LOCUS       V014967                 4702 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014967
VERSION     V014967
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(50..154)
                     /label="AmpR promoter"
     promoter        500..518
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        631..974
                     /label="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     CDS             complement(1300..2412)
                     /gene="leu1"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from
                     Schizosaccharomyces pombe (strain 972 / ATCC 24843).
                     Accession#: P18869"
     terminator      2551..2748
                     /label="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2856..2874)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3132..3720)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(3894..4702,1..49))
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
       61 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
      121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
      181 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      241 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      301 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
      361 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
      421 tgagagtgca ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct
      481 tatgtatcat acacatacga tttaggtgac actatagaac gcggccgcca gctgaagctt
      541 cgtacgctgc aggtcgacgg atccccgggt taattaaggc gcgccagatc tgtttagctt
      601 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
      661 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
      721 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
      781 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
      841 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
      901 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
      961 gaacataaac aaccatggct agattatgtt acaggttact tcgtttaaaa agaaataatt
     1021 aatgatatta tgtttgaaac ttttcatagt aaaaaaagag taagtacaca gcgacaactc
     1081 ggtcataaag ttgaacggat gtcgtaaatc aattccatgc ttttgcaact taatacatct
     1141 ttgcttgact ataacatacc aaaagaaaaa acattagagc cttaaaattt acgcttttgt
     1201 caattgcatt tttaacgata ataaatatgc gacacaatta atcaataaaa attaggtcaa
     1261 atcaaatcat atgaatttat tctacattaa accctattac aaaatttttt caagttcttc
     1321 aacaacagcc ttagtaatat cagcggtaga agcctcacct cccaaatcgc gagtataaag
     1381 accacgtcca ccaattgaag tatcatccaa gaccttgcgt acggcggctt cgatagcttc
     1441 agcctcctta ggagcattca aaccataacg aaggagaagg gaagcagata aaattgtacc
     1501 aacaggatta acaatgccct tgccagcgat atcgggagcg ctaccgtgaa tgggctcaac
     1561 caaacaatga accttttctt ctgattttcc taccacaccg gaaagggagg cagaaggcaa
     1621 aaggcccaag ctaccaggaa tgacagaagc ctcatctgaa ataatgtcac caaacaagtt
     1681 gtcagtcaaa acaacaccgt taagtgtacg agggctcttg accaaaagca tggctgcgga
     1741 gtcaatgagc tggtttttta aggtaaggtg aggatattcc tccttaaaaa tcttagctac
     1801 agtcttgcgc caaagacgag aagttgccaa aacattagct ttgtcgagta atgtgacggg
     1861 agcaggaggg ttggaagttt cagctaacca agcagccaaa cgagcaatac gagaaacttc
     1921 ttccaaactg taaggccaag tgtccatagc ataacccgat ccgttgtcct cagtgcgctc
     1981 accaaagtaa caacctccag taagttctcg tacaacacaa aaatcgacac cttcaacgat
     2041 ttcaggcttc aaagggctgt acttgactaa agacttgctg gcaaagttgc aaggtcgaag
     2101 gttggcccaa acacccatac tcttacgaag cttcaataaa ccttgctcag gacgacaatt
     2161 ggggttggtc cattcaggac caccaacggc acccaaaaga acaccgtcag cttccaaaca
     2221 agccttcaca gtctcgtcag tcaaaggggt tccataggca tcaatagagg cacctccaat
     2281 cttgtgttct tcaaactcga gttttaactc aggtcgcttc ttctcaacga ctttcaaaac
     2341 ctccaaggca gaagcaacaa tttcagggcc aatatggtct cctggtaaga cgacgatttt
     2401 ctttgcacac atgttgttga agaagttttg ttgtgaaatg gtttcgtgaa agtttcagac
     2461 cctaccgcaa aaatgcctgg tttcgggaaa ctcaacactg ttgcactttt tatactacag
     2521 attgggatat cgatctagcc cctaatcagt actgacaata aaaagattct tgttttcaag
     2581 aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc
     2641 gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag
     2701 aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga
     2761 tactaacgcc gccatccagt gtcgaaaacg agctcgaatt catcgatgat atcagatcca
     2821 ctagtggcct atgcggccgc ggatctgccg gtctccctat agtgagtcgt attaatttcg
     2881 ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2941 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3001 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3061 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3121 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3181 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3241 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3301 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3361 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3421 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3481 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3541 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3601 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3661 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3721 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3781 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3841 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3901 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3961 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4021 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4081 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4141 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4201 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4261 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4321 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4381 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4441 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4501 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4561 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4621 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4681 tgagcaaaaa caggaaggca aa
//
