LOCUS       40924_2189       12872 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary expression vector NES-YC3.6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     FRET-based genetically encoded sensors allow high-resolution live 
            cell imaging of Ca(2) dynamics
  JOURNAL   Plant J. 69 (1), 181-192 (2012)
  PUBMED    21910770
REFERENCE   2  (bases 1 to 12872)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2011) Department of Developmental Biology, Centre 
            for Organismal Studies Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 12872)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12872)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2012"; volume: "69"; issue: "1"; pages: "181-192"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2011) Department of Developmental Biology, Centre for 
            Organismal Studies Heidelberg, Im Neuenheimer Feld 230, Heidelberg 
            69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(113..137)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    302..323
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        338..368
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    376..392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     400..416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      444..1088
                     /label=AtUBQ10
                     /note="AtUBQ10"
                     /regulatory_class="promoter"
     misc_feature    1114..1174
                     /label=NES
                     /note="NES"
     CDS             1198..1881
                     /label=CFP
                     /note="cyan variant of GFP"
     CDS             2338..2415
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             2638..3156
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     terminator      3175..3816
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3829..3845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(4427..4679)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4878..5669)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5699..6079)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    complement(6451..6475)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7003..7791
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      8038..8626
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8812..8952)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9296..9490)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9559..10623)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11060..11686)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
       61 tttcacgccc ttttaaatat ccgttattct aataaacgct cttttctctt aggtttaccc
      121 gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat
      181 ccaagctcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      301 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      361 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      421 attacgaatt cgagctcggt acccgacgag tcagtaataa acggcgtcaa agtggttgca
      481 gccggcacac acgagtcgtg tttatcaact caaagcacaa atacttttcc tcaacctaaa
      541 aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt
      601 ttattattag ctattgcttc accgccttag ctttctcgtg acctagtcgt cctcgtcttt
      661 tcttcttctt cttctataaa acaataccca aagagctctt cttcttcaca attcagattt
      721 caatttctca aaatcttaaa aactttctct caattctctc taccgtgatc aaggtaaatt
      781 tctgtgttcc ttattctctc aaaatcttcg attttgtttt cgttcgatcc caatttcgta
      841 tatgttcttt ggtttagatt ctgttaatct tagatcgaag acgattttct gggtttgatc
      901 gttagatatc atcttaattc tcgattaggg tttcatagat atcatccgat ttgttcaaat
      961 aatttgagtt ttgtcgaata attactcttc gatttgtgat ttctatctag atctggtgtt
     1021 agtttctagt ttgtgcgatc gaatttgtag attaatctga gtttttctga ttaacagctc
     1081 gagtgcggga tcctctagag ggcccgggac gtcatgctgc agaacgagct tgctcttaag
     1141 ttggctggac ttgatattaa caagactgga ggagtcgact cgagtgcggc cgccaccatg
     1201 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1261 gacgtaaacg gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1321 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1381 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1441 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac catcttcttc
     1501 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1561 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1621 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     1681 atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1741 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1801 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1861 ctggagttcg tgaccgccgc ccgcatgcat gaccaactga cagaagagca gattgcagag
     1921 ttcaaagaag ccttctcatt attcgacaag gatggggacg gcaccatcac cacaaaggaa
     1981 cttggcaccg ttatgaggtc gcttggacaa aacccaacgg aagcagaatt gcaggatatg
     2041 atcaatgaag tcgatgctga tggcaatgga acgatttact ttcctgaatt tcttactatg
     2101 atggctagaa aaatgaagga cacagacagc gaagaggaaa tccgagaagc attccgtgtt
     2161 tttgacaagg atgggaacgg ctacatcagc gctgctcagt tacgtcacgt catgacaaac
     2221 ctcggggaga agttaacaga tgaagaagtt gatgaaatga taagggaagc agatatcgat
     2281 ggtgatggcc aagtaaacta tgaagagttt gtacaaatga tgacagcaaa gggggggaag
     2341 aggcgctgga agaaaaactt cattgccgtc agcgctgcca accggttcaa gaagatctcc
     2401 agctccgggg cactggagct catggacggc ggcgtgcagc tcgccgacca ctaccagcag
     2461 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     2521 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2581 accgccgccg ggatcactct cggcatggac gagctgtaca agggtggcag cggtggcatg
     2641 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2701 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2761 aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2821 gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     2881 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2941 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3001 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3061 ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     3121 atcaaggcca acttcaagat ccgccacaac atcgagtaaa ctagttggct gcagagcttt
     3181 cgttcgtatc atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt tcattgcgca
     3241 cacaccagaa tcctactgag ttcgagtatt atggcattgg gaaacatgtt tttcttgtac
     3301 catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa
     3361 atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta
     3421 atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaaatata agagatatgc
     3481 aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata
     3541 tgaggagtaa aacacttgta gttgtaccat tatgcttatt cactaggcaa caaatatatt
     3601 ttcagaccta gaaaagctgc aaatgttact gaatacaagt atgtcctctt gtgttttaga
     3661 catttatgaa ctttccttta tgtaattttc cagaatcctt gtcagattct aatcattgct
     3721 ttataattat agttatactc atggatttgt agttgagtat gaaaatattt tttaatgcat
     3781 tttatgactt gccaattgat tgacaacatg catcaatcga agcttggcac tggccgtcgt
     3841 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3901 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3961 gttgcgcagc ctgaatggcg aatgctagag cagcttgcca atcgaggccg accaaccgca
     4021 agcgttgtca gtgttgcaaa gcgctctgtg tgggcctact ttaattgctt ccagtgttaa
     4081 attggcgaaa ggcaataata tcgcaaaata ttgtgttgta aaatgtaatt atgttttaat
     4141 ttcatggaaa tgtttgagca taatttttat taatgtacta aattactgtt ttgttaaatg
     4201 caattttgct ttctcgggat tttaatatca aaatctattt agaaatacac aatattttgt
     4261 tgcaggcttg ctggagaatc gatctgctat cataaaaatt acaaaaaaat tttatttgcc
     4321 tcaattattt taggattggt attaaggacg cttaaattat ttgtcgggtc actacgcatc
     4381 attgtgattg agaagatcag cgatacgaaa tattcgtagt actatcgata atttatttga
     4441 aaattcataa gaaaagcaaa cgttacatga attgatgaaa caatacaaag acagataaag
     4501 ccacgcacat ttaggatatt ggccgagatt actgaatatt gagtaagatc acggaatttc
     4561 tgacaggagc atgtcttcaa ttcagcccaa atggcagttg aaatactcaa accgccccat
     4621 atgcaggagc ggatcattca ttgtttgttt ggttgccttt gccaacatgg gagtccaagg
     4681 tttcagggaa gctggaattc cggggtgggc gaagaactcc agcatgagat ccccgcgctg
     4741 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     4801 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     4861 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     4921 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     4981 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     5041 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     5101 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     5161 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     5221 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     5281 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     5341 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     5401 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     5461 agccacgata gccgcgctgc ctcgtcctgg agttcattca gggcaccgga caggtcggtc
     5521 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     5581 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     5641 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc cggggaattc gctagagtcg
     5701 atttggtgta tcgagattgg ttatgaaatt cagatgctag tgtaatgtat tggtaatttg
     5761 ggaagatata ataggaagca aggctattta tccatttctg aaaaggcgaa atggcgtcac
     5821 cgcgagcgtc acgcgcattc cgttcttgct gtaaagcgtt gtttggtaca cttttgacta
     5881 gcgaggcttg gcgtgtcagc gtatctattc aaaagtcgtt aatggctgcg gatcaagaaa
     5941 aagttggaat agaaacagaa tacccgcgaa attcaggccc ggttgccatg tcctacacgc
     6001 cgaaataaac gaccaaatta gtagaaaaat aaaaactgac tcggatactt acgtcacgtc
     6061 ttgcgcactg atttgaaaaa tctcaatata aacaaagacg gccacaagaa aaaaccaaaa
     6121 caccgatatt cattaatctt atctagtttc tcaaaaaaat tcatatcttc cacacgtgaa
     6181 aatgccaatt tctcagacct acctcggctc tgcgaaggcc cccgctggta tcaaaagttt
     6241 ttatttcatc cgacatggcg cgaccgacct caacgagaag gaaattgtcg tgaacggtga
     6301 gaagctctgg ggcgtgcaag gttccggaac gaacatcggt ctcaatgcaa aaggggaacg
     6361 ccaggctctg ttggcccctc gaaattcggc gttaattcag tacattaaaa acgtccgcaa
     6421 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     6481 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     6541 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     6601 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     6661 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
     6721 cacgaaccca gtggacataa gcctcgttcg gttcgtaagc tgtaatgcaa gtagcgtaac
     6781 tgccgtcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     6841 cggttttcat ggcttcttgt tatgacatgt ttttttgggg tacagtctat gcctcgggca
     6901 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     6961 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
     7021 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     7081 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     7141 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     7201 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     7261 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     7321 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     7381 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     7441 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     7501 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     7561 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     7621 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     7681 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     7741 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     7801 gctagaaatt cgttcaagcc gacgccgctt cgccggcgtt aactcaagcg attagatgca
     7861 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     7921 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta
     7981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8161 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     8221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8701 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8761 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     8821 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     8881 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     8941 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9001 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     9061 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     9121 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     9181 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     9241 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     9301 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     9361 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     9421 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     9481 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     9541 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     9601 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     9661 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     9721 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     9781 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     9841 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     9901 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     9961 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
    10021 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
    10081 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
    10141 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
    10201 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
    10261 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
    10321 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
    10381 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
    10441 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
    10501 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
    10561 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
    10621 accgtcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
    10681 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
    10741 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
    10801 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
    10861 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    10921 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    10981 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    11041 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    11101 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    11161 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    11221 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    11281 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    11341 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    11401 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    11461 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    11521 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    11581 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    11641 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    11701 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    11761 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    11821 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    11881 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    11941 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    12001 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    12061 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    12121 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    12181 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    12241 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    12301 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    12361 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    12421 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    12481 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    12541 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    12601 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    12661 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    12721 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    12781 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    12841 ctatagcagc ggaggggttg gatcaaagta ct
//
