LOCUS       40924_2328       10064 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector P-element_XP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Thibault ST, Singer MA, Miyazaki WY, Milash B, Dompe NA, Singh CM, 
            Buchholz R, Demsky M, Fawcett R, Francis-Lang HL, Ryner L, Cheung 
            LM, Chong A, Erickson C, Fisher WW, Greer K, Hartouni SR, Howie E, 
            Jakkula L, Joo D, Killpack K, Laufer A, Mazzotta J, Smith RD, 
            Stevens LM, Stuber C, Tan LR, Ventura R, Woo A, Zakrajsek I, Zhao L,
            Chen F, Swimmer C, Kopczynski C, Duyk G, Winberg ML, Margolis J.
  TITLE     A complementary transposon tool kit for Drosophila melanogaster 
            using P and piggyBac
  JOURNAL   Nat. Genet. 36 (3), 283-287 (2004)
  PUBMED    14981521
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Dompe NA, Buchholz R, Miyazaki WY, Kopczynski C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, 
            South San Francisco, CA 94083, USA
REFERENCE   3  (bases 1 to 10064)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10064)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Genet."; date: "2004"; volume: "36"; issue: "3"; pages: "283-287"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, South San 
            Francisco, CA 94083, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    597..644
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    1390..1437
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     gene            complement(1633..5769)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    7072..7304
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        8106..8210
                     /label=AmpR promoter
     CDS             8211..9068
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9242..9830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctcccgg gctgcagatc ttgcgacgtg aacagtgagc
      541 tgtatgtgcg cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag
      601 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaaaa
      661 ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc
      721 tagatctcga cgtgttcact ttgcttgttt gaattgaatt gtcgctccgt agacgaagcg
      781 cctctattta tactccggcg ctctctcggt accctcgacc cttgccggag gacagtactc
      841 cgccagcccg gaggacagta ctccgccttg cgtcggagga cagtactccg acctctagtg
      901 tcggaggaca gtactccgac cttgcgtcgg aggacagtac tccgacctct agtgtcggag
      961 gacagtactc cgaccttgcg tcggaggaca gtactccgac ctctagtgtc ggaggacagt
     1021 actccgacct tgccggagga cagtactccg cagcccggag gacagtactc cgccttgcgt
     1081 cggaggacag tactccgacc tctagtgtcg gaggacagta ctccgacctt gccggaggac
     1141 agtactccgc cagcccggag gacagtactc cgccttggag gcctaagctt gatgacctcg
     1201 agaaagagag agagaagaga agagagagaa cattcgagaa agagagagag aagagaagag
     1261 agagaacatt cgaggggggg ctagagcggc cgcccaattc gcccttgaag atcttgcgac
     1321 gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg
     1381 aaacgctttg aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcaga
     1441 gcgcttttga aaaccaaaag cgctctgaag acgcactttc aaaaaaccaa aaacgcaccg
     1501 gactgtaacg agctagatct tcaagggcga attgaggtcc tgttcattgt ttaatgaaaa
     1561 taagagcttg agggaaaaaa ttcgtacttt ggagtacgaa atgcgtcgtt tagagcagca
     1621 gccgaattaa ttctagttcc agtgaaatcc aagcattttc taaattaaat gtattcttat
     1681 tattatagtt gttatttttg atatatataa acaacactat tatgcccacc atttttttga
     1741 gatgcatcta cacaaggaac aaacactgga tgtcactttc agttcaaatt gtaacgctaa
     1801 tcactccgaa caggtcacaa aaaattacct taaaaagtca taatattaaa ttagaataaa
     1861 tatagctgtg agggaaatat atacaaatat attggagcaa ataaattgta catacaaata
     1921 tttattacta atttctattg agacgaaatg aaccactcgg aaccatttga gcgaaccgaa
     1981 tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg
     2041 agagcgggtg ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta
     2101 tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt
     2161 agcgataaac gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac
     2221 aaatccaaag tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat
     2281 atcttgtttt tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt
     2341 aaacacgatg gtaataatgg taaaaaaaaa aacaagcagt tatttcggat atatgtcggc
     2401 tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg
     2461 atgagaatgg ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc
     2521 tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg
     2581 cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat
     2641 gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg
     2701 ccaaagagca ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag
     2761 gagctggcgc aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc
     2821 agcgccaggc agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggataggca
     2881 atcgccgtga agaccagtgg cactgtgaga aaaagcggta attcggcaat cgttttgccc
     2941 agaaagtatg tgtcacagcg ataaagtcga cttcgggcct ccctcataaa aactggcagc
     3001 tctgaggtga acacctaaat cgaatcgatt cattagaaag ttagtaaatt attaatatgc
     3061 aaatgtattc taaacaagac ttacatttat cgtggcaaag acgttttgaa aggtcatgtt
     3121 ggtcaggaag aggaagatgg ctccgttgat attcatcacg cccacttgcg tgagttgttg
     3181 gcccaaaaag atgaggccaa tcaagatggc aaccatctgc aaattaaaat gttactcgca
     3241 tctcattaat attcatatct tcaacatgtt cgcgagttaa atgaaattta tttattttct
     3301 gcaaaactat aaactataca tctcattgaa aaaaactaag aagggtgtgg aatcaggcaa
     3361 ttctaactaa aatctagcga atttgtttcc aagaattgta agcgttatat catttgtttc
     3421 cactggaacc actcaccgtt gtctgaataa gtcgcacttt tacgaggagt ggttccttga
     3481 gcaccgacag ccaggatcgc cacaggaccg cccggaactg catgaaccag gtggccttgt
     3541 aggtgtaccc attctccggc tgctccagtg gcttctccaa atttttggtg gccaacaact
     3601 gctccatatc ccgggctact ttgctaatag caaaattgtc gcatatcttg gcgatccgat
     3661 cacgggactc gatctcccgt ccgggcacaa cggccaacac ctgtacgtaa aagtccgccg
     3721 gattgtagtt ggtaggacac tgggcaccca cgctggatag gagttgagat gtaatgtaat
     3781 gctagatacc cttaataaac acatcgaact cactaggaaa agaagtcgac ggcttcgctg
     3841 ggagtgccca agaaagctac cctgccctcg gccatcagaa ggatcttgtc aaagagctca
     3901 aacagctcgg aagacggctg atgaatggtc aggatgacgg tcttgccctt ctgcgacagc
     3961 ttcttcagca cctggacgac gctgtgggcg gtaaatgagt ccagtccgga ggtgggctca
     4021 tcgcagatca gaagcggcgg atcggttagt gcctcggagg cgaatgccag acgcttcctt
     4081 tctccgccgg acagaccttt caccctgccg ggcacaccga tgatcgtgtg ctgacatttg
     4141 ctgagcgaaa gctcctggat cacctgatcc acgcgggcca ctcgctgccg ataggtcaga
     4201 tgtcgtggca tccgcaccat ggcctggaaa atcaggtgtt ccctggccgt tagggagccg
     4261 ataaagaggt catcctgctg gacataggcg cacctggcct gcatctcctt ggcgtccaca
     4321 ggttggccat tgagcagtcg catcccggat ggcgatactt ggatgccctg cggcgatcga
     4381 aaggcaaggg cattcagcag ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa
     4441 agttcgcccg gataggccac gccgcaaact gagtttcaaa ttggtaattg gaccctttat
     4501 taagatttca cacagatcag ccgactgcga atagaaactc accgttcttg agcaaatgtt
     4561 tcctgggcgc cggtatgtgt cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct
     4621 gccgccatcc ggagcccggc tgattgaccg ccccaaagat gtccatattg tgccaggcat
     4681 aggtgaggtt ctcggctagt tggccgctcc ctgaaccgga gtcctccggc ggactgggtg
     4741 gcaggagcgt gccgtagttt ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag
     4801 ccgctccgct gtcaccctgc aatgataggg gatctcaaat atcaactaca agcgttatgc
     4861 tcatctaacc ccgaacaaaa cgaagtatcc tacgaagtag gtttatactt ttatttattt
     4921 tttgtgcatc taggatcagc ttaaaatatc tggttgttat attttttgta aaaaagaatg
     4981 tagtcgaaaa tgaatgcctt tagatgtctt gatcatgata tgatcttaaa aattgtctta
     5041 tatagcgagc acagctacca gaataatctg tttcgtgtca ctatttgttt gtgcgattgc
     5101 ggtttgggat ttttgtgggt cgcagttctc acgccgcaga caatttgatg ttgcaatcgc
     5161 agttcctata gatcaagtga acttaagatg tatgcacatg tactactcac attgttcaga
     5221 tgctcggcag atgggtgttt gctgcctccg cgaattaata gctcctgatc ctcttggccc
     5281 attgccggga tttttcacac tttcccctgc ttacccaccc aaaaccaatc accaccccaa
     5341 tcactcaaaa aacaaacaaa aataagaagc gagaggagtt ttggcacagc actttgtgtt
     5401 taattgatgg cgtaaaccgc ttggagcttc gtcacgaaac cgctgacaaa gtgcaactga
     5461 aggcggacat tgacgctagg taacgctaca aacggtggcg aaagagatag cggacgcagc
     5521 ggcgaaagag acggcgatat ttctgtggac agagaaggag gcaaacagcg ctgactttga
     5581 gtggaatgtc attttgagtg agaggtaatc gaaagaacct ggtacttcaa atacccttgg
     5641 atcgaagtaa atttaaaact gatcagataa gttcaatgat gtccagtgca gtaaaaaata
     5701 aaaaaaaaat atgttttttt aaatctacat tctccaaaaa agggttttat taacttacat
     5761 acatactaga attcggcttg ggtcgaccgc taggtaacgc tacaaacggt ggcgaaagag
     5821 atagcggacg cagcggcgaa agagacggcg atatttctgt ggacagagaa ggaggcaaac
     5881 agcgctgact ttgagtggaa tgtcattttg agtgagaggt aatcgaaaga acctggtaca
     5941 tcaaataccc ttggatcgaa gtaaatttaa aactgatcag ataagttcaa tgatatccag
     6001 tgcagtaaaa aaaaaaaatg ttttttttat ctactttccg caaaaatggg ttttattaac
     6061 ttacatacat actagaatta attcctcgac tatggcaaga aaaagtatgc aatataataa
     6121 tcttttattg ggtatgcaac gaaaatttgt ttcgtcaaag tatgcaatat tttttattaa
     6181 aagagggtat gcaatgtatt ttattaaaaa cgggtatgca atacaataat cttttattgg
     6241 gtastgcaac gaaaatttgt ttcgtcaaag tatgcaatat tttttattaa aagagggtat
     6301 gcaatgtatt ttattaaaaa cgggtatgca ataaaaaatt atttggtttc tctaaaaagt
     6361 atgcagcact tattttttga taaggtatgc gacaaaattt tactttgccg aaaatatgca
     6421 atgtttttgc gaataaattg tcgagcccag taagccgaat tcggtacccg cccgggatca
     6481 gatccgcggc cgctctagcc ccccctcgaa tgttctctct cttctcttct ctctctcttt
     6541 ctcgaatgtt ctctctcttc tcttctctct ctctttctcg aggtcatcaa gcttaggcct
     6601 ccaaggcgga gtactgtcct ccgggctggc ggagtactgt cctccggcaa ggtcggagta
     6661 ctgtcctccg acactagagg tcggagtact gtcctccgac gcaaggcgga gtactgtcct
     6721 ccgggctgcg gagtactgtc ctccggcaag gtcggagtac tgtcctccga cactagaggt
     6781 cggagtactg tcctccgacg caaggtcgga gtactgtcct ccgacactag aggtcggagt
     6841 actgtcctcc gacgcaaggt cggagtactg tcctccgaca ctagaggtcg gagtactgtc
     6901 ctccgacgca aggcggagta ctgtcctccg ggctggcgga gtactgtcct ccggcaaggg
     6961 tcgagggtac cgagagagcg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     7021 ttcaattcaa acaagcaaag tgaacacgtc gagatctctg cagccaagct ttgcgtactc
     7081 gcaaattatt aaaaataaaa ctttaaaaat aatttcgtct aattaatatt atgagttaat
     7141 tcaaacccca cggacatgct aagggttaat caacaatcat atcgctgtct cactcagact
     7201 caatacgaca ctcagaatac tattcctttc actcgcactt attgcaagca tacgttaagt
     7261 ggatgtctct tgccgacggg accaccttat gttatttcat catggtctgg ccattctcat
     7321 cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta
     7381 gccgacatat atccgaaata actgcttgtt ttttttttta ccattattac catcgtgttt
     7441 actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat
     7501 atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt
     7561 gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct
     7621 acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca
     7681 taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct
     7741 ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga
     7801 ttcggtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     7861 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7921 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7981 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     8041 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     8101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8281 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8341 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8401 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     8461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8521 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8581 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8641 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8761 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9061 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9361 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     9421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9601 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     9661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccttct
     9841 tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc
     9901 gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta
     9961 gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta
    10021 acttctccgc cgccgatctg ccgctggact acgtgggtct ggcc
//
