LOCUS       40924_1769       10540 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector LIC-pKLAC-LC1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Dortay H, Akula UM, Westphal C, Sittig M, Mueller-Roeber B.
  TITLE     High-Throughput Protein Expression Using a Combination of 
            Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein 
            (IFP) Detection
  JOURNAL   PLoS ONE 6 (4), E18900 (2011)
  PUBMED    21541323
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   Dortay H, Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2011) Molecular Biology, University of Potsdam, 
            Germany, Karl-Liebknecht-Str. 24-25, Haus 20, Potsdam, Brandenburg 
            14476, Germany
REFERENCE   3  (bases 1 to 10540)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10540)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "4"; pages: "E18900"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-FEB-2011) Molecular Biology, University of Potsdam, Germany, 
            Karl-Liebknecht-Str. 24-25, Haus 20, Potsdam, Brandenburg 14476, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    14..16
                     /label=start codon
                     /note="start codon"
     CDS             26..43
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             53..1036
                     /codon_start=1
                     /label=IFP1.4
                     /note="bacteriophytochrome-based monomeric infrared
                     fluorescent protein (Shu et al., 2009)"
                     /translation="MARDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELTGFDRVMLYKFAPDATGEMIAEARREGMQAFLGHRFPASHTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEELGRKLSGQVQRKEAGMDELYK"
     CDS             1037..1057
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     misc_feature    1076..1083
                     /label=PmeI recognition site
                     /note="PmeI recognition site"
     misc_feature    1084..1750
                     /label=LIC stuffer fragment
                     /note="LIC stuffer fragment"
     misc_feature    1751..1758
                     /label=PmeI recognition site
                     /note="PmeI recognition site"
     terminator      1804..2422
                     /label=LAC4 terminator
                     /note="transcription terminator for Kluyveromyces lactis
                     LAC4"
     promoter        2423..3125
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             3146..4789
                     /codon_start=1
                     /label=amdS
                     /note="acetamidase from Aspergillus nidulans"
                     /translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGIL
                     SEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALAQA
                     RELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRK
                     AGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVG
                     TDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFT
                     KSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPPILRGVE
                     TTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAIPNIKDLL
                     NPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPTAAVRHDQF
                     RYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYDPEAYHGAPV
                     AVQVIGRRLSEERTLAIAEEVGKLLGNVVTP"
     promoter        5428..6084
                     /label=LAC4 promoter (5')
                     /note="5' portion of the Kluyveromyces lactis LAC4
                     promoter"
     promoter        complement(6101..6119)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6133..6149)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6157..6173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6181..6211)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6226..6247)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6535..7123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7297..8154)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8155..8259)
                     /label=AmpR promoter
     rep_origin      8286..8741
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8882..8898
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8912..10440
                     /label=LAC4 promoter (3')
                     /note="3' portion of a modified Kluyveromyces lactis LAC4 
                     promoter"
     protein_bind    10446..10462
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aagcttgaaa aaaatgggct ccggccatca ccatcaccat cacctcgaat caatggctcg
       61 ggaccctctg ccattctttc cacctctgta cctgggcggc cctgagatta caaccgagaa
      121 ctgcgagaga gagcctatcc acattcctgg gtccatccag ccacacgggg ctctgctcac
      181 agctgacggc cactccggag aggtgctcca agtgtccctg aatgccgcta ccttcctggg
      241 ccaggagcct actgtgctgc gggggcagac cctggctgcc ctgctccccg agcagtggcc
      301 agccctgcag gcagccctgc ccccaggatg tccagatgcc ctccaataca gggccaccct
      361 cgactggcca gctgctgggc acctcagcct gactgtgcat cgggtggctg aactcctgat
      421 cctggagttc gaacctaccg aggcctggga cagcattggc cctcacgccc tgaggaacgc
      481 catgtttgct ctggaaagcg ctccaaacct gcgggctctg gccgaagtcg caacacaaac
      541 tgtgagagaa ctgactggct tcgatcgggt gatgctgtac aaatttgccc ctgacgccac
      601 tggagagatg attgctgagg ccagacggga gggcatgcag gcttttctgg gccacaggtt
      661 tcccgcatcc cacacccctg cacaagctag ggccctctac acaagacacc tgctccggct
      721 gaccgcagac accagggctg cagcagtgcc cctcgacccc gtgctgaatc cccagacaaa
      781 tgctcctaca cctctgggcg gagctgtcct cagagctaca tccccaatgc acatgcagta
      841 cctgaggaat atgggagtgg gctcctccct gagcgtcagc gtcgtggtcg gcggccagct
      901 gtggggactg attgtctgcc accatcagac accctacgtg ctgccaccag atctgcggac
      961 caccctggag gagctgggga gaaagctgtc cgggcaggtg cagaggaaag aagccggaat
     1021 ggacgagctg tataaagaga acctgtattt tcagggcggc ggcatgggtt cttctgttta
     1081 aacctcagtg tcatcattcg aaagattcga aatatttccc aaagaaagct catggcccaa
     1141 ccacaacaca aacaaaggag taacggcagc atgctcccat gcgggggaaa gcagttttta
     1201 cagaaatttg ctatggctga cggagaagga gggctcatac ccaaagctga aaaattctta
     1261 tgtgaacaag agagggaaag aagtccttgt actgtggggt attcatcacc cgtctaacag
     1321 taaggagcaa cagaatatct atcagaatga aaatgcttat gtctctgtag tgacttcaaa
     1381 ttataacagg agatttaccc cggaaatagc agaaagaccc aaagtaagag atcaagctgg
     1441 gaggatgaac tattactgga ccttgctaaa acccggagac acaataatat ttgaggcaaa
     1501 tggaaatcta atagcaccaa ggtatgcttt cgcactgagt agaggctttg ggtccggcat
     1561 catcacctca aacgcatcaa tgcatgagtg taacacgaag tgtcaaacac ccctgggagc
     1621 tataaacagc agtctccctt tccagaatat acacccagtc acaataggag agtgcccaaa
     1681 atacgtcagg agtgccaaat tgaggatggt tacaggacta aggaacattc cgtccattca
     1741 atccgctagc gtttaaacag ggcgagaacc tgcggccgct taattaaggc cttgaatcga
     1801 gaatttatac ttagataagt atgtacttac aggtatattt ctatgagata ctgatgtata
     1861 catgcatgat aatatttaaa cggttattag tgccgattgt cttgtgcgat aatgacgttc
     1921 ctatcaaagc aatacactta ccacctatta catgggccaa gaaaatattt tcgaacttgt
     1981 ttagaatatt agcacagagt atatgatgat atccgttaga ttatgcatga ttcattccta
     2041 caactttttc gtagcataag gattaattac ttggatgcca ataaaaaaaa aaaacatcga
     2101 gaaaatttca gcatgctcag aaacaattgc agtgtatcaa agtaaaaaaa agattttcac
     2161 tacatgttcc ttttgaagaa agaaaatcat ggaacattag atttacaaaa atttaaccac
     2221 cgctgattaa cgattagacc gttaagcgca caacaggtta ttagtacaga gaaagcattc
     2281 tgtggtgttg ccccggactt tcttttgcga cataggtaaa tcgaatacca tcatactatc
     2341 ttttccaatg actccctaaa gaaagactct tcttcgatgt tgtatacgtt ggagcatagg
     2401 gcaagaattg tggcttgaga tcatcctttt gttgtttccg ggtgtacaat atggacttcc
     2461 tcttttctgg caaccaaacc catacatcgg gattcctata ataccttcgt tggtctccct
     2521 aacatgtagg tggcggaggg gagatataca atagaacaga taccagacaa gacataatgg
     2581 gctaaacaag actacaccaa ttacactgcc tcattgatgg tggtacataa cgaactaata
     2641 ctgtagccct agacttgata gccatcatca tatcgaagtt tcactaccct ttttccattt
     2701 gccatctatt gaagtaataa taggcgcatg caacttcttt tctttttttt tcttttctct
     2761 ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa gacactaaag
     2821 gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg
     2881 tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag
     2941 caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc
     3001 ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct
     3061 cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac caagcataca
     3121 atcaaggaat tccggatccg ccaccatgcc tcaatcctgg gaagaactgg ccgctgataa
     3181 gcgcgcccgc ctcgcaaaaa ccatccctga tgaatggaaa gtccagacgc tgcctgcgga
     3241 agacagcgtt attgatttcc caaagaaatc ggggatcctt tcagaggccg aactgaagat
     3301 cacagaggct tccgctgcgg atcttgtgtc caagctggcg gccggagagt tgacctcggt
     3361 ggaagttacg ctagcattct gtaaacgggc agcaatcgcc cagcagttaa caaactgcgc
     3421 ccacgagttc ttccctgacg ccgctctcgc gcaggcaagg gaactcgatg aatactacgc
     3481 aaagcacaag agacccgttg gtccactcca tggcctcccc atctctctca aagaccagct
     3541 tcgagtcaag ggctacgaaa catcaatggg ctacatctca tggctaaaca agtacgacga
     3601 aggggactcg gttctgacaa ccatgctccg caaagccggt gccgtcttct acgtcaagac
     3661 ctctgtcccg cagaccctga tggtctgcga gacagtcaac aacatcatcg ggcgcaccgt
     3721 caacccacgc aacaagaact ggtcgtgcgg cggcagttct ggtggtgagg gtgcgatcgt
     3781 tgggattcgt ggtggcgtca tcggtgtagg aacggatatc ggtggctcga ttcgagtgcc
     3841 ggccgcgttc aacttcctgt acggtctaag gccgagtcat gggcggctgc cgtatgcaaa
     3901 gatggcgaac agcatggagg gtcaggagac ggtgcacagc gttgtcgggc cgattacgca
     3961 ctctgttgag gacctccgcc tcttcaccaa atccgtcctc ggtcaggagc catggaaata
     4021 cgactccaag gtcatcccca tgccctggcg ccagtccgag tcggacatta ttgcctccaa
     4081 gatcaagaac ggcgggctca atatcggcta ctacaacttc gacggcaatg tccttccaca
     4141 ccctcctatc ctgcgcggcg tggaaactac cgtcgccgca ctcgccaaag ccggtcacac
     4201 cgtgaccccg tggacgccat acaagcacga tttcggccac gatctcatct cccatatcta
     4261 cgcggctgac ggcagcgccg acgtaatgcg cgatatcagt gcatccggcg agccggcgat
     4321 tccaaatatc aaagacctac tgaacccgaa catcaaagct gttaacatga acgagctctg
     4381 ggacacgcat ctccagaagt ggaattacca gatggagtac cttgagaaat ggcgggaggc
     4441 tgaagaaaag gccgggaagg aactggacgc catcatcgcg ccgattacgc ctaccgctgc
     4501 ggtacggcat gaccagttcc ggtactatgg gtatgcctct gtgatcaacc tgctggattt
     4561 cacgagcgtg gttgttccgg ttacctttgc ggataagaac atcgataaga agaatgagag
     4621 tttcaaggcg gttagtgagc ttgatgccct cgtgcaggaa gagtatgatc cggaggcgta
     4681 ccatggggca ccggttgcag tgcaggttat cggacggaga ctcagtgaag agaggacgtt
     4741 ggcgattgca gaggaagtgg ggaagttgct gggaaatgtg gtgactccat agcccggggg
     4801 gggctcgatc ccctcgcgag ttggttcagc tgctgcctga ggctggacga cctcgcggag
     4861 ttctaccggc agtgcaaatc cgtcggcatc caggaaacca gcagcggcta tccgcgcatc
     4921 catgcccccg aactgcagga gtggggaggc acgatggccg ctttggtcga tctagattac
     4981 gtggaagaaa ggtagtaaaa gtagtagtat aagtagtaaa aagaggtaaa aagagaaaac
     5041 cggctacata ctagagaagc acgtacacaa aaactcatag gcacttcatc atacgacagt
     5101 ttcttgatgc attataatag tgtattagat attttcagaa atatgcatag aacctcctct
     5161 tgcctttact ttttatacat agaacattgg cagatttact tacactactt tgtttctacg
     5221 ccatttcttt tgttttcaac acttagacaa gttgttgaga accggactac taaaaagcaa
     5281 tgttcccact gaaaatcatg tacctgcagg ataataaccc cctaattctg catcgatcca
     5341 gtatgttttt ttttctctac tcatttttac ctgaagatag agcttctaaa acaaaaaaaa
     5401 tcagtgatta catgcatatt gtgtgttcta gtaaccaaag gaaaggaaca gatagataaa
     5461 attccgagac tgtcaaatta ggtttttttc tttttttttg gcgggagtca gtgggccgaa
     5521 atatgttctt ggcctagaac ttaatctggt ttgatcatgc caatacttgc ctgagtgccc
     5581 gactttttgc ccaccctctt gccttctgtc atccttcaaa acccacctgt tttccagccg
     5641 tatcttcgct cgcatctaca catactgtgc catatcttgt gtgtagccgg acgtgactat
     5701 gaccaaaaac aaacaaggag aactgttcgc cgatttgtaa cactcctgca tccatccaag
     5761 tgggtatgcg ctatgcaatg ttaagctagg tcaggtcaga ccaggtccaa ggacagcaac
     5821 ttgactgtat gcaaccttta ccatctttgc acagaacata cttgtagcta gctagttaca
     5881 cttatggacc gaaaaggcac cccaccatgt ctgtccggct ttagagtacg gccgcagacc
     5941 gctgatttgc cttgccaagc agtagtcaca atgcatcgca tgagcacacg ggcacgggca
     6001 cgggcacagg aaccattggc aaaaatacca gatacactat accgacgtat atcaagccca
     6061 agtttaaaat tcctaaattt ccgcggctac ttttcaattc cctatagtga gtcgtattaa
     6121 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6181 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6241 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6301 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6361 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6421 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6481 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6541 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6601 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6661 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6721 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6781 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6841 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6901 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6961 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7021 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7081 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7141 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7201 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7261 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7321 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7381 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7441 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7501 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7561 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     7621 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7681 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7741 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7801 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7861 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7921 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7981 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8041 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8101 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8161 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8221 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8281 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8341 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8401 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8461 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8521 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8581 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8641 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8701 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
     8761 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8821 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8881 tgtaaaacga cggccagtgc caagctcccg cggggatcga ctcataaaat agtaaccttc
     8941 taatgcgtat ctattgacta ccaaccatta gtgtggttgc agaaggcgga attctccctt
     9001 cttcgaattc agcttgcttt ttcatttttt attttccatt tttcagtttt tgtttgtgtc
     9061 gaatttagcc agttgcttct ccaagatgaa aaaaacccct gcgcagtttc tgtgctgcaa
     9121 gatcctaatc gacttttcca ccccccacaa aagtaaatgt tcttttgtta cattcgcgtg
     9181 ggtagctagc tccccgaatc ttcaaaggac ttagggactg cactacatca gagtgtgttc
     9241 acctggtttg ctgcctggtt tgaaagaaaa gagcagggaa ctcgcgggtt cccggcgaat
     9301 aatcatgcga tagtcctttg gccttccaag tcgcatgtag agtagacaac agacagggag
     9361 ggcaggaagg atctttcact gagatcctgt atcttgttgg gtaagtcgga tgaaagggga
     9421 atcgtatgag attggagagg atgcggaaga ggtaacgcct tttgttaact tgtttaatta
     9481 ttatggggca ggcgagaggg ggaggaatgt atgtgtgtga ggcgggcgag acggagccat
     9541 ccaggccagg tagaaataga gaaagccgaa tgttagacaa tatggcagcg tagtagagta
     9601 ggtaggtagg caagtactgc tagcaaagag gagaagggta agctcactct tcgcattcca
     9661 caccgttagt gtgtcagttt gaacaaaaaa acaatcatca taccaattga tggactgtgg
     9721 actggctttt ggaacggctt ttcggactgc gattattcgt gaggaatcaa ggtaggaatt
     9781 tggtcatatt tacggacaac agtgggtgat tcccatatgg agtaggaaaa cgagatcatg
     9841 gtatcctcag atatgttgcg gaattctgtt caccgcaaag ttcagggtgc tctggtgggt
     9901 ttcggttggt ctttgctttg cttctccctt gtcttgcatg ttaataatag cctagcctgt
     9961 gagccgaaac ttagggtagg cttagtgttg gaacgtacat atgtatcacg ttgacttggt
    10021 ttaaccaggc gacctggtag ccagccatac ccacacacgt tttttgtatc ttcagtatag
    10081 ttgtgaaaag tgtagcggaa atttgtggtc cgagcaacag cgtctttttc tagtagtgcg
    10141 gtcggttact tggttgacat tggtatttgg actttgttgc tacaccattc actacttgaa
    10201 gtcgagtgtg aagggtatga tttctagtgg tgaacacctt tagttacgta atgttttcat
    10261 tgctgtttta cttgagattt cgattgagaa aaaggtattt aatagctcga atcaatgtga
    10321 gaacagagag aagatgttct tccctaactc gaaaggtata tgaggcttgt gtttcttagg
    10381 agaattatta ttcttttgtt atgttgcgct tgtagttgga aaaggtgaag agacaaaagc
    10441 tggaattgtg agcggataac aagctcaaca cttgaaattt aggaaagagc agaatttggc
    10501 aaaaaaaata aaaaaaaaat aaacacacat actcatcgag
//
