LOCUS       62056_22800        8280 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(41..57)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    260..284
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1584..2210
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2642..3712
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3781..3975
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4319..4459
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4645..5233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5323..6114)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6539..6563
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6641..6815)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        6870..7224
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             7229..8251
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 gggatcctct agagtcgacc tgcaggcatg caagcttggc actggccgtc gttttacaac
       61 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      181 gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc
      241 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
      301 tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
      361 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
      421 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
      481 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
      541 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
      601 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
      661 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
      721 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
      781 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
      841 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
      901 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
      961 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     1021 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     1081 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     1141 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     1201 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     1261 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     1321 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     1381 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     1441 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     1501 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     1561 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     1621 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     1681 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     1741 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     1801 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     1861 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     1921 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     1981 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     2041 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     2101 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     2161 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     2221 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     2281 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     2341 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     2401 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     2461 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     2521 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     2581 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     2641 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     2701 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     2761 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     2821 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     2881 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     2941 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     3001 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     3061 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     3121 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     3181 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     3241 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     3301 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     3361 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     3421 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     3481 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     3541 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     3601 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     3661 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     3721 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     3781 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     3841 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     3901 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     3961 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     4021 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     4081 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     4141 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     4201 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4261 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     4321 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     4381 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     4441 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4501 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4561 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5281 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
     5341 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     5401 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
     5461 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
     5521 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
     5581 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
     5641 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
     5701 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
     5761 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
     5821 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
     5881 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
     5941 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
     6001 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
     6061 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
     6121 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     6181 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     6241 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
     6301 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     6361 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     6421 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     6481 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     6541 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
     6601 cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg
     6661 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
     6721 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
     6781 tatctgggaa ctactcacac attattatgg agaaactcga gggtgggctc gcagatcttc
     6841 actagtggga attcgagctc ggtacccaac tgatattgaa ggagcatttt ttgggcttgg
     6901 ctggagctag tggaggtcaa caatgaatgc ctattttggt ttagtcgtcc aggcggtgag
     6961 cacaaaattt gtgtcgtttg acaagatggt tcatttaggc aactggtcag atcagcccca
     7021 cttgtagcag tagcggcggc gctcgaagtg tgactcttat tagcagacag gaacgaggac
     7081 attattatca tctgctgctt ggtgcacgat aacttggtgc gtttgtcaag caaggtaagt
     7141 ggacgacccg gtcatacctt cttaagttcg cccttcctcc ctttatttca gattcaatct
     7201 gacttaccta ttctacccaa gcatccaaat gaaaaagcct gaactcaccg cgacgtctgt
     7261 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     7321 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     7381 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     7441 gctcccgatt ccggaagtgc ttgacattgg ggagttcagc gagagcctga cctattgcat
     7501 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     7561 tctccagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     7621 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     7681 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     7741 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     7801 ccggcacctc gtgcatgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     7861 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     7921 catcctcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     7981 gaggcatccg gagcttgcag gatcgccgcg cctccgggcg tatatgctcc gcattggtct
     8041 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     8101 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     8161 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     8221 acgccccagc actcgtccga gggcaaagga atagagtaga tgccgaccgg gaaccagttg
//
