LOCUS       40924_25851        7724 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector PJAC98, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
  PUBMED    24032656
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7724)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    434..1934
                     /label=CG TRP1 w promoter and terminator
                     /note="CG TRP1 w promoter and terminator"
     misc_feature    1941..2162
                     /label=8x Ada operator
                     /note="8x Ada operator"
     regulatory      2163..2409
                     /label=PCyc1 promoter
                     /note="PCyc1 promoter"
                     /regulatory_class="promoter"
     CDS             2428..3135
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     regulatory      3180..3874
                     /label=C. albicans TadH1 terminator
                     /note="C. albicans TadH1 terminator"
                     /regulatory_class="terminator"
     promoter        complement(4272..4290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4297..4313)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4454..4909
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5547..5651
                     /label=AmpR promoter
     CDS             5652..6509
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6683..7271
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7559..7580
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7595..7625
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7633..7649
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7657..7673
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7694..7712
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccata tttctaccaa tctctcaaca
       61 ctgagtaatg gtagttataa gaaagagacc gagttaggga cagttagagg cggtggagat
      121 attccttatg gcatgtctgg cgatgataaa acttttcaaa cggcagcccc gatctaaaag
      181 agctgacagg gaaatggtca gaaaaagaaa cgtgcacccg cccgtctgga cgcgccgctc
      241 acccgcacgg cagagaccaa tcagtaaaaa tcaacggtta acgacattac tatatatata
      301 atataggaag catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc
      361 cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat
      421 cttgatccgg agcccgtcgc tttgagagaa atcagaagat tccaaaagtc tactgaactt
      481 ttgatcagaa agttgccttt ccaaagacta gtcagagaaa tcgcccaaga tttcaagacc
      541 gatctaagat tccaatcctc tgccatcggt gctctacagg aatccgttga agcttacttg
      601 gtctctttgt tcgaagacac caacttggcc gctatccacg ctaagcgtgt taccatccaa
      661 aagaaggata tcaagttggc tagaagattg agaggtgaaa gatcctaagc acacagggat
      721 tgttgtacaa ttacttttta tgattttaga tacacacaaa tcttttctac gattaccttt
      781 catttatttt cgtttcatgt tttattaatc attctttttg tatatttcac atcgggtggg
      841 gtttttatat taaattttaa taacataatc ttttaaagtt tctactttct atcctgtata
      901 ttatttccat atctatatct atattttcaa gcttgttcac atccttatcg gaattgttcc
      961 agcgtttttg tacagaaaat gatgttatgg taacgagcta tatcagacct actagtctta
     1021 tagagctgta cagagtcttg gaaacataac caacacacca acaagatgtc atttgattcg
     1081 ttactcgaca agaatgataa gctggtaaaa gtttgcggga ttcaaaccgt cgaggctgcc
     1141 gaaactgcgc ttcaagcagg cgctgatttg atagggatca tatgtgtccc caacaggaag
     1201 cggactatcg agagcgctgt ggctcgtgaa atatccaaat tgattcacaa atcaggtact
     1261 acaaagctgg tgggggtgtt caggaatcaa tctgttgagg acgtacatcg gctttctgag
     1321 gaatatgacc ttgacataat ccaattacat ggtgatgaat catggccaga gtactataac
     1381 gtcattaaga aaccaataat caaaagagtc atattcccta gagatgtcga tgttgtaaca
     1441 caagtgtgtc aaagaaaacc cttggtatgt ctaccattgt tcgactccga ggcaggcggt
     1501 acaggtgaaa agctggactg gtccagtatt tcctcctggg caagcgaaca gaataacgta
     1561 ttttatatac tagcgggtgg actaactgca gaaaacgtca tggaggcggt ggggttgcct
     1621 ggtgtaattg gtgttgacgt ctcaggtggt gtagagactg acggcgtcaa agacaatgac
     1681 aagataatta aatatgtaca aaatgcaaag aaacaatgaa ttacgaagag aagtctcaat
     1741 gaggttttac aaacaatcct ataatacata tatgccactt agatatactt tgaatactga
     1801 atatactgac tcattatatt ctttaattgt agtttctcaa aaatattcat acaggttagc
     1861 tatgaattca tgctataatt tccattttgt tcatgtaaga tacacatagg gataaaatgt
     1921 gataactaat cagcggtacc gggccgcatc ttgcgcttta atttttcggg ccgcatcttg
     1981 cgctttaatt tttcgggccg catcttgcgc tttaattttt cgggccgcat cttgcgcttt
     2041 aatttttcgg gccgcatctt gcgctttaat ttttcgggcc gcatcttgcg ctttaatttt
     2101 tcgggccgca tcttgcgctt taatttttcg ggccgcatct tgcgctttaa tttttcgggc
     2161 ccgagcagat ccgccaggcg tgtatatata gcgtggatgg ccaggcaact ttagtgctga
     2221 cacatacagg catatatata tgtgtgcgac gacacatgat catatggcat gcatgtgctc
     2281 tgtatgtata taaaactctt gttttcttct tttctctaaa tattctttcc ttatacatta
     2341 ggacctttgc agcataaatt actatacttc tatagacaca caaacacaaa tacacacact
     2401 aaattaataa tgactgaact cgagactagt aaaggagaag aacttttcac tggagttgtc
     2461 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     2521 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     2581 ctacctgttc catggcctac acttgtcact actttgtgtt atggtgttca atgcttttca
     2641 agatacccag atcacatgaa aaggcatgac tttttcaaga gtgccatgcc cgaaggttat
     2701 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
     2761 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     2821 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
     2881 atggcagaca aacaaaagaa tggaatcaaa gttaatttca aaactagaca caacattgaa
     2941 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     3001 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     3061 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     3121 atggatgaac tatacaatgg atcctgagta aataagcggc cgccaccgcg gtggagctct
     3181 aagcaaatag ctaaattata tacgaattaa tattatgatt aagtgtttac gtgagtgcga
     3241 tatttttatt actatcttat acagttgtat atactctata aaatgagttg tctattaatt
     3301 aacgcgatga atgctttctg ggtttacctc tccaacaact ctagtttact tctcaataca
     3361 ttcaattgta tttgatttgt caatacttca tcattaatca attctatagt tttgtttttc
     3421 tcgtttattt ccaaatttaa tgcatcaatt ttattattca atttgtcgtt gattttggtt
     3481 aatgatttta tggtttgatc tctggcattg attgtttgtg ttagtttttc attattgata
     3541 attaaattat ttaagttagt tatcaactcg gtgttttcaa gtttcaagtt ttcaatttct
     3601 ttagagttta ttagatttgt caaagtttct gaattgcttg attggtcctg tagaagagta
     3661 tttgttgttg tggataattg attcaatttt tgagacaatt gctggaaggc gttgaaatat
     3721 ctagcatcaa tctcatggtt tttttcccga gagtctcgta gattcaattg ttttaatata
     3781 tcttgggacc actcttgatt tgaactcatg gaaattaaac tgggtgttgt gttgtggtgt
     3841 aatgattgta ccccctttgc ttataattgt gtggttccga tgctgactta ctgggtatta
     3901 tatgtgtgcc caatagaaag agaacaattg acccggttat tgcaaggaaa atttcaagtc
     3961 ttgtaaaagc atataaaaat agttcaggca ctccgaaata cttggttggc gtgtttcgta
     4021 atcaacctaa ggaggatgtt ttggctctgg tcaatgatta cggcattgat atcgtccaac
     4081 tgcatggaga tgagtcgtgg cgagaatacc aagagttcct cggtttgcca gttattaaaa
     4141 gactcgtatt tccaaaagac tgcaacatac tactcagtgc agcttcacag aaacctcatt
     4201 cgtttattcc cttgtttgat tcagaaggcc ggccatttaa atgtttaaac ggccacgctg
     4261 gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
     4321 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     4381 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4441 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4501 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4561 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4621 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4681 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4741 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4801 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4861 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt
     4921 attttctcct tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat
     4981 taaattgaag ctctaatttg tgagtttagt attaggatct ctacccttgg cgaaaagtcc
     5041 tctgccaaca atgatgatat ctgatccacc acttacaact tcgtcgacgg ttctgtactg
     5101 ctgacccaat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt
     5161 ctgttcggag attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata
     5221 aaaaaaaaat gatgaattga aaaggtggta tggtgcactc tcagtacaat ctgctctgat
     5281 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     5341 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     5401 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     5461 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcggcgcg ccgaggacgt
     5521 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5581 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5641 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5701 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5761 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5821 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5881 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5941 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6001 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6061 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6121 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6181 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6241 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6301 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6361 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6421 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6481 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6541 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6601 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6661 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6721 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6781 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6841 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6901 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6961 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7021 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7081 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7141 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7201 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7261 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7321 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7381 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7441 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7501 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7561 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta
     7621 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7681 acgccaagct cggaattaac cctcactaaa gggaacaaaa gctg
//
