LOCUS       62056_1805        4235 bp DNA     circular SYN 10-SEP-2020
DEFINITION  Cloning vector Ogac(core)_Reporter, complete sequence.
ACCESSION   MT127267
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Engineered systems of inducible anti-repressors for the next 
            generation of biological programming
  JOURNAL   Nat Commun 11 (1), 4440 (2020)
  PUBMED    32895374
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4235)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-FEB-2020) Chemical "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      51..79
                     /label=trc promoter
                     /note="trc promoter"
                     /regulatory_class="promoter"
     regulatory      51..56
                     /label=trc promoter -35 hexamer
                     /note="trc promoter -35 hexamer"
                     /regulatory_class="promoter"
     regulatory      57..73
                     /label=operator Ogac
                     /note="operator Ogac"
                     /regulatory_class="other"
     regulatory      74..79
                     /label=trc promoter -10 hexamer
                     /note="trc promoter -10 hexamer"
                     /regulatory_class="promoter"
     regulatory      98..109
                     /label=strong bacterial RBS
                     /note="strong bacterial RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             116..829
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     terminator      844..930
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1471..2418)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(2466..2688)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3180..3274)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3308..4099)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtccaac atatggcacc ggaggctttc gtcttcacct cgagaattgt ttgacattga
       61 cagcgctgtc aattataatc tgagcacaga attcattaaa gaggagaaag gtaccatgag
      121 caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      181 taatgggcac aaattttctg tccgtggaga gggtgaaggt gatgctacaa acggaaagct
      241 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      301 tactctcact tatggtgttc aatgcttttc ccgttatccg gatcatatga aacggcatga
      361 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga
      421 tgacgggact tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg
      481 tatcgagtta aaaggtattg attttaaaga agatggaaac attctcggac acaaacttga
      541 gtacaacttt aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa
      601 agctaacttc aaaattcgcc acaacgttga agatggatcc gttcaactag cagaccatta
      661 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
      721 gacacaatct gtcctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga
      781 gtttgtaact gctgctggga ttacacatgg catggatgag ctctacaaat aatctagagg
      841 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      901 tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctaggg tacgggtttt
      961 gctgcccgca aacgggctgt tctggtgttg ctagtttgtt atcagaatcg cagatccggc
     1021 ttcagccggt ttgccggctg aaagcgctat ttcttccaga attgccatga ttttttcccc
     1081 acgggaggcg tcactggctc ccgtgttgtc ggcagctttg attcgataag cagcatcgcc
     1141 tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa gttgtctcag gtgttcaatt
     1201 tcatgttcta gttgctttgt tttactggtt tcacctgttc tattaggtgt tacatgctgt
     1261 tcatctgtta cattgtcgat ctgttcatgg tgaacagctt taaatgcacc aaaaactcgt
     1321 aaaagctctg atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt
     1381 ccctttgata tctaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca
     1441 ctgatagata caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc
     1501 tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catgcttact
     1561 ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc
     1621 atcgtgtagt gtttttctta gtccgttacg taggtaggaa tctgatgtaa tggttgttgg
     1681 tattttgtca ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg
     1741 tctatctagt tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac
     1801 caatttcata ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt
     1861 aagcctttta aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag
     1921 gctaatctct atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc
     1981 ataaatcctc atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat
     2041 gaattttttt aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt
     2101 ttcgcttgag aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg
     2161 attcctgatt tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata
     2221 agcattttcc ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt
     2281 ccgttctttc cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat
     2341 tagcttggtt tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa
     2401 aacaactaat tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt
     2461 gagatgggct agtcaatgat aattactagt ccttttcccg ggagatctgg gtatctgtaa
     2521 attctgctag acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc
     2581 tagacctttg tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa
     2641 agaataaaaa aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc
     2701 agttccgcag tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc
     2761 ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc
     2821 ttttgtctcc gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg
     2881 cgctcacggc tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc
     2941 atgcaaggaa actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta
     3001 tggcgggtct gctatgtggt gctatctgac tttttgctgt tcagcagttc ctgccctctg
     3061 attttccagt ctgaccactt cggattatcc cgtgacaggt cattcagact ggctaatgca
     3121 cccagtaagg cagcggtatc atcaacaggc ttacccgtct tactgtccct agtgcttgga
     3181 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     3241 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctctcgaa ccccagagtc
     3301 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     3361 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     3421 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     3481 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     3541 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
     3601 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     3661 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     3721 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     3781 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     3841 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     3901 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     3961 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     4021 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     4081 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
     4141 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
     4201 caaccttacc agagggcgcc ccagctggca attcc
//
