LOCUS       V013819                 9231 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013819
VERSION     V013819
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        629..647
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             667..1341
                     /gene="RTP2"
                     /label="Receptor-transporting protein 2"
                     /note="Receptor-transporting protein 2 from Homo sapiens.
                     Accession#: Q5QGT7"
     misc_feature    1345..1918
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             2846..3562
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        complement(3593..3611)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3637..3861
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3871..3918
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(4397..4415)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4422..4438)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4580..5035
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5061..5165
                     /label="AmpR promoter"
     CDS             5166..6023
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6197..6785
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7073..7094
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7109..7139
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7147..7163
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7171..7187
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7208..7226
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             8070..8870
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    8880..8928
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(8939..8972)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 gccaccatgt gtaccagctt gaccacttgt gagtggaaga aagtcttcta tgagaagatg
      721 gaggtggcaa agccagcgga cagctgggag ctcatcatag accccaacct caagcccagt
      781 gagctggccc ctggctggaa gcagtacctg gagcagcacg cctcaggcag gttccactgc
      841 tcctggtgct ggcacacctg gcagtctgcc catgtggtca tcctcttcca catgttcctg
      901 gaccgcgccc agcgggcggg ctcggtgcgc atgcgcgtct tcaagcagct gtgctatgag
      961 tgcggcacgg cgcggctgga cgagtccagc atgctggagg agaacatcga gggcctggtg
     1021 gacaacctca tcaccagcct gcgcgagcag tgctacgagg aggatggtgg ccagtaccgc
     1081 atccacgtgg ccagccgccc ggacagcggg ccgcatcgtg cagagttctg tgaggcctgc
     1141 caggagggca tcgttcactg gaagcccagc gagaagctgc tggaggagga ggtgaccacc
     1201 tacacctctg aagcctccaa gccgagggcc caggcgggat ccggctacaa cttcttgtct
     1261 cttcgctggt gcctcttctg ggcctctctc tgcctgctcg ttgtttacct gcagttctcc
     1321 ttcctcagtc ctgccttctt ttagcccctc tccctccccc ccccctaacg ttactggccg
     1381 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     1441 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     1501 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     1561 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     1621 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     1681 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     1741 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     1801 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     1861 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc
     1921 caccatggtg tcatggatca tctccaggct ggtggtgctt atatttggca ccctttaccc
     1981 tgcgtattat tcctacaagg ctgtgaaatc aaaggacatt aaggaatatg tcaaatggat
     2041 gatgtactgg attatatttg cacttttcac cacagcagag acattcacag acatcttcct
     2101 ttgttggttt ccattctatt atgaactaaa aatagcattt gtagcctggc tgctgtctcc
     2161 ctacacaaaa ggctccagcc tcctgtacag gaagtttgta catcccacgc tatcttcaaa
     2221 agaaaaggaa atcgatgatt gtctggtcca agcaaaagac cgaagttacg atgcccttgt
     2281 gcacttcggg aagcggggct tgaacgtggc cgccacagcg gctgtgatgg ctgcttccaa
     2341 gggacagggt gccttatcgg agagactgcg gagcttcagc atgcaggacc tcaccaccat
     2401 caggggagac ggcgcccctg ctccctcggg ccccccacca ccggggtctg ggcgggccag
     2461 cggcaaacac ggccagccta agatgtccag gagtgcttct gagagcgcta gcagctcagt
     2521 gtgtacctgc tgctccacct gcaggacctg gaaagtggtt gagggagatg tgaatgaggg
     2581 tggtatgaag gcatgggagc cccaccaggt ccaaaaccca ttggcgtttt ctgacgacga
     2641 ggaggaagac ctgctggatt tcatgtacaa gtataaagca cctcgaagga tggaattgcc
     2701 cctggaggca ccgcctagaa tccttcgatc tcgcttcagg aagaaaagta cctcatcctc
     2761 ggccaccgaa accacggcca cgaacttctc tctgttaaag caagcaggag acgtggaaga
     2821 aaaccccggt cctccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     2881 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2941 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3001 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3061 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3121 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3181 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3241 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3301 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3361 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3421 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     3481 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3541 cggcatggac gagctgtaca agtaaagcgg ccgcgactct agagggccct attctatagt
     3601 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     3661 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3721 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3781 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3841 gggatgcggt gggctctatg gcttgtcgac gaagttccta ttccgaagtt cctattctct
     3901 agaaagtata ggaacttcct cgagttaatt aacgagagca taatattgat atgtgccaaa
     3961 gttgtttctg actgactaat aagtataatt tgtttctatt atgtataggt taagctaatt
     4021 acttatttta taatacaaca tgactgtttt taaagtacaa aataagttta tttttgtaaa
     4081 agagagaatg tttaaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt
     4141 ttaataaata aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt
     4201 aaatataata aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga
     4261 taaaacacat gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat
     4321 ctttctaggg ttaaataata gtttctaatt tttttattat tcagcctgct gtcgtgaata
     4381 ccgagctcca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
     4441 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4501 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4561 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4621 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4681 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4741 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4801 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4861 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4921 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4981 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     5041 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5101 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5161 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5221 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5281 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5341 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5401 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5461 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5521 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5581 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5641 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5701 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5761 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5821 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5881 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5941 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6001 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6061 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6121 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6181 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6241 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6301 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6361 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6421 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6481 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6541 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6601 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6661 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6721 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6781 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6841 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6901 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6961 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7021 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7081 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7141 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7201 agctcgaaat taaccctcac taaagggaac aaaagctggt acctcgcgcg acttggtttg
     7261 ccattcttta gcgcgcgtcg cgtcacacag cttggccaca atgtggtttt tgtcaaacga
     7321 agattctatg acgtgtttaa agtttaggtc gagtaaagcg caaatctttt ttaaccctag
     7381 aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc tctttctaaa
     7441 tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga
     7501 ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc gcgtgagtca
     7561 aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat
     7621 tgttatttca tgttctactt acgtgataac ttattatata tatattttct tgttatagat
     7681 atcgaagttc ctattccgaa gttcctattc ttcaaatagt ataggaactt cgtcgagatc
     7741 gatggcgcgc cataacttcg tatagcatac attatacgaa gttatgaatt tcgagcagtg
     7801 tggttttcaa gaggaagcaa aaagcctctc cacccaggcc tggaatgttt ccacccaatg
     7861 tcgagcagtg tggttttgca agaggaagca aaaagcctct ccacccaggc ctggaatgtt
     7921 tccacccaat gtcgagcaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     7981 gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga cgcgtgtggc
     8041 ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa gatggattgc
     8101 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     8161 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     8221 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     8281 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     8341 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     8401 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     8461 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     8521 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     8581 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc gtcgtgaccc
     8641 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     8701 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     8761 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8821 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaggggatc
     8881 ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg atcaattcat
     8941 aacttcgtat agcatacatt atacgaagtt atggcgcgcc ttaattaagg atccgtcgac
     9001 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     9061 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     9121 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     9181 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt t
//
