LOCUS       V001282                 7193 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001282
VERSION     V001282
KEYWORDS    R777-E011 Hs.ARHGAP35
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7193)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 7193)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7193)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..54
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    84..181
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             186..4682
                     /gene="ARHGAP35"
                     /label="Rho GTPase-activating protein 35"
                     /note="Rho GTPase-activating protein 35 from Homo sapiens.
                     Accession#: Q9NRY4"
     protein_bind    complement(4687..4782)
                     /label="attL6"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5160..5948
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      6044..6632
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     6786..6803
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     terminator      complement(6962..6989)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(7081..7167)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgat gatggcaaga aagcaagatg tccgaattcc cacctacaac atcagtgtgg
      241 tgggattatc tgggaccgag aaggaaaagg gccagtgtgg gattggaaag tcttgtttgt
      301 gcaaccgctt cgtgcgcccg agtgctgacg agtttcactt ggaccatacc tccgtcctca
      361 gcaccagtga ctttggaggg cgagtggtca ataatgacca ctttctctac tggggagaag
      421 ttagccgctc cctggaggat tgtgtggaat gtaagatgca cattgtggag cagactgaat
      481 ttattgatga tcagactttt caacctcatc gaagcacggc cctgcagccc tatatcaaga
      541 gagctgctgc gaccaagctt gcatcagctg aaaaactcat gtacttttgc actgaccagc
      601 tggggctgga gcaggacttt gagcagaaac aaatgccaga cggaaagctg ctggttgatg
      661 gttttcttct tggtattgat gttagcaggg gcatgaatag gaactttgat gaccagctca
      721 agtttgtctc caatctctac aatcagcttg caaaaacaaa aaagcccata gtggtggtcc
      781 tgactaagtg tgacgaaggt gttgagcggt acattagaga tgcacatact tttgccttaa
      841 gcaaaaagaa cctccaggtt gtggagacct cagcgagatc caatgtaaac gtggacttgg
      901 ctttcagcac cttagtgcaa ctcattgata aaagtcgggg aaagacaaaa atcattcctt
      961 attttgaagc tctcaagcag cagagtcagc agatagctac agcaaaagac aagtatgagt
     1021 ggctggtgag tcgcattgtg aaaaaccaca atgagaactg gctgagtgtc agccgaaaga
     1081 tgcaggcctc tccagaatac caggactatg tctacctgga agggactcag aaagccaaga
     1141 agctgtttct acagcacatc caccgcctca agcatgagca tatcgagcgt aggagaaagc
     1201 tgtacctggc agccctgcca ttagcttttg aagctcttat acctaatcta gatgaaatag
     1261 accacctaag ctgcataaaa gccaaaaagc tcttagaaac caagccagaa ttcttgaagt
     1321 ggtttgttgt gcttgaagag accccatggg atgccaccag tcacattgac aacatggaaa
     1381 acgaacggat tccctttgat ttaatggata ccgtccctgc agagcagcta tacgaggccc
     1441 acttagagaa gctgaggaac gaaaggaaaa gagttgagat gcgaagggcg tttaaagaaa
     1501 acctggagac ttctcctttc ataactcccg gaaagccttg ggaagaggcc cgtagtttta
     1561 ttatgaatga ggatttctac cagtggctgg aggaatctgt atacatggat atttatggca
     1621 aacaccaaaa gcaaattata gataaagcaa aggaagaatt tcaggagttg cttttggaat
     1681 attcagaatt gttttatgaa ctggagctgg atgctaagcc cagcaaggag aagatgggtg
     1741 ttattcagga tgttctggga gaggaacagc gatttaaagc attacaaaag ctccaagcag
     1801 agcgtgatgc ccttattctg aaacacattc attttgtgta ccacccaaca aaggagacat
     1861 gccccagctg cccagcttgt gtggacgcta agattgagca cttgattagt tctcggttta
     1921 tccggccgtc tgaccggaat cagaaaaatt cactctctga ccctaacatt gatagaatca
     1981 acttggttat attgggcaaa gacggccttg cccgagagtt ggccaatgag attcgagctc
     2041 tttgtacaaa tgatgacaag tatgtgatag atggtaaaat gtatgagctt tccctgaggc
     2101 caatagaggg gaatgtcagg cttcctgtga actctttcca gacgccaaca tttcagcccc
     2161 acggctgtct ctgcctttac aattcaaagg aatcgctatc ctatgtagtg gaaagtatag
     2221 agaagagtag agagtccacg ctgggccggc gggataatca tttagtccat ctccccctta
     2281 cattaatttt ggttaacaag agaggagaca ccagtggaga gactctgcat agcttaatac
     2341 agcaaggtca acaaattgct agcaaacttc agtgtgtctt tctcgaccct gcttctgctg
     2401 gcattggtta cggacgcaac attaatgaaa agcaaatcag tcaagttttg aagggactcc
     2461 tggactctaa gcgtaactta aacctggtca gttctactgc tagcatcaaa gatttggctg
     2521 atgttgatct gcgaattgtt atgtgtctga tgtgtggaga tccttttagt gcagatgaca
     2581 tactttttcc tgtccttcag tcccaaacct gtaaatcttc ccattgtgga agcaacaact
     2641 ctgttttact tgaactacca atcggactgc acaagaagcg gattgaactg tctgttcttt
     2701 cataccattc ctcctttagc atcagaaaga gccggttggt tcatgggtac attgtttttt
     2761 attcagccaa acgtaaggcc tctttggcta tgttacgtgc ctttctttgt gaagtgcagg
     2821 atattatccc tattcagctt gtagcactca ctgatggcgc tgtagatgtc ctggacaatg
     2881 acttaagtag ggaacagcta actgaggggg aggagattgc tcaagaaatt gacggaaggt
     2941 tcacaagcat cccctgtagc caaccccagc ataaacttga gatctttcac ccatttttta
     3001 aagatgtggt ggaaaaaaag aacataatcg aggctactca tatgtacgat aatgctgccg
     3061 aggcctgtag caccaccgaa gaggtgttta actccccccg ggcaggatca ccgctctgca
     3121 actcaaacct gcaggattca gaagaagata tcgagccatc ttacagcctg tttcgagaag
     3181 acacatcact gccttctctg tccaaagacc attctaagct ctctatggaa ctggagggaa
     3241 atgatgggct gtctttcatt atgagcaatt ttgagagtaa actgaacaac aaagtacctc
     3301 cgccagtcaa accaaagcct cctgtccatt ttgaaattac aaagggggat ctatcttatt
     3361 tagaccaagg ccatagggat ggacagagga agtctgtgtc ttctagcccc tggctgcctc
     3421 aggatgggtt tgatccttct gactatgctg aacccatgga tgctgtggtg aagccaagga
     3481 atgaagaaga aaacatatac tccgtgcccc atgacagcac ccaaggcaaa atcatcacca
     3541 ttcggaatat caacaaagcc cagtccaacg gcagcgggaa tggttctgac agtgaaatgg
     3601 acaccagctc tctagagcga gggcgcaagg tttccatcgt gagcaagcca gtgctgtaca
     3661 ggacgagatg cacccggctg gggcggtttg ctagttaccg gaccagcttc agcgtgggga
     3721 gtgatgatga gctggggccc atccggaaga aagaggagga tcaggcatcc cagggttata
     3781 aaggggacaa tgctgtcatt ccatacgaaa cagacgaaga cccgcggagg aggaatattc
     3841 ttcgcagcct aaggaggaac actaagaaac caaagcccaa accccggcca tccatcacaa
     3901 aggcaacctg ggagagtaac tattttgggg tgcccttaac aactgtcgtg actccagaga
     3961 agccgatccc catttttatt gaaagatgta ttgagtacat tgaagccaca ggactgagca
     4021 cggaaggcat ctaccgggtc agcgggaaca agtctgagat ggagagtctg cagagacagt
     4081 ttgatcaaga ccacaacctg gacctggcag agaaagactt tacggtgaat accgtggctg
     4141 gtgccatgaa gagctttttc tcagaactgc ctgaccccct ggtcccgtat aacatgcaga
     4201 tcgacttggt ggaagcacac aaaatcaacg accgggagca gaagttgcat gcccttaagg
     4261 aggtattaaa gaaatttcca aaggaaaacc acgaagtctt caagtatgtc atctctcacc
     4321 taaacaaggt cagccacaac aacaaggtga atctcatgac cagcgagaac ctctccatct
     4381 gcttctggcc caccttgatg agacctgatt tcagcactat ggacgccctc acagccacgc
     4441 gcacctacca gacaatcatt gaactcttta tccagcagtg ccccttcttc ttctacaatc
     4501 ggcccatcac cgagcccccc ggcgccaggc ccagctcccc ctctgccgtg gcttccaccg
     4561 tccccttcct cacttccacg cctgtcacaa gtcagccgtc gcccccacag tcgcctccac
     4621 ccacccccca gtccccaatg cagccactgc ttccctccca gcttcaagcc gaacacacgc
     4681 tgtaatcaac tttcttgtac aaagttggca ttataggaaa gcattgctta tcaatttgtt
     4741 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgcatgcaa gcttagatcc
     4801 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     4861 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     4921 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     4981 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     5041 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     5101 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     5161 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     5221 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     5281 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     5341 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     5401 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     5461 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     5521 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     5581 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     5641 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     5701 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     5761 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     5821 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     5881 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     5941 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     6001 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6061 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6121 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6181 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6241 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6301 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6361 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6421 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6481 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6541 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6601 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6661 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6721 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6781 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6841 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6901 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     6961 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     7021 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     7081 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     7141 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcg
//
