cancer signaling to therapeutic
            effector release
  JOURNAL   Science 364 (6439) (2019)
   PUBMED   31048459
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Chung HK, Lin MZ.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2019) Bioengineering, Stanford University, 269
            Campus Drive, CCRS2100, Stanford, CA 94305, United States
REFERENCE   3  (bases 1 to 9820)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Science 364
            (6439) (2019)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-APR-2019) Bioengineering, Stanford University, 269 Campus Drive,
            CCRS2100, Stanford, CA 94305, United States"
FEATURES             Location/Qualifiers
     source          1..9820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      12..564
                     /regulatory_class="promoter"
     misc_feature    67..354
                     /label="CAG_enhancer"
                     /note="CAG_enhancer"
     misc_feature    521..541
                     /label="CMV_fwd_primer"
                     /note="CMV_fwd_primer"
     LTR             584..764
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1435..1668
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1853..1897
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2046..2087
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2195..2312
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2371..2684
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das
                     et al., 1988)"
     primer_bind     2701..2717
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2759..2792
                     /label="loxP"
                     /note="loxP"
     enhancer        2908..3211
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3212..3415
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     3416..3465
                     /label="p1852 NheI kozak TM"
                     /note="p1852 NheI kozak TM"
     misc_feature    3448..3449
                     /label="nonstandard type: Editing History Replacement; GGT"
     CDS             3612..3638
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3864..4373
                     /label="CIBN"
                     /note="N-terminal portion of the CIB1 transcription factor
                     from Arabidopsis thaliana"
     misc_feature    complement(4374..4403)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1853"
     primer_bind     4389..4425
                     /label="p1727"
                     /note="p1727"
     misc_feature    4428..4760
                     /label="nonstandard type: SH2; VAV1"
     misc_feature    4818..4835
                     /label="nonstandard type: polylinker; L6"
     misc_feature    4872..4904
                     /label="nonstandard type: Substrate; substrate 4"
     misc_feature    4874..4904
                     /label="nonstandard type: Editing History Replacement;
                     AGATGTCGTGCCATGCTCAATGGGCTCG"
     CDS             4914..5564
                     /label="mKO2"
                     /note="monomeric Kusabira-Orange 2 fluorescent protein"
     misc_feature    5577..5618
                     /label="nonstandard type: epitope; V5"
     primer_bind     5577..5618
                     /label="p1291"
                     /note="p1291"
     misc_feature    5620..5690
                     /label="nonstandard type: Editing History Replacement;
                     AAAGCGGCCGCGTCGACGGGCCCGCGGAATTCCGCCCCCCCC
                     CCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGT
                     GTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGC
                     CCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCC
                     AAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTT
                     GAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGA
                     CAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAA
                     CCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAA
                     GCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGA
                     TCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTA
                     GGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGCC
                     ACAACC"
     misc_feature    5628..5690
                     /note="T2A peptide from Thosea asigna virus capsid protein"
     misc_feature    complement(5637..5690)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1869 T2A
                     rev"
     primer_bind     5646..5705
                     /label="p1867 T2A PTB"
                     /note="p1867 T2A PTB"
     misc_feature    complement(5717..5753)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1856 SmaI
                     PTB rev"
     misc_feature    6054..6074
                     /label="HIF1a"
                     /note="HIF1a"
     CDS             6252..6275
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    6312..6890
                     /label="nonstandard type: Protease; NS3Tprotease"
     misc_feature    6471..6473
                     /label="nonstandard type: Editing History Replacement; GCA"
     primer_bind     6638..6670
                     /label="p1278"
                     /note="p1278"
     misc_feature    complement(6656..6686)
                     /direction=LEFT
                     /label="nonstandard type: primer_bind_reverse; p1502 MreI
                     rev"
     protein_bind    complement(6921..6954)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7010..7598
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(7601..7617)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    7783..7804
                     /label="U3PPT"
                     /note="U3PPT"
     misc_feature    7783..7798
                     /label="cPPT"
                     /note="cPPT"
     misc_feature    7840..8020
                     /label="truncHIV-1_3_LTR"
                     /note="truncHIV-1_3_LTR"
     rep_origin      complement(8082..8670)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8844..9701)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9702..9806)
                     /label="AmpR promoter"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga
     1321 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1381 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1441 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1501 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1561 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1621 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1681 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1741 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1801 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1861 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1921 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1981 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2041 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2101 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2161 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2221 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2281 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2341 cagtttggtt agtaccgggc ccgctctaga gatccgacgc cgccatctct aggcccgcgc
     2401 cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg
     2461 catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt
     2521 tctttttaat actagctaca ttttacatga taggcttgga tttctataag agatacaaat
     2581 actaaattat tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt
     2641 gtgtgttttg agactataaa tatcccttgg agaaaagcct tgttaacgcg cggtgaccct
     2701 cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat
     2761 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggccgc
     2821 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     2881 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2941 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     3001 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     3061 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     3121 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     3181 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     3241 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     3301 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     3361 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
     3421 agtgaaccgt cagatccgct agctccacca tgcacctgag aatccacgcg agacggagcc
     3481 ctcctcgccg gccggcctgg acgcttggga tctggttcct gttctgggga tgtatcgtca
     3541 gctctgtatg gagtcaattg tcttctaatg tagcttcctc ctcctccacc tcttcctcgc
     3601 cggggtctca ctacccatac gatgtgccag attacgccaa ccccacggag ccgggaatca
     3661 gacgggttcc gggggcctca gaggtgatcc gggagtcgag cagcacaaca gggatggtcg
     3721 tcggcattgt ggctgctgcc gccctctgca tcttgatcct cctgtacgcc atgtacaagt
     3781 acaggaacag ggacgagggc tcgggctcga cctcgggctc gggcagcggt ggctctggag
     3841 gttctggtgg cagctctgga ggtatgaatg gagctatagg aggtgacctt ttgctcaatt
     3901 ttcctgacat gtcggtccta gagcgccaaa gggctcacct caagtacctc aatcccacct
     3961 ttgattctcc tctcgccggc ttctttgccg attcttcaat gattaccggc ggcgagatgg
     4021 acagctatct ttcgactgcc ggtttgaatc ttccgatgat gtacggtgag acgacggtgg
     4081 aaggtgattc aagactctca atttcgccgg aaacgacgct tgggactgga aatttcaagg
     4141 cagcgaagtt tgatacagag actaaggatt gtaatgaggc ggcgaagaag atgacgatga
     4201 acagagatga cctagtagaa gaaggagaag aagagaagtc gaaaataaca gagcaaaaca
     4261 atgggagcac aaaaagcatc aagaagatga aacacaaagc caagaaagaa gagaacaatt
     4321 tctctaatga ttcatctaaa gtgacgaagg aattggagaa aacggattat attcatagcg
     4381 gtagtggcgg cggcagtggg ggctctggtg ggtcaggtgg cagtggcggc cctcctcagg
     4441 acctgtctgt tcatctctgg tacgcaggcc ccatggagcg ggcaggggca gagagcatcc
     4501 tggccaaccg ctcggacggg actttcttgg tgcggcagag ggtgaaggat gcagcagaat
     4561 ttgccatcag cattaaatat aacgtcgagg tcaagcacat taaaatcatg acagcagaag
     4621 gactgtaccg gatcacagag aaaaaggctt tccgggggct tacggagctg gtggagtttt
     4681 accagcagaa ctctctaaag gattgcttca agtctctgga caccaccttg cagttcccct
     4741 tcaaggagcc tgaaaagaga ggatctagag ggggatctgg tggatcaggg ggtagcgggg
     4801 gttctggcgg accggcaggc agtagcggaa gcagtattat acctgacagg gaggttctct
     4861 accaggagtt cgatgagatg gaagagtgct ctcagcacct ccccggcgcg cctatggtga
     4921 gcgtgatcaa gcccgagatg aagatgaggt actacatgga cggctccgtc aatgggcatg
     4981 agttcacaat cgagggtgag ggcacaggca gaccttacga gggacatcag gagatgacac
     5041 tgcgcgtcac aatggccgag ggcgggccaa tgcctttcgc cttcgacctg gtgtcccacg
     5101 tgttctgtta cggccacaga gtttttacca agtacccaga agagatccca gactatttca
     5161 agcaggcctt tcctgagggc ctgtcctggg agaggtccct ggagttcgag gacggcggct
     5221 ccgcctccgt gagcgcccac atcagcctga ggggcaacac cttctaccac aagtccaagt
     5281 tcaccggcgt gaacttcccc gccgacggcc ccatcatgca gaaccagagc gtggactggg
     5341 agccctccac cgagaagatc accgccagcg acggcgtgct gaagggcgac gtgaccatgt
     5401 acctgaagct ggagggcggc ggcaaccaca agtgccagat gaagaccacc tacaaggccg
     5461 ccaaggagat cctggagatg cccggcgacc actacatcgg ccacaggctg gtgaggaaga
     5521 ccgagggcaa catcaccgag caggtggagg acgccgtggc ccaccttaag ggatccggta
     5581 aaccaatccc caatcctctg ctgggtcttg atagcacctt aattaacggc agtggagagg
     5641 gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca atgggcaagc
     5701 cactgcatcc caacgacaaa gtcatgggac ccggggtttc ctacttggtt cggtacatgg
     5761 gttgtgtgga ggtcctccag tcaatgcgtg ccctggactt caacacccgg actcaggtca
     5821 ccagggaggc catcagtctg gtgtgtgagg ctgtgccggg tgctaagggg gcgacaagga
     5881 ggagaaagcc ctgtagccgc ccgctcagct ctatcctggg gaggagtaac ctgaaatttg
     5941 ctggaatgcc aatcactctc accgtctcca ccagcagcct caacctcatg gccgcagact
     6001 gcaaacagat catcgccaac caccacatgc aatctatctc atttgcatcc ggcatgttag
     6061 ctccctatat cccagagtat gtcgcctatg ttgccaaaga ccctgtgaat cagagagcct
     6121 gccacattct ggagtgtccc gaagggcttg cccaggatgt catcagcacc attggccagg
     6181 ccttcgagtt gcgcttcaaa caatacctca gggacatcga gcaggtgccc cagcagccca
     6241 cccttaagat ggattacaag gatgacgacg ataagggctc ttccgggaca ggctccggat
     6301 ccggcactag tgcgcccatc acggcgtacg cccagcagac gagaggcctc ctagggtgta
     6361 taatcaccag cctgactggc cgggacaaaa accaagtgga gggtgaggtc cagatcgtgt
     6421 caactgctac ccaaaccttc ctggcaacgt gcatcaatgg ggtatgctgg actgtctacc
     6481 acggggccgg aacgaggacc atcgcatcac ccaagggtcc tgtcatccag atgtatacca
     6541 atgtggacca agaccttgtg ggctggcccg ctcctcaagg ttcccgctca ttgacaccct
     6601 gtacctgcgg ctcctcggac ctttacctgg tcacgaggca cgccgatgtc attcccgtgc
     6661 gccggcgagg tgatagcagg ggtagcctgc tttcgccccg gcccatttcc tacttgaaag
     6721 gctcctcggg gggtccgctg ttgtgccccg cgggacacgc cgtgggccta ttcagggccg
     6781 cggtgtgcac ccgtggagtg gctaaagcgg tggactttat ccctgtggag aacctagaga
     6841 caaccatgag atccccggtg ttctcggaca attcctcccc accagcggtg tgagtttaaa
     6901 cgaattcgtc gagggaccta ataacttcgt atagcataca ttatacgaag ttatacatgt
     6961 ttaagggttc cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct
     7021 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7081 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7141 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7201 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7261 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7321 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7381 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     7441 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7501 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7561 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgatc
     7621 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7681 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7741 ctttaagacc aatgacttac aaggcagctg acgcgtgatt ttaaaagaaa aggggggact
     7801 ggaagggcta attcactccc actgcttttt gcctgtactg ggtctctctg gttagaccag
     7861 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7921 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7981 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag
     8041 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8101 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8521 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8581 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8641 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8701 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9061 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9121 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9181 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9241 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9301 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9361 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9421 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9481 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9541 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9601 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9661 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9721 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9781 aaaaataaac aaataggggt tccgcgcaca tttccccgaa
//
