LOCUS       62056_1815        4261 bp DNA     circular SYN 10-SEP-2020
DEFINITION  Cloning vector Orbs(prox)_Reporter, complete sequence.
ACCESSION   MT127269
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Engineered systems of inducible anti-repressors for the next 
            generation of biological programming
  JOURNAL   Nat Commun 11 (1), 4440 (2020)
  PUBMED    32895374
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4261)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-FEB-2020) Chemical "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..80
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     regulatory      84..106
                     /label=operator Orbs
                     /note="operator Orbs"
                     /regulatory_class="other"
     regulatory      124..135
                     /label=strong bacterial RBS
                     /note="strong bacterial RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             142..855
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     terminator      870..956
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1497..2444)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(2492..2714)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3206..3300)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3334..4125)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtccaac atatggcacc ggaggctttc gtcttcacct cgagaattgt ttgacaatta
       61 atcatccggc tcgtataatg tgtggtcagc gaaacgtttc gctgactgag cacagaattc
      121 attaaagagg agaaaggtac catgagcaaa ggagaagaac ttttcactgg agttgtccca
      181 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtccg tggagagggt
      241 gaaggtgatg ctacaaacgg aaagcttacc cttaaattta tttgcactac tggaaaacta
      301 cctgttccat ggccaacact tgtcactact ctcacttatg gtgttcaatg cttttcccgt
      361 tatccggatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      421 caggaacgca ctatatcttt caaagatgac gggacttaca agacgcgtgc tgaagtcaag
      481 tttgaaggtg atacccttgt taatcgtatc gagttaaaag gtattgattt taaagaagat
      541 ggaaacattc tcggacacaa acttgagtac aactttaact cacacaatgt atacatcacg
      601 gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa cgttgaagat
      661 ggatccgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      721 cttttaccag acaaccatta cctgtcgaca caatctgtcc tttcgaaaga tcccaacgaa
      781 aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac acatggcatg
      841 gatgagctct acaaataatc tagaggcatc aaataaaacg aaaggctcag tcgaaagact
      901 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
      961 cgccctagac ctagggtacg ggttttgctg cccgcaaacg ggctgttctg gtgttgctag
     1021 tttgttatca gaatcgcaga tccggcttca gccggtttgc cggctgaaag cgctatttct
     1081 tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca
     1141 gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg
     1201 taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac
     1261 ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa
     1321 cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
     1381 tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca gttgttctac
     1441 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
     1501 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
     1561 aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc ctcaaaactg
     1621 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg
     1681 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
     1741 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
     1801 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
     1861 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
     1921 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
     1981 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
     2041 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
     2101 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     2161 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     2221 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
     2281 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     2341 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     2401 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     2461 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt
     2521 ttcccgggag atctgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     2581 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     2641 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     2701 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     2761 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     2821 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     2881 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     2941 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     3001 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     3061 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     3121 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     3181 ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     3241 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     3301 caagcgagct ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     3361 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     3421 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     3481 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     3541 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc
     3601 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     3661 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     3721 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     3781 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     3841 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     3901 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc
     3961 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     4021 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     4081 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     4141 tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     4201 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     4261 c
//
