LOCUS       V038849                12924 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038849
VERSION     V038849
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2221..2432
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     intron          2433..3371
                     /note="intron upstream of the start codon of human EF-1α"
                     /label="EF-1α intron A"
     regulatory      3387..3396
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3396..3425
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3426..7601
                     /label="Lrpprc(NM_028233)"
                     /note="Lrpprc(NM_028233)"
                     /gene="Lrpprc"
     misc_feature    7718..8267
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             8270..8869
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8893..9481
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9688..10321
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(10449..10465)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10473..10489
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10497..10527)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10542..10563
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10851..11439)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11610..12470)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12471..12575)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    12623..12757
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga acgcgtgcca ccatggaaca aaaactcatc tcagaagagg
     3421 atctgatggc agccctgctg agacccgcgc gttggctgct cggggccgcg gcggccccgc
     3481 gcctcccgct gtccctgcgc ctccctgcgg gcgtcccggg ccggctgtcc tccgtcgtcc
     3541 gggtcgcggc tgttggtagc cggccggctg caggagagcg tctgagccaa gccagattgt
     3601 atgccatcgt tgctgagaaa agggatcttc aagaggagcc tgctcctgtg agaaagaaca
     3661 gcagtcaatt tgactgggct ctgatgagac tggataattc tgtccggaga acaggccgca
     3721 tcacaaaggg gcttctgcag agagtctttg agagcacgtg tagctcaggt agcccaggga
     3781 gcaatcaagc tctgcttctg ctgcgcagct gtggctcgct cctgcccgaa ctgagtctcg
     3841 ccgagaggac agagtttgct cacaagatct gggacaaact tcagcagtta ggtgtcgtat
     3901 atgatgtcag tcattacaat gctttactta aagtatatct tcaaaatgaa tacaaatttt
     3961 cacctactga cttcctggca aagatggagg gagcaaacat ccaaccaaat cgagtaacat
     4021 accagaggct gatagctgcc tactgtaatg ttggggacat tgaaggtgcc agcaagatcc
     4081 ttggatttat gaaaacgaaa gaccttccga tcacagaggc cgtgttcagt gctctcgtca
     4141 cagggcatgc gagagctggg gatatggaaa atgcagaaaa tattctcaca gtgatgaaac
     4201 aggccggcat tgagcctggc ccagacacgt atctggcctt gttgaatgca catgctgaga
     4261 ggggtgacat tggccaggtt aggcagattc tggagaaagt ggagaagtca gaccattact
     4321 tcatggaccg cgacttcttg caggttattt ttagcttcag taaggctggc taccctcagt
     4381 atgtctcaga aatactggag aagattacct atgagagacg gtctattcca gatgcaatga
     4441 acctcatttt gtttttagcc actgagaagt tagaagacac tgcgttccag gttttattgg
     4501 cattacccct gtccaaggac gagagctccg ataactttgg cagtttcttt ttgcggcact
     4561 gtgtgactct ggatttgccc cctgagaagc tgatagacta ctgtcggagg ctgagggacg
     4621 ccaagctgca cagctcctca ctgcagttca cgctgcactg tgctcttcaa gccaatagga
     4681 cagctttggc aaaagcagtg atggaggctt tgagggaaga agggtttcct atccgaccgc
     4741 actatttctg gccgttgctt gctgggcatc agaaaacaaa aaatgttcaa ggaataatag
     4801 atatcctcaa aataatgaac aaagtgggag tggatcctga tcaggaaaca tatataaact
     4861 atgtgtttcc gtgctttgat agtgcacagt cagttcgagc tgctttgcag gaaaatgaat
     4921 gtctcctcgc aagtagtacc tttgctcaag ctgaagtgaa gaatgaagca ataaatggga
     4981 acttacagaa cattttgtca tttttggaat cgaatacatt gcctttctcg tttagttctt
     5041 tgagaaacag cctaatccta ggcttcagga ggtcgatgaa catagatctt tggagcaaga
     5101 taacagaatt gttgtacaag gatgaacgct attgctcaaa gcctccggga ccagcggaag
     5161 ctgttggcta ttttctttat aacttgattg acagcatgag tgactcagag gtacaggcca
     5221 aggaggagcg tttgagacaa tacttccatc agctgcagga gatgaatgtt aaagttcctg
     5281 aaaacatcta caaaggcatt tgtaatttgc tgaataccta ccatgttcct gaattgatta
     5341 aggatattaa ggttctggtt gacagagaga aggtagattc tcaaaaaact tctcaagtta
     5401 cctcatctga tttggaatca acacttgaga aactcaaagc tgaaggccaa cctgtaggat
     5461 ctgccctgaa gcagctcctg ctgctgctct gctcagagga gaatatgcaa aaggcccttg
     5521 aggtgaaagc aaaatatgag tcagacatgg ttattggtgg ctatgcagca ttaataaatt
     5581 tgtgctgtcg acatgataat gcagaagatg cgtggaactt gaaacaagaa gttgaccgct
     5641 tagatgcttc ggctattctt gacactgcca agtacgtagc ccttgtaaaa gtactgggaa
     5701 agcacagcag actccaagat gctattaaca ttctaaagga gatgaaagag aaggatgttg
     5761 ttatcaaaga tgcaacagtc ttgtcctttt tccacatcct caatggtgca gctttaagag
     5821 gtgaaattga aacagtaaaa cagctgcatg aagccatcgt gactcttggg ttggcaaagc
     5881 cgtccagcaa cataagcttc ccgttggtca ctgtgcacct ggaaaagggt gacttacctg
     5941 ctgctcttga agccagcatt gcctgccata aaaaatataa agtgttaccc aggattcatg
     6001 atgtcttatg taagctagta gagaaaggcg agactgattt gatccagaaa gcaatggact
     6061 ttgtgagcca agaacaaggg gagatgacga tgctctacga cctcttcttt gctttcctgc
     6121 agacggggaa ttacaaagaa gctaagaaga tcattgagac tccaggcatt agagctcggc
     6181 ctacaagact ccagtggttt tgtgatcgat gcattgccag taatcaggtt gaagctcttg
     6241 agaagttggt agagctgact gagaagctgt ttgagtgtga cagagaccag atgtactaca
     6301 acttactgaa gctatacaaa ataagcagtg actggcaaag agcggatgct gcgtggacca
     6361 aaatgcaaga agagaacatt atccctcgag agcggacact gcggctctta gccgagatct
     6421 tgaaaaccag caaccaggaa gttcctttcg acgttccgga gttgtggttt ggagatgaca
     6481 gaccttccct gagtccatcc tcacgctcag caggagagga cgttactgag aagacgttgt
     6541 tgtctaactg caaactaaag aagagtaaag atgcatataa tatcttcctt aaagccgaaa
     6601 agcaaaacgt tgtatttagc agtgaaactt atagcacctt gataggcttg ctgctgagta
     6661 aggacgactt cacccaagca atgcacgtga aggatttcgc tgagacccac atcaagggct
     6721 tcacactgaa cgatgctgcc aacagcctcc tcatcataag gcaagttagg cgggattatt
     6781 tgaaaggggc tctggcaact ctgagagcag ccttggattt gaagcaggtt ccgtcccaga
     6841 tcgccgtgac ccgcctcatc caggcgttgg ccttgaaggg tgatgtggaa agcatagagg
     6901 ccattcagag aatggtggct ggacttgaca cgattggact ctcaaaaatg gtttttatca
     6961 ataacatcgc tttggcccag atgaagaata ataaacttga tgctgccata gaaaacattg
     7021 agcacctgct tgcttccgag aaccaagcca tagaacctca gtactttggc ttgtcgtatc
     7081 tattcagaaa agtgatcgaa gagcagatgg aaccagcgct agagaagtta agcatcatgt
     7141 ctgagagaat ggcgaatcag tttgcacttt acaagcccgt cactgatcta ttcctgcagc
     7201 ttgtggattc aggcaaggtg gatgaggcca gagctctctt agagagatgc ggtgccattg
     7261 ccgagcagag ctcgcttctg tcggtgttct gtctgaggac ttctcagaaa ccgaaaaagg
     7321 caccagttct gaagactttg ttagaactga ttcctgagtt acgtgataac gataaagtat
     7381 attcttgcag catgaaaagc tatgccttag acaaagatgt ggcctcggct aaagcactgt
     7441 atgagtattt gacagccaag aacttgaagc tagatgacct gtttctcaag cgctatgcag
     7501 ctttgctcaa ggatgtcggc gaaccagtcc ccttccccga gccccctgaa agctttgcat
     7561 tttatataaa gcaactaaag gaagcaaggg aaagcccttc ataggaattc ctcgagggcg
     7621 gccgctctag agtcgacggg ccgcggtaac aattgttaac taacttaagc tagcaacggt
     7681 ttccctctag cgggatcaat tccgcccccc ccccctaacg ttactggccg aagccgcttg
     7741 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     7801 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     7861 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     7921 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     7981 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     8041 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     8101 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     8161 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     8221 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tgaccgagta
     8281 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     8341 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     8401 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8461 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     8521 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8581 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8641 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8701 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8761 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     8821 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac ccgggcgggg
     8881 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8941 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     9001 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     9061 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     9121 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     9181 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     9241 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     9301 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9361 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9421 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9481 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     9541 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     9601 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     9661 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     9721 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     9781 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     9841 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     9901 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     9961 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
    10021 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
    10081 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
    10141 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
    10201 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
    10261 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
    10321 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
    10381 gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg
    10441 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    10501 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    10561 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    10621 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    10681 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    10741 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    10801 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10861 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10921 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10981 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    11041 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    11101 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    11161 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    11221 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    11281 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    11341 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    11401 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    11461 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    11521 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    11581 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    11641 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    11701 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    11761 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    11821 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    11881 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11941 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    12001 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    12061 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    12121 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    12181 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    12241 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    12301 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    12361 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    12421 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    12481 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    12541 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    12601 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
    12661 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12721 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    12781 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    12841 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    12901 aatatgtact acaaacttag tagt
//
