LOCUS       40924_1834        7484 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector LNXCO3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7484)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7484)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             146..733
                     /label=5' LTR
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
     misc_feature    796..995
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1196..1612
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     CDS             1656..2447
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    complement(3033..3080)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    3470..3489
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3513..3532
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    complement(3556..3575)
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(3750..3953)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(3954..4333)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             4530..5123
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    5334..5474
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5660..6248)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6422..7279)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7280..7384)
                     /label=AmpR promoter
ORIGIN
        1 gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
       61 tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
      121 ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
      181 aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
      241 caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
      301 tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
      361 tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
      421 cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
      481 aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
      541 cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
      601 cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
      661 gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
      721 ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
      781 ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
      841 tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
      901 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
      961 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
     1021 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     1081 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
     1141 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
     1201 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
     1261 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
     1321 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
     1381 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
     1441 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
     1501 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
     1561 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
     1621 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     1681 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     1741 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     1801 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     1861 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     1921 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     1981 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     2041 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     2101 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     2161 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     2221 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     2281 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     2341 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     2401 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     2461 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     2521 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     2581 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
     2641 gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
     2701 atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
     2761 ggagtgggga ggcacgatgg ccgctttggt cgaggcggat cctagccccc acgaaccata
     2821 aaccattccc catgggggac cccgtcccta acccacgggg ccagtggcta tggcagggcc
     2881 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
     2941 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
     3001 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
     3061 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
     3121 ttcagttctt aagaattgcg gccgcgatcg cgttaacaga tctggccgca agcttggttg
     3181 gaatctaaaa tacacaaaca attaggatcc ctgaggagca gttctttgat ttgcaccacc
     3241 aggagcctca aatttttcaa taaattcacc tgactgcaca ttaggacttt ggctttgagc
     3301 ttcccacctc tcagttactt gctgaatact gtctgcttca tcaatattat cataggtgtg
     3361 cccaaatgat atttgcaacc cttccctgtt ggctacttgt ctcaccattg taggcctaat
     3421 gggagacgga tccgtagttt aacacattat acactagggt cgactgtgga attgtgagcg
     3481 ctcacaattc cacaacctag ggtcgactgt ggaattgtga gcgctcacaa ttccacaacc
     3541 tagggtcgac tgtggaattg tgagcgctca caattccaca acctagtaaa ttttatattt
     3601 accttagagc tttaaatctc tgtaggtagt ttgtccaagc ttagatcaac aagcttgggg
     3661 ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt
     3721 ctccaggcga tctgacggtt cactaaacga gctctgctta tatagacctc ccaccgtaca
     3781 tgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
     3841 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
     3901 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
     3961 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
     4021 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
     4081 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
     4141 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
     4201 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
     4261 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactagtc
     4321 aataatcaat gtcaacatgg cggtaatgtt ggacatgagc caatataaat gtacatatta
     4381 tgatatggat acaacgtatg caatggccaa tagccaatat tgatttatgc tatataacca
     4441 atgaataata tggctaatgg ccggatcgtg gagctcgcgc gaacatcgat aaaataaaag
     4501 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     4561 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     4621 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     4681 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
     4741 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     4801 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     4861 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     4921 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     4981 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     5041 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     5101 cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg
     5161 gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg tgatgacggt
     5221 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     5281 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     5341 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     5401 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     5461 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5521 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5581 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5641 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5701 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5761 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5821 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5881 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5941 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6001 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6061 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     6121 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6181 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6241 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6301 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6361 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6421 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6481 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6541 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6601 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6661 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6721 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6781 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6841 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6901 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6961 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7021 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7081 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7141 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7201 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7261 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7321 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7381 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     7441 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaa
//
