LOCUS       V026498                12312 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026498
VERSION     V026498
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     728..744
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     promoter        1442..1645
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             1734..7601
                     /label="SCN10A(NM_006514)"
                     /note="SCN10A(NM_006514)"
                     /gene="SCN10A"
     promoter        7649..7860
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7873..8141
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             8176..8775
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    8883..9004
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10291..10307)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10315..10331
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10339..10369)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10384..10405
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10693..11281)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11452..12312)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtattatg cccagtacat
     1381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1621 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     1681 tccacgctgt tttgacctcc atagaagatt ctagagctag cgaattcgcc accatggaat
     1741 tccccattgg atccctcgaa actaacaact tccgtcgctt tactccggag tcactggtgg
     1801 agatagagaa gcaaattgct gccaagcagg gaacaaagaa agccagagag aagcataggg
     1861 agcagaagga ccaagaagag aagcctcggc cccagctgga cttgaaagcc tgcaaccagc
     1921 tgcccaagtt ctatggtgag ctcccagcag aactgatcgg ggagcccctg gaggatctag
     1981 atccgttcta cagcacacac cggacattta tggtgctgaa caaagggagg accatttccc
     2041 ggtttagtgc cactcgggcc ctgtggctat tcagtccttt caacctgatc agaagaacgg
     2101 ccatcaaagt gtctgtccac tcgtggttca gtttatttat tacggtcact attttggtta
     2161 attgtgtgtg catgacccga actgaccttc cagagaaaat tgaatatgtc ttcactgtca
     2221 tttacacctt tgaagccttg ataaagatac tggcaagagg attttgtcta aatgagttca
     2281 cgtacctgag agatccttgg aactggctgg attttagcgt cattaccctg gcatatgttg
     2341 gcacagcaat agatctccgt gggatctcag gcctgcggac attcagagtt cttagagcat
     2401 taaaaacagt ttctgtgatc ccaggcctga aggtcattgt gggggccctg attcactcag
     2461 tgaagaaact ggctgatgtg accatcctca ccatcttctg cctaagtgtt tttgccttgg
     2521 tggggctgca actcttcaag ggcaacctca aaaataaatg tgtcaagaat gacatggctg
     2581 tcaatgagac aaccaactac tcatctcaca gaaaaccaga tatctacata aataagcgag
     2641 gcacttctga ccccttactg tgtggcaatg gatctgactc aggccactgc cctgatggtt
     2701 atatctgcct taaaacttct gacaacccgg attttaacta caccagcttt gattcctttg
     2761 cttgggcttt cctctcactg ttccgcctca tgacacagga ttcctgggaa cgcctctacc
     2821 agcagaccct gaggacttct gggaaaatct atatgatctt ttttgtgctc gtaatcttcc
     2881 tgggatcttt ctacctggtc aacttgatct tggctgtagt caccatggcg tatgaggagc
     2941 agaaccaggc aaccactgat gaaattgaag caaaggagaa gaagttccag gaggccctcg
     3001 agatgctccg gaaggagcag gaggtgctag cagcactagg gattgacaca acctctctcc
     3061 actcccacaa tggatcacct ttaacctcca aaaatgccag tgagagaagg catagaataa
     3121 agccaagagt gtcagagggc tccacagaag acaacaaatc accccgctct gatccttaca
     3181 accagcgcag gatgtctttt ctaggcctcg cctctggaaa acgccgggct agtcatggca
     3241 gtgtgttcca tttccggtcc cctggccgag atatctcact ccctgaggga gtcacagatg
     3301 atggagtctt tcctggagac cacgaaagcc atcggggctc tctgctgctg ggtgggggtg
     3361 ctggccagca aggccccctc cctagaagcc ctcttcctca acccagcaac cctgactcca
     3421 ggcatggaga agatgaacac caaccgccgc ccactagtga gcttgcccct ggagctgtcg
     3481 atgtctcggc attcgatgca ggacaaaaga agactttctt gtcagcagaa tacttagatg
     3541 aacctttccg ggcccaaagg gcaatgagtg ttgtcagtat cataacctcc gtccttgagg
     3601 aactcgagga gtctgaacag aagtgcccac cctgcttgac cagcttgtct cagaagtatc
     3661 tgatctggga ttgctgcccc atgtgggtga agctcaagac aattctcttt gggcttgtga
     3721 cggatccctt tgcagagctc accatcacct tgtgcatcgt ggtgaacacc atcttcatgg
     3781 ccatggagca ccatggcatg agccctacct tcgaagccat gctccagata ggcaacatcg
     3841 tctttaccat attttttact gctgaaatgg tcttcaaaat cattgccttc gacccatact
     3901 attatttcca gaagaagtgg aatatctttg actgcatcat cgtcactgtg agtctgctag
     3961 agctgggcgt ggccaagaag ggaagcctgt ctgtgctgcg gagcttccgc ttgctgcgcg
     4021 tattcaagct ggccaaatcc tggcccacct taaacacact catcaagatc atcggaaact
     4081 cagtgggggc actggggaac ctcaccatca tcctggccat cattgtcttt gtctttgctc
     4141 tggttggcaa gcagctccta ggggaaaact accgtaacaa ccgaaaaaat atctccgcgc
     4201 cccatgaaga ctggccccgc tggcacatgc acgacttctt ccactctttc ctcattgtct
     4261 tccgtatcct ctgtggagag tggattgaga acatgtgggc ctgcatggaa gttggccaaa
     4321 aatccatatg cctcatcctt ttcttgacgg tgatggtgct agggaacctg gtggtgctta
     4381 acctgttcat cgccctgcta ttgaactctt tcagtgctga caacctcaca gccccggagg
     4441 acgatgggga ggtgaacaac ctgcaggtgg ccctggcacg gatccaggtc tttggccatc
     4501 gtaccaaaca ggctctttgc agcttcttca gcaggtcctg cccattcccc cagcccaagg
     4561 cagagcctga gctggtggtg aaactcccac tctccagctc caaggctgag aaccacattg
     4621 ctgccaacac tgccaggggg agctctggag ggctccaagc tcccagaggc cccagggatg
     4681 agcacagtga cttcatcgct aatccgactg tgtgggtctc tgtgcccatt gctgagggtg
     4741 aatctgatct tgatgacttg gaggatgatg gtggggaaga tgctcagagc ttccagcagg
     4801 aagtgatccc caaaggacag caggagcagc tgcagcaagt cgagaggtgt ggggaccacc
     4861 tgacacccag gagcccaggc actggaacat cttctgagga cctggctcca tccctgggtg
     4921 agacgtggaa agatgagtct gttcctcagg tccctgctga gggagtggac gacacaagct
     4981 cctctgaggg cagcacggtg gactgcctag atcctgagga aatcctgagg aagatccctg
     5041 agctggcaga tgacctggaa gaaccagatg actgcttcac agaaggatgc attcgccact
     5101 gtccctgctg caaactggat accaccaaga gtccatggga tgtgggctgg caggtgcgca
     5161 agacttgcta ccgtatcgtg gagcacagct ggtttgagag cttcatcatc ttcatgatcc
     5221 tgctcagcag tggatctctg gcctttgaag actattacct ggaccagaag cccacggtga
     5281 aagctttgct ggagtacact gacagggtct tcacctttat ctttgtgttc gagatgctgc
     5341 ttaagtgggt ggcctatggc ttcaaaaagt acttcaccaa tgcctggtgc tggctggact
     5401 tcctcattgt gaatatctca ctgataagtc tcacagcgaa gattctggaa tattctgaag
     5461 tggctcccat caaagccctt cgaacccttc gcgctctgcg gccactgcgg gctctttctc
     5521 gatttgaagg catgcgggtg gtggtggatg ccctggtggg cgccatccca tccatcatga
     5581 atgtcctcct cgtctgcctc atcttctggc tcatcttcag catcatgggt gtgaacctct
     5641 tcgcagggaa gttttggagg tgcatcaact ataccgatgg agagttttcc cttgtacctt
     5701 tgtcgattgt gaataacaag tctgactgca agattcaaaa ctccactggc agcttcttct
     5761 gggtcaatgt gaaagtcaac tttgataatg ttgcaatggg ttaccttgca cttctgcagg
     5821 tggcaacctt taaaggctgg atggacatta tgtatgcagc tgttgattcc cgggaggtca
     5881 acatgcaacc caagtgggag gacaacgtgt acatgtattt gtactttgtc atcttcatca
     5941 tttttggagg cttcttcaca ctgaatctct ttgttggggt cataattgac aacttcaatc
     6001 aacagaaaaa aaagttaggg ggccaggaca tcttcatgac agaggagcag aagaaatact
     6061 acaatgccat gaagaagttg ggctccaaga agccccagaa gcccatccca cggcccctga
     6121 acaagttcca gggttttgtc tttgacatcg tgaccagaca agcttttgac atcaccatca
     6181 tggtcctcat ctgcctcaac atgatcacca tgatggtgga gactgatgac caaagtgaag
     6241 aaaagacgaa aattctgggc aaaatcaacc agttctttgt ggccgtcttc acaggcgaat
     6301 gtgtcatgaa gatgttcgct ttgaggcagt actacttcac aaatggctgg aatgtgtttg
     6361 acttcattgt ggtggttctc tccattgcga gcctgatttt ttctgcaatt cttaagtcac
     6421 ttcaaagtta cttctcccca acgctcttca gagtcatccg cctggcccga attggccgca
     6481 tcctcagact gatccgagcg gccaagggga tccgcacact gctctttgcc ctcatgatgt
     6541 ccctgcctgc cctcttcaac atcgggctgt tgctattcct tgtcatgttc atctactcta
     6601 tcttcggtat gtccagcttt ccccatgtga ggtgggaggc tggcatcgac gacatgttca
     6661 acttccagac cttcgccaac agcatgctgt gcctcttcca gattaccacg tcggccggct
     6721 gggatggcct cctcagcccc atcctcaaca cagggccccc ctactgtgac cccaatctgc
     6781 ccaacagcaa tggcaccaga ggggactgtg ggagcccagc cgtaggcatc atcttcttca
     6841 ccacctacat catcatctcc ttcctcatca tggtcaacat gtacattgca gtgattctgg
     6901 agaacttcaa tgtggccacg gaggagagca ctgagcccct gagtgaggac gactttgaca
     6961 tgttctatga gacctgggag aagtttgacc cagaggccac tcagtttatt accttttctg
     7021 ctctctcgga ctttgcagac actctctctg gtcccctgag aatcccaaaa cccaatcgaa
     7081 atatactgat ccagatggac ctgcctttgg tccctggaga taagatccac tgcttggaca
     7141 tcctttttgc tttcaccaag aatgtcctag gagaatccgg ggagttggat tctctgaagg
     7201 caaatatgga ggagaagttt atggcaacta atctttcaaa atcatcctat gaaccaatag
     7261 caaccactct ccgatggaag caagaagaca tttcagccac tgtcattcaa aaggcctatc
     7321 ggagctatgt gctgcaccgc tccatggcac tctctaacac cccatgtgtg cccagagctg
     7381 aggaggaggc tgcatcactc ccagatgaag gttttgttgc attcacagca aatgaaaatt
     7441 gtgtactccc agacaaatct gaaactgctt ctgccacatc attcccaccg tcctatgaga
     7501 gtgtcactag aggccttagt gatagagtca acatgaggac atctagctca atacaaaatg
     7561 aagatgaagc caccagtatg gagctgattg cccctgggcc ctagtaggcg gccgcgaagg
     7621 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     7681 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     7741 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     7801 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     7861 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     7921 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     7981 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     8041 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     8101 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     8161 acgctagacg ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8221 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8281 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8341 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8401 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8461 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8521 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8581 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8641 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8701 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     8761 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8821 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcag
     8881 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8941 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9001 cttatcatgt ctggaattga ctcaaatgat gtcaattagt ctatcagaag ctatctggtc
     9061 tcccttccgg gggacaagac atccctgttt aatatttaaa cagcagtgtt cccaaactgg
     9121 gttcttatat cccttgctct ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg
     9181 aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg attccttttt
     9241 tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag
     9301 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
     9361 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
     9421 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg
     9481 gggctgtccc tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     9541 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     9601 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     9661 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     9721 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     9781 cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt
     9841 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
     9901 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     9961 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
    10021 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
    10081 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
    10141 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
    10201 tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
    10261 tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    10321 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    10381 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10441 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10501 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10561 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10621 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10681 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10741 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10801 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10861 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10921 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10981 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    11041 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    11101 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    11161 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    11221 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    11281 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    11341 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    11401 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    11461 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11521 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11581 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11641 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11701 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11761 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11821 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11881 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11941 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    12001 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    12061 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    12121 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    12181 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    12241 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    12301 ttgaatactc at
//
