LOCUS 40924_36923 3469 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector PRFP, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3469) AUTHORS Zhang J. TITLE Direct Submission JOURNAL Submitted (17-AUG-2016) Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China REFERENCE 2 (bases 1 to 3469) TITLE Direct Submission REFERENCE 3 (bases 1 to 3469) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (17-AUG-2016) Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China" COMMENT SGRef: number: 2; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3469 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 415..433 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 491..1177 /codon_start=1 /label=TagRFP /note="monomeric derivative of red fluorescent protein from Entacmaea quadricolor (Merzlyak et al., 2007)" /translation="ELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVVE GGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ DTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALKLV GGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYCDL PSKLGHK" primer_bind complement(1248..1264) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1272..1288) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1296..1326) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1341..1362) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1650..2238) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2412..3269) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQAQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFLADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAAIGAS LIKHW" promoter complement(3270..3374) /label=AmpR promoter ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgtaatac 421 gactcactat agggcgacat atgatcgatg atatcccatg ggcggccgcc tgcagaccag 481 gtctatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt 541 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca 601 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc 661 taccagcttc atgtacggca gcagaacctt catcaaccac acccagggca tccccgactt 721 ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg 781 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt 841 caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg 901 ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaagcga 961 catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttcaaga ccacatacag 1021 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg accacagact 1081 ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc 1141 cagatactgc gacctcccta gcaaactggg gcacaagtga agactggaga tctggatccc 1201 tcgagtctag agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt 1261 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 1321 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 1381 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 1441 gggagaggcg gtttgcgtat tgggcgctct tccgcttccg cgctcactga ctcgctgcgc 1501 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 1561 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 1621 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 1681 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 1741 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 1801 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg 1861 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 1921 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 1981 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2041 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 2101 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2161 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2221 agaaaaaaag gatctcaaga agatcctttg atcttttcga cggggtctga cgctcagtgg 2281 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 2341 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 2401 tctgacagtt accaatgctt aatcagtgag gcacctatcg cagcgatctg tctatttcgt 2461 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 2521 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 2581 gcaagaaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 2641 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 2701 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 2761 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 2821 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 2881 ttatcactca tggtgatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 2941 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 3001 ccgagttgcg cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 3061 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3121 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 3181 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 3241 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 3301 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 3361 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 3421 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc //