LOCUS       62056_12815       11345 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1442..1645
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1750..5670
                     /codon_start=1
                     /label=AsCpf1
                     /note="CRISPR-associated protein from Acidaminococcus sp.
                     BV3L6 (Zetsche et al., 2015)"
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     promoter        5872..6083
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6096..6364
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6420..7085
                     /codon_start=1
                     /label=CopGFP
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
                     /translation="LPAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             7155..7208
                     /codon_start=1
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7209..7805
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    7916..8048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(9372..9402)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9417..9438)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9726..10314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10488..11345)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtattatg cccagtacat
     1381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1621 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     1681 tccacgctgt tttgacctcc atagaagatt ctagagctag cgtttaaact taagcttggt
     1741 accgccacca tgacacagtt cgagggcttt accaacctgt atcaggtgag caagacactg
     1801 cggtttgagc tgatcccaca gggcaagacc ctgaagcaca tccaggagca gggcttcatc
     1861 gaggaggaca aggcccgcaa tgatcactac aaggagctga agcccatcat cgatcggatc
     1921 tacaagacct atgccgacca gtgcctgcag ctggtgcagc tggattggga gaacctgagc
     1981 gccgccatcg actcctatag aaaggagaaa accgaggaga caaggaacgc cctgatcgag
     2041 gagcaggcca catatcgcaa tgccatccac gactacttca tcggccggac agacaacctg
     2101 accgatgcca tcaataagag acacgccgag atctacaagg gcctgttcaa ggccgagctg
     2161 tttaatggca aggtgctgaa gcagctgggc accgtgacca caaccgagca cgagaacgcc
     2221 ctgctgcgga gcttcgacaa gtttacaacc tacttctccg gcttttatga gaacaggaag
     2281 aacgtgttca gcgccgagga tatcagcaca gccatcccac accgcatcgt gcaggacaac
     2341 ttccccaagt ttaaggagaa ttgtcacatc ttcacacgcc tgatcaccgc cgtgcccagc
     2401 ctgcgggagc actttgagaa cgtgaagaag gccatcggca tcttcgtgag cacctccatc
     2461 gaggaggtgt tttccttccc tttttataac cagctgctga cacagaccca gatcgacctg
     2521 tataaccagc tgctgggagg aatctctcgg gaggcaggca ccgagaagat caagggcctg
     2581 aacgaggtgc tgaatctggc catccagaag aatgatgaga cagcccacat catcgcctcc
     2641 ctgccacaca gattcatccc cctgtttaag cagatcctgt ccgataggaa caccctgtct
     2701 ttcatcctgg aggagtttaa gagcgacgag gaagtgatcc agtccttctg caagtacaag
     2761 acactgctga gaaacgagaa cgtgctggag acagccgagg ccctgtttaa cgagctgaac
     2821 agcatcgacc tgacacacat cttcatcagc cacaagaagc tggagacaat cagcagcgcc
     2881 ctgtgcgacc actgggatac actgaggaat gccctgtatg agcggagaat ctccgagctg
     2941 acaggcaaga tcaccaagtc tgccaaggag aaggtgcagc gcagcctgaa gcacgaggat
     3001 atcaacctgc aggagatcat ctctgccgca ggcaaggagc tgagcgaggc cttcaagcag
     3061 aaaaccagcg agatcctgtc ccacgcacac gccgccctgg atcagccact gcctacaacc
     3121 ctgaagaagc aggaggagaa ggagatcctg aagtctcagc tggacagcct gctgggcctg
     3181 taccacctgc tggactggtt tgccgtggat gagtccaacg aggtggaccc cgagttctct
     3241 gcccggctga ccggcatcaa gctggagatg gagccttctc tgagcttcta caacaaggcc
     3301 agaaattatg ccaccaagaa gccctactcc gtggagaagt tcaagctgaa ctttcagatg
     3361 cctacactgg cctctggctg ggacgtgaat aaggagaaga acaatggcgc catcctgttt
     3421 gtgaagaacg gcctgtacta tctgggcatc atgccaaagc agaagggcag gtataaggcc
     3481 ctgagcttcg agcccacaga gaaaaccagc gagggctttg ataagatgta ctatgactac
     3541 ttccctgatg ccgccaagat gatcccaaag tgcagcaccc agctgaaggc cgtgacagcc
     3601 cactttcaga cccacacaac ccccatcctg ctgtccaaca atttcatcga gcctctggag
     3661 atcacaaagg agatctacga cctgaacaat cctgagaagg agccaaagaa gtttcagaca
     3721 gcctacgcca agaaaaccgg cgaccagaag ggctacagag aggccctgtg caagtggatc
     3781 gacttcacaa gggattttct gtccaagtat accaagacaa cctctatcga tctgtctagc
     3841 ctgcggccat cctctcagta taaggacctg ggcgagtact atgccgagct gaatcccctg
     3901 ctgtaccaca tcagcttcca gagaatcgcc gagaaggaga tcatggatgc cgtggagaca
     3961 ggcaagctgt acctgttcca gatctataac aaggactttg ccaagggcca ccacggcaag
     4021 cctaatctgc acacactgta ttggaccggc ctgttttctc cagagaacct ggccaagaca
     4081 agcatcaagc tgaatggcca ggccgagctg ttctaccgcc ctaagtccag gatgaagagg
     4141 atggcacacc ggctgggaga gaagatgctg aacaagaagc tgaaggatca gaaaacccca
     4201 atccccgaca ccctgtacca ggagctgtac gactatgtga atcacagact gtcccacgac
     4261 ctgtctgatg aggccagggc cctgctgccc aacgtgatca ccaaggaggt gtctcacgag
     4321 atcatcaagg ataggcgctt taccagcgac aagttctttt tccacgtgcc tatcacactg
     4381 aactatcagg ccgccaattc cccatctaag ttcaaccaga gggtgaatgc ctacctgaag
     4441 gagcaccccg agacacctat catcggcatc gatcggggcg agagaaacct gatctatatc
     4501 acagtgatcg actccaccgg caagatcctg gagcagcgga gcctgaacac catccagcag
     4561 tttgattacc agaagaagct ggacaacagg gagaaggaga gggtggcagc aaggcaggcc
     4621 tggtctgtgg tgggcacaat caaggatctg aagcagggct atctgagcca ggtcatccac
     4681 gagatcgtgg acctgatgat ccactaccag gccgtggtgg tgctggagaa cctgaatttc
     4741 ggctttaaga gcaagaggac cggcatcgcc gagaaggccg tgtaccagca gttcgagaag
     4801 atgctgatcg ataagctgaa ttgcctggtg ctgaaggact atccagcaga gaaagtggga
     4861 ggcgtgctga acccatacca gctgacagac cagttcacct cctttgccaa gatgggcacc
     4921 cagtctggct tcctgtttta cgtgcctgcc ccatatacat ctaagatcga tcccctgacc
     4981 ggcttcgtgg accccttcgt gtggaaaacc atcaagaatc acgagagccg caagcacttc
     5041 ctggagggct tcgactttct gcactacgac gtgaaaaccg gcgacttcat cctgcacttt
     5101 aagatgaaca gaaatctgtc cttccagagg ggcctgcccg gctttatgcc tgcatgggat
     5161 atcgtgttcg agaagaacga gacacagttt gacgccaagg gcaccccttt catcgccggc
     5221 aagagaatcg tgccagtgat cgagaatcac agattcaccg gcagataccg ggacctgtat
     5281 cctgccaacg agctgatcgc cctgctggag gagaagggca tcgtgttcag ggatggctcc
     5341 aacatcctgc caaagctgct ggagaatgac gattctcacg ccatcgacac catggtggcc
     5401 ctgatccgca gcgtgctgca gatgcggaac tccaatgccg ccacaggcga ggactatatc
     5461 aacagccccg tgcgcgatct gaatggcgtg tgcttcgact cccggtttca gaacccagag
     5521 tggcccatgg acgccgatgc caatggcgcc taccacatcg ccctgaaggg ccagctgctg
     5581 ctgaatcacc tgaaggagag caaggatctg aagctgcaga acggcatctc caatcaggac
     5641 tggctggcct acatccagga gctgcgcaac aaaaggccgg cggccacgaa aaaggccggc
     5701 caggcaaaaa agaaaaaggg atcctaccca tacgatgttc cagattacgc ttatccctac
     5761 gacgtgcctg attatgcata cccatatgat gtccccgact atgcctaaga attcgaattt
     5821 aaatcggatc cgcggccgca aggatctgcg atcgctccgg tgcccgtcag tgggcagagc
     5881 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct
     5941 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     6001 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     6061 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg
     6121 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg
     6181 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac
     6241 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt
     6301 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca
     6361 gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc
     6421 tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc
     6481 tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca
     6541 ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct
     6601 accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg
     6661 gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct
     6721 tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct
     6781 tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc
     6841 acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc
     6901 gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc
     6961 accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc
     7021 acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct
     7081 tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct
     7141 ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
     7201 ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     7261 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7321 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7381 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7441 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7501 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7561 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7621 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7681 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7741 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7801 gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7861 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat cagttaactt
     7921 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7981 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8041 tgtctggaat tgactcaaat gatgtcaatt agtctatcag aagctatctg gtctcccttc
     8101 cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta
     8161 tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc
     8221 taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc
     8281 cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     8341 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     8401 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     8461 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     8521 ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
     8581 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
     8641 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
     8701 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt
     8761 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
     8821 tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt ttttccggct
     8881 cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc
     8941 ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt
     9001 tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa
     9061 cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca
     9121 gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa
     9181 ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga
     9241 ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg
     9301 cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9361 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9421 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9481 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9541 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9601 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9781 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9901 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10141 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    10201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10381 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10441 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10501 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10561 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10621 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10681 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10741 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10801 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10861 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10921 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10981 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11041 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11101 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11161 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11221 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11281 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11341 ctcat
//
