LOCUS       .                       7658 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..517
                     /label="5' LTR"
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     CDS             1020..1427
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1437..1807
                     /label="pol region"
                     /note="Moloney murine leukemia virus (MMLV) pol region
                     containing the splice acceptor site"
     CDS             3262..3969
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     LTR             4043..4557
                     /label="3' LTR"
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(4729..4745)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4753..4783)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4798..4819)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5107..5695)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5869..6726)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6727..6831)
                     /label="AmpR promoter"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc
      781 gacctgagtc ctaaaatccc gatcgtttag gactctttgg tgcacccccc ttagaggagg
      841 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
      901 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt
      961 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg ggctagcctg
     1021 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
     1081 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
     1141 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
     1201 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
     1261 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
     1321 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
     1381 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat
     1441 atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa
     1501 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg
     1561 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc
     1621 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac
     1681 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg
     1741 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat
     1801 cctctagccc tcgagaagct tgccaccatg ggtgtcccta cccagctcct gggactgctc
     1861 ctgctgtgga tcaccgacgc catctgcgac atccagatga cccagagccc tgccagcctg
     1921 tctaccagcc tgggcgagac agtgaccatc cagtgtcagg ccagcgagga catctactct
     1981 ggcctggctt ggtatcagca gaagcccggc aagagccctc agctgctgat ctacggcgcc
     2041 agcgacctgc aggacggcgt gcctagcaga ttcagcggca gcggctccgg aacccagtac
     2101 agcctgaaga tcaccagcat gcagaccgag gacgagggcg tgtacttctg ccagcaaggc
     2161 ctgacctacc ctagaacctt cggaggaggc accaagctgg aactgaaggg cggaggcgga
     2221 agtggaggcg gaggatctgg cggcggaggc tctgaagtgc agctgcagca gtctggcgct
     2281 gaactggtcc ggcctggcac tagcgtgaag ctgtcctgca aggtgtccgg cgacaccatc
     2341 accttctact acatgcactt cgtgaagcag aggccaggac agggcctgga atggatcggc
     2401 agaatcgacc ctgaggacga gagcaccaag tacagcgaga agttcaagaa caaggccacc
     2461 ctgaccgccg acaccagcag caacaccgcc tacctgaagc tgtctagcct gacctccgag
     2521 gacaccgcca cctacttttg catctacggc ggctactact tcgactactg gggccagggc
     2581 gtgatggtca ccgtgtccag catcgagttc atgtaccccc ctccctacct ggacaacgag
     2641 agaagcaacg gcaccatcat ccacatcaaa gaaaagcacc tgtgccacac ccagagcagc
     2701 cccaagctgt tctgggccct ggtggtggtg gccggcgtgc tgttctgtta cggcctgctg
     2761 gtcacagtgg ccctgtgcgt gatctggacc aacagcagaa gaaacagagg cggccagagc
     2821 gactacatga acatgacccc cagaaggcca ggcctgacca gaaagcccta ccagccctac
     2881 gcccctgcca gagacttcgc cgcctacaga cccagagcca agttcagcag atccgccgag
     2941 acagccgcca acctgcagga tcccaaccag ctgtacaacg agctgaacct gggcagacgg
     3001 gaggaatacg acgtgctgga aaagaagaga gccagggacc ccgagatggg cggcaagcag
     3061 cagagaagaa gaaaccctca ggaaggcgtc tacaacgccc tgcagaaaga caagatggcc
     3121 gaggcctaca gcgagatcgg caccaagggc gagagaagaa ggggcaaggg ccacgatggc
     3181 ctgtaccagg gcctgtccac cgccaccaag gacacctacg acgccctgca catgcagacc
     3241 ctggccccca gatcaggatc tatggtgagc aagggcgagg aggacaacat ggccatcatc
     3301 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     3361 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     3421 accaagggcg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     3481 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     3541 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     3601 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     3661 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     3721 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     3781 gacggcggcc actacgacgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
     3841 ctgcccggcg cctacaacgt caacatcaag ctggacatca cctcccacaa cgaggactac
     3901 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgg catggacgag
     3961 ctgtacaagt gagtcgacgg taccgcgggc ccgggatccg ataaaataaa agattttatt
     4021 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     4081 agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca
     4141 aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt
     4201 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt
     4261 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     4321 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     4381 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg
     4441 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     4501 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg
     4561 taacagtttc ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat
     4621 cctgactcaa ttagccactg ttttgaatcc acatactcca atactcctga aatccatcga
     4681 tggagttcat tatggacagc gcagaaagag ctggggagaa ttgtgaaatt gttatccgct
     4741 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4801 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4861 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5521 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6721 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6841 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6901 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     6961 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7021 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     7081 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     7141 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     7201 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     7261 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca
     7321 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     7381 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     7441 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     7501 atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag
     7561 gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata
     7621 aaataaaaga ttttatttag tctccagaaa aagggggg
//
