LOCUS       LSL-Cas9-Rosa26T       20597 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Zhang lab vector with positive and negative selection markers, for 
            targeting Cre-dependent Cas9 and EGFP expression to the mouse Rosa26
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    LSL-Cas9-Rosa26TV
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20597)
  AUTHORS   Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, 
            Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, 
            Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A,
            Feng G, Sharp PA, Zhang F.
  TITLE     CRISPR-Cas9 knockin mice for genome editing and cancer modeling.
  JOURNAL   Cell 2014;159:440-55.
  PUBMED    25263330
REFERENCE   2  (bases 1 to 20597)
  AUTHORS   Zhang Lab / Addgene #61408
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 20597)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "2014"; volume: "159"; pages: "440-55"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     A number of sequence corrections were made based on Addgene 
            sequencing results and comparisons with GenBank data.
FEATURES             Location/Qualifiers
     source          1..20597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1085
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1763
                     /note="chicken beta-actin promoter"
     intron          1765..2782
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     protein_bind    2858..2891
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     protein_bind    2915..2948
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3115..3249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3369..3503
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     polyA_signal    3623..3757
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    3786..3819
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             3883..3885
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             3886..3951
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=three tandem FLAG
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3958..3978
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             4003..8103
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             8104..8151
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=bipartite nuclear localization signal from
                     nucl
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8161..8217
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=2A peptide from porcine teschovirus-1
                     polyprotein
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8218..8931
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    8985..9573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    9594..9801
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9809..9842
                     /label=attB
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et
                     al., 2000)"
     promoter        9864..10363
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    10383..10416
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             10423..11223
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    11236..11697
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation
                     signal"
     misc_feature    11763..16103
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        16152..16651
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             16669..16671
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             16678..17247
                     /label=DTA
                     /note="diphteria toxin Fragment A"
     intron          17324..17389
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    17463..17687
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(17749..17767)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(17836..17866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(17881..17902)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(18190..18778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(18952..19809)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(19810..19914)
                     /label=AmpR promoter
     rep_origin      complement(19940..20395)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     20537..20553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        20563..20581
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     1081 tctagcctta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
     1621 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg
     1801 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     1861 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     1921 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     1981 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     2041 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     2101 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     2161 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     2221 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     2281 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     2341 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     2401 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     2461 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     2521 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     2581 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     2641 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     2701 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     2761 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     2821 ttttggcaaa gaattgattt gataccgcgg gccctaagaa gttcctattc tctagaaagt
     2881 ataggaactt cgtcgacatt taaatcattt aaatataact tcgtataatg tatgctatac
     2941 gaagttattc gcgatgaata aatgaaagct tgcagatctg cgactctaga ggatctgcga
     3001 ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3061 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3241 gtctggatct gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac
     3301 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3361 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3421 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3481 atgtatctta tcatgtctgg atctgcgact ctagaggatc ataatcagcc ataccacatt
     3541 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3601 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3661 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3721 gtccaaactc atcaatgtat cttatcatgt ctggatcccc atcaagctga tccggaaccc
     3781 ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg acctgcagcc
     3841 caagctagat cgaattcggc cggccttcga acacgtgcca ccatggacta taaggaccac
     3901 gacggagact acaaggatca tgatattgat tacaaagacg atgacgataa gatggcccca
     3961 aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtacagcatc
     4021 ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
     4081 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
     4141 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
     4201 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
     4261 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
     4321 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
     4381 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
     4441 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
     4501 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
     4561 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
     4621 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
     4681 cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc
     4741 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
     4801 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
     4861 gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga
     4921 gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag
     4981 caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac
     5041 aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc
     5101 agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag
     5161 gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac
     5221 ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa
     5281 gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc
     5341 atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga
     5401 aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc
     5461 gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg
     5521 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg
     5581 aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc
     5641 atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac
     5701 tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc
     5761 aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg
     5821 gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag
     5881 gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg
     5941 atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc
     6001 aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc
     6061 ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac
     6121 atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg
     6181 gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc
     6241 gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac
     6301 cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc
     6361 atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag
     6421 aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa
     6481 ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg
     6541 aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc
     6601 gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg
     6661 aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc
     6721 ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc
     6781 acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac
     6841 aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg
     6901 aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc
     6961 tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag
     7021 ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag
     7081 gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag
     7141 accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc
     7201 gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg
     7261 agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa
     7321 gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac
     7381 cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc
     7441 aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc
     7501 atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac
     7561 aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa
     7621 aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc
     7681 ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc
     7741 tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac
     7801 gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg
     7861 gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag
     7921 aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt
     7981 gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg
     8041 atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc
     8101 gacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggaagcgga
     8161 gccactaact tctccctgtt gaaacaagca ggggatgtcg aagagaatcc cgggccagtg
     8221 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     8281 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     8341 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     8401 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     8461 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     8521 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     8581 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     8641 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     8701 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     8761 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     8821 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     8881 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaacgcgt
     8941 atgcatggcc ggccctgcag gaattcgata tcaagcttat cgataatcaa cctctggatt
     9001 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     9061 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     9121 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     9181 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     9241 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     9301 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     9361 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     9421 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     9481 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     9541 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgactgtgcc
     9601 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     9661 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     9721 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     9781 caatagcagg catgctgggg aactagtggt gccagggcgt gcccttgggc tccccgggcg
     9841 cggcggccgc atcgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     9901 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     9961 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
    10021 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
    10081 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
    10141 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
    10201 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
    10261 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
    10321 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca atcgccgcta
    10381 gcgaagttcc tattctctag aaagtatagg aacttcgcca ccatgggatc ggccattgaa
    10441 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
    10501 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
    10561 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
    10621 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
    10681 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
    10741 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
    10801 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
    10861 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
    10921 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat
    10981 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
    11041 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
    11101 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
    11161 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
    11221 ttctgagggg atccgctgta agtctgcaga aattgatgat ctattaaaca ataaagatgt
    11281 ccactaaaat ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct
    11341 acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg
    11401 cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat
    11461 cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta
    11521 tagatctata gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct
    11581 aagtactgtg gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc
    11641 tgttccacat acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaaagc
    11701 ttgcagaaga tctccccaac tggggtaacc tttgagttct ctcagttggg ggggcgcgcc
    11761 ggctagaaga tgggcgggag tcttctgggc aggcttaaag gctaacctgg tgtgtgggcg
    11821 ttgtcctgca ggggaattga acaggtgtaa aattggaggg acaagacttc ccacagattt
    11881 tcggttttgt cgggaagttt tttaataggg gcaaataagg aaaatgggag gataggtagt
    11941 catctggggt tttatgcagc aaaactacag gttattattg cttgtgatcc gcctcggagt
    12001 attttccatc gaggtagatt aaagacatgc tcacccgagt tttatactct cctgcttgag
    12061 atccttacta cagtatgaaa ttacagtgtc gcgagttaga ctatgtaagc agaattttaa
    12121 tcatttttaa agagcccagt acttcatatc catttctccc gctccttctg cagccttatc
    12181 aaaaggtatt ttagaacact cattttagcc ccattttcat ttattatact ggcttatcca
    12241 acccctagac agagcattgg cattttccct ttcctgatct tagaagtctg atgactcatg
    12301 aaaccagaca gattagttac atacaccaca aatcgaggct gtagctgggg cctcaacact
    12361 gcagttcttt tataactcct tagtacactt tttgttgatc ctttgccttg atccttaatt
    12421 ttcagtgtct atcacctctc ccgtcaggtg gtgttccaca tttgggccta ttctcagtcc
    12481 agggagtttt acaacaatag atgtattgag aatccaacct aaagcttaac tttccactcc
    12541 catgaatgcc tctctccttt ttctccattt ataaactgag ctattaacca ttaatggttt
    12601 ccaggtggat gtctcctccc ccaatattac ctgatgtatc ttacatattg ccaggctgat
    12661 attttaagac attaaaaggt atatttcatt attgagccac atggtattga ttactgctta
    12721 ctaaaatttt gtcattgtac acatctgtaa aaggtggttc cttttggaat gcaaagttca
    12781 ggtgtttgtt gtctttcctg acctaaggtc ttgtgagctt gtattttttc tatttaagca
    12841 gtgctttctc ttggactggc ttgactcatg gcattctaca cgttattgct ggtctaaatg
    12901 tgattttgcc aagcttcttc aggacctata attttgcttg acttgtagcc aaacacaagt
    12961 aaaatgatta agcaacaaat gtatttgtga agcttggttt ttaggttgtt gtgttgtgtg
    13021 tgcttgtgct ctataataat actatccagg ggctggagag gtggctcgga gttcaagagc
    13081 acagactgct cttccagaag tcctgagttc aattcccagc aaccacatgg tggctcacaa
    13141 ccatctgtaa tgggatctga tgccctcttc tggtgtgtct gaagaccaca agtgtattca
    13201 cattaaataa ataaatcctc cttcttcttc tttttttttt ttttaaagag aatactgtct
    13261 ccagtagaat ttactgaagt aatgaaatac tttgtgtttg ttccaatatg gtagccaata
    13321 atcaaattac tctttaagca ctggaaatgt taccaaggaa ctaattttta tttgaagtgt
    13381 aactgtggac agaggagcca taactgcaga cttgtgggat acagaagacc aatgcagact
    13441 ttaatgtctt ttctcttaca ctaagcaata aagaaataaa aattgaactt ctagtatcct
    13501 atttgtttaa actgctagct ttacttaact tttgtgcttc atctatacaa agctgaaagc
    13561 taagtctgca gccattacta aacatgaaag caagtaatga taattttgga tttcaaaaat
    13621 gtagggccag agtttagcca gccagtggtg gtgcttgcct ttatgccttt aatcccagca
    13681 ctctggaggc agagacaggc agatctctga gtttgagccc agcctggtct acacatcaag
    13741 ttctatctag gatagccagg aatacacaca gaaaccctgt tggggagggg ggctctgaga
    13801 tttcataaaa ttataattga agcattccct aatgagccac tatggatgtg gctaaatccg
    13861 tctacctttc tgatgagatt tgggtattat tttttctgtc tctgctgttg gttgggtctt
    13921 ttgacactgt gggctttctt taaagcctcc ttcctgccat gtggtctctt gtttgctact
    13981 aacttcccat ggcttaaatg gcatggcttt ttgccttcta agggcagctg ctgagatttg
    14041 cagcctgatt tccagggtgg ggttgggaaa tctttcaaac actaaaattg tcctttaatt
    14101 ttttttttaa aaaatgggtt atataataaa cctcataaaa tagttatgag gagtgaggtg
    14161 gactaatatt aaatgagtcc ctcccctata aaagagctat taaggctttt tgtcttatac
    14221 ttaacttttt ttttaaatgt ggtatcttta gaaccaaggg tcttagagtt ttagtataca
    14281 gaaactgttg catcgcttaa tcagattttc tagtttcaaa tccagagaat ccaaattctt
    14341 cacagccaaa gtcaaattaa gaatttctga cttttaatgt taatttgctt actgtgaata
    14401 taaaaatgat agcttttcct gaggcagggt ctcactatgt atctctgcct gatctgcaac
    14461 aagatatgta gactaaagtt ctgcctgctt ttgtctcctg aatactaagg ttaaaatgta
    14521 gtaatacttt tggaacttgc aggtcagatt cttttatagg ggacacacta agggagcttg
    14581 ggtgatagtt ggtaaaatgt gtttcaagtg atgaaaactt gaattattat caccgcaacc
    14641 tactttttaa aaaaaaaagc caggcctgtt agagcatgct taagggatcc ctaggacttg
    14701 ctgagcacac aagagtagtt acttggcagg ctcctggtga gagcatattt caaaaaacaa
    14761 ggcagacaac caagaaacta cagttaaggt tacctgtctt taaaccatct gcatatacac
    14821 agggatatta aaatattcca aataatattt cattcaagtt ttcccccatc aaattgggac
    14881 atggatttct ccggtgaata ggcagagttg gaaactaaac aaatgttggt tttgtgattt
    14941 gtgaaattgt tttcaagtga tagttaaagc ccatgagata cagaacaaag ctgctatttc
    15001 gaggtctctt ggtttatact cagaagcact tctttgggtt tccctgcact atcctgatca
    15061 tgtgctaggc ctaccttagg ctgattgttg ttcaaataaa cttaagtttc ctgtcaggtg
    15121 atgtcatatg atttcatata tcaaggcaaa acatgttata tatgttaaac atttgtactt
    15181 aatgtgaaag ttaggtcttt gtgggtttga tttttaattt tcaaaacctg agctaaataa
    15241 gtcattttta catgtcttac atttggtgga attgtataat tgtggtttgc aggcaagact
    15301 ctctgaccta gtaaccctac ctatagagca ctttgctggg tcacaagtct aggagtcaag
    15361 catttcacct tgaagttgag acgttttgtt agtgtatact agtttatatg ttggaggaca
    15421 tgtttatcca gaagatattc aggactattt ttgactgggc taaggaattg attctgatta
    15481 gcactgttag tgagcattga gtggccttta ggcttgaatt ggagtcactt gtatatctca
    15541 aataatgctg gcctttttta aaaagccctt gttctttatc accctgtttt ctacataatt
    15601 tttgttcaaa gaaatacttg tttggatctc cttttgacaa caatagcatg ttttcaagcc
    15661 atattttttt tccttttttt tttttttttt ggtttttcga gacagggttt ctctgtatag
    15721 ccctggctgt cctggaactc actttgtaga ccaggctggc ctcgaactca gaaatccgcc
    15781 tgcctctgcc tcctgagtgc cgggattaaa ggcgtgcacc accacgcctg gctaagttgg
    15841 atattttgtt atataactat aaccaatact aactccactg ggtggatttt taattcagtc
    15901 agtagtctta agtggtcttt attggccctt cattaaaatc tactgttcac tctaacagag
    15961 gctgttggta ctagtggcac ttaagcaact tcctacggat atactagcag attaagggtc
    16021 agggatagaa actagtctag cgttttgtat acctaccagc tttatactac cttgttctga
    16081 tagaaatatt tcaggacatc tagcttatcg atccgtcgac ggtatcgata agcttgatat
    16141 cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
    16201 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
    16261 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
    16321 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
    16381 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
    16441 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
    16501 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
    16561 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    16621 gttctcctct tcctcatctc cgggcctttc gacctgcagg tcctcgccat ggatcctgat
    16681 gatgttgtta ttcttctaat cttttgtatg gaaaactttt cttcgtacca cgggactaaa
    16741 cctggttatg tagattccat tcaaaaaggt atacaaaagc caaaatctgg tacacaagga
    16801 aattatgacg atgattggaa agggttttat agtaccgaca ataaatacga cgctgcggga
    16861 tactctgtag ataatgaaaa cccgctctct ggaaaagctg gaggcgtggt caaagtgacg
    16921 tatccaggac tgacgaaggt tctcgcacta aaagtggata atgccgaaac tattaagaaa
    16981 gagttaggtt taagtctcac tgaaccgttg atggagcaag tcggaacgga agagtttatc
    17041 aaaaggttcg gtgatggtgc ttcgcgtgta gtgctcagcc ttcccttcgc tgaggggagt
    17101 tctagcgttg aatatattaa taactgggaa caggcgaaag cgttaagcgt agaacttgag
    17161 attaattttg aaacccgtgg aaaacgtggc caagatgcga tgtatgagta tatggctcaa
    17221 gcctgtgcag gaaatcgtgt caggcgatct ctttgtgaag gaaccttact tctgtggtgt
    17281 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
    17341 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
    17401 tggaactgat gaatgggagc agtggtggaa tgcagatcct agagctcgct gatcagcctc
    17461 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
    17521 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    17581 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
    17641 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
    17701 aagaaccagc tggggctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
    17761 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    17821 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    17881 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    17941 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    18001 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    18061 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    18121 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    18181 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    18241 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    18301 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    18361 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    18421 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    18481 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    18541 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    18601 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    18661 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    18721 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    18781 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    18841 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    18901 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    18961 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    19021 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    19081 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    19141 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    19201 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    19261 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    19321 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    19381 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    19441 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    19501 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    19561 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    19621 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    19681 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    19741 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    19801 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    19861 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    19921 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
    19981 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
    20041 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
    20101 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
    20161 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
    20221 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
    20281 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
    20341 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
    20401 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    20461 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    20521 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
    20581 ggcgaattgg agctcca
//
