LOCUS       40924_25541        3332 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             379..390
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     promoter        complement(451..469)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        815..919
                     /label=AmpR promoter
     CDS             920..1777
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1951..2539
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2797..2815
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3209..3237
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3245..3261
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag
       61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca
      121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg
      181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc
      241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag
      301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag
      361 atcggggatc tcgagctcat cgaaggtcgc gaaagcttca gctgggatcc ggtaccgata
      421 tcagatctcc cggggcggcc gcgatctggt tctatagtgt cacctaaatc gtatgtgtat
      481 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt ccatatggtg
      541 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      601 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      661 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      721 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      781 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      901 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      961 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1021 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1081 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1141 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1201 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1261 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1321 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1381 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1441 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1501 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1561 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1621 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1681 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1741 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1801 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1861 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2341 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2581 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2641 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2701 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2761 gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact atagggagac
     2821 cggcctcgag cagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     2881 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     2941 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     3001 tgcgtccggc gtagaggatc gatccgggct tatcgactgc acggtgcacc aatgcttctg
     3061 gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat
     3121 tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg
     3181 gttctggcaa atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt
     3241 ggaattgtga gcggataaca atttcacaca ggaaacagaa ttcccagctt ggctgcagaa
     3301 ccattccatt cgttgatccg ggagtaactc ac
//
