LOCUS       40924_25546        3333 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             379..390
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     promoter        complement(452..470)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        816..920
                     /label=AmpR promoter
     CDS             921..1778
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1952..2540
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2798..2816
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3210..3238
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3246..3262
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag
       61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca
      121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg
      181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc
      241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag
      301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag
      361 atcggggatc tcgagctcat cgaaggtcgc gaaaagcttc agctgggatc cggtaccgat
      421 atcagatctc ccggggcggc cgcgatctgg ttctatagtg tcacctaaat cgtatgtgta
      481 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tccatatggt
      541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
      601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
      661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
      721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2341 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2761 cgattcatta atgcaggtta acctggctta tcgaaattaa tacgactcac tatagggaga
     2821 ccggcctcga gcagcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     2881 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     2941 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     3001 atgcgtccgg cgtagaggat cgatccgggc ttatcgactg cacggtgcac caatgcttct
     3061 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa
     3121 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
     3181 ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg
     3241 tggaattgtg agcggataac aatttcacac aggaaacaga attcccagct tggctgcaga
     3301 accattccat tcgttgatcc gggagtaact cac
//
