LOCUS       40924_7771       11797 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector PC-GW-Kan, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Dalal J, Yalamanchili R, La Hovary C, Ji M, Rodriguez-Welsh M, 
            Aslett D, Ganapathy S, Grunden A, Sederoff H, Qu R.
  TITLE     A novel gateway-compatible binary vector series (PC-GW) for flexible
            cloning of multiple genes for genetic transformation of plants
  JOURNAL   Plasmid 81, 55-62 (2015)
  PUBMED    26188330
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   Dalal J, Yalamanchili R, Hovary CL, Ji M, Rodriguez-Welsh M, Aslett 
            D, Ganapathy S, Grunden A, Sederoff H, Qu R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2015) Crop Science, North Carolina State 
            University, Campus Box 7287, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 11797)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11797)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 81,
            55-62 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-2015) Crop Science, North Carolina State University, Campus 
            Box 7287, Raleigh, NC 27695, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1219..1845
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2282..3346
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3415..3609
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3953..4093
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4279..4867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4957..5748)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6173..6197
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6275..6449)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6509..7297)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(7366..8043)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    8234..8255
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8270..8300
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8308..8324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8332..8348
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8965..9310
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    9399..9518
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9548..9578
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             9632..10288
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             10633..10935
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(11010..11134)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      11238..11463
                     /label=35S terminator from pSIM1 cloning vector
                     /note="35S terminator from pSIM1 cloning vector"
                     /regulatory_class="terminator"
     primer_bind     complement(11473..11489)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11692..11716
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
     4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg
     6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg
     6421 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc tagctagagg
     6481 atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     6541 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     6601 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     6661 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     6721 gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     6781 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     6841 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     6901 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     6961 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     7021 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     7081 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc agggcaccgg
     7141 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     7201 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     7261 cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat cgacagatct
     7321 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     7381 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg
     7441 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
     7501 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     7561 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     7621 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     7681 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     7741 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa
     7801 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     7861 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     7921 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     7981 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     8041 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     8101 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc
     8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8341 gctatgacca tgattacgaa ttccgtaact ataacggtcc taaggtagcg aacttgatat
     8401 ctatgcgtat ccgcctgatg aatgagttac gctagggata acagggtaat ataggaccat
     8461 attgactacg gctgcagaga ttagcctttt caatttcaga aagaatgcta acccacagat
     8521 ggttagagag gcttacgcag caggtctcat caagacgatc tacccgagca ataatctcca
     8581 ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg
     8641 catcaagaac acagagaaag atatatttct caagatcaga agtactattc cagtatggac
     8701 gattcaaggc ttgcttcaca aaccaaggca agtaatagag attggagtct ctaaaaaggt
     8761 agttcccact gaatcaaagg cgatggagtc aaagattcaa atagaggacc taacagaact
     8821 cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa
     8881 aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
     8941 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
     9001 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
     9061 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
     9121 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     9181 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     9241 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     9301 ggagagaaca cggctgcagg aaagacggtt ctagaatccg cttaagacct cctaggtcca
     9361 acgcgttttc tactagttac atttaaatat atagacgtcc aagtttgtac aaaaaagctg
     9421 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     9481 agactacata atactgtaaa acacaacata tccagtcata ttggcggccg cattaggcac
     9541 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttagtatgc
     9601 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     9661 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     9721 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     9781 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     9841 tccggtatcc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     9901 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     9961 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
    10021 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
    10081 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
    10141 tttcacgatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
    10201 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
    10261 gtactgcgat gagtggcagg gcggggcgta aacgcgtgca tccggcttac taaaagccag
    10321 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
    10381 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
    10441 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
    10501 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
    10561 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
    10621 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
    10681 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
    10741 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
    10801 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
    10861 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
    10921 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtcagc aggtcgacca
    10981 tagtgactgg atatgttgtg ttttacagcc atagtgactg gatatgttgt gttttacagt
    11041 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
    11101 acgtttctcg ttcagctttc ttgtacaaag tggtggatcc catatcatgt gtattggcaa
    11161 acagctatta tgggtattat gggtaattca ttggttcatt atctatgtcg ggtgcggaga
    11221 aagaggtaat gaaatggcgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca
    11281 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
    11341 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
    11401 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
    11461 cctaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    11521 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    11581 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt
    11641 gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat
    11701 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg
    11761 gcgtgaaaag gtttatccgt tcgtccattt gtatgtg
//
