LOCUS       V021357                 9918 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021357
VERSION     V021357
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     728..744
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     promoter        1442..1645
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             1734..5210
                     /label="KCNH2(NM_000238)"
                     /note="KCNH2(NM_000238)"
                     /gene="KCNH2"
     promoter        5255..5466
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5479..5747
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5782..6381
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    6489..6610
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7897..7913)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7921..7937
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7945..7975)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7990..8011
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8299..8887)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9058..9918)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtattatg cccagtacat
     1381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1621 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     1681 tccacgctgt tttgacctcc atagaagatt ctagagctag cgaattcgcc accatgccgg
     1741 tgcggagggg ccacgtcgcg ccgcagaaca ccttcctgga caccatcatc cgcaagtttg
     1801 agggccagag ccgtaagttc atcatcgcca acgctcgggt ggagaactgc gccgtcatct
     1861 actgcaacga cggcttctgc gagctgtgcg gctactcgcg ggccgaggtg atgcagcgac
     1921 cctgcacctg cgacttcctg cacgggccgc gcacgcagcg ccgcgctgcc gcgcagatcg
     1981 cgcaggcact gctgggcgcc gaggagcgca aagtggaaat cgccttctac cggaaagatg
     2041 ggagctgctt cctatgtctg gtggatgtgg tgcccgtgaa gaacgaggat ggggctgtca
     2101 tcatgttcat cctcaatttc gaggtggtga tggagaagga catggtgggg tccccggctc
     2161 atgacaccaa ccaccggggc ccccccacca gctggctggc cccaggccgc gccaagacct
     2221 tccgcctgaa gctgcccgcg ctgctggcgc tgacggcccg ggagtcgtcg gtgcggtcgg
     2281 gcggcgcggg cggcgcgggc gccccggggg ccgtggtggt ggacgtggac ctgacgcccg
     2341 cggcacccag cagcgagtcg ctggccctgg acgaagtgac agccatggac aaccacgtgg
     2401 cagggctcgg gcccgcggag gagcggcgtg cgctggtggg tcccggctct ccgccccgca
     2461 gcgcgcccgg ccagctccca tcgccccggg cgcacagcct caaccccgac gcctcgggct
     2521 ccagctgcag cctggcccgg acgcgctccc gagaaagctg cgccagcgtg cgccgcgcct
     2581 cgtcggccga cgacatcgag gccatgcgcg ccggggtgct gcccccgcca ccgcgccacg
     2641 ccagcaccgg ggccatgcac ccactgcgca gcggcttgct caactccacc tcggactccg
     2701 acctcgtgcg ctaccgcacc attagcaaga ttccccaaat caccctcaac tttgtggacc
     2761 tcaagggcga ccccttcttg gcttcgccca ccagtgaccg tgagatcata gcacctaaga
     2821 taaaggagcg aacccacaat gtcactgaga aggtcaccca ggtcctgtcc ctgggcgccg
     2881 acgtgctgcc tgagtacaag ctgcaggcac cgcgcatcca ccgctggacc atcctgcatt
     2941 acagcccctt caaggccgtg tgggactggc tcatcctgct gctggtcatc tacacggctg
     3001 tcttcacacc ctactcggct gccttcctgc tgaaggagac ggaagaaggc ccgcctgcta
     3061 ccgagtgtgg ctacgcctgc cagccgctgg ctgtggtgga cctcatcgtg gacatcatgt
     3121 tcattgtgga catcctcatc aacttccgca ccacctacgt caatgccaac gaggaggtgg
     3181 tcagccaccc cggccgcatc gccgtccact acttcaaggg ctggttcctc atcgacatgg
     3241 tggccgccat ccccttcgac ctgctcatct tcggctctgg ctctgaggag ctgatcgggc
     3301 tgctgaagac tgcgcggctg ctgcggctgg tgcgcgtggc gcggaagctg gatcgctact
     3361 cagagtacgg cgcggccgtg ctgttcttgc tcatgtgcac ctttgcgctc atcgcgcact
     3421 ggctagcctg catctggtac gccatcggca acatggagca gccacacatg gactcacgca
     3481 tcggctggct gcacaacctg ggcgaccaga taggcaaacc ctacaacagc agcggcctgg
     3541 gcggcccctc catcaaggac aagtatgtga cggcgctcta cttcaccttc agcagcctca
     3601 ccagtgtggg cttcggcaac gtctctccca acaccaactc agagaagatc ttctccatct
     3661 gcgtcatgct cattggctcc ctcatgtatg ctagcatctt cggcaacgtg tcggccatca
     3721 tccagcggct gtactcgggc acagcccgct accacacaca gatgctgcgg gtgcgggagt
     3781 tcatccgctt ccaccagatc cccaatcccc tgcgccagcg cctcgaggag tacttccagc
     3841 acgcctggtc ctacaccaac ggcatcgaca tgaacgcggt gctgaagggc ttccctgagt
     3901 gcctgcaggc tgacatctgc ctgcacctga accgctcact gctgcagcac tgcaaaccct
     3961 tccgaggggc caccaagggc tgccttcggg ccctggccat gaagttcaag accacacatg
     4021 caccgccagg ggacacactg gtgcatgctg gggacctgct caccgccctg tacttcatct
     4081 cccggggctc catcgagatc ctgcggggcg acgtcgtcgt ggccatcctg gggaagaatg
     4141 acatctttgg ggagcctctg aacctgtatg caaggcctgg caagtcgaac ggggatgtgc
     4201 gggccctcac ctactgtgac ctacacaaga tccatcggga cgacctgctg gaggtgctgg
     4261 acatgtaccc tgagttctcc gaccacttct ggtccagcct ggagatcacc ttcaacctgc
     4321 gagataccaa catgatcccg ggctcccccg gcagtacgga gttagagggt ggcttcagtc
     4381 ggcaacgcaa gcgcaagttg tccttccgca ggcgcacgga caaggacacg gagcagccag
     4441 gggaggtgtc ggccttgggg ccgggccggg cgggggcagg gccgagtagc cggggccggc
     4501 cgggggggcc gtggggggag agcccgtcca gtggcccctc cagccctgag agcagtgagg
     4561 atgagggccc aggccgcagc tccagccccc tccgcctggt gcccttctcc agccccaggc
     4621 cccccggaga gccgccgggt ggggagcccc tgatggagga ctgcgagaag agcagcgaca
     4681 cttgcaaccc cctgtcaggc gccttctcag gagtgtccaa cattttcagc ttctgggggg
     4741 acagtcgggg ccgccagtac caggagctcc ctcgatgccc cgcccccacc cccagcctcc
     4801 tcaacatccc cctctccagc ccgggtcggc ggccccgggg cgacgtggag agcaggctgg
     4861 atgccctcca gcgccagctc aacaggctgg agacccggct gagtgcagac atggccactg
     4921 tcctgcagct gctacagagg cagatgacgc tggtcccgcc cgcctacagt gctgtgacca
     4981 ccccggggcc tggccccact tccacatccc cgctgttgcc cgtcagcccc ctccccaccc
     5041 tcaccttgga ctcgctttct caggtttccc agttcatggc gtgtgaggag ctgcccccgg
     5101 gggccccaga gcttccccaa gaaggcccca cacgacgcct ctccctaccg ggccagctgg
     5161 gggccctcac ctcccagccc ctgcacagac acggctcgga cccgggcagt taggcggccg
     5221 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5281 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     5341 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5401 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5461 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     5521 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     5581 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5641 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5701 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5761 gcgcctacgc tagacgccac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5821 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5881 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5941 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6001 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6061 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6121 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6181 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6241 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6301 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6361 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     6421 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6481 tatcagttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6541 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6601 atgtatctta tcatgtctgg aattgactca aatgatgtca attagtctat cagaagctat
     6661 ctggtctccc ttccggggga caagacatcc ctgtttaata tttaaacagc agtgttccca
     6721 aactgggttc ttatatccct tgctctggtc aaccaggttg cagggtttcc tgtcctcaca
     6781 ggaacgaagt ccctaaagaa acagtggcag ccaggtttag ccccggaatt gactggattc
     6841 cttttttagg gcccattggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc
     6901 aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg
     6961 ctgtccccgc acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc
     7021 cgggcggctc gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt
     7081 gggggggggc tgtccctgat atctataaca agaaaatata tatataataa gttatcacgt
     7141 aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa
     7201 aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt
     7261 accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc
     7321 acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca
     7381 attttacgca gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt
     7441 cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag
     7501 cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct
     7561 gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca
     7621 tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc
     7681 ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg
     7741 gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat
     7801 attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc
     7861 gtgagttacc cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7921 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7981 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8041 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8101 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8701 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9901 gaaatgttga atactcat
//
