LOCUS       .                       8376 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1389..1600
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     LTR             1613..1881
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2702..3418
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pédelacq et al., 2006)"
     misc_feature    3431..4017
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4030..4821
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4946..5078
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6355..6371)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6379..6395)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6403..6433)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6448..6469)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6757..7345)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7519..8376)
                     /label="AmpR"
                     /note="β-lactamase"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccaagg atctgcgatc gctccggtgc
     1381 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     1441 caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     1501 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     1561 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg
     1621 catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg
     1681 cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt
     1741 aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag
     1801 ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc
     1861 tgttctgcgc cgttacagat ccaagctgtg accggcgcct actctagagc tagcgaattc
     1921 gcccttggcc atggccgagg tgaagctggt ggagtctggg ggaggcttag tgaagcctgg
     1981 agggtccctg aaactctcct gtgcagcctc tggattcact ttcagtagtt atgccatgtc
     2041 ttgggttcgc cagactccgg agaagaggct ggagtgggtc gcaaccatta gtagtggtgg
     2101 tagttatacc tactatccaa acactgtgaa ggggcgattc accatctcca gagacaatgc
     2161 caagaacacc ctgtacctgc aaatgagcag tctgaggtct gaggacacgg ccatatatta
     2221 ctgtgcaaga cacggggtac gacatagagt ggactacttt gactactggg gccaaggcac
     2281 cactctcaca gtctcctcag gtggaggcgg ttcaggcgga ggtggctctg gcggtggcgg
     2341 atcggacatt gtgctgaccc aatctccagc ttctttgact gtgtctctag ggcagagggc
     2401 caccatctcc tgcaaggcca gccaaagtgt tgattatgat ggtgatagtt atatgaactg
     2461 gtaccaacag aaaccaggac agccacccaa actcctcatc tatgctgcat ccaatctaga
     2521 atctgggatc ccagccaggt ttagtggcag tgggtctggg acagacttca ccctcaacat
     2581 ccatcctgtg gaggaggagg atgctgcaac ctattactgt cagcaaagta atgaggatcc
     2641 gctcacgttc ggtgctggga ccaagctgga aataaaacgg gcggatccac cggtcgccac
     2701 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2761 cggcgacgta aacggccaca agttcagcgt gcgcggcgag ggcgagggcg atgccaccaa
     2821 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2881 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2941 gcgccacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatcag
     3001 cttcaaggac gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3061 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3121 caagctggag tacaacttca acagccacaa cgtctatatc accgccgaca agcagaagaa
     3181 cggcatcaag gccaacttca agatccgcca caacgtggag gacggcagcg tgcagctcgc
     3241 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3301 ctacctgagc acccagtccg tgctgagcaa agaccccaac gagaagcgcg atcacatggt
     3361 cctgctggag ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaagta
     3421 agcggccgcg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     3481 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3541 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     3601 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     3661 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     3721 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     3781 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     3841 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     3901 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     3961 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     4021 gcgtccggaa tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4081 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4141 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4201 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccgcgacggg cgttccttgc
     4261 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4321 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     4381 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     4441 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     4501 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     4561 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     4621 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     4681 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     4741 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     4801 cgccttcttg acgagttctt ctgagtcgac aatcaacctc tggattacaa aatttgtgaa
     4861 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4921 atgcctttgt atcatgcgtt aactaaactt gtttattgca gcttataatg gttacaaata
     4981 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5041 tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt
     5101 agtctatcag aagctcatct ggtctccctt ccgggggaca agacatccct gtttaatatt
     5161 taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa ccaggttgca
     5221 gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc aggtttagcc
     5281 ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc ccgtatcccc
     5341 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc
     5401 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc
     5461 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat
     5521 tacatccctg ggggctttgg gggggggctg tccctgatat ctataacaag aaaatatata
     5581 tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa
     5641 atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt
     5701 tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga
     5761 ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt
     5821 tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc tagctgcatc
     5881 aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg
     5941 gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt
     6001 ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga
     6061 ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg
     6121 ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca
     6181 gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca
     6241 gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg atgccgcaga
     6301 aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat catggtcata
     6361 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6421 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6481 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8341 gggaataagg gcgacacgga aatgttgaat actcat
//
