LOCUS 40924_40682 9093 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector PSM-123456_Gli3-DT, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9093) AUTHORS Vega Lopez GA, Cerrizuela S, Aguero TH, Palacio MB, Tribulo C, Aybar MJ. TITLE The gli3 transcriptional activator is a key effector of Indian hedgehog signaling during Xenopus neural crest development JOURNAL Unpublished REFERENCE 2 (bases 1 to 9093) AUTHORS Vega Lopez GA, Cerrizuela S, Aguero TH, Palacio MB, Tribulo C, Aybar MJ. TITLE Direct Submission JOURNAL Submitted (03-JAN-2017) Developmental Biology, Instituto Superior de Investigaciones Biologicas (INSIBIO, CONICET-UNT), Chacabuco 461, Tucuman, Tucuman T4000ILI, Argentina REFERENCE 3 (bases 1 to 9093) TITLE Direct Submission REFERENCE 4 (bases 1 to 9093) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (03-JAN-2017) Developmental Biology, Instituto Superior de Investigaciones Biologicas (INSIBIO, CONICET-UNT), Chacabuco 461, Tucuman, Tucuman T4000ILI, Argentina" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Assembly Method :: vector nti v. 11 Sequencing Technology :: Applied Biosystems 3130 Genetic Analyzer ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..9093 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 35..53 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" CDS 103..132 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 142..171 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 181..210 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 220..249 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 259..288 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 322..351 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 379..5085 /gene="gli3" /label=gli3 /note="Zinc finger protein GLI3 from Xenopus laevis. Accession#: Q91660" CDS 5092..5109 /label=6xHis /note="6xHis affinity tag" promoter complement(5120..5137) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal complement(5142..5276) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(5389..5407) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(5428..5444) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(5452..5468) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5476..5506) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(5521..5542) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5830..6418) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6592..7449) /label=AmpR /note="beta-lactamase" promoter complement(7450..7554) /label=AmpR promoter rep_origin complement(7580..8035) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 8109..9093 /label=CMV IE94 promoter /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" ORIGIN 1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag 61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggagcaaaa gctcatttct 121 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg 181 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa 241 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag 301 agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt gaattcaagg 361 cctctcgagc ctctagacgt ggaagcccag tcccgtagca ctacagcaag tgaaaagaaa 421 aaggttgaga actctatagt aaaaggtcac tctcgaacag aagtcagtga gaaagctgtt 481 gcctcaagca caacttccaa tgaggatgaa agtcctggac agacatatca cagagaaaga 541 agaaacgcca ttgccatgca gcctcaagga ggccaaggtc ttggtaaaat cagcgaagag 601 ccctctacat caagtgagga aagggcctca ttaataaaaa aggaactcca tggatctata 661 acacatcttc ctgaaccctc agttccctac cggggcactg tctttgccat ggatcctagg 721 aatggttata tagatcctca ttaccgatcc gacctcgatc ttttcccggc ctttcatcct 781 cctgtgccaa ttgatgcaag acatcatgag ggaagatacc attatgaacc aactcccatt 841 cctccgctgc atgtgcctac tgcattagca agcagcccaa catacccaga tctcccattc 901 ttccggatat ctcctcatag aaatccagcc agtgcatctg attccccatt cagccctcct 961 catccgtaca tcagtcctta catggactac atccgatctc tccacagcag cccctcactg 1021 tctatgatat cggctgctcg tggactcagc cctaccgatg tcccacatgc tggcattagc 1081 cctgctgaat attaccacca aatggcactg ctggctggac agcgaagccc atatgctgat 1141 atttttccat cccctgcaac tgctggtgct ggagctaatg cccttcatat ggaatatctt 1201 catgccatgg agagctcacg gttttccagt cccagactat cagcacgacc aagtcgcaag 1261 agaacgctgt ctatatcacc cctttctgat catagctttg atctccagac aatgattcgc 1321 aactctccaa attctctcgt tactatcctt aacaactccc gcagcagctc atctgctagt 1381 ggctcttatg ggcacctagc agccagtgcc atcagtccag ctttgaattt tgcataccag 1441 cctaccccag tgtctcttca gcaaatgcat caacagatta tgagtcgaca gcacagcata 1501 ggctcggcct ttggacacag ccctccactt cttcacccag caccaacatt tccttctcaa 1561 agaaccattc ctggtatccc cagtgttttg aatccagtgc aggtcagcat tggcccatca 1621 gaggctgcac agcagaacaa gcccaccagt gagtcagcag tcagcagcac tggggaccta 1681 ctgcacaaca agcgctctaa ggtaaagcct gaagaagatc atccaagccc tggagcagtg 1741 tgcattcagg atcagccaga tggaatgact ctggtgaaag aggagggggt caaagatgaa 1801 agtaaacaag aggctgaagt ggtctatgag acaaactgcc attgggaagg ctgctcgcga 1861 gagtttgaca cacaggaaca actagtgcat catatcaaca atgaccacat acatggagag 1921 aaaaaggagt ttgtgtgtcg atggctggat tgttcaagag agcaaaagcc gttcaaggct 1981 cagtatatgc tggtggtcca catgagaaga catacaggag aaaagccaca caaatgcact 2041 tttgaaggtt gttcaaaagc ctactccaga ctagaaaact tgaaaactca cctgagatct 2101 cacactgggg agaaaccata tgtctgtgag catgagggct gcaacaaagc cttttcaaat 2161 gcatctgaca gagccaagca ccagaaccgc actcattcaa acgagaaacc ttatgtttgc 2221 aagatcccag ggtgtacaaa acgttacact gaccctagct cactcagaaa gcatgtaaag 2281 acagtgcatg gtccagaggc ccatgtgacc aagaagcaga gaggggacat acacccaagg 2341 cctccaccac caagagaacc aggaagccat tcacagtcga gatcaccagg ccaacagact 2401 cagggtatac atggagagca caaggacctc agtaacacta cctcaaagca tgaagaatgc 2461 ctgcaagtaa gatcagtgaa gactgaaaaa ccaatgtcat ctcagccaag ccctggtggt 2521 aagtcttcat gcagcaggca gcagtccccc atcagcaact ataccaacag tgggatcgag 2581 cttaatctaa acagtggagg cagcattgga gacttcagtg ccctagatga aaccccaatc 2641 atggactcta ccatttccac cgcaaccaca ggacttggtt tacacggaag gagggttatg 2701 acaggaacca aatggatgga gcaaataaaa gttgaaaaaa tgaaacaagt caatgggatg 2761 cttccaagac tgaacccagt tccacctcat agagcaccaa ctttaccacc aatcactgga 2821 aatggcgtgc agctgaacag caacatcagt ttgggaggat tagcacctgt acttccaaac 2881 agaaatgacc tttctagcat ggacattaca gtcttgaata tgcttaacag aagagatgcc 2941 aataccagca caataagttc agcctatatg agcagccgca ggtcagctgg aatttcacct 3001 tgtttttcca gtcgaggatc cgctgaggca tctcagtttg agggacgggt tcagaattta 3061 agtgttgcag gctcctatga tccaatttct actgatgcct cgagacgttc agctgaaaca 3121 agccagtgtg atgggctgcc aaccctcctt agcctaactc cagcacagca atacaggctg 3181 aaagctaaat atgctgctgc gacaggtggg cccccaccaa caccactgcc taatatggaa 3241 agaatgagcc taaaaaccag aatggctctc ttgggtgact caagagattg tagattatca 3301 cctcttcctt cggtcaatgt cccacggaga tgtgctgatg gtggagtagg ctcttatgga 3361 agaaggcatt tgttgccaaa tgaacttcaa gttaatggaa ttcgtagagc agcggatcct 3421 gtaaaaatgg tctctgataa tgtttctaat actagagttg taaggttcaa cagtctaaac 3481 aacgttattc ctccagatgt cccaccacct atggaaaggc gcaatttaag tcttcaaaac 3541 tatacccgca atgtgtattc accatgtcct cctagcataa gtgaaaatgt tgccctggaa 3601 gcgatgatta tggaagcaga aggcaatttt gatgacgagg atttactgcc agatgacatg 3661 gttcagtatc ttaactctcg gaaccaaaga ctttatgaaa ccattgaaac agatgcatta 3721 cagaataatg ctggagaaag aaatggaaac aactttgaac aatcccatgt gaccagtaat 3781 gtaataaatg aacagttcca cagtagtgaa caaacagatc tcatagaaaa taaaaatgac 3841 ctcccaattc agtggaatga agtaagctct ggcagtgcag aatattctcc atcaagattg 3901 aagtatgggc agcgctttcc cacacagcag aatcaacctt ttggattata taataacatg 3961 atggtccaac agcaaaatgt accaaagagc gggttgtccc agcaaagggg ctatcaacat 4021 catacacaaa acaacccaca agccccccag caaaatttgg atctttacaa taacagcaat 4081 gtgtggagtg gtcagttagg caggggcaat cagtacattg atgacataga aagatcatca 4141 ctgggacatt ctgcaacagg aagtctttgc aattcagcag ctcgaggaca aaaacttaca 4201 accaatggtt tgccaatgaa tactggtcaa caaaactttg gcccatccac acattatagt 4261 gtgcaatcag ctaatagagc tgaagtagtc cagaatgaaa acatcataaa tcaggaattc 4321 atgcaacaaa tgacagctga acacctgatc tatggtatgc atttgctggg ggtgactcga 4381 tctaatcaga caacatcagg gcaaaatgga aacacaactg acggaactcg aagtttcctg 4441 tcaactactc aaaatggtgg cgagcagcag ccaacccttg caaagaattt ccagtctttt 4501 gtaaactcta gtggaaatag caggcagaat ttacacagaa acaacctgcc atatctgcaa 4561 gagcaaatat atgacactaa ccaacacatt ttcaaagtga acagtattaa gatggaaatg 4621 cagagtcacc cacaacagca ctgtgcaaac gcacaaaact tctccggtca gttgtatgac 4681 caaacagcaa cataccctca acaaattatg aaatcaggct ctgaacccgg tatggaagca 4741 aactgtcttt tacaagaaag taacaataca aactcatcca agcttctttc acctggggct 4801 aaccaagtga ctagcacagt cgataacatt gataacagtt tggatggggt acaaattgat 4861 tttgatgcta tcattgatga tggtgatcat gccagcctca tttctggagt tctaagtcca 4921 agtatcatcc agaatctctc ccgtaattct tcacgcctta ccactcctcg agcatctctg 4981 acattcccag ccatacctgt tagtacatct aacatggcta ttggggacat gagttctttg 5041 ctgacctcac ttgcagaaga aagcaagttt cttgctatta tgcaactgca gcatcatcac 5101 catcaccatt aatctagaac tatagtgagt cgtattacgt agatccagac atgataagat 5161 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 5221 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 5281 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat 5341 tcgcggccgc ggcgccaatg cattgggccc ggtacccagc ttttgttccc tttagtgagg 5401 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 5461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 5521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 5581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 5641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 5701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 5761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 5821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 5881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 5941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6241 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 6301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 6361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 6421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 6481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 6541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 6601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 6661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 6721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 6781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 6841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 6901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 6961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 7141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 7201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 7261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 7321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 7381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 7441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 7501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 7561 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat 7621 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 7681 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 7741 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 7801 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 7861 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 7921 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 7981 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat 8041 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 8101 cgccagtcga ccatagccaa ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg 8161 tggatctgga cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg 8221 tggatctgga ccccagccaa ttcaatatgg cggacttggc accatgccaa ttcaatatgg 8281 cggacttggc actgtgccaa ctggggaggg gtctacttgg cacggtgcca agtttgagga 8341 ggggtcttgg ccctgtgcca agtccgccat attgaattgg catggtgcca ataatggcgg 8401 ccatattggc tatatgccag gatcaatata taggcaatat ccaatatggc cctatgccaa 8461 tatggctatt ggccaggttc aatactatgt attggcccta tgccatatag tattccatat 8521 atgggttttc ctattgacgt agatagcccc tcccaatggg cggtcccata taccatatat 8581 ggggcttcct aataccgccc atagccactc ccccattgac gtcaatggtc tctatatatg 8641 gtctttccta ttgacgtcat atgggcggtc ctattgacgt atatggcgcc tcccccattg 8701 acgtcaatta cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa taggaccacc 8761 caccattgac gtcaatggga tggctcattg cccattcata tccgttctca cgccccctat 8821 tgacgtcaat gacggtaaat ggcccacttg gcagtacatc aatatctatt aatagtaact 8881 tggcaagtac attactattg gaaggacgcc agggtacatt ggcagtactc ccattgacgt 8941 caatggcggt aaatggcccg cgatggctgc caagtacatc cccattgacg tcaatgggga 9001 ggggcaatga cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg 9061 gaggtctata taagcaatgc tcgtttaggg aac //