LOCUS       40924_2229       12849 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary expression vector NLS-YC3.6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     FRET-based genetically encoded sensors allow high-resolution live 
            cell imaging of Ca(2) dynamics
  JOURNAL   Plant J. 69 (1), 181-192 (2012)
  PUBMED    21910770
REFERENCE   2  (bases 1 to 12849)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2011) Department of Developmental Biology, Centre 
            for Organismal Studies Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 12849)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12849)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2012"; volume: "69"; issue: "1"; pages: "181-192"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2011) Department of Developmental Biology, Centre for 
            Organismal Studies Heidelberg, Im Neuenheimer Feld 230, Heidelberg 
            69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    303..324
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        339..369
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    377..393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     401..417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      444..1089
                     /label=AtUBQ10
                     /note="AtUBQ10"
                     /regulatory_class="promoter"
     CDS             1124..1144
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1175..1858
                     /label=CFP
                     /note="cyan variant of GFP"
     CDS             2315..2392
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             2615..3133
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     terminator      3152..3793
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3806..3822)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(4404..4656)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4855..5646)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5676..6056)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    complement(6428..6452)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6980..7768
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      8015..8603
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8789..8929)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9273..9467)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9536..10600)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11037..11663)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      241 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      301 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      361 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      421 gattacgaat tcgagctcgg tacccgacga gtcagtaata aacggcgtca aagtggttgc
      481 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
      541 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
      601 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
      661 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
      721 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
      781 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
      841 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
      901 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
      961 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gatctggtgt
     1021 tagtttctag tttgtgcgat cgaatttgta gattaatctg agtttttctg attaacagct
     1081 cgagtgcggg atcctctaga gggcccggga cgtcatgctg cagcctaaga agaagagaaa
     1141 ggttggagga gtcgactcga gtgcggccgc caccatggtg agcaagggcg aggagctgtt
     1201 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaggttcag
     1261 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1321 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt
     1381 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1441 gcccgaaggc tacgtccagg agcgtaccat cttcttcaag gacgacggca actacaagac
     1501 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1561 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca
     1621 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggcccact tcaagatccg
     1681 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1741 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1801 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgcccg
     1861 catgcatgac caactgacag aagagcagat tgcagagttc aaagaagcct tctcattatt
     1921 cgacaaggat ggggacggca ccatcaccac aaaggaactt ggcaccgtta tgaggtcgct
     1981 tggacaaaac ccaacggaag cagaattgca ggatatgatc aatgaagtcg atgctgatgg
     2041 caatggaacg atttactttc ctgaatttct tactatgatg gctagaaaaa tgaaggacac
     2101 agacagcgaa gaggaaatcc gagaagcatt ccgtgttttt gacaaggatg ggaacggcta
     2161 catcagcgct gctcagttac gtcacgtcat gacaaacctc ggggagaagt taacagatga
     2221 agaagttgat gaaatgataa gggaagcaga tatcgatggt gatggccaag taaactatga
     2281 agagtttgta caaatgatga cagcaaaggg ggggaagagg cgctggaaga aaaacttcat
     2341 tgccgtcagc gctgccaacc ggttcaagaa gatctccagc tccggggcac tggagctcat
     2401 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2461 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     2521 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2581 catggacgag ctgtacaagg gtggcagcgg tggcatggtg agcaagggcg aggagctgtt
     2641 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     2701 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
     2761 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
     2821 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2881 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2941 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3001 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     3061 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     3121 ccacaacatc gagtaaacta gttggctgca gagctttcgt tcgtatcatc ggtttcgaca
     3181 acgttcgtca agttcaatgc atcagtttca ttgcgcacac accagaatcc tactgagttc
     3241 gagtattatg gcattgggaa acatgttttt cttgtaccat ttgttgtgct tgtaatttac
     3301 tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt
     3361 aatgaatgat atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat
     3421 ttgttgtgtg ttgaatttga aaatataaga gatatgcaaa cattttgttt tgagtaaaaa
     3481 tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga ggagtaaaac acttgtagtt
     3541 gtaccattat gcttattcac taggcaacaa atatattttc agacctagaa aagctgcaaa
     3601 tgttactgaa tacaagtatg tcctcttgtg ttttagacat ttatgaactt tcctttatgt
     3661 aattttccag aatccttgtc agattctaat cattgcttta taattatagt tatactcatg
     3721 gatttgtagt tgagtatgaa aatatttttt aatgcatttt atgacttgcc aattgattga
     3781 caacatgcat caatcgaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3841 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3901 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3961 gctagagcag cttgccaatc gaggccgacc aaccgcaagc gttgtcagtg ttgcaaagcg
     4021 ctctgtgtgg gcctacttta attgcttcca gtgttaaatt ggcgaaaggc aataatatcg
     4081 caaaatattg tgttgtaaaa tgtaattatg ttttaatttc atggaaatgt ttgagcataa
     4141 tttttattaa tgtactaaat tactgttttg ttaaatgcaa ttttgctttc tcgggatttt
     4201 aatatcaaaa tctatttaga aatacacaat attttgttgc aggcttgctg gagaatcgat
     4261 ctgctatcat aaaaattaca aaaaaatttt atttgcctca attattttag gattggtatt
     4321 aaggacgctt aaattatttg tcgggtcact acgcatcatt gtgattgaga agatcagcga
     4381 tacgaaatat tcgtagtact atcgataatt tatttgaaaa ttcataagaa aagcaaacgt
     4441 tacatgaatt gatgaaacaa tacaaagaca gataaagcca cgcacattta ggatattggc
     4501 cgagattact gaatattgag taagatcacg gaatttctga caggagcatg tcttcaattc
     4561 agcccaaatg gcagttgaaa tactcaaacc gccccatatg caggagcgga tcattcattg
     4621 tttgtttggt tgcctttgcc aacatgggag tccaaggttt cagggaagct ggaattccgg
     4681 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     4741 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg
     4801 atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa
     4861 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     4921 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     4981 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     5041 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     5101 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     5161 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     5221 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     5281 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     5341 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     5401 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     5461 gtcctggagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     5521 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     5581 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     5641 aatcatgcga aacgatccgg ggaattcgct agagtcgatt tggtgtatcg agattggtta
     5701 tgaaattcag atgctagtgt aatgtattgg taatttggga agatataata ggaagcaagg
     5761 ctatttatcc atttctgaaa aggcgaaatg gcgtcaccgc gagcgtcacg cgcattccgt
     5821 tcttgctgta aagcgttgtt tggtacactt ttgactagcg aggcttggcg tgtcagcgta
     5881 tctattcaaa agtcgttaat ggctgcggat caagaaaaag ttggaataga aacagaatac
     5941 ccgcgaaatt caggcccggt tgccatgtcc tacacgccga aataaacgac caaattagta
     6001 gaaaaataaa aactgactcg gatacttacg tcacgtcttg cgcactgatt tgaaaaatct
     6061 caatataaac aaagacggcc acaagaaaaa accaaaacac cgatattcat taatcttatc
     6121 tagtttctca aaaaaattca tatcttccac acgtgaaaat gccaatttct cagacctacc
     6181 tcggctctgc gaaggccccc gctggtatca aaagttttta tttcatccga catggcgcga
     6241 ccgacctcaa cgagaaggaa attgtcgtga acggtgagaa gctctggggc gtgcaaggtt
     6301 ccggaacgaa catcggtctc aatgcaaaag gggaacgcca ggctctgttg gcccctcgaa
     6361 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     6421 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     6481 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     6541 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     6601 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     6661 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     6721 tcgttcggtt cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga
     6781 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat
     6841 gacatgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     6901 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     6961 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     7021 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     7081 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     7141 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     7201 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     7261 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     7321 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     7381 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     7441 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     7501 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     7561 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     7621 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     7681 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     7741 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     7801 gccgcttcgc cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc
     7861 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     7921 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8161 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8701 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8761 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     8821 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8881 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     8941 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     9001 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     9061 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
     9121 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
     9181 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
     9241 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
     9301 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
     9361 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
     9421 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
     9481 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
     9541 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
     9601 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
     9661 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
     9721 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
     9781 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
     9841 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
     9901 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
     9961 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
    10021 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
    10081 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
    10141 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
    10201 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
    10261 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
    10321 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
    10381 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
    10441 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
    10501 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
    10561 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc
    10621 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
    10681 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
    10741 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
    10801 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
    10861 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
    10921 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
    10981 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
    11041 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
    11101 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
    11161 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
    11221 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
    11281 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
    11341 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
    11401 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
    11461 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
    11521 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
    11581 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
    11641 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    11701 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    11761 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    11821 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    11881 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    11941 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    12001 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    12061 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    12121 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    12181 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    12241 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    12301 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    12361 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    12421 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    12481 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    12541 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    12601 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    12661 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    12721 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    12781 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    12841 caaagtact
//
