LOCUS       Exported                5817 bp DNA     circular SYN 08-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Dec 8, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        688..1172
                     /label=Adenovirus TPL and MLP
                     /label=Adenovirus Tripartite Leader and Major Late
                     Promoter
                     /note="Regulatory DNA sequence from adenovirus containing
                     both the tripartite leader and major late promoter 
                     elements, functioning to initiate and enhance transcription
                     of late viral genes.; id: 3685243"
     sig_peptide     1236..1289
                     /product="human interleukin 10 signal peptide"
     CDS             1302..2666
                     /codon_start=1
                     /product="immunoglobulin mu heavy chain"
                     /label=muIgM
                     /translation="SQSFPNVFPLVSCESPLSDKNLVAMGCLARDFLPSTISFTWNYQN
                     NTEVIQGIRTFPTLRTGGKYLATSQVLLSPKSILEGSDEYLVCKIHYGGKNRDLHVPIP
                     AVAEMNPNVNVFVPPRDGFSGPAPRKSKLICEATNFTPKPITVSWLKDGKLVESGFTTD
                     PVTIENKGSTPQTYKVISTLTISEIDWLNLNVYTCRVDHRGLTFLKNVSSTCAASPSTD
                     ILTFTIPPSFADIFLSKSANLTCLVSNLATYETLNISWASQSGEPLETKIKIMESHPNG
                     TFSAKGVASVCVEDWNNRKEFVCTVTHRDLPSPQKKFISKPNEVHKHPPAVYLLPPARE
                     QLNLRESATVTCLVKGFSPADISVQWLQRGQLLPQEKYVTSAPMPEPGAPGFYFTHSIL
                     TVTEEEWNSGETYTCVVGHEALPHLVTERTVDKSTGKPTLYNVSLIMSDTGGTCY"
     polyA_site      2693..2748
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    3061..3788
                     /label=OriP
                     /note="Epstein-Barr virus (EBV) origin of replication"
     promoter        3917..4021
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4022..4882
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5053..5641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
       61 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      121 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      181 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      241 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      301 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      361 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      421 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      481 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      541 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      601 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcctca ctctcttccg
      661 catcgctgtc tgcgagggcc agctgttggg ctcgcggttg aggacaaact cttcgcggtc
      721 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg
      781 acctgagcga gtccgcatcg accggatcgg aaaacctctc gagaaaggcg tctaaccagt
      841 cacagtcgca aggtaggctg agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt
      901 ttctggcgga ggtgctgctg atgatgtaat taaagtaggc ggtcttgaga cggcggatgg
      961 tcgaggtgag gtgtggcagg cttgagatcc agctgttggg gtgagtactc cctctcaaaa
     1021 gcgggcatta cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc
     1081 acctggcccg atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1141 acaggtgtcc actcccaggt ccaagtttaa acggatctct agcgaattcc ctctagagcg
     1201 gccgcaaact acaagacaga cttgcaaaag aaggcatgca cagctcagca ctgctctgtt
     1261 gcctggtcct cctgactggg gtgagggccg atatcggtac cagccagagc ttccccaacg
     1321 tgttccctct ggtgtcttgc gagagccctc tgagcgacaa gaacctggtg gccatgggct
     1381 gcctggccag agattttctg cctagcacca tcagcttcac ctggaactac cagaacaaca
     1441 ccgaagtgat ccagggcatc agaacattcc ccacactgag aaccggcggc aagtacctgg
     1501 ctacatctca ggtgctgctg agccccaaga gcatcctgga aggctccgat gagtacctcg
     1561 tgtgcaagat ccactatggc ggcaagaacc gggatctgca cgtgccaatt ccagccgtgg
     1621 ccgagatgaa ccccaatgtg aacgtgttcg tgcctcctcg cgacggcttt tctggacccg
     1681 ctcctagaaa gagcaagctg atctgcgagg ccaccaactt cacccctaag cctatcaccg
     1741 tgtcctggct gaaggacggc aagctggtgg aaagcggctt caccaccgat cctgtgacca
     1801 tcgagaacaa gggcagcacc cctcagacct acaaagtgat cagcaccctg accatcagcg
     1861 agatcgactg gctgaacctg aacgtgtaca cctgtagagt ggaccaccgg ggcctgacct
     1921 ttctgaagaa cgtgtccagc acctgtgccg cctctccaag caccgacatc ctgaccttca
     1981 caatccctcc aagcttcgcc gacatcttcc tgagcaagag cgccaacctg acctgcctgg
     2041 tgtctaacct ggccacctac gagacactga acatcagctg ggccagccag tctggcgagc
     2101 ccctggaaac aaagatcaag atcatggaat ctcaccccaa cggcaccttc agcgctaaag
     2161 gcgtggcctc tgtgtgcgtg gaagattgga acaaccggaa agaatttgtg tgcaccgtga
     2221 cacaccggga cctgccttct ccacagaaga agttcatcag caagcccaac gaggtgcaca
     2281 agcacccacc agccgtttac ttgctgcctc ctgccagaga gcagctgaat ctgagagaaa
     2341 gcgccaccgt gacttgcctc gtgaagggat tttccccagc cgacatcagc gtgcagtggc
     2401 tgcaaagagg acagctgctg cctcaagaga aatacgtgac aagcgcccct atgcctgagc
     2461 ctggtgctcc tggcttctac ttcacccaca gcatcctgac agtgaccgag gaagagtgga
     2521 actccggcga gacatacacc tgtgttgtgg gccatgaagc cctgcctcac ctggtcaccg
     2581 agagaaccgt ggataagagc accggcaagc ccacactgta caacgtgtcc ctgatcatga
     2641 gcgacaccgg cggaacctgt tactgaggat cccccgacct cgacctctgg ctaataaagg
     2701 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     2761 tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc
     2821 cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg
     2881 catgtaatcc cttcagttgg ttggtacaac ttgccaactg aaccctaaac gggtagcata
     2941 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
     3001 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
     3061 gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac
     3121 acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga
     3181 gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat
     3241 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     3301 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     3361 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     3421 gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat
     3481 gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta
     3541 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta
     3601 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     3661 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     3721 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     3781 tcctcatgat aagctgtcaa acatgagaat taattcttga agacgaaagg gcctcgtgat
     3841 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     3901 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3961 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4021 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4081 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4141 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4201 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4261 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4321 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4381 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4441 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4501 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4561 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4621 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4681 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4741 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4801 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4861 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4921 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4981 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5041 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5101 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5161 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5221 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5281 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5341 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5401 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5461 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5521 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5581 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5641 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5701 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5761 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagc
//
