LOCUS       .                       5664 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1610..2326
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     polyA_signal    2452..2573
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2580..3035)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3062..3166
                     /label="AmpR promoter"
     promoter        3168..3525
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3560..4351
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    4586..4633
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4962..5550
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa taatgcctgc aggtcaattc tgagggcaga gccgggccac tttctcaggc
       61 ctctgatttc atactgtggt gttagttact tctgagagga cagcttgctg ccagagctct
      121 attttttatg ttagaggctc cttctgcctg cagactctgc tgtctgggaa gggcacagcg
      181 ttaggaggga gagggaggtg tgagtccctc cgtggacccg ctgctttgta cttctctatc
      241 tcatttcctt ttcagcacca ctctgggaaa tcagtattcc agccccattt tatcctcaga
      301 aaattgaggc tctgagatgt tatctctgtg acctgggtcc tattacgtgc caaaggcatc
      361 atttaagcct aagatgtcct ggctccaagg tgtcagcatc tggaagacag gcgccctcat
      421 cctgccatcc ctgctgcggc ttcactgtgg gcccagggga catctcagcc ccgagaaggg
      481 tcagcggccc ctcctggacc accgactccc cgcagaactc ctctgtgccc tctcctcacc
      541 agaccttgtt cctcccagtt gctcccacag ccagggggca gtgagggctg ctcttccccc
      601 agccccactg aggaacccag gaaggtgaac gagagaatca gtcctggtgg gggctgggga
      661 gggccccaga catgagacca gctcctcccc caggggatgt tatcagtggg tccagagggc
      721 aaaataggga gcctggtgga gggaggggcc tgaaattcca gtgtgaaagg ctgagatggg
      781 cccgaggccc ctggcctatg tccaagccat ttcccctctc accagcctct ccctggggag
      841 ccagtcagct aggaaggaat gagggctccc caggcccacc ccagttcctg agctcatctg
      901 ggctgcaggg ctggcgggac agcagcgtgg actcagtctc ctagggattt cccaactctc
      961 ccgcccgctt gctgcatctg gacaccctgc ctcaggccct catctccact ggtcagcagg
     1021 tgacctttgc ccagcgccct gggtcctcag tgcctgctgc cctggagatg atataaaaca
     1081 ggtcagaacc ctcctgcctg tctgctcagt tcatccctag aggcaggact ctagaggatc
     1141 tgcgatcgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
     1201 cggtaccgcc accatgccag agccagcgaa gtctgctccc gccccgaaaa agggctccaa
     1261 gaaggcggtg actaaggcgc agaagaaagg cggcaagaag cgcaagcgca gccgcaagga
     1321 gagctattcc atctatgtgt acaaggttct gaagcaggtc caccctgaca ccggcatttc
     1381 gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg
     1441 tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca
     1501 gacggccgtg cgcctgctgc tgcctgggga gttggccaag cacgccgtgt ccgagggtac
     1561 taaggccatc accaagtaca ccagcgctaa ggatccaccg gtcgccacca tggtgagcaa
     1621 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     1681 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     1741 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     1801 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     1861 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     1921 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     1981 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     2041 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     2101 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     2161 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     2221 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     2281 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga
     2341 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     2401 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     2461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta
     2581 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2641 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2701 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     2761 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2821 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     2881 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     2941 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     3001 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     3061 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3121 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     3181 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3241 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3481 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     3541 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     3601 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     3661 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     3721 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     3781 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     3841 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     3901 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     3961 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4021 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4081 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4141 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4201 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4261 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4321 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     4381 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     4441 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     4501 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     4561 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     4621 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     4681 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     4741 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     4801 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4861 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5101 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5641 gataaccgta ttaccgccat gcat
//
