LOCUS       40924_1989       13902 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Tri-shuttle cloning vector MiniTn-Puro-JCVI-1.7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Benders GA, Noskov VN, Denisova EA, Lartigue C, Gibson DG, 
            Assad-Garcia N, Chuang RY, Carrera W, Moodie M, Algire MA, Phan Q, 
            Alperovich N, Vashee S, Merryman C, Venter JC, Smith HO, Glass JI, 
            Hutchison CA III.
  TITLE     Cloning whole bacterial genomes in yeast
  JOURNAL   Nucleic Acids Res. 38 (8), 2558-2569 (2010)
  PUBMED    20211840
REFERENCE   2  (bases 1 to 13902)
  AUTHORS   Gibson DG, Benders GA, Lartigue C, Glass JI.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2010) Synthetic Biology, J. Craig Venter 
            Institute, 10355 Science Center Dr., San Diego, CA 92121, USA
REFERENCE   3  (bases 1 to 13902)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13902)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010"; volume: "38"; issue: "8"; pages: 
            "2558-2569"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2010) Synthetic Biology, J. Craig Venter Institute, 10355 
            Science Center Dr., San Diego, CA 92121, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(1..26)
                     /mobile_element_type="insertion sequence:IS256"
                     /label=insertion sequence:IS256
                     /note="inner inverted repeat"
     promoter        complement(45..63)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(84..100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(108..124)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(132..162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(177..198)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(486..1074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1248..2105)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2106..2210)
                     /label=AmpR promoter
     rep_origin      complement(2236..2691)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2833..2849
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2856..2874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2895..4067)
                     /codon_start=1
                     /product="IS256 transposase"
                     /label=IS256 transposase
                     /protein_id="ADE19332.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTEY
                     IQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKALM
                     ASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPY
                     LMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKER
                     GLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGI
                     FKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLI
                     ERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     mobile_element  4173..4197
                     /mobile_element_type="insertion sequence:IS256"
                     /label=insertion sequence:IS256
                     /note="outer inverted repeat"
     protein_bind    4347..4380
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      4402..4431
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      4462..5091
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      5165..5384
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             5475..6227
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    6233..6483
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6809..7981
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             7984..8952
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    9028..9501
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(9775..10587)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(10588..10690)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    complement(10712..11110)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     terminator      complement(11159..11190)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     protein_bind    11209..11242
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(11352..12008)
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     promoter        complement(12009..12196)
                     /label=HIS3 promoter
     misc_feature    complement(12197..12700)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        12703..12721
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      12746..13068
                     /label=spiralin promoter
                     /note="spiralin promoter"
                     /regulatory_class="promoter"
     CDS             13070..13666
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
ORIGIN
        1 ttttacacag gagtctggac ttgactgagc tccagctttt gttcccttta gtgagggtta
       61 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      121 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      181 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      241 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      301 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      361 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      421 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      481 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      541 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      601 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      661 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      721 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      781 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      841 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      901 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      961 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1021 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1081 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1141 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1201 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1261 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1321 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1381 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1441 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1501 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1561 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1621 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1681 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1741 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1801 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1861 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1921 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1981 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2041 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2101 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2161 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2221 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2281 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2341 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2401 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2461 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2521 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2581 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2641 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     2701 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2761 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2821 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
     2881 tgggtacccc atttctactt atcaaaattg atgtattttc ttgaagaata aatccattca
     2941 tcatgtaggt ccataagaac ggctccaatt aagcgattgg ctgatgtttg attggggaag
     3001 atgcgaataa tcttttctct tctgcgtact tcttgattca gtcgttcaat tagattggta
     3061 ctctttagtc gattgtggga atttccttgt acggtatatt gaaaggcgtc ttcgaatcca
     3121 tcatccaatg atgcgcaagc ttttgaatat tttggttgat cgatataatc atgaatcaat
     3181 cgatttttag cctcacgcgc taagttaata tctgtgaact taaaaattcc tttaacagct
     3241 tctctgaaag attttgaatt ttttttagga atggtggtaa agatatttct taggaagtga
     3301 acttggcatc tttgccaact tacgttggtg aaggattttc taatggcaga gactaatcct
     3361 ttgtgcgcat cagaaataac gagttccgta ccttgtaaac cgcgttcttt taggtattca
     3421 aaaaatgttg tccaggtctc ttcgctttcg ccactttgaa tcatgaagcc gataatttca
     3481 cggtcgccat ctttggttat tccaatcgct atatgacagc tttttgagag tactcgattt
     3541 tcttctcgta cttttatata gagtacatcg gtcattaagt aaggataatt tttttctgat
     3601 aataaacgat tctgccactc gttaaccata ggttctagct gttctgttaa gctagaaacg
     3661 aaggacttag agacggattt accacaaagt tcttccacaa tttttgatac tttacgagtt
     3721 gaaacgcctg atacatacat ttccaacatt gaagccatga gggctttttc gtttcgttga
     3781 taacgttcaa acactgtggg tgaaaaatgg ccatcacgtg ttctgggtac ttttaattct
     3841 agcgtgccta cacgtgtcgt aaagctgcgc tcataatagc catttcgttg actttgtcgg
     3901 ttttctgttc gttcatattc ttttgcttga atatattctg ttcgttgatt ttccattagt
     3961 tgattaaata ccgttgttaa aatattttta gaaacgtcat cctttacaga atattcaata
     4021 atgctttgaa tctcttcgct tttcagtgta aaatgtactt gggtcatgta aaagtcctcc
     4081 tgggtatgtt tttgtcgtta aaaacattgt accgtaaaag gactgttata tggccttttt
     4141 acttttacac gtcgacggta tcgataagct tgataaagtc cgtataattg tgtaaaagaa
     4201 ttcgagctcg gtacccgggg atccgatcct ctagagtcga cctgcaggcg gccgctttaa
     4261 ttattgctag ttatataggg gttagaactt catttttcct tgtttatcga tgcaaccttc
     4321 tttctctctt ctggcgcgcc tagtccataa cttcgtataa tgtatgctat acgaagttat
     4381 ttacgtagca atcaactcac tggctcacct tcacgggtgg gcctttcttc ggcacgggca
     4441 aattgctgaa tattcctttc cgacgctcac cgggctggtt gccctcgccg ctgggctggc
     4501 ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg
     4561 cggccggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag
     4621 gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc
     4681 aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct
     4741 gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt
     4801 attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg
     4861 ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca
     4921 gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt
     4981 aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg
     5041 cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg gtcgagtgag
     5101 cgaggaagca ccagggaaca gcacttatat attctgctta cacacgatgc ctgaaaaaac
     5161 ttcccttggg gttatccact tatccacggg gatattttta taattatttt ttttatagtt
     5221 tttagatctt cttttttaga gcgccttgta ggcctttatc catgctggtt ctagagaagg
     5281 tgttgtgaca aattgccctt tcagtgtgac aaatcaccct caaatgacag tcctgtctgt
     5341 gacaaattgc ccttaaccct gtgacaaatt gccctcagaa gaagctgttt tttcacaaag
     5401 ttatccctgc ttattgactc ttttttattt agtgtgacaa tctaaaaact tgtcacactt
     5461 cacatggatc tgtcatggcg gaaacagcgg ttatcaatca caagaaacgt aaaaatagcc
     5521 cgcgaatcgt ccagtcaaac gacctcactg aggcggcata tagtctctcc cgggatcaaa
     5581 aacgtatgct gtatctgttc gttgaccaga tcagaaaatc tgatggcacc ctacaggaac
     5641 atgacggtat ctgcgagatc catgttgcta aatatgctga aatattcgga ttgacctctg
     5701 cggaagccag taaggatata cggcaggcat tgaagagttt cgcggggaag gaagtggttt
     5761 tttatcgccc tgaagaggat gccggcgatg aaaaaggcta tgaatctttt ccttggttta
     5821 tcaaacgtgc gcacagtcca tccagagggc tttacagtgt acatatcaac ccatatctca
     5881 ttcccttctt tatcgggtta cagaaccggt ttacgcagtt tcggcttagt gaaacaaaag
     5941 aaatcaccaa tccgtatgcc atgcgtttat acgaatccct gtgtcagtat cgtaagccgg
     6001 atggctcagg catcgtctct ctgaaaatcg actggatcat agagcgttac cagctgcctc
     6061 aaagttacca gcgtatgcct gacttccgcc gccgcttcct gcaggtctgt gttaatgaga
     6121 tcaacagcag aactccaatg cgcctctcat acattgagaa aaagaaaggc cgccagacga
     6181 ctcatatcgt attttccttc cgcgatatca cttccatgac gacaggatag tctgagggtt
     6241 atctgtcaca gatttgaggg tggttcgtca catttgttct gacctactga gggtaatttg
     6301 tcacagtttt gctgtttcct tcagcctgca tggattttct catacttttt gaactgtaat
     6361 ttttaaggaa gccaaatttg agggcagttt gtcacagttg atttccttct ctttcccttc
     6421 gtcatgtgac ttgatatcgg gggttagttc gtcatcattg atgagggttg attatcacag
     6481 tttattactc tgaattggct atccgcgtgt gtacctctac ctggagtttt tcccacggtg
     6541 gatatttctt cttgcgctga gcgtaagagc tatctgacag aacagttctt ctttgcttcc
     6601 tcgccagttc gctcgctatg ctcggttaca cggctgcggc gagcgctagt gataataagt
     6661 gactgaggta tgtgctcttc ttatctcctt ttgtagtgtt gctcttattt taaacaactt
     6721 tgcggttttt tgatgacttt gcgattttgt tgttgctttg cagtaaattg caagatttaa
     6781 taaaaaaacg caaagcaatg attaaaggat gttcagaatg aaactcatgg aaacacttaa
     6841 ccagtgcata aacgctggtc atgaaatgac gaaggctatc gccattgcac agtttaatga
     6901 tgacagcccg gaagcgagga aaataacccg gcgctggaga ataggtgaag cagcggattt
     6961 agttggggtt tcttctcagg ctatcagaga tgccgagaaa gcagggcgac taccgcaccc
     7021 ggatatggaa attcgaggac gggttgagca acgtgttggt tatacaattg aacaaattaa
     7081 tcatatgcgt gatgtgtttg gtacgcgatt gcgacgtgct gaagacgtat ttccaccggt
     7141 gatcggggtt gctgcccata aaggtggcgt ttacaaaacc tcagtttctg ttcatcttgc
     7201 tcaggatctg gctctgaagg ggctacgtgt tttgctcgtg gaaggtaacg acccccaggg
     7261 aacagcctca atgtatcacg gatgggtacc agatcttcat attcatgcag aagacactct
     7321 cctgcctttc tatcttgggg aaaaggacga tgtcacttat gcaataaagc ccacttgctg
     7381 gccggggctt gacattattc cttcctgtct ggctctgcac cgtattgaaa ctgagttaat
     7441 gggcaaattt gatgaaggta aactgcccac cgatccacac ctgatgctcc gactggccat
     7501 tgaaactgtt gctcatgact atgatgtcat agttattgac agcgcgccta acctgggtat
     7561 cggcacgatt aatgtcgtat gtgctgctga tgtgctgatt gttcccacgc ctgctgagtt
     7621 gtttgactac acctccgcac tgcagttttt cgatatgctt cgtgatctgc tcaagaacgt
     7681 tgatcttaaa gggttcgagc ctgatgtacg tattttgctt accaaataca gcaatagtaa
     7741 tggctctcag tccccgtgga tggaggagca aattcgggat gcctggggaa gcatggttct
     7801 aaaaaatgtt gtacgtgaaa cggatgaagt tggtaaaggt cagatccgga tgagaactgt
     7861 ttttgaacag gccattgatc aacgctcttc aactggtgcc tggagaaatg ctctttctat
     7921 ttgggaacct gtctgcaatg aaattttcga tcgtctgatt aaaccacgct gggagattag
     7981 ataatgaagc gtgcgcctgt tattccaaaa catacgctca atactcaacc ggttgaagat
     8041 acttcgttat cgacaccagc tgccccgatg gtggattcgt taattgcgcg cgtaggagta
     8101 atggctcgcg gtaatgccat tactttgcct gtatgtggtc gggatgtgaa gtttactctt
     8161 gaagtgctcc ggggtgatag tgttgagaag acctctcggg tatggtcagg taatgaacgt
     8221 gaccaggagc tgcttactga ggacgcactg gatgatctca tcccttcttt tctactgact
     8281 ggtcaacaga caccggcgtt cggtcgaaga gtatctggtg tcatagaaat tgccgatggg
     8341 agtcgccgtc gtaaagctgc tgcacttacc gaaagtgatt atcgtgttct ggttggcgag
     8401 ctggatgatg agcagatggc tgcattatcc agattgggta acgattatcg cccaacaagt
     8461 gcttatgaac gtggtcagcg ttatgcaagc cgattgcaga atgaatttgc tggaaatatt
     8521 tctgcgctgg ctgatgcgga aaatatttca cgtaagatta ttacccgctg tatcaacacc
     8581 gccaaattgc ctaaatcagt tgttgctctt ttttctcacc ccggtgaact atctgcccgg
     8641 tcaggtgatg cacttcaaaa agcctttaca gataaagagg aattacttaa gcagcaggca
     8701 tctaaccttc atgagcagaa aaaagctggg gtgatatttg aagctgaaga agttatcact
     8761 cttttaactt ctgtgcttaa aacgtcatct gcatcaagaa ctagtttaag ctcacgacat
     8821 cagtttgctc ctggagcgac agtattgtat aagggcgata aaatggtgct taacctggac
     8881 aggtctcgtg ttccaactga gtgtatagag aaaattgagg ccattcttaa ggaacttgaa
     8941 aagccagcac cctgatgcga ccacgtttta gtctacgttt atctgtcttt acttaatgtc
     9001 ctttgttaca ggccagaaag cataactggc ctgaatattc tctctgggcc cactgttcca
     9061 cttgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg
     9121 attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
     9181 acggtcccac tcgtatcgtc ggtctgataa tcagactggg accacggtcc cactcgtatc
     9241 gtcggtctga ttattagtct gggaccatgg tcccactcgt atcgtcggtc tgattattag
     9301 tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggaa ccacggtccc
     9361 actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct
     9421 gattattagt ctgggaccac gatcccactc gtgttgtcgg tctgattatc ggtctgggac
     9481 cacggtccca cttgtattgt cgatcagact atcagcgtga gactacgatt ccatcaatgc
     9541 ctgtcaaggg caagtattga catgtcgtcg taacctgtag aacggagtaa cctcggtgtg
     9601 cggttgtatg cctgctgtgg attgctgctg tgtcctgctt atccacaaca ttttgcgcac
     9661 ggttatgtgg acaaaatacc tggttaccca ggccgtgccg gcacgttcac cgggctgcat
     9721 ccgatgcaag tgtgaaaggc ccacccgaag gtgagccagg tgattacatt tttagaaaaa
     9781 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     9841 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     9901 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     9961 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
    10021 tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
    10081 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
    10141 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
    10201 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
    10261 tacctggaat gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt
    10321 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
    10381 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
    10441 cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg
    10501 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgagca
    10561 agacgtttcc cgttgaatat ggctcatttt agcttcctta gctcctgaaa atctcgataa
    10621 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagtcgg aacctcttac
    10681 gtgccgatca gggcggtctc actcttcagg cgacatgagg ttgccccgta ttcagtgtcg
    10741 ctgatttgta ttgtctgaag ttgcttttac gttaagttga tgcagatcaa ttaatacgat
    10801 acctgcgtca taattgatta tttgacgtgg tttgatggcc tccacgcacg ttgtgatatg
    10861 tagatgataa tcattatcac tttacgggtc ctttccggtg atccgacagg ttacggggcg
    10921 gcgacctcgc gggttttcgc tatttatgaa aattttccgg tttaaggcgt ttccgttctt
    10981 cttcgtcata acttaatgtt tttatttaaa ataccctctg aaaagaaagg aaacgacagg
    11041 tgctgaaagc gagctttttg gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg
    11101 aacaatggaa gtcctcttca ggcggtctcg gcccaatgat aaccccaagg gaagttttag
    11161 tcaaaagcct ccggtcggag gcttttgact ttctgctaat gtttaaacat aacttcgtat
    11221 aatgtatgct atacgaagtt atgtcaagtg gccgccctgg cagttacaac taggaacctc
    11281 tagaggaatt cgcacgtgcg tcggatccat cgttaacgat acggtcaagt ccagactcct
    11341 gtgtaaaact acataagaac acctttggtg gagggaacat cgttggtacc attgggcgag
    11401 gtggcttctc ttatggcaac cgcaagagcc ttgaacgcac tctcactacg gtgatgatca
    11461 ttcttgcctc gcagacaatc aacgtggagg gtaattctgc tagcctctgc aaagctttca
    11521 agaaaatgcg ggatcatctc gcaagagaga tctcctactt tctccctttg caaaccaagt
    11581 tcgacaactg cgtacggcct gttcgaaaga tctaccaccg ctctggaaag tgcctcatcc
    11641 aaaggcgcaa atcctgatcc aaaccttttt actccacgcg ccagtagggc ctctttaaaa
    11701 gcttgaccga gagcaatccc gcagtcttca gtggtgtgat ggtcgtctat gtgtaagtca
    11761 ccaatgcact caacgattag cgaccagccg gaatgcttgg ccagagcatg tatcatatgg
    11821 tccagaaacc ctatacctgt gtggacgtta atcacttgcg attgtgtggc ctgttctgct
    11881 actgcttctg cctctttttc tgggaagatc gagtgctcta tcgctagggg accacccttt
    11941 aaagagatcg caatctgaat cttggtttca tttgtaatac gctttactag ggctttctgc
    12001 tctgtcatct ttgccttcgt ttatcttgcc tgctcatttt ttagtatatt cttcgaagaa
    12061 atcacattac tttatataat gtataattca ttatgtgata atgccaatcg ctaagaaaaa
    12121 aaaagagtca tccgctaggt ggaaaaaaaa aaatgaaaat cattaccgag gcataaaaaa
    12181 atatagagtg tactaggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga
    12241 tggaataatt tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt
    12301 agaaagtaaa taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt
    12361 ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga
    12421 ttaaatagat atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt
    12481 gtggtcttct acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagag
    12541 caagataaaa ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac
    12601 aaaaactatt ttttctttaa tttctttttt tactttctat ttttaattta tatatttata
    12661 ttaaaaaatt taaattataa ttatttttat agcacgtgat ggaattaacc ctcactaaag
    12721 ggaacaaaag cttgcatgcc tgcagaatta aaagttagtg aacaagaaaa cagtgaagca
    12781 ccagtttctg aaccaaaaga agacgaaaaa acaaaaaaag attaagcaat ttatttggaa
    12841 aatctttttt tgttttttta agaaatattt attgtttttt ttaaaaaatt atgtacaatt
    12901 gctactataa gggaaagaaa aaaagaaaga tataaattgt ataaagtagg gttagaagca
    12961 attaataatt attattaatg ttatttttct cttatatatt caatgtaatt ttaattacat
    13021 ttgcttttaa taaaaacact acttaataga gaaaggaaat ataagatcta tgactgaata
    13081 taaacctact gttagattag ctactagaga tgatgttcct agagctgtta gaactttagc
    13141 tgctgctttt gctgattatc ctgctactag acatactgtt gatcctgata gacatattga
    13201 aagagttact gaattacaag aattattttt aactagagtt ggtttagata ttggtaaagt
    13261 ttgggttgct gatgatggtg ctgctgttgc tgtttggact actcctgaaa gtgttgaagc
    13321 tggtgctgtt tttgctgaaa ttggtcctag aatggctgaa ttaagtggta gtagattagc
    13381 tgctcaacaa caaatggaag gtttattagc tcctcataga cctaaagaac ctgcttggtt
    13441 tttagctact gttggtgtta gtcctgatca tcaaggtaaa ggtttaggta gtgctgttgt
    13501 tttacctggt gttgaagctg ctgaaagagc tggtgttcct gcttttttag aaactagtgc
    13561 tcctagaaat ttaccttttt atgaaagatt aggttttact gttactgctg atgttgaagt
    13621 tcctgaaggt cctagaactt ggtgtatgac tagaaaacct ggtgcttaaa gatctaaatt
    13681 aaagttggtt cattcaaagt tacaattcgt ttaaaaagtg aataattaat tattaattaa
    13741 tttattaaaa aacaaccaaa aggttgtttt ttatttttta aaggatctga actgcaggtc
    13801 gactcgatgc aaaccaaaaa taagaaaaaa actttcactt attattgata tcctttcagg
    13861 aatgcggccg cgaactgcag gcatgcaagc ggatccacta gt
//
