LOCUS       40924_1859        6149 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector LUCTRAP-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Calderon-Villalobos LI, Kuhnle C, Li H, Rosso M, Weisshaar B, 
            Schwechheimer C.
  TITLE     LucTrap vectors are tools to generate luciferase fusions for the 
            quantification of transcript and protein abundance in vivo
  JOURNAL   Plant Physiol. 141 (1), 3-14 (2006)
  PUBMED    16684932
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Calderon LIA. V, Kuhnle C, Dohmann EMN., Li H, Schwechheimer C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2005) Developmental Genetics, Centre for Plant 
            Molecular Biology, Auf der Morgenstelle 5, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 6149)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6149)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2006"; volume: "141"; issue: "1"; pages: "3-14"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-FEB-2005) Developmental Genetics, Centre for Plant Molecular 
            Biology, Auf der Morgenstelle 5, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             320..1132
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYGLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1236..1824
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1915..1939)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2129..3778
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3936..3952)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3978..3996)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4003..4019)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4266..4445
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4479..5270
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      5313..5564
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(5588..5610)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(join(5743..6149,1..29))
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 agcccgctcg acggcgggct gttggatggg gatcgcctga atcgccccat catccagcca
       61 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt
      121 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt
      181 caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg
      241 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      301 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct ccaggccgcg
      361 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      421 gcaatcaggt gcgacaatct atggattgta tgggaagccc gatgcgccag agttgtttct
      481 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      541 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      601 atggttactc accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc
      661 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
      721 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
      781 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
      841 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
      901 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
      961 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1021 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1081 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     1141 attggttaat tggttgtaac catgaccaaa atcccttaac gtgagttttc gttccactga
     1201 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1261 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1321 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1381 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1441 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1501 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1561 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1621 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1681 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1741 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1801 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1861 ttttgctggc cttttgctca catgagatct caaacaaaca catacagcga cttagtttac
     1921 ccgccaatat atcctgtcaa ggcctgtcga aatcggactg ggaaccggta tttttgttcg
     1981 gtaaaatcac acatgaaaac atatattcag atccaagtcc acaaggaaaa ttgattgtac
     2041 tctttactta aatttactta tgaatcctgc taattgaatg atacaaattt actaatttgc
     2101 aggttatatg caggttatat gcaggtccat ggaagacgcc aaaaacataa agaaaggccc
     2161 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
     2221 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
     2281 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
     2341 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
     2401 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
     2461 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
     2521 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
     2581 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
     2641 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
     2701 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc
     2761 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat
     2821 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     2881 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     2941 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct
     3001 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     3061 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt
     3121 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat
     3181 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     3241 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg
     3301 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     3361 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     3421 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
     3481 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
     3541 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     3601 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     3661 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     3721 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta
     3781 attctagatc actggatttt aggaattaga aattttattg atagaagtat tttacaaata
     3841 caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct ataagaaccc
     3901 taattccctt atctggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
     3961 cccggtaccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
     4021 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4081 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4141 cagcctgaat ggcgaatggc gcgaaattgt aaacgttaat gttatcgata catgagaatt
     4201 aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac
     4261 tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt ttaatgagct
     4321 aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc
     4381 tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc
     4441 aattagagtc tcatattcac tctcaactcg atcgaggcat gattgaacaa gatggattgc
     4501 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4561 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4621 ttgtcaagac cgacctgtcc ggtgccctga atgaactcca agacgaggca gcgcggctat
     4681 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4741 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4801 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     4861 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagga cgtactcgga
     4921 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     4981 ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc
     5041 agggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5101 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5161 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5221 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5281 tctggggttc ggactctagc tagagtcaag cagatcgttc aaacatttgg caataaagtt
     5341 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     5401 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggtttttt
     5461 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     5521 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgaccgg catgcaagct
     5581 gataacgtta caccacaata tatcctgcca agatctaatt ccggggatcg gaaatccaga
     5641 agcccgagag gttgccgcct ttcgggcttt ttctttttca aaaaaaaaaa tttataaaac
     5701 gatctgttgc ggccggccgc cgggttgtgg gcaaaggcgc tggcgctcga cggtgggcaa
     5761 ccgcttgcgg ttgtccacgg gcggagccgg tgcgcgtagc gcattgtcca caagccaagg
     5821 gcgaccaata attgatatat atattcataa ttgaaaagct aattgaacat actacttgct
     5881 gtaactactt gccggagcga ggggtgtttg caagctgttg atctgaaagg gctattagcg
     5941 ttctcacgtg cctttttgat tagcgatttc acgtgacctt attagcgatt tcacgtactc
     6001 cgattagcga tttcacgtac cctgattagc gatttcacgt ggatagtttt tggagcgggc
     6061 cggaaagccc cgtgaatcaa ggctttgcgg ggcattagcg gtttcacgtg gataactacc
     6121 ctctatccac aggcttccgg ggataaaaa
//
