LOCUS       Exported                4776 bp DNA     circular SYN 08-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        688..1172
                     /label=Adenovirus TPL and MLP
                     /label=Adenovirus Tripartite Leader and Major Late
                     Promoter
                     /note="Regulatory DNA sequence from adenovirus containing
                     both the tripartite leader and major late promoter 
                     elements, functioning to initiate and enhance transcription
                     of late viral genes.; id: 3685243"
     sig_peptide     1236..1289
                     /product="human interleukin 10 signal peptide"
     CDS             1302..1625
                     /codon_start=1
                     /product="constant region of mouse immunoglobulin kappa 
                     chain"
                     /label=mouse IgKappa
                     /translation="RADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDG
                     SERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR
                     NEC"
     polyA_site      1652..1707
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    2020..2747
                     /label=OriP
                     /note="Epstein-Barr virus (EBV) origin of replication"
     promoter        2876..2980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2981..3841
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4012..4600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagagcg
     1201 gccgcaaact acaagacaga cttgcaaaag aaggcatgca cagctcagca ctgctctgtt
     1261 gcctggtcct cctgactggg gtgagggccg atatcggtac cagagctgat gccgctccta
     1321 ccgtgtccat ctttccacct agcagcgagc agctgacaag cggcggagct agcgtcgtgt
     1381 gcttcctgaa caacttctac cccaaggaca tcaacgtgaa gtggaagatc gacggcagcg
     1441 agagacagaa cggcgtgctg aatagctgga ccgaccagga cagcaaggac tccacctaca
     1501 gcatgtccag cacactgacc ctgaccaagg acgagtacga gcggcacaac agctacacat
     1561 gcgaggccac acacaagacc agcacaagcc ccatcgtgaa gtccttcaac cggaacgagt
     1621 gctgaggatc ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata
     1681 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg
     1741 gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga cgaactaaac
     1801 ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt
     1861 tggtacaact tgccaactga accctaaacg ggtagcatat gcttcccggg tagtagtata
     1921 tactatccag actaacccta attcaatagc atatgttacc caacgggaag catatgctat
     1981 cgaattaggg ttagtaaaag ggtcctaagg aacagcgatg taggtgggcg ggccaagata
     2041 ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc
     2101 acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc
     2161 atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     2221 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     2281 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     2341 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     2401 gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg
     2461 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     2521 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     2581 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     2641 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     2701 tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata agctgtcaaa
     2761 catgagaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2821 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2881 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2941 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3001 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3061 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3121 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3181 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     3241 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3301 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3361 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3421 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3481 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     3541 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3601 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3661 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3721 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3781 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3841 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3901 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3961 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4021 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4081 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4141 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4201 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4261 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4321 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4381 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4441 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4501 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4561 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4621 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4681 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4741 aacgaccgag cgcagcgagt cagtgagcga ggaagc
//
