LOCUS V034712 11538 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V034712 VERSION V034712 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" promoter 2221..2432 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" intron 2433..3371 /note="intron upstream of the start codon of human EF-1α" /label="EF-1α intron A" CDS 3393..5456 /label="Siglecg(NM_172900)" /note="Siglecg(NM_172900)" /gene="Siglecg" CDS 5466..6215 /codon_start=1 /note="more active than the original APEX due to the A134P mutation" /product="soybean ascorbate peroxidase, engineered to serve as a monomeric tag for electron microscopy and live-cell proteomics (Lam et al., 2015)" /transl_table=1 /translation="MGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAG TFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVA VEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGH TIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVD KYAADEDAFFADYAEAHQKLSELGFADA" /label="APEX2" misc_feature 6332..6881 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES" CDS 6884..7483 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7507..8095 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8302..8935 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(9063..9079) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9087..9103 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9111..9141) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9156..9177 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9465..10053) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10224..11084) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11085..11189) /gene="bla" /label="AmpR promoter" polyA_signal 11237..11371 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt 2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc 2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt 2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg 2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 3361 ttccatttca ggtgtcgtga ggatccgcca ccatgtcact gctgctgttc ctgctgtcct 3421 tcctgttgga tgggcctcaa ggtcagatgg agagctactt cctacaggtg cagagaattg 3481 tgaaggcaca ggagggtctg tgcatcttcg tgccctgctc cttctcctcc cctgagggaa 3541 aatggcttaa ccgttcccca ctttatggct actggttcaa aggcatcaga aaaccatcac 3601 tttcatttcc agtggccaca aataacaaag ataaagtgtt agaatgggaa gcccgagggc 3661 gcttccagct cttgggggat atctctaaaa agaactgttc cttgctaatc aaagatgttc 3721 agtggggaga ctcaacaaac tatttcttcc ggatggagag aggatttgag agattcagtt 3781 tcaaggagga gttcaggcta caagtggaag ccctgactca gaagccagat atcttcattc 3841 ctgaggtcct ggagcctggg gagccagtga ccgttgtctg cttgttttcc tggaccttca 3901 accaatgccc agctccttct ttctcctgga tgggggatgc tgtctccttc caagaaagca 3961 gaccgcacac atccaattac tcagttctga gctttatccc aggacttcaa caccatgata 4021 ctgagctcac atgtcagctg gacttctcta gaatgagcac acaaaggact gtccgactaa 4081 gagtggccta tgcccccaga tctcttgcta tcagcatttt ccatgacaat gtatcagtcc 4141 cagacttgca tgagaatcct tcacatctgg aagttcagca aggccagtct cttcgcctcc 4201 tctgtactgc tgacagccag ccccctgcta cactgagctg ggtcctagag gaccaagtcc 4261 tttcttggtc tagccctgtg gggtcgagaa ccctggcact agagctgccc tgggtgaaag 4321 ctggggactc tggacactac acttgccaag cagagaatag gctgggctcc cagcaacata 4381 ccctggacct ctctgtgctg taccccccac aggacctgag agtgactgtt tcccaagcaa 4441 acaggacagt gttggaaatc ctcaggaatg ccatctccct cccagtcctg gagggccaaa 4501 gcctgtgcct agtctgtgtc acctatagca atcccccagc caatgtgagt tgggcttggg 4561 tgacacagac cctgatccca atccagtctt cagagcctgg ggtcctggag ctgcctctgg 4621 tccagagaga acatgaagga gaattcacct gtgctgcaca gaacccactg ggtgcccagc 4681 gcatctctct gagcctctct gtgcactacc cgccccagat gtccagcccc tcctgctcct 4741 gggaggccaa gggtctgcac tgcaactgct cctccagagc ctggcctgcc ccctccctgc 4801 gctggcggct gggggagggg ctgctggagg ggaacagcag caatgcctcc ttcacagtca 4861 ctttcagctc acttggaccc tgggtcaaca gctccctgag cctccttcag gagctggggc 4921 ccagcctctg gctcagctgt gagtcctgga acacccatgg agcccagacc acctctgtcc 4981 tgctgctacc tgataaagac agtgccacag cattctccaa gggagcagtt ttgggctttg 5041 gcatcacagc cctccttgcc ctgtgcctca tcgtggtcat cgtgaagacc ctccagaaga 5101 aaggaaccca ggaggaaccc tccaggccca agctctcccg gggcagcacg atcctggact 5161 acatcaatgt ggtccccaag accaggtccc tggctcggaa ttggaaagct gaaccagatg 5221 ccccttctag gagctcaccc ctggacactc acttccctaa accaaagaag aagcaaaagg 5281 acccacattt cacgtaccct ggttgcccag atcccacatc atcctctcag gtcccagtct 5341 ctgagaataa cccggaagaa ctccactatg ctgctctcaa cttctcccgc ctgagactgc 5401 aggagaccca ggatccccaa gacacctatt ctgattacac agaggtcaga gtccacggga 5461 gatctatggg aaagtcttac ccaactgtga gtgctgatta ccaggacgcc gttgagaagg 5521 cgaagaagaa gctcagaggc ttcatcgctg agaagagatg cgctcctcta atgctccgtt 5581 tggcattcca ctctgctgga acctttgaca agggcacgaa gaccggtgga cccttcggaa 5641 ccatcaagca ccctgccgaa ctggctcaca gcgctaacaa cggtcttgac atcgctgtta 5701 ggcttttgga gccactcaag gcggagttcc ctattttgag ctacgccgat ttctaccagt 5761 tggctggcgt tgttgccgtt gaggtcacgg gtggacctaa ggttccattc caccctggaa 5821 gagaggacaa gcctgagcca ccaccagagg gtcgcttgcc cgatcccact aagggttctg 5881 accatttgag agatgtgttt ggcaaagcta tggggcttac tgaccaagat atcgttgctc 5941 tatctggggg tcacactatt ggagctgcac acaaggagcg ttctggattt gagggtccct 6001 ggacctctaa tcctcttatt ttcgacaact catacttcac ggagttgttg agtggtgaga 6061 aggaaggtct ccttcagcta ccttctgaca aggctctttt gtctgaccct gtattccgcc 6121 ctctcgttga caaatatgca gcggacgaag atgccttctt tgctgattac gctgaggctc 6181 accaaaagct ttccgagctt gggtttgctg atgcctagga attcctcgag ggcggccgct 6241 ctagagtcga cgggccgcgg taacaattgt taactaactt aagctagcaa cggtttccct 6301 ctagcgggat caattccgcc ccccccccct aacgttactg gccgaagccg cttggaataa 6361 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg 6421 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc 6481 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct 6541 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 6601 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc 6661 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta 6721 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg 6781 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg 6841 aaccacgggg acgtggtttt cctttgaaaa acacgataat accatgaccg agtacaagcc 6901 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 6961 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 7021 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 7081 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 7141 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 7201 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 7261 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 7321 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 7381 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 7441 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacccgggc ggggcgcgtc 7501 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 7561 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 7621 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 7681 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 7741 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 7801 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 7861 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 7921 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 7981 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 8041 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga 8101 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag 8161 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag 8221 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 8281 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc 8341 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag 8401 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc 8461 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc 8521 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag 8581 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag 8641 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg 8701 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct 8761 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 8821 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 8881 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 8941 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 9001 gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca 9061 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 9121 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 9181 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 9241 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 9301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 9361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 9421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 9481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 9541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 9601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 9661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 9721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 9781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 9841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 9901 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 9961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 10021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 10081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 10141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 10201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 10261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 10321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 10381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 10441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 10501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 10561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 10621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 10681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 10741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 10801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 10861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 10921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 10981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 11041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 11101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 11161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 11221 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag 11281 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 11341 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca 11401 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact 11461 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg 11521 tactacaaac ttagtagt //