LOCUS       40924_2348        4215 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector P1-EYFP-pA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK.
  TITLE     Multiplexed and programmable regulation of gene networks with an 
            integrated RNA and CRISPR/Cas toolkit in human cells
  JOURNAL   Mol. Cell 54 (4), 698-710 (2014)
  PUBMED    24837679
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Perli SD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2014) Electrical Engineering and Computer Science,
            Massachusetts Institute of Technology, 77, Massachusetts Avenue, 
            Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4215)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4215)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell";
            date: "2014"; volume: "54"; issue: "4"; pages: "698-710"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAY-2014) Electrical Engineering and Computer Science, 
            Massachusetts Institute of Technology, 77, Massachusetts Avenue, 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(471..1328)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1329..1433)
                     /label=AmpR promoter
     rep_origin      1460..1915
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    2046..2094
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    2108..2199
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     CDS             2649..3350
                     /codon_start=1
                     /label=PhiYFP
                     /note="Phialidium yellow fluorescent proteim"
                     /translation="MSSGALLFHGKIPYVVEMEGDVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEIAGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLTE
                     TVRAVDCRKTYL"
     polyA_signal    complement(3379..3500)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(join(3924..4215,1..297))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
       61 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      121 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
      181 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      241 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      301 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      361 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      421 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      481 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
      541 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
      601 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
      661 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
      721 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
      781 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      841 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      901 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
      961 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1021 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1081 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1141 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1201 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1261 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1321 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1381 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1441 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1501 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1561 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1621 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     1681 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     1741 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     1801 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     1861 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat
     1921 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1981 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg
     2041 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     2101 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
     2161 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgatagcta gctgaaatat
     2221 taatgtcgcg actattaata ttttcaggct agccatgctt cgctacacgc gactattaat
     2281 attttcaggc tagccatgct tcgctacacg cgactattaa tattttcaag ctagccatgc
     2341 ttcgctacac gcgactatta atattttcag gctagccatg cttcgctaac gcgactatta
     2401 atattttcag gctagtcatg cttcgctaca cgcgactatt aatattttca ggctagccat
     2461 gcttcgctac acgcgactat taatattttc aggctagcca tgcttcgcta cacgcgacta
     2521 ttaatatttt caggctagcc atgcttcgct acacgcgact attaatattt tcaggctagc
     2581 ggggggctat aaaagggggt gggggcgttc gtcctgctat ctagcgtcgc gttgaccatg
     2641 gcgccaccat gagcagcggc gccctgctgt tccacggcaa gatcccctac gtggtggaga
     2701 tggagggcga tgtggatggc cacaccttca gcatccgcgg taagggctac ggcgatgcca
     2761 gcgtgggcaa ggtggatgcc cagttcatct gcaccaccgg cgatgtgccc gtgccctgga
     2821 gcaccctggt gaccaccctg acctacggcg cccagtgctt cgccaagtac ggccccgagc
     2881 tgaaggattt ctacaagagc tgcatgcccg atggctacgt gcaggagcgc accatcacct
     2941 tcgagggcga tggcaatttc aagacccgcg ccgaggtgac cttcgagaat ggcagcgtgt
     3001 acaatcgcgt gaagctgaat ggccagggct tcaagaagga tggccacgtg ctgggcaaga
     3061 atctggagtt caatttcacc ccccactgcc tgtacatctg gggcgatcag gccaatcacg
     3121 gcctgaagag cgccttcaag atctgccacg agatcgccgg cagcaagggc gatttcatcg
     3181 tggccgatca cacccagatg aataccccca tcggcggcgg ccccgtgcac gtgcccgagt
     3241 accaccacat gagctaccac gtgaagctga gcaaggatgt gaccgatcac cgcgataata
     3301 tgagcctgac ggagaccgtg cgcgccgtgg attgccgcaa gacctacctg taaggccggc
     3361 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3421 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3481 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3541 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     3601 aggatcgagt atgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     3661 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     3721 taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3901 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4081 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     4141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4201 gaccgctgcg cctta
//
