LOCUS       40924_7766       12013 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector PC-GW-Hyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Dalal J, Yalamanchili R, La Hovary C, Ji M, Rodriguez-Welsh M, 
            Aslett D, Ganapathy S, Grunden A, Sederoff H, Qu R.
  TITLE     A novel gateway-compatible binary vector series (PC-GW) for flexible
            cloning of multiple genes for genetic transformation of plants
  JOURNAL   Plasmid 81, 55-62 (2015)
  PUBMED    26188330
REFERENCE   2  (bases 1 to 12013)
  AUTHORS   Dalal J, Yalamanchili R, Hovary CL, Ji M, Rodriguez-Welsh M, Aslett 
            D, Ganapathy S, Grunden A, Sederoff H, Qu R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2015) Crop Science, North Carolina State 
            University, Campus Box 7287, Raleigh, NC 27695, USA
REFERENCE   3  (bases 1 to 12013)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12013)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 81,
            55-62 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-2015) Crop Science, North Carolina State University, Campus 
            Box 7287, Raleigh, NC 27695, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        608..953
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1042..1161
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1191..1221
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1275..1931
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2276..2578
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2653..2777)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      2881..3106
                     /label=35S terminator from pSIM1 cloning vector
                     /note="35S terminator from pSIM1 cloning vector"
                     /regulatory_class="terminator"
     primer_bind     complement(3116..3132)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3335..3359
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4659..5285
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5722..6786
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6855..7049
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7393..7533
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7719..8307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8397..9188)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    9613..9637
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9715..9889)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9932..10954)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(11022..11699)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11890..11911
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11926..11956
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11964..11980
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11988..12004
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattccgta actataacgg tcctaaggta gcgaacttga tatctatgcg tatccgcctg
       61 atgaatgagt tacgctaggg ataacagggt aatataggac catattgact acggctgcag
      121 agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga gaggcttacg
      181 cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc aaataccttc
      241 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga
      301 aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
      361 acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc actgaatcaa
      421 aggcgatgga gtcaaagatt caaatagagg acctaacaga actcgccgta aagactggcg
      481 aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc gtcaacatgg
      541 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
      601 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
      661 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
      721 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
      781 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
      841 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
      901 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga acacggctgc
      961 aggaaagacg gttctagaat ccgcttaaga cctcctaggt ccaacgcgtt ttctactagt
     1021 tacatttaaa tatatagacg tccaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
     1081 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
     1141 aaaacacaac atatccagtc atattggcgg ccgcattagg caccccaggc tttacacttt
     1201 atgcttccgg ctcgtataat gtgtggattt tgagttagta tgcgtcgaga ttttcaggag
     1261 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     1321 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     1381 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     1441 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggta tcccgtatgg
     1501 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     1561 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     1621 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     1681 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     1741 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacg atgggcaaat
     1801 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     1861 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     1921 agggcggggc gtaaacgcgt gcatccggct tactaaaagc cagataacag tatgcgtatt
     1981 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     2041 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     2101 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     2161 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     2221 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     2281 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     2341 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     2401 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     2461 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     2521 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     2581 aatgtcaggc tcccttatac acagccagtc agcaggtcga ccatagtgac tggatatgtt
     2641 gtgttttaca gccatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     2701 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     2761 ttcttgtaca aagtggtgga tcccatatca tgtgtattgg caaacagcta ttatgggtat
     2821 tatgggtaat tcattggttc attatctatg tcgggtgcgg agaaagaggt aatgaaatgg
     2881 cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
     2941 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     3001 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     3061 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctaagc ttggcactgg
     3121 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3181 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3241 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
     3301 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     3361 taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc
     3421 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
     3481 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
     3541 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
     3601 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
     3661 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
     3721 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
     3781 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
     3841 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
     3901 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
     3961 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
     4021 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     4081 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
     4141 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
     4201 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
     4261 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
     4321 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
     4381 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
     4441 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     4501 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     4561 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     4621 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     4681 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     4741 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     4801 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     4861 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     4921 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     4981 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     5041 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     5101 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     5161 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     5221 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     5281 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     5341 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     5401 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     5461 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     5521 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     5581 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     5641 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
     5701 agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     5761 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     5821 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     5881 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
     5941 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
     6001 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
     6061 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
     6121 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
     6181 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
     6241 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
     6301 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
     6361 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
     6421 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
     6481 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
     6541 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
     6601 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
     6661 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
     6721 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
     6781 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
     6841 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
     6901 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
     6961 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
     7021 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
     7081 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
     7141 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
     7201 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
     7261 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     7321 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     7381 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     7441 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     7501 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     7561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac
     8401 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
     8461 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
     8521 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
     8581 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
     8641 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
     8701 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
     8761 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
     8821 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
     8881 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
     8941 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
     9001 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
     9061 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
     9121 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
     9181 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
     9241 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
     9301 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
     9361 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
     9421 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
     9481 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
     9541 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
     9601 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
     9661 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg
     9721 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
     9781 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg
     9841 aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt
     9901 cgatcgacag atccggtcgg catctactct atttctttgc cctcggacga gtgctggggc
     9961 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
    10021 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
    10081 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
    10141 caagaccaat gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc
    10201 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
    10261 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
    10321 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
    10381 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
    10441 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
    10501 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
    10561 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta
    10621 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
    10681 agatgcaata ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga
    10741 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
    10801 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
    10861 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
    10921 cgacagacgt cgcggtgagt tcaggctttt tcatatctca ttgccccccg ggatctgcga
    10981 aagctcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa
    11041 tgaaatgaac ttccttatat agaggaaggt cttgcgaagg atagtgggat tgtgcgtcat
    11101 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
    11161 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
    11221 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
    11281 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
    11341 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
    11401 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttatca catcaatcca
    11461 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
    11521 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
    11581 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
    11641 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
    11701 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
    11761 gctccaccat gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg
    11821 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    11881 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    11941 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    12001 tgaccatgat tac
//
