LOCUS       Exported               10638 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector OX3441, complete sequence.
ACCESSION   JQ352759
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Martins S, Naish N, Walker AS, Morrison NI, Scaife S, Fu G, 
            Dafa'alla T, Alphey L.
  TITLE     Germline transformation of the diamondback moth, Plutella xylostella
            L., using the piggyBac transposable element
  JOURNAL   Insect Mol. Biol. 21 (4), 414-421 (2012)
  PUBMED    22621377
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Martins S, Naish N, Walker A, Morrison N, Scaife S, Fu G, Dafa'alla 
            T, Alphey L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park, 
            Abingdon, Oxon OX14 4RX, UK
REFERENCE   3  (bases 1 to 10638)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Cloning vector OX3441"
                     /mol_type="other DNA"
                     /db_xref="taxon:1176401"
     mobile_element  1..675
                     /mobile_element_type="transposon:piggyBac 5'"
     protein_bind    2597..2615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2618..2636
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2639..2657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2660..2678
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2681..2699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2702..2720
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2723..2741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2744..2762
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2765..2783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2786..2804
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2807..2825
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2828..2846
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2849..2867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2870..2888
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      2965..4166
                     /regulatory_class="promoter"
                     /note="Hr5ie1"
     enhancer        2985..3467
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     intron          4181..4243
                     /note="D. melanogaster scraps"
     regulatory      4262..4271
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    4277..4303
                     /label=nuclear localization signal
                     /note="nuclear localization signal"
     regulatory      4310..4319
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    4316..4990
                     /note="DsRed2; red fluorescent protein, variant of DsRed, 
                     from Discosoma sp."
     CDS             4316..4990
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     misc_feature    4996..5032
                     /label=nuclear localization signal
                     /note="nuclear localization signal"
     CDS             5000..5020
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5055..5282
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40"
     polyA_signal    5161..5282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5349..5448
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     mobile_element  6087..7135
                     /mobile_element_type="transposon:piggyBac 3'"
     primer_bind     complement(7836..7852)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8326..8430
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8431..9291
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9462..10050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    10338..10359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10374..10404
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10412..10428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10436..10452
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaccctagaa agatagtctg cgtaaaattg acgcatgcat tcttgaaata ttgctctctc
       61 tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc
      121 tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga ttttgaacta taacgaccgc
      181 gtgagtcaaa atgacgcatg attatctttt acgtgacttt taagatttaa ctcatacgat
      241 aattatattg ttatttcatg ttctacttac gtgataactt attatatata tattttcttg
      301 ttatagatat cgtgactaat atataataaa atgggtagtt ctttagacga tgagcatatc
      361 ctctctgctc ttctgcaaag cgatgacgag cttgttggtg aggattctga cagtgaaata
      421 tcagatcacg taagtgaaga tgacgtccag agcgatacag aagaagcgtt tatagatgag
      481 gtacatgaag tgcagccaac gtcaagcggt agtgaaatat tagacgaaca aaatgttatt
      541 gaacaaccag gttcttcatt ggcttctaac agaatcttga ccttgccaca gaggactatt
      601 agaggtaaga ataaacattg ttggtcaact tcaaagtcca cgaggcgtag ccgagtctct
      661 gcactgaaca ttgtcagatc ggcccggcgg agtggacacg ctagaccaaa tgtgttctgt
      721 gatgacctgc agtaggaaga cgaataggtg gcctatggca ttattgtacg gaatgataaa
      781 cattgcctgc ataaattctt ttattatata cagccataat gtcagtagca agggagaaaa
      841 ggtccaaagt cgcaaaaaat ttatgagaaa cctttacatg agcctgacgt catcgtttat
      901 gcgtaagcgt ttagaagctc ctactttgaa gagatatttg cgcgataata tctctaatat
      961 tttgccaaat gaagtgcctg gtacatcaga tgacagtact gaagagccag taatgaaaaa
     1021 acgtacttac tgtacttact gcccctctaa aataaggcga aaggcaaatg catcgtgcaa
     1081 aaaatgcaaa aaagttattt gtcgagagca taatattgat atgtgccaaa gttgtttctg
     1141 actgactaat aagtataatt tgtttctatt atgtataagt taagctaatt acttatttta
     1201 taatacaaca tgactgtttt taaagtacaa aataagttta tttttgtaaa agagagaatg
     1261 tttaaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt ttaataaata
     1321 aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata
     1381 aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat
     1441 gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat ctttctaggg
     1501 ttaaaatgaa tgtaagcact ttattaacga aatctttggg aatatttcgc tcatcagcat
     1561 tttatttgag caggagtccg agatgcccgg gcggccgttt ttcttgaaat attgctctct
     1621 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
     1681 ctcccaaacg cgccagtggt agtacacagt actgtgggtg ttcagtttga aatcctcttg
     1741 cttctccatt gtctcggtta cctttggtca aatccatggg ttctattgcc tatatactct
     1801 tgcgattacc agtgattgcg ctattagcta ttagatggat tgttggccaa acttgtcgct
     1861 taagtggctg ggaattgtaa ccgtaggccc gagtgtaatg atcccccata aaaagttttc
     1921 gcaatgcctt tattttttgt tgcaaatctc tctttattct gcggtattct tcattattgc
     1981 ggggatgggg aaagtgttta tatagaagca acttacgatt gaacccaaat gcacctgaca
     2041 agcaaggtca aagggccaga tttttaaata tattatttag tcttaggact ctctatttgc
     2101 aattaaatta ctttgctacc tgagggttaa atcttcccca ttgataataa taattccact
     2161 atatgttcaa ttgggtttca ccgcgcttag ttacatgacg agccctaatg agccgtcggt
     2221 ggtctataaa ctgtgcctta caaatacttg caactcttct cgttttgaag tcagcagagt
     2281 tattgctaat tgctaattgc taattgcttt taactgattt cttcgaaatt ggtgctatgt
     2341 ttatggcgct attaacaagt atgaatgtca ggtttaacca ggggatgctt aattgtgttc
     2401 tcaacttcaa aggcagaaat gtttactctt gaccatgggt ttaggtataa tgttatcaag
     2461 ctcctcgagt taacgttacg ttaacgttaa cgttcgaggt cgactctagc gcgccccggc
     2521 catcgagaaa gagagagaga agagaagaga gagaacattc gagaaagaga gagagaagag
     2581 aagagagaga acatactccc tatcagtgat agagaagtcc ctatcagtga tagagatgtc
     2641 cctatcagtg atagagagtt ccctatcagt gatagagacg tccctatcag tgatagagaa
     2701 gtccctatca gtgatagaga gatccctatc agtgatagag atttccctat cagtgataga
     2761 gaggtcccta tcagtgatag agacttccct atcagtgata gagaaatccc tatcagtgat
     2821 agagacatcc ctatcagtga tagagaactc cctatcagtg atagagacct ccctatcagt
     2881 gatagagatc gatgcggccg catggtaccc attgcttgtc atttattaat ttggatgatg
     2941 tcatttgttt ttaaaattga actggcttta cgagtagaat tctacgcgta aaacacaatc
     3001 aagtatgagt cataatctga tgtcatgttt tgtacacggc tcataaccga actggcttta
     3061 cgagtagaat tctacttgta atgcacgatc agtggatgat gtcatttgtt tttcaaatcg
     3121 agatgatgtc atgttttgca cacggctcat aaactcgctt tacgagtaga attctacgtg
     3181 taacgcacga tcgattgatg agtcatttgt tttgcaatat gatatcatac aatatgactc
     3241 atttgttttt caaaaccgaa cttgatttac gggtagaatt ctacttgtaa agcacaatca
     3301 aaaagatgat gtcatttgtt tttcaaaact gaactcgctt tacgagtaga attctacgtg
     3361 taaaacacaa tcaagaaatg atgtcatttg ttataaaaat aaaagctgat gtcatgtttt
     3421 gcacatggct cataactaaa ctcgctttac gggtagaatt ctacgcgtaa aacatgattg
     3481 ataattaaat aattcatttg caagctatac gttaaatcaa acggacgctc gaggttgcac
     3541 aacactatta tcgatttgca gttcgggaca taaatgttta aatatatcga tgtctttgtg
     3601 atgcgcgcga catttttgta ggttattgat aaaatgaacg gatacgttgc ccgacattat
     3661 cattaaatcc ttggcgtaga atttgtcggg tccattgtcc gtgtgcgcta gtagcatgcc
     3721 cgtaacggac ctcgtacttt tggcttcaaa ggttttgcgc acagacaaaa tgtgccacac
     3781 ttgcagctct gcatgtgtgc gcgttaccac aaatcccaac ggcgcagtgt acttgttgta
     3841 tgcaaataaa tctcgataaa ggcgcggcgc gcgaatgcag ctgatcacgt acgctcctcg
     3901 tgttccgttc aaggacggtg ttatcgacct cagattaatg tttatcggcc gactgttttc
     3961 gtatccgctc accaaacgcg tttttgcatt aacattgtat gtcggcggat gttctatatc
     4021 taatttgaat aaataaacga taaccgcgtt ggttttagag ggcataataa aagaaatatt
     4081 gttatcgtgt tcgccattag ggcagtataa attgacgttc atgttggata ttgtttcagt
     4141 tgcaagttga cactggcggc gacaagcaat tctaattggg gtaagttttc ccgttctttt
     4201 ctgggttctt cccttttgct catccttgct gcactacctt caggtgcaag ttgagattca
     4261 ggccaccatg ggagatccca ccccacccaa gaagaagcgc aaaccggtcg ccaccatggc
     4321 ctcctccgag aacgtcatca ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt
     4381 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggccacaa
     4441 caccgtgaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     4501 cccccagttc cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta
     4561 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     4621 cggcgtggcg accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt
     4681 gaagttcatc ggcgtgaact tcccctccga cggccccgtg atgcagaaga agaccatggg
     4741 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagaccca
     4801 caaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat
     4861 ggccaagaag cccgtgcagc tgcccggcta ctactacgtg gacgccaagc tggacatcac
     4921 ctcccacaac gaggactaca ccatcgtgga gcagtacgag cgcaccgagg gccgccacca
     4981 cctgttcctg agatctcgac ccaagaaaaa gcggaaggtg gaggacccgt aagatccacc
     5041 ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     5101 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     5161 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5221 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5281 taacgcgagt tatcgcgctc gcgcgactga cggtcgtaag cacccgcgta cgtgtccacc
     5341 ccggtcacaa ccccttgtgt catgtcggcg accctacgcc cccaactgag agaactcaaa
     5401 ggttacccca gttggggcac tactcccgaa aaccgcttct gacctgggaa aacgtgaagc
     5461 cccggggcat ccgctgaggg ttgccgccgg ggcttcggtg tgtccgtcag tacttaatta
     5521 acaccgaaat cgtaattcac ggcatcatta caaaatattt tgacgttttg gacctcgtcc
     5581 ctaatgacac cataacggtg gccttgaagt atatttaacc ctagaaagat agtctgcgta
     5641 aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg
     5701 ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg
     5761 cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta
     5821 tcttttacgt gacttttaag atttaactca tacgataatt atattgttat ttcatgttct
     5881 acttacgtga taacttatta tatatatatt ttcttgttat agatatcgtg actaatatat
     5941 aataaaatgg gtagttcttt agacgatgag catatcctct ctgctcttct gcaaagcgat
     6001 gacgagcttg ttggtgagga ttctgacagt gaaatatcag atcacgtaag tgaagatgac
     6061 gtccaggaaa tctggccggc cgcaaccatt gtgggaaccg tgcgatcaaa caaacgcgag
     6121 ataccggaag tactgaaaaa cagtcgctcc aggccagtgg gaacatcgat gttttgtttt
     6181 gacggacccc ttactctcgt ctcatataaa ccgaagccag ctaagatggt atacttatta
     6241 tcatcttgtg atgaggatgc ttctatcaac gaaagtaccg gtaaaccgca aatggttatg
     6301 tattataatc aaactaaagg cggagtggac acgctagacc aaatgtgttc tgtgatgacc
     6361 tgcagtagga agacgaatag gtggcctatg gcattattgt acggaatgat aaacattgcc
     6421 tgcataaatt cttttattat atacagccat aatgtcagta gcaagggaga aaaggtccaa
     6481 agtcgcaaaa aatttatgag aaacctttac atgagcctga cgtcatcgtt tatgcgtaag
     6541 cgtttagaag ctcctacttt gaagagatat ttgcgcgata atatctctaa tattttgcca
     6601 aatgaagtgc ctggtacatc agatgacagt actgaagagc cagtaatgaa aaaacgtact
     6661 tactgtactt actgcccctc taaaataagg cgaaaggcaa atgcatcgtg caaaaaatgc
     6721 aaaaaagtta tttgtcgaga gcataatatt gatatgtgcc aaagttgttt ctgactgact
     6781 aataagtata atttgtttct attatgtata agttaagcta attacttatt ttataataca
     6841 acatgactgt ttttaaagta caaaataagt ttatttttgt aaaagagaga atgtttaaaa
     6901 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
     6961 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
     7021 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
     7081 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaaata
     7141 atagtttcta atttttttat tattcagcct gctgtcgtga ataccgtata tctcaacgct
     7201 gtctgtgaga ttgtcgtatt ctagcctttt tagtttttcg ctcatcgact tgatattgtc
     7261 cgacacattt tcgtcgattt gcgttttgat caaagacttg agcagagaca cgttaatcaa
     7321 ctgttcaaat tgatccatat taacgatatc aacccgatgc gtatatggtg cgtaaaatat
     7381 attttttaac cctcttatac tttgcactct gcgttaatac gcgttcgtgt acagacgtaa
     7441 tcatgttttc ttttttggat aaaactccta ctgagtttga cctcatatta gaccctcaca
     7501 agttgcaaaa cgtggcattt tttaccaatg aagaatttaa agttatttta aaaaatttca
     7561 tcacagattt aaagaagaac caaaaattaa attatttcaa cagtttaatc gaccagttaa
     7621 tcaacgtgta cacagacgcg tcggcaaaaa acacgcagcc cgacgtgttg gctaaaatta
     7681 ttaaatcaac ttgtgttata gtcacggatt tgccgtccaa cgtgttcctc aaaaagttga
     7741 agaccaacaa gtttacggac actattaatt atttgatttt gccccacttc attttgtggg
     7801 atcacaattt tgttatattt taaacaaagc ttggcactgg ccgtcgtttt acaacgtcgt
     7861 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     7921 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     7981 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     8041 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     8101 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     8161 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     8221 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     8281 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8341 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8401 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8461 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8521 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8581 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8641 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8701 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8761 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8821 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8881 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8941 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9001 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9061 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9121 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9181 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9241 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9301 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9361 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9421 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9481 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9541 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9601 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9661 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9721 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9781 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9841 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9901 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9961 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10021 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10081 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10141 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10201 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    10261 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    10321 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    10381 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    10441 aacagctatg accatgatta cgaatttcga cctgcaggca tgcaagcttg catgcctgca
    10501 ggtcgacgct cgcgcgactt ggtttgccat tctttagcgc gcgtcgcgtc acacagcttg
    10561 gccacaatgt ggtttttgtc aaacgaagat tctatgacgt gtttaaagtt taggtcgagt
    10621 aaagcgcaaa tctttttt
//
