LOCUS       V040939                11685 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040939
VERSION     V040939
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2221..2432
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     intron          2433..3371
                     /note="intron upstream of the start codon of human EF-1α"
                     /label="EF-1α intron A"
     regulatory      3387..3396
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3396..3425
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3432..6362
                     /label="Ap2a1(NM_007458)"
                     /note="Ap2a1(NM_007458)"
                     /gene="Ap2a1"
     misc_feature    6479..7028
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             7031..7630
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7654..8242
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8449..9082
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9210..9226)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9234..9250
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9258..9288)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9303..9324
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9612..10200)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10371..11231)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11232..11336)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11384..11518
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca ccatggaaca aaaactcatc tcagaagagg
     3421 atctgacgcg tatgccggct gtatccaaag gcgatggcat gcgtgggctc gccgtgttca
     3481 tctccgacat ccggaactgc aagagcaaag aggctgagat caagaggatc aacaaggaac
     3541 tggccaacat ccgttccaag ttcaaagggg acaaggcctt ggatggctac agtaaaaaga
     3601 agtatgtgtg taagctgctc ttcatattcc tgcttggcca tgacattgac tttggacata
     3661 tggaggccgt gaacctgcta agctctaaca agtacacgga gaagcagata gggtacctgt
     3721 tcatctcagt cctggtgaac tcgaactcag aactgatccg gctcatcaac aacgctatca
     3781 agaatgacct ggccagtcgc aaccccacct tcatgtgcct ggccttgcac tgtatcgcta
     3841 acgtgggcag ccgtgagatg ggcgaggctt ttgctgcaga cattccccga atcctggtgg
     3901 ctggggacag catggacagt gtgaagcaga gtgccgccct atgcctactg cgactctaca
     3961 aggcctcgcc cgacttggtg cccatgggcg agtggacggc acgtgtagtg cacttgctca
     4021 atgatcagca catgggagtg gtcacagctg ctgtcagcct catcacctgt ctctgcaaga
     4081 agaatccgga tgacttcaag acctgtatct ccctggctgt gtctcgctta agccggatcg
     4141 tctcctcagc ctccactgac ctccaggact acacttacta cttcgttcct gcaccctggc
     4201 tctctgtgaa gctactgcgg ctgctccagt gttacccacc accagaggat gcagccgtga
     4261 aagggcggtt agtggagtgt ctggagactg tgctcaacaa ggcccaggag cctcccaagt
     4321 ccaagaaggt gcagcactcc aacgccaaga acgctatcct ctttgagacc attagcctca
     4381 tcatccacta tgacagtgag cccaacctcc tggtccgcgc ctgcaaccag ctgggccagt
     4441 tcctgcagca ccgggagact aacctgcgct acctggccct ggagagcatg tgcacgctgg
     4501 ccagctccga gttctcccac gaggccgtca agacccacat tgatacagtt attaatgccc
     4561 tcaagacgga gcgggacgtc agtgtgaggc agcgggcggc tgatctcctg tatgccatgt
     4621 gtgaccggag caatgccaag cagattgtgt cagagatgct gcggtacctg gagactgctg
     4681 actatgccat ccgagaggag atcgtgctga aggtggccat cctggctgag aagtatgcag
     4741 tggactacag ctggtacgtg gacaccatcc tcaacctcat ccgcatcgcg ggcgactatg
     4801 tgagcgagga ggtgtggtac cgcgtgctgc agatcgtcac caaccgtgat gacgtccagg
     4861 gttatgctgc caagacagtg tttgaggccc tccaggcccc agcctgtcat gagaacatgg
     4921 tgaaggtcgg tggctacatc cttggggagt ttgggaactt gattgctggg gacccacgct
     4981 ccagcccacc agtgcagttc tcgctgctgc actccaagtt ccacctgtgc agcgtggcca
     5041 cccgcgctct gttgctgtcc acctacatca agttcatcaa cctcttccct gagaccaagg
     5101 ccaccatcca aggggttctg cgtgccggct cccagctgcg aaatgccgac gtggagctac
     5161 agcagcgggc cgtggagtac ctcaccctca gctccgtagc cagcaccgat gttctggcta
     5221 cggtgctaga agaaatgccc ccatttcccg agcgggagtc gtccatcttg gccaagctga
     5281 agcgcaagaa gggccctggg gcagccagtg ccctagatga cagccgcagg gacaccagca
     5341 gcaatgacat caatgggggt gtggagccca cccccagcac tgtgtcgacc ccctcaccct
     5401 ccgcggacct cttagggctg cgggcagccc ctccccctgc tgcacccccg gctcccgtag
     5461 gcgggaacct cctggtggat gtcttctctg acggccccac tgcacagccc agcctggggc
     5521 ccactcctga ggaggccttc ctcagcgagc tggagccccc tgcccctgag agccccatgg
     5581 ctttgttggc tgacccagct ccagctgctg acccaggtcc tgaggacata ggccctccca
     5641 ttccagaagc agatgaactg ttgaataagt tcgtgtgtaa gaatagtggg gtcttgtttg
     5701 agaaccagct gctgcagatt ggagtcaagt ctgagttccg gcagaacctg ggccgcatgt
     5761 atctcttcta tggcaacaag acttctgtgc agttccagaa cttcttgccc accgtggtcc
     5821 atcctgggga cctccagact cagctggcgg tgcagaccaa gcgtgtggcg gcacaagtgg
     5881 acggtggcgc acaggtgcag caagtactca acattgagtg tctgcgagac ttcctgacgc
     5941 cgccactgtt gtcggtgcgc ttccggtacg gtggcaccgc ccagtccctc actctgaagc
     6001 tcccagtgac catcaacaaa ttcttccagc ccacagagat ggcggcccaa gactttttcc
     6061 agcgctggaa gcagctgagc ctccccctgc aggaggcaca gaaaatcttc aaagccaacc
     6121 acccgatgga tgctgaagtt actaaggcca agcttctggg gtttggctct gctcttctgg
     6181 acaatgtgga tcccaaccct gagaactttg tgggtgctgg aatcatccag acgaaggccc
     6241 tgcaggtggg gtgtctgctt cggctggagc ccaatgccca ggcccaaatg taccgtctaa
     6301 ccctgcgcac cagcaaagag cctgtctccc gtcacttgtg tgagctgctg gcccagcagt
     6361 tctgagaatt cctcgagggc ggccgctcta gagtcgacgg gccgcggtaa caattgttaa
     6421 ctaacttaag ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac
     6481 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     6541 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     6601 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     6661 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     6721 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     6781 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     6841 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     6901 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     6961 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     7021 cgataatacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     7081 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     7141 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     7201 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7261 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7321 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7381 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7441 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7501 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7561 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     7621 cggtgcctga cccgggcggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt
     7681 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7741 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7801 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7861 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7921 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7981 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8041 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     8101 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8161 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8221 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     8281 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     8341 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     8401 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     8461 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     8521 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     8581 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     8641 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     8701 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     8761 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     8821 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     8881 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8941 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9001 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9061 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     9121 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg
     9181 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9241 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9301 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9361 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9421 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9481 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9541 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9601 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9661 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9721 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9781 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9841 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9901 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9961 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10021 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    10081 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10141 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    10201 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10261 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10321 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10381 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10441 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10501 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10561 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10621 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10681 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10741 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10801 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10861 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10921 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10981 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    11041 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    11101 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    11161 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    11221 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    11281 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11341 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
    11401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    11521 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    11581 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    11641 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
