LOCUS       PX551.        7400 bp DNA     circular SYN 11-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    PX551.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        160..388
                     /label=Mecp2 promoter
                     /note="promoter for selective expression in neurons (Adachi
                     et al., 2005; Gray et al., 2011)"
     CDS             404..430
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             434..454
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=nuclear localization signal of SV40 large T
                     ant
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             464..4564
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             4568..4588
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=nuclear localization signal of SV40 large T
                     ant
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     polyA_signal    4607..4655
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     repeat_region   4663..4803
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4878..5333
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5615..5719
                     /label=AmpR promoter
     CDS             5720..6577
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6751..7339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggcctct agaaagctta gctgaatggg gtccgcctct
      181 tttccctgcc taaacagaca ggaactcctg ccaattgagg gcgtcaccgc taaggctccg
      241 ccccagcctg ggctccacaa ccaatgaagg gtaatctcga caaagagcaa ggggtggggc
      301 gcgggcgcgc aggtgcagca gcacacaggc tggtcgggag ggcggggcgc gacgtctgcc
      361 gtgcggggtc ccggcatcgg ttgcgcgcac cggtgccacc atgtacccat acgatgttcc
      421 agattacgct tcgccgaaga aaaagcgcaa ggtcgaagcg tccgacaaga agtacagcat
      481 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      541 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      601 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
      661 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      721 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      781 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
      841 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
      901 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
      961 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
     1021 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
     1081 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc
     1141 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg
     1201 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
     1261 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
     1321 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
     1381 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
     1441 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
     1501 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc
     1561 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
     1621 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa
     1681 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
     1741 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
     1801 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
     1861 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc
     1921 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
     1981 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt
     2041 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc
     2101 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
     2161 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
     2221 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
     2281 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
     2341 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
     2401 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
     2461 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
     2521 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
     2581 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
     2641 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
     2701 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
     2761 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
     2821 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
     2881 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
     2941 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct
     3001 gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag
     3061 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct
     3121 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg
     3181 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
     3241 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga
     3301 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
     3361 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
     3421 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
     3481 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
     3541 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
     3601 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
     3661 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct
     3721 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
     3781 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga
     3841 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
     3901 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
     3961 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
     4021 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
     4081 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
     4141 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
     4201 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga
     4261 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
     4321 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga
     4381 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
     4441 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
     4501 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
     4561 cgacagcccc aagaagaaga gaaaggtgga ggccagctaa gaattcaata aaagatcttt
     4621 attttcatta gatctgtgtg ttggtttttt gtgtgcggcc gcaggaaccc ctagtgatgg
     4681 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4741 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     4801 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4861 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4921 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4981 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5041 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     5101 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5161 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     5221 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5281 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     5341 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5401 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5461 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5521 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5581 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5641 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5701 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5761 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5821 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5881 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5941 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6001 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6061 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6121 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6181 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6241 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6301 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6361 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6421 agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6481 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6541 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6601 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6661 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6721 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6781 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6841 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6901 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6961 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7021 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7081 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7141 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7201 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7261 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7321 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7381 ctggcctttt gctcacatgt
//
