LOCUS       40924_1864        6145 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector LUCTRAP-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Calderon-Villalobos LI, Kuhnle C, Li H, Rosso M, Weisshaar B, 
            Schwechheimer C.
  TITLE     LucTrap vectors are tools to generate luciferase fusions for the 
            quantification of transcript and protein abundance in vivo
  JOURNAL   Plant Physiol. 141 (1), 3-14 (2006)
  PUBMED    16684932
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Calderon LIA. V, Kuhnle C, Dohmann EMN., Li H, Schwechheimer C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2005) Developmental Genetics, Centre for Plant 
            Molecular Biology, Auf der Morgenstelle 5, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 6145)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6145)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006"; volume: "141"; issue: "1"; pages: "3-14"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-FEB-2005) Developmental Genetics, Centre for Plant Molecular 
            Biology, Auf der Morgenstelle 5, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             320..1132
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYGLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1236..1824
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1915..1939)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2125..3774
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="VEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3932..3948)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3974..3992)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3999..4015)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4262..4441
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4475..5266
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      5309..5560
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(5584..5606)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(join(5739..6145,1..29))
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 agcccgctcg acggcgggct gttggatggg gatcgcctga atcgccccat catccagcca
       61 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt
      121 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt
      181 caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg
      241 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      301 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct ccaggccgcg
      361 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      421 gcaatcaggt gcgacaatct atggattgta tgggaagccc gatgcgccag agttgtttct
      481 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      541 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      601 atggttactc accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc
      661 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
      721 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
      781 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
      841 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
      901 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
      961 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1021 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1081 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     1141 attggttaat tggttgtaac catgaccaaa atcccttaac gtgagttttc gttccactga
     1201 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1261 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1321 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1381 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1441 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1501 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1561 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1621 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1681 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1741 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1801 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1861 ttttgctggc cttttgctca catgagatct caaacaaaca catacagcga cttagtttac
     1921 ccgccaatat atcctgtcaa ggcctgtcga aatcggactg ggaaccggta tttttgttcg
     1981 gtaaaatcac acatgaaaac atatattcag atccaagtcc acaaggaaaa ttgattgtac
     2041 tctttactta aatttactta tgaatcctgc taattgaatg atacaaattt actaatttgc
     2101 aggttatatg caggttatat gcaggtcgaa gacgccaaaa acataaagaa aggcccggcg
     2161 ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
     2221 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact
     2281 tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
     2341 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
     2401 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
     2461 gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg
     2521 ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg
     2581 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     2641 cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt
     2701 gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat
     2761 agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     2821 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     2881 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     2941 tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc
     3001 tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     3061 gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat
     3121 ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca
     3181 cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag
     3241 gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg
     3301 agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt
     3361 gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc
     3421 ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct
     3481 gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt
     3541 cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg
     3601 atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg
     3661 cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca
     3721 agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc
     3781 tagatcactg gattttagga attagaaatt ttattgatag aagtatttta caaatacaaa
     3841 tacatactaa gggtttctta tatgctcaac acatgagcga aaccctataa gaaccctaat
     3901 tcccttatct ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg
     3961 gtacccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
     4021 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4081 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4141 ctgaatggcg aatggcgcga aattgtaaac gttaatgtta tcgatacatg agaattaagg
     4201 gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac
     4261 agaaccgcaa cgttgaagga gccactcagc cgcgggtttc tggagtttaa tgagctaagc
     4321 acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc
     4381 aaatatttct tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt
     4441 agagtctcat attcactctc aactcgatcg aggcatgatt gaacaagatg gattgcacgc
     4501 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4561 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4621 caagaccgac ctgtccggtg ccctgaatga actccaagac gaggcagcgc ggctatcgtg
     4681 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4741 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4801 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4861 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgaggacgta ctcggatgga
     4921 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4981 actgttcgcc aggctcaagg cgcggatgcc cgacggcgag gatctcgtcg tgacccaggg
     5041 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5101 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5161 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5221 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     5281 gggttcggac tctagctaga gtcaagcaga tcgttcaaac atttggcaat aaagtttctt
     5341 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     5401 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg ttttttgatt
     5461 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     5521 gataaattat cgcgcgcggt gtcatctatg ttactagatc gaccggcatg caagctgata
     5581 acgttacacc acaatatatc ctgccaagat ctaattccgg ggatcggaaa tccagaagcc
     5641 cgagaggttg ccgcctttcg ggctttttct ttttcaaaaa aaaaaattta taaaacgatc
     5701 tgttgcggcc ggccgccggg ttgtgggcaa aggcgctggc gctcgacggt gggcaaccgc
     5761 ttgcggttgt ccacgggcgg agccggtgcg cgtagcgcat tgtccacaag ccaagggcga
     5821 ccaataattg atatatatat tcataattga aaagctaatt gaacatacta cttgctgtaa
     5881 ctacttgccg gagcgagggg tgtttgcaag ctgttgatct gaaagggcta ttagcgttct
     5941 cacgtgcctt tttgattagc gatttcacgt gaccttatta gcgatttcac gtactccgat
     6001 tagcgatttc acgtaccctg attagcgatt tcacgtggat agtttttgga gcgggccgga
     6061 aagccccgtg aatcaaggct ttgcggggca ttagcggttt cacgtggata actaccctct
     6121 atccacaggc ttccggggat aaaaa
//
