LOCUS       62056_1625        7077 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    600..665
                     /label=MCS
                     /note="multiple cloning site"
     intron          679..1154
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1212..1628
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     CDS             2811..3740
                     /codon_start=1
                     /label=emiRFP670
                     /note="emiRFP670 is a basic (constitutively fluorescent)
                     near ir fluorescent protein published in 2020, derived from
                     Rhodopseudomonas palustris. It has low acid sensitivity. It
                     requires the cofactor biliverdin for fluorescence."
                     /translation="MAEGSVARQPDLLTCEHEEIHLAGSIQPHGALLVVSEHDHRVIQA
                     SANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLM
                     HRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVM
                     VYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVP
                     LEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHY
                     LPRFIRFELRAICKRLAERIATRITALES"
     polyA_signal    3865..3986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3993..4448)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4475..4579
                     /label=AmpR promoter
     promoter        4581..4938
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4973..5764
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5999..6046
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6375..6963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc cccttctttt
      721 ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat attgaccaaa
      781 tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat atactttttt
      841 gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata atgatacaat
      901 gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg gttaaggcaa
      961 tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg taagaggttt
     1021 catattgcta atagcagcta caatccagct accattctgc ttttatttta tggttgggat
     1081 aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta
     1141 tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat cactttggca
     1201 aagaattcat gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1261 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1321 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1381 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1441 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1501 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1561 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1621 taggcgccaa acctaaacct caagttcttt ctgacagtgg ggggccgctc atcgacctac
     1681 ttacagaaga ccccccgcct tatagggacc caagaccacc cccttccgac agggacggaa
     1741 atggtggaga agcgacccct gcgggagagg caccggaccc ctccccaatg gcatctcgcc
     1801 tacgtgggag acgggagccc cctgtggccg actccactac ctcgcaggca ttccccctcc
     1861 gcgcaggagg aaacggacag cttcaatact ggccgttctc ctcttctgac ctttacaact
     1921 ggaaaaataa taacccttct ttttctgaag atccaggtaa actgacagct ctgatcgagt
     1981 ctgttctcat cacccatcag cccacctggg acgactgtca gcagctgttg gggactctgc
     2041 tgaccggaga agaaaaacaa cgggtgctct tagaggctag aaaggcggtg cggggcgatg
     2101 atgggcgccc cactcaactg cccaatgaag tcgatgccgc ttttcccctc gagcgcccag
     2161 actgggatta caccacccag gcaggtagga accacctagt ccactatcgc cagttgctcc
     2221 tagcgggtct ccaaaacgcg ggcagaagcc ccaccaattt ggccaaggta aaaggaataa
     2281 cacaagggcc caatgagtct ccctcggcct tcctagagag acttaaggaa gcctatcgca
     2341 ggtacactcc ttatgaccct gaggacccag ggcaagaaac taatgtgtct atgtctttca
     2401 tttggcagtc tgccccagac attgggagaa agttagagag gttagaagat ttaaaaaaca
     2461 agacgcttgg agatttggtt agagaggcag aaaagatctt taataaacga gaaaccccgg
     2521 aagaaagaga ggaacgtatc aggagagaaa cagaggaaaa agaagaacgc cgtaggacag
     2581 aggatgagca gaaagagaaa gaaagagatc gtaggagaca tagagagatg agcaagctat
     2641 tggccactgt cgttagtgga cagaaacagg atagacaggg aggagaacga aggaggtccc
     2701 aactcgatcg cgaccagtgt gcctactgca aagaaaaggg gcactgggct aaagattgtc
     2761 ccaagaaacc acgaggacct cggggaccaa gaccgcaacc ggtcgccacc atggcggaag
     2821 gctccgtcgc caggcagcct gacctcttga cctgcgaaca tgaagagatc cacctcgccg
     2881 gctcgatcca gccgcatggc gcgcttctgg tcgtcagcga acatgatcat cgcgtcatcc
     2941 aggccagcgc caacgccgcg gaatttctga atctcggaag cgtactcggc gttccgctcg
     3001 ccgagatcga cggcgatctg ttgatcaaga tcctgccgca tctcgatccc accgccgaag
     3061 gcatgccggt cgcggtgcgc tgccggatcg gcaatccctc tacggagtac tgcggtctga
     3121 tgcatcggcc tccggaaggc gggctgatca tcgaactcga acgtgccggc ccgtcgatcg
     3181 atctgtcagg cacgctggcg ccggcgctgg agcggatccg cacggcgggt tcactgcgcg
     3241 cgctgtgcga tgacaccgtg ctgctgtttc agcagtgcac cggctacgac cgggtgatgg
     3301 tgtatcgttt cgatgagcaa ggccacggcc tggtattctc cgagtgccat gtgcctgggc
     3361 tcgaatccta tttcggcaac cgctatccgt cgtcgactgt cccgcagatg gcgcggcagc
     3421 tgtacgtgcg gcagcgcgtc cgcgtgctgg tcgacgtcac ctatcagccg gtgccgctgg
     3481 agccgcggct gtcgccgctg accgggcgcg atctcgacat gtcgggctgc ttcctgcgct
     3541 cgatgtcgcc gtgccatctg cagttcctga aggacatggg cgtgcgcgcc accctggcgg
     3601 tgtcgctggt ggtcggcggc aagctgtggg gcctggttgt ctgtcaccat tatctgccgc
     3661 gcttcatccg tttcgagctg cgggcgatct gcaaacggct cgccgaaagg atcgcgacgc
     3721 ggatcaccgc gcttgagagc taagcggccg cgactctaga tcataatcag ccataccaca
     3781 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3841 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3901 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3961 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     4021 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4081 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4141 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4201 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4261 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4321 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4381 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4441 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4501 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4561 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4621 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4681 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4741 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4801 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4861 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4921 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4981 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5041 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5101 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     5161 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5221 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5281 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5341 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5401 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5461 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     5521 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5581 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5641 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5701 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5761 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5821 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5881 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5941 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     6001 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     6061 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     6121 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     6181 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     6241 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6301 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6481 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7021 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
