LOCUS       40924_1774       10526 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector LIC-pKLAC-LC2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Dortay H, Akula UM, Westphal C, Sittig M, Mueller-Roeber B.
  TITLE     High-Throughput Protein Expression Using a Combination of 
            Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein 
            (IFP) Detection
  JOURNAL   PLoS ONE 6 (4), E18900 (2011)
  PUBMED    21541323
REFERENCE   2  (bases 1 to 10526)
  AUTHORS   Dortay H, Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2011) Molecular Biology, University of Potsdam, 
            Germany, Karl-Liebknecht-Str. 24-25, Haus 20, Potsdam, Brandenburg 
            14476, Germany
REFERENCE   3  (bases 1 to 10526)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10526)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "4"; pages: "E18900"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-FEB-2011) Molecular Biology, University of Potsdam, Germany, 
            Karl-Liebknecht-Str. 24-25, Haus 20, Potsdam, Brandenburg 14476, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    14..16
                     /label=start codon
                     /note="start codon"
     misc_feature    26..33
                     /label=PmeI recognition site
                     /note="PmeI recognition site"
     misc_feature    34..700
                     /label=LIC stuffer fragment
                     /note="LIC stuffer fragment"
     misc_feature    701..708
                     /label=PmeI recognition site
                     /note="PmeI recognition site"
     CDS             714..734
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             744..1727
                     /codon_start=1
                     /label=IFP1.4
                     /note="bacteriophytochrome-based monomeric infrared
                     fluorescent protein (Shu et al., 2009)"
                     /translation="MARDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELTGFDRVMLYKFAPDATGEMIAEARREGMQAFLGHRFPASHTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEELGRKLSGQVQRKEAGMDELYK"
     CDS             1734..1751
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     misc_feature    1752..1757
                     /label=stop codon (TAA TAG)
                     /note="stop codon (TAA TAG)"
     terminator      1790..2408
                     /label=LAC4 terminator
                     /note="transcription terminator for Kluyveromyces lactis
                     LAC4"
     promoter        2409..3111
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             3132..4775
                     /codon_start=1
                     /label=amdS
                     /note="acetamidase from Aspergillus nidulans"
                     /translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGIL
                     SEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALAQA
                     RELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRK
                     AGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVG
                     TDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFT
                     KSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPPILRGVE
                     TTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAIPNIKDLL
                     NPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPTAAVRHDQF
                     RYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYDPEAYHGAPV
                     AVQVIGRRLSEERTLAIAEEVGKLLGNVVTP"
     promoter        5414..6070
                     /label=LAC4 promoter (5')
                     /note="5' portion of the Kluyveromyces lactis LAC4
                     promoter"
     promoter        complement(6087..6105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6119..6135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6143..6159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6167..6197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6212..6233)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6521..7109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7283..8140)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8141..8245)
                     /label=AmpR promoter
     rep_origin      8272..8727
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8868..8884
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8898..10426
                     /label=LAC4 promoter (3')
                     /note="3' portion of a modified Kluyveromyces lactis LAC4 
                     promoter"
     protein_bind    10432..10448
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aagcttgaaa aaaatgggtt cttctgttta aacctcagtg tcatcattcg aaagattcga
       61 aatatttccc aaagaaagct catggcccaa ccacaacaca aacaaaggag taacggcagc
      121 atgctcccat gcgggggaaa gcagttttta cagaaatttg ctatggctga cggagaagga
      181 gggctcatac ccaaagctga aaaattctta tgtgaacaag agagggaaag aagtccttgt
      241 actgtggggt attcatcacc cgtctaacag taaggagcaa cagaatatct atcagaatga
      301 aaatgcttat gtctctgtag tgacttcaaa ttataacagg agatttaccc cggaaatagc
      361 agaaagaccc aaagtaagag atcaagctgg gaggatgaac tattactgga ccttgctaaa
      421 acccggagac acaataatat ttgaggcaaa tggaaatcta atagcaccaa ggtatgcttt
      481 cgcactgagt agaggctttg ggtccggcat catcacctca aacgcatcaa tgcatgagtg
      541 taacacgaag tgtcaaacac ccctgggagc tataaacagc agtctccctt tccagaatat
      601 acacccagtc acaataggag agtgcccaaa atacgtcagg agtgccaaat tgaggatggt
      661 tacaggacta aggaacattc cgtccattca atccgctagc gtttaaacag ggcgagaacc
      721 tgtattttca gggcggcggc ggcatggctc gggaccctct gccattcttt ccacctctgt
      781 acctgggcgg ccctgagatt acaaccgaga actgcgagag agagcctatc cacattcctg
      841 ggtccatcca gccacacggg gctctgctca cagctgacgg ccactccgga gaggtgctcc
      901 aagtgtccct gaatgccgct accttcctgg gccaggagcc tactgtgctg cgggggcaga
      961 ccctggctgc cctgctcccc gagcagtggc cagccctgca ggcagccctg cccccaggat
     1021 gtccagatgc cctccaatac agggccaccc tcgactggcc agctgctggg cacctcagcc
     1081 tgactgtgca tcgggtggct gaactcctga tcctggagtt cgaacctacc gaggcctggg
     1141 acagcattgg ccctcacgcc ctgaggaacg ccatgtttgc tctggaaagc gctccaaacc
     1201 tgcgggctct ggccgaagtc gcaacacaaa ctgtgagaga actgactggc ttcgatcggg
     1261 tgatgctgta caaatttgcc cctgacgcca ctggagagat gattgctgag gccagacggg
     1321 agggcatgca ggcttttctg ggccacaggt ttcccgcatc ccacacccct gcacaagcta
     1381 gggccctcta cacaagacac ctgctccggc tgaccgcaga caccagggct gcagcagtgc
     1441 ccctcgaccc cgtgctgaat ccccagacaa atgctcctac acctctgggc ggagctgtcc
     1501 tcagagctac atccccaatg cacatgcagt acctgaggaa tatgggagtg ggctcctccc
     1561 tgagcgtcag cgtcgtggtc ggcggccagc tgtggggact gattgtctgc caccatcaga
     1621 caccctacgt gctgccacca gatctgcgga ccaccctgga ggagctgggg agaaagctgt
     1681 ccgggcaggt gcagaggaaa gaagccggaa tggacgagct gtataaaggt acccaccacc
     1741 atcaccacca ctaataggcg gccgcttaat taaggccttg aatcgagaat ttatacttag
     1801 ataagtatgt acttacaggt atatttctat gagatactga tgtatacatg catgataata
     1861 tttaaacggt tattagtgcc gattgtcttg tgcgataatg acgttcctat caaagcaata
     1921 cacttaccac ctattacatg ggccaagaaa atattttcga acttgtttag aatattagca
     1981 cagagtatat gatgatatcc gttagattat gcatgattca ttcctacaac tttttcgtag
     2041 cataaggatt aattacttgg atgccaataa aaaaaaaaaa catcgagaaa atttcagcat
     2101 gctcagaaac aattgcagtg tatcaaagta aaaaaaagat tttcactaca tgttcctttt
     2161 gaagaaagaa aatcatggaa cattagattt acaaaaattt aaccaccgct gattaacgat
     2221 tagaccgtta agcgcacaac aggttattag tacagagaaa gcattctgtg gtgttgcccc
     2281 ggactttctt ttgcgacata ggtaaatcga ataccatcat actatctttt ccaatgactc
     2341 cctaaagaaa gactcttctt cgatgttgta tacgttggag catagggcaa gaattgtggc
     2401 ttgagatcat ccttttgttg tttccgggtg tacaatatgg acttcctctt ttctggcaac
     2461 caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca tgtaggtggc
     2521 ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta aacaagacta
     2581 caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt agccctagac
     2641 ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca tctattgaag
     2701 taataatagg cgcatgcaac ttcttttctt tttttttctt ttctctctcc cccgttgttg
     2761 tctcaccata tccgcaatga caaaaaaatg atggaagaca ctaaaggaaa aaattaacga
     2821 caaagacagc accaacagat gtcgttgttc cagagctgat gaggggtatc tcgaagcaca
     2881 cgaaactttt tccttccttc attcacgcac actactctct aatgagcaac ggtatacggc
     2941 cttccttcca gttacttgaa tttgaaataa aaaaaagttt gctgtcttgc tatcaagtat
     3001 aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc
     3061 cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca aggaattccg
     3121 gatccgccac catgcctcaa tcctgggaag aactggccgc tgataagcgc gcccgcctcg
     3181 caaaaaccat ccctgatgaa tggaaagtcc agacgctgcc tgcggaagac agcgttattg
     3241 atttcccaaa gaaatcgggg atcctttcag aggccgaact gaagatcaca gaggcttccg
     3301 ctgcggatct tgtgtccaag ctggcggccg gagagttgac ctcggtggaa gttacgctag
     3361 cattctgtaa acgggcagca atcgcccagc agttaacaaa ctgcgcccac gagttcttcc
     3421 ctgacgccgc tctcgcgcag gcaagggaac tcgatgaata ctacgcaaag cacaagagac
     3481 ccgttggtcc actccatggc ctccccatct ctctcaaaga ccagcttcga gtcaagggct
     3541 acgaaacatc aatgggctac atctcatggc taaacaagta cgacgaaggg gactcggttc
     3601 tgacaaccat gctccgcaaa gccggtgccg tcttctacgt caagacctct gtcccgcaga
     3661 ccctgatggt ctgcgagaca gtcaacaaca tcatcgggcg caccgtcaac ccacgcaaca
     3721 agaactggtc gtgcggcggc agttctggtg gtgagggtgc gatcgttggg attcgtggtg
     3781 gcgtcatcgg tgtaggaacg gatatcggtg gctcgattcg agtgccggcc gcgttcaact
     3841 tcctgtacgg tctaaggccg agtcatgggc ggctgccgta tgcaaagatg gcgaacagca
     3901 tggagggtca ggagacggtg cacagcgttg tcgggccgat tacgcactct gttgaggacc
     3961 tccgcctctt caccaaatcc gtcctcggtc aggagccatg gaaatacgac tccaaggtca
     4021 tccccatgcc ctggcgccag tccgagtcgg acattattgc ctccaagatc aagaacggcg
     4081 ggctcaatat cggctactac aacttcgacg gcaatgtcct tccacaccct cctatcctgc
     4141 gcggcgtgga aactaccgtc gccgcactcg ccaaagccgg tcacaccgtg accccgtgga
     4201 cgccatacaa gcacgatttc ggccacgatc tcatctccca tatctacgcg gctgacggca
     4261 gcgccgacgt aatgcgcgat atcagtgcat ccggcgagcc ggcgattcca aatatcaaag
     4321 acctactgaa cccgaacatc aaagctgtta acatgaacga gctctgggac acgcatctcc
     4381 agaagtggaa ttaccagatg gagtaccttg agaaatggcg ggaggctgaa gaaaaggccg
     4441 ggaaggaact ggacgccatc atcgcgccga ttacgcctac cgctgcggta cggcatgacc
     4501 agttccggta ctatgggtat gcctctgtga tcaacctgct ggatttcacg agcgtggttg
     4561 ttccggttac ctttgcggat aagaacatcg ataagaagaa tgagagtttc aaggcggtta
     4621 gtgagcttga tgccctcgtg caggaagagt atgatccgga ggcgtaccat ggggcaccgg
     4681 ttgcagtgca ggttatcgga cggagactca gtgaagagag gacgttggcg attgcagagg
     4741 aagtggggaa gttgctggga aatgtggtga ctccatagcc cggggggggc tcgatcccct
     4801 cgcgagttgg ttcagctgct gcctgaggct ggacgacctc gcggagttct accggcagtg
     4861 caaatccgtc ggcatccagg aaaccagcag cggctatccg cgcatccatg cccccgaact
     4921 gcaggagtgg ggaggcacga tggccgcttt ggtcgatcta gattacgtgg aagaaaggta
     4981 gtaaaagtag tagtataagt agtaaaaaga ggtaaaaaga gaaaaccggc tacatactag
     5041 agaagcacgt acacaaaaac tcataggcac ttcatcatac gacagtttct tgatgcatta
     5101 taatagtgta ttagatattt tcagaaatat gcatagaacc tcctcttgcc tttacttttt
     5161 atacatagaa cattggcaga tttacttaca ctactttgtt tctacgccat ttcttttgtt
     5221 ttcaacactt agacaagttg ttgagaaccg gactactaaa aagcaatgtt cccactgaaa
     5281 atcatgtacc tgcaggataa taacccccta attctgcatc gatccagtat gttttttttt
     5341 ctctactcat ttttacctga agatagagct tctaaaacaa aaaaaatcag tgattacatg
     5401 catattgtgt gttctagtaa ccaaaggaaa ggaacagata gataaaattc cgagactgtc
     5461 aaattaggtt tttttctttt tttttggcgg gagtcagtgg gccgaaatat gttcttggcc
     5521 tagaacttaa tctggtttga tcatgccaat acttgcctga gtgcccgact ttttgcccac
     5581 cctcttgcct tctgtcatcc ttcaaaaccc acctgttttc cagccgtatc ttcgctcgca
     5641 tctacacata ctgtgccata tcttgtgtgt agccggacgt gactatgacc aaaaacaaac
     5701 aaggagaact gttcgccgat ttgtaacact cctgcatcca tccaagtggg tatgcgctat
     5761 gcaatgttaa gctaggtcag gtcagaccag gtccaaggac agcaacttga ctgtatgcaa
     5821 cctttaccat ctttgcacag aacatacttg tagctagcta gttacactta tggaccgaaa
     5881 aggcacccca ccatgtctgt ccggctttag agtacggccg cagaccgctg atttgccttg
     5941 ccaagcagta gtcacaatgc atcgcatgag cacacgggca cgggcacggg cacaggaacc
     6001 attggcaaaa ataccagata cactataccg acgtatatca agcccaagtt taaaattcct
     6061 aaatttccgc ggctactttt caattcccta tagtgagtcg tattaaattc gtaatcatgt
     6121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6181 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6241 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6301 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7201 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7261 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7321 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7381 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7441 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7501 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7561 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7621 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7681 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7741 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7801 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7861 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7921 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7981 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8041 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8101 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8161 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8221 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
     8281 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8341 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8401 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8461 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     8521 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8581 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     8641 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8701 ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga
     8761 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     8821 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     8881 cagtgccaag ctcccgcggg gatcgactca taaaatagta accttctaat gcgtatctat
     8941 tgactaccaa ccattagtgt ggttgcagaa ggcggaattc tcccttcttc gaattcagct
     9001 tgctttttca ttttttattt tccatttttc agtttttgtt tgtgtcgaat ttagccagtt
     9061 gcttctccaa gatgaaaaaa acccctgcgc agtttctgtg ctgcaagatc ctaatcgact
     9121 tttccacccc ccacaaaagt aaatgttctt ttgttacatt cgcgtgggta gctagctccc
     9181 cgaatcttca aaggacttag ggactgcact acatcagagt gtgttcacct ggtttgctgc
     9241 ctggtttgaa agaaaagagc agggaactcg cgggttcccg gcgaataatc atgcgatagt
     9301 cctttggcct tccaagtcgc atgtagagta gacaacagac agggagggca ggaaggatct
     9361 ttcactgaga tcctgtatct tgttgggtaa gtcggatgaa aggggaatcg tatgagattg
     9421 gagaggatgc ggaagaggta acgccttttg ttaacttgtt taattattat ggggcaggcg
     9481 agagggggag gaatgtatgt gtgtgaggcg ggcgagacgg agccatccag gccaggtaga
     9541 aatagagaaa gccgaatgtt agacaatatg gcagcgtagt agagtaggta ggtaggcaag
     9601 tactgctagc aaagaggaga agggtaagct cactcttcgc attccacacc gttagtgtgt
     9661 cagtttgaac aaaaaaacaa tcatcatacc aattgatgga ctgtggactg gcttttggaa
     9721 cggcttttcg gactgcgatt attcgtgagg aatcaaggta ggaatttggt catatttacg
     9781 gacaacagtg ggtgattccc atatggagta ggaaaacgag atcatggtat cctcagatat
     9841 gttgcggaat tctgttcacc gcaaagttca gggtgctctg gtgggtttcg gttggtcttt
     9901 gctttgcttc tcccttgtct tgcatgttaa taatagccta gcctgtgagc cgaaacttag
     9961 ggtaggctta gtgttggaac gtacatatgt atcacgttga cttggtttaa ccaggcgacc
    10021 tggtagccag ccatacccac acacgttttt tgtatcttca gtatagttgt gaaaagtgta
    10081 gcggaaattt gtggtccgag caacagcgtc tttttctagt agtgcggtcg gttacttggt
    10141 tgacattggt atttggactt tgttgctaca ccattcacta cttgaagtcg agtgtgaagg
    10201 gtatgatttc tagtggtgaa cacctttagt tacgtaatgt tttcattgct gttttacttg
    10261 agatttcgat tgagaaaaag gtatttaata gctcgaatca atgtgagaac agagagaaga
    10321 tgttcttccc taactcgaaa ggtatatgag gcttgtgttt cttaggagaa ttattattct
    10381 tttgttatgt tgcgcttgta gttggaaaag gtgaagagac aaaagctgga attgtgagcg
    10441 gataacaagc tcaacacttg aaatttagga aagagcagaa tttggcaaaa aaaataaaaa
    10501 aaaaataaac acacatactc atcgag
//
