LOCUS       V038722                11772 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038722
VERSION     V038722
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2221..2432
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     intron          2433..3371
                     /note="intron upstream of the start codon of human EF-1α"
                     /label="EF-1α intron A"
     regulatory      3387..3396
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3396..3425
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3426..6470
                     /label="Opa1(NM_001403170)"
                     /note="Opa1(NM_001403170)"
                     /gene="Opa1"
     misc_feature    6566..7115
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             7118..7717
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7741..8329
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8536..9169
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9297..9313)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9321..9337
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9345..9375)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9390..9411
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9699..10287)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10458..11318)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11319..11423)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11471..11605
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga acgcgtgcca ccatggaaca aaaactcatc tcagaagagg
     3421 atctgatgtg gcgagcaggt cgggcggccg tggcctgtga agtctgccaa tccttagtga
     3481 aacacagttc tggaatacaa agaaacgtac cgctccaaaa actccatctg gtttcacgaa
     3541 gtatttatcg ttcacatcat cctgccctca agcttcaaag accccaacta aggacaccat
     3601 ttcagcagtt ctcttctcta actcaccttt cattacataa attgaaactt tctccaatta
     3661 aatatggcta ccagccccgc aggaactttt ggccagcaag gttagctgca agactcttaa
     3721 aacttcgata tatcatactg ggatctgctg ttggaggtgg ctatacagcc aaaaaaacct
     3781 tcgatgaatg gaaagatatg ataccagacc ttagtgacta taagtggatt gtgcctgact
     3841 ttatatggga aattgatgag tatattgatt tggagaaaat tagaaaagcc ctgcccagct
     3901 cagaagacct tgccagttta gctcccgacc tggacaagat tactgagagc ctcagcttgt
     3961 tgaaggactt cttcactgca ggtcccaaat tggttagtga agtcctagaa gtttctgagg
     4021 cccttctctt gttaggttca cctggagaaa cagcatttcg agcaacagat catggatctg
     4081 aaagtgacaa gcattacagg aagggtctgc ttggtgagct cattctctta caacagcaaa
     4141 ttcaagagca cgaagaggaa gcacgcagag ccgctggcca gtatagcacg agctatgccc
     4201 aacagaagcg caaggtgtca gacaaagaaa agattgacca acttcaagaa gaacttctgc
     4261 atactcagtt aaagtatcag aggatcttgg agcgcctgga aaaggagaac aaagagctgc
     4321 ggaagctggt gctgcagaag gacgacaaag gcatccacca caggaagctc aagaaatctt
     4381 tgattgatat gtattctgaa gttcttgatg ttctttctga ttatgatgcc agttacaata
     4441 cacaagatca cctaccacgg gttgttgtgg ttggagatca gagtgctggg aaaaccagtg
     4501 tgctggaaat gattgctcag gcccggatct tcccgagagg gtccggcgag atgatgacac
     4561 gctctccagt gaaggtgact ctcagtgaag gccctcacca tgtggccttg tttaaagata
     4621 gctctcggga atttgatctc accaaggagg aagatcttgc agcattaaga catgaaatcg
     4681 aactccgaat gaggaaaaat gtgaaagaag gttgtactgt tagccccgag accatatctc
     4741 taaatgtcaa aggccctggg ctgcagagga tggtgctcgt ggacttgcct ggtgtcatca
     4801 acaccgtgac atcaggcatg gctcccgaca caaaggaaac tattttcagt atcagcaaag
     4861 cttacatgca gaatcctaac gccatcatcc tgtgcatcca agacggatcc gtagatgctg
     4921 agcgcagtat tgttacagac ttggtcagtc aaatggatcc tcatggaaga agaaccatat
     4981 ttgttttgac caaagtagac ctggcagaaa aaaatgtagc cagtccaagc aggatacaac
     5041 agataattga aggcaagctc ttcccaatga aagctctggg ttattttgct gtcgtaacag
     5101 gaaaaggaaa cagctctgaa agtattgaag ctataagaga gtatgaagag gaattttttc
     5161 agaattcaaa actgctaaag acaagcatgc taaaggcaca ccaggtcacc acgagaaatc
     5221 tcagccttgc tgtgtcagac tgcttttgga aaatggttcg agagtcagtt gaacaacagg
     5281 ctgatagttt taaagccacg cgctttaacc tagagacgga atggaagaat aactacccgc
     5341 gcctgcgaga gctcgacagg aatgaactct ttgaaaaagc taaaaatgag atcctcgatg
     5401 aggtcatcag tctgagccag gtcacgccaa agcactggga ggaaatcctg cagcaatccc
     5461 tgtgggaacg agtgtcaaca catgtgattg agaacatcta ccttccagct gcccagacca
     5521 tgaattcggg aacatttaac accacagtag acatcaagct taaacagtgg actgacaagc
     5581 agcttcctaa taaagcagtc gaggttgcct gggagactct acaagaggaa ttttcccgct
     5641 tcatgacaga acccaaagga aaggaacacg acgacatatt tgacaaactt aaggaggctg
     5701 tgaaggagga gagtatcaag cggcacaagt ggaacgactt tgccgaggat agcttgaggg
     5761 ttattcagca caatgctttg gaagaccggt ccatatcaga taagcaacag tgggatgcag
     5821 ccatttactt catggaagag gcgcttcaag gtcgtctcaa ggatactgaa aatgctattg
     5881 aaaacatgat tgggccagac tggaaaaaga ggtggatgta ctggaagaat cggacccaag
     5941 agcagtgtgt tcacaacgaa accaagaacg agttggagaa gatgctgaag gttaatgatg
     6001 agcacccagc ttacctggca agtgatgaga ttaccacagt ccggaagaac ctggagtctc
     6061 gaggagtgga agtcgatcca agcttgatta aggatacttg gcatcaagtt tatagaagac
     6121 atttcttaaa aacagctcta aatcattgta acctttgtcg cagaggtttt tattactacc
     6181 agaggcattt tatagattct gagctggaat gcaatgacgt ggtcctgttt tggcgaatac
     6241 agcgcatgct cgctatcact gccaatacat taaggcagca gcttacaaac actgaagtta
     6301 ggcgactaga gaaaaacgtt aaagaggtat tagaagattt tgcagaagac ggtgagaaga
     6361 aggttaaatt gctcactggc aaacgagttc agctggcaga agatctcaag aaagttagag
     6421 aaattcaaga aaagcttgat gctttcattg aagctcttca ccaggagaag tagtctagag
     6481 tcgacgggcc gcggtaacaa ttgttaacta acttaagcta gcaacggttt ccctctagcg
     6541 ggatcaattc cgcccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6601 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     6661 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     6721 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     6781 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     6841 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     6901 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     6961 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     7021 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     7081 ggggacgtgg ttttcctttg aaaaacacga taataccatg accgagtaca agcccacggt
     7141 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     7201 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     7261 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     7321 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     7381 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     7441 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     7501 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     7561 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7621 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7681 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccc gggcggggcg cgtctggaac
     7741 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7801 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7861 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7921 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7981 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8041 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8101 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     8161 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8221 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8281 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
     8341 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     8401 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     8461 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8521 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     8581 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     8641 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     8701 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     8761 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     8821 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     8881 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     8941 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     9001 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     9061 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9121 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     9181 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     9241 ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca
     9301 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9361 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9421 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9481 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9541 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9601 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9661 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9721 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9781 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9841 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    11461 tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    11521 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    11581 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    11641 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
    11701 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
    11761 aaacttagta gt
//
