LOCUS       .                       8743 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    227..352
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    845..1078
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1263..1307
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1456..1497
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1574..1690
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             2431..4080
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(4124..4245)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    4359..4947
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5034..5267
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5339..5473
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5500..5635
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5656..5674)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5684..5700)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5842..6297
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6323..6427
                     /label="AmpR promoter"
     CDS             6428..7285
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      7459..8047
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8335..8356
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8371..8401
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8409..8425
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     8433..8449
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8470..8488
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8516..8742
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
       61 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      361 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      421 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      481 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      541 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      601 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      661 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
      721 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
      781 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
      841 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
      901 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
      961 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1021 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1081 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1141 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1201 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1261 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1321 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1381 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1441 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     1501 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     1561 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     1621 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat
     1681 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
     1741 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1801 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1861 cacttggcag tacatcaagt gtatcatatg actagcaaaa taggctgtcc ccagtgcaag
     1921 tgcaggtgcc agaacatttc tctatcgata ggtacctctg ccggctgtga gttgcgcagc
     1981 ggccaagcac cattcccccg tgccgcagtg gtacgcgcca ctccggggct gcacgagcgg
     2041 gccaccgccg tgccaggtga atggaagtcc cgcaggccgg aagtggacga gcctactctc
     2101 ccgggcgcgg gggggcgcaa gagcgcgcag catcttcatt gaggaacccg ggcggcgaac
     2161 atggagttcc atgtgcgtct tatgtaaaga gagcgacggg cgtctccacc aattgatgag
     2221 cgtagctctc aggttccgcc ccgccagtat gcaaatgagg tggcactccg accaatggcg
     2281 cgcgcagggg cgggctcagc gcgcgcggtc acgttttcca ctatgtgaca gcggagggcg
     2341 acgcggcggc agcggcgcta ctgggactag cggtctcgag atctgcgatc taagtaagct
     2401 tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc
     2461 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
     2521 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
     2581 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
     2641 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     2701 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     2761 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
     2821 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
     2881 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     2941 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
     3001 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
     3061 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
     3121 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     3181 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     3241 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
     3301 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     3361 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
     3421 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
     3481 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     3541 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
     3601 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     3661 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     3721 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
     3781 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca
     3841 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     3901 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
     3961 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     4021 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg
     4081 taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag
     4141 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     4201 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4261 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     4321 ctctacaaat gtggtaaaat cgataaggat ccgtcgacaa tcaacctctg gattacaaaa
     4381 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4441 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4501 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4561 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4621 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4681 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4741 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4801 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4861 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4921 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
     4981 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5041 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     5101 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5161 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5221 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     5281 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5341 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5401 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5461 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     5521 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5581 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     5641 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     5701 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5761 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5821 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5881 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5941 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6001 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6061 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6121 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6181 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6241 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     6301 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6361 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6421 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6481 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6541 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6601 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6661 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6721 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6781 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6841 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6901 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6961 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7021 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7081 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7141 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7201 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7261 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7321 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7381 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7441 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7501 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7561 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7621 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7681 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7741 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7801 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7861 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7921 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7981 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8041 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8101 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8161 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8221 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     8281 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     8341 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     8401 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     8461 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     8521 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     8581 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     8641 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     8701 tgcagagata ttgtatttaa gtgcctagct cgatacataa acg
//
