LOCUS       40924_1579        9050 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector L401-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich BV, Samoilovich MP, Satou Y, Kruss M, Gelhaus C, Kurn U, 
            Bosch TC, Khalturin K.
  TITLE     Blood System Formation in the Urochordate Ciona intestinalis 
            Requires the Variable Receptor vCRL1
  JOURNAL   Mol. Biol. Evol. 29 (10), 3081-3093 (2012)
  PUBMED    22513285
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich VB, Samoilovich MP, Satou Y, Kruess M, Gelhaus C, Kuern U,
            Bosch TCG., Khalturin K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2011) Zoological Institute, Christian-Albrechts 
            University Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 
            24118, Germany
REFERENCE   3  (bases 1 to 9050)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9050)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. 
            Evol."; date: "2012"; volume: "29"; issue: "10"; pages: "3081-3093"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2011) Zoological Institute, Christian-Albrechts University 
            Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 24118, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2531..3244
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(6207..6225)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6235..6251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6393..6848
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6874..6978
                     /label=AmpR promoter
     CDS             6979..7836
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8010..8598
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8886..8907
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8922..8952
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8960..8976
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8984..9000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9021..9039
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccaagt gcttgaaatg ctaaatgttt tcaatttttc gccattaaga caagcctaca
       61 caaatgcttc tataaattat gccaagcacg ttagcagctt ctacgagccc caaccactat
      121 taattcgaac agcatgtttt tttgcagtgc gcaatgttta acacactata ttatcaatac
      181 tactaaagat aacacatacc aatgcatttc gtctcaaaga gaattttatt ctcttcacga
      241 cgaaaaaaaa agttttgctc tatttccaac aacaacaaaa atatgagtaa tttattcaaa
      301 cggtttgctt aagagataag aaaaaagtga ccactattaa ttcgaacgcg gcgtaagctt
      361 accttaatct caagaagagc aaaacaaaag caactaatgt aacggaatca ttatctagtt
      421 atgatctgca aataatgctg caggctttgg ttataggcta ttgtttgagg atccgagggg
      481 caactgttta ttaattttga tatttaacat tttatatgcc atgtgtgttt ctgcaagcca
      541 tcttgaagta taatcgagca cggtattagt tttgcgtata aagatataac cagcggggct
      601 gatcgcatat atatagctat agcttacgca cgaataatat ggcgatgtct gactgacgat
      661 cattaaaagc atgtatacaa ccaactacag gttatgggaa atatagtagg gtgggggaag
      721 atgtgacacc tttagcacat aatatccaaa tatcatgata gtgttttaaa gaattaacaa
      781 cggtcaatgg aagtcgtgag gatacggttt aataattcta tgaatgtttt tggttactaa
      841 caaatgagac gataaaatag aatgtaatag tgtctcatct ttccccaacc cactataaat
      901 acagcaaatt gtaagttatg ggaaaaaata caacagtgaa agtataacag tttttgtttc
      961 taacgctata atagcaacag cagaataaac aggtagtacg tatgcatgca ctgattcaag
     1021 actgaaatca aaagaaaaca ttgttgttgt tttggttcga tacttaactc atgttgttta
     1081 agtttttaat cgtgttttga ggaagttccg ctgctttgcg cgagcggcgg aactctggtt
     1141 ccgagaatgt ttgatgtcac gtttccttta gcgaatcttt aaagaaatat caaagttttt
     1201 gtttaacctc ttattcgcga ggacacaatt atgattcgtt taatgagtct gttagttgtg
     1261 cgggacatta ctgggtaaaa ttaatagaaa aatgccaaaa aaagctgaac gattaaattt
     1321 tgagctttct tagaaaaatg agtaaaaatg gcaaatgtag gaagtgctat tatgacgcat
     1381 gttgtagatg atcgaaagcg agtaatgcgt aatatgtctg gcacagctgc aatgttaata
     1441 catcacaatt taattactat acaagagcaa tagttcattt aatatgccac aaagtacgtt
     1501 gccggtgcgc agtgaaaaat atcacagaca atacacgttc ttccgttgac ggacttgaaa
     1561 tacgtgtagc tgataagtga aggaacctga gttgacaaac gaattcttcc ggcaaacaga
     1621 gaccgcattt tcacgatttt ggtttaccgt agcataaata cgtaatattt tgttttggtt
     1681 ttatacaccg acagccggtg tattttgcaa ggattacctg cttcctattt gacaacaaac
     1741 attgcaaatg atggagaata aacaaataat aaatccgacc gaagcaaaaa tattttgcaa
     1801 gcaatctctt ttatatgtgg gtgaggtgcc attgggtggc acgccatggg ttctaggatt
     1861 taagccagaa ttagtggcta taggtacatt aataactgtt ctgacatttc attgtattta
     1921 atgtaatgaa ctatacaaaa ggcacatata tttaaatccg ccaccttaca aaagtaaaaa
     1981 atccgatttc tatattagca ttaatggcta tgaaatagtt tcagttctta acgtcaggta
     2041 attccaaagt attttatttg atttaattag aggatttatt ccttgcctca cagtgaaact
     2101 tattaagcag cttaaacttt tgcggttcgt gcacgaattt cgtggtatga cgtcattata
     2161 tcacaatgta gtggaaaagt taacgttaca ttaaaaaaaa aacgattaat ttgtaaaacg
     2221 taataaggtt atgcagcttg agtttgacga aagcagggct cagttatacg tcatgatacg
     2281 aacgtatatg tctacgaaca ttggcgcgcc ggaattcgct aagtagcaat gtgtatgagt
     2341 aattaggcat cattactcag agccatatta ggggcatcga tagtgcgcag tgaaaatgac
     2401 ggaaaaacgc aacggcgggt atataaggcc tgcgtggatg aggtatatat gacacgttca
     2461 tcgaacgaag ttaaaatatt attttgtgaa gttattaaat atgaagctgc tactattaat
     2521 taatgaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     2581 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     2641 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     2701 gccaacactt gtcactactt tctgttatgg tgttcaatgc ttttcaagat acccagatca
     2761 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     2821 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     2881 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     2941 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     3001 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     3061 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     3121 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     3181 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     3241 caaagcgatc gcgttttaaa attctgggca tttcacactt tttatacaat attggtatca
     3301 ctttgttctt attaaattca aatgataaag ataatgcaat tttagcttgt gttcgacagt
     3361 tatttgattt gttgaaatta ggagataaaa taactataac tttcctgcat ttttcttcaa
     3421 taacactgca taactgctca aagggattgt acccaggcaa aaaatcacaa taatcaatac
     3481 aaactttata cccatattca ttctctaaac gattcttaat taacttagca aaatttatat
     3541 catcttttgc atatgatata aagacgtcat aattatcatt gagtgtaaaa ttactaacat
     3601 gagttatgga gcattgcaaa tcttgattgt cattttcaat aaaattgtta tcattaatcg
     3661 tacgaaacca atggattgca ttaataaaga tcaattactt tatcagtctc tgtcatatcc
     3721 tataatgagt caagtttcat ttttacttaa tcctatttgt gataataaag attggagagc
     3781 cttagctggt gtatttgatt ttacgatgga agaagtgaat ggtttgttat ctgcttgtga
     3841 cccaacaatg gagttattta aatattatga gttgaaatat ggcaaaaaag ttacattatc
     3901 atatgttata gaatcattaa ataatattgg aagaaaagat gttgtacaag atattcttaa
     3961 atacattaat gataacaatt ttattgaaaa tgacaatcaa gatttgcaat gctccataac
     4021 tcatgttagt aattttacac tcaatgataa ttatgacgtc tttatatcat atgcaaaaga
     4081 tgatataaat tttgctaagt taattaagaa tcgtttagag aatgaatatg ggtataaagt
     4141 ttgtattgat tattgtgatt ttttgcctgg gtacaatccc tttgagcagt tatgcagtgt
     4201 tattgaagaa aaatgcagga aagttatagt tattttatct cctaatttca acaaatcaaa
     4261 taactgtcga acacaagcta aaattgcatt atctttatca tttgaattta ataagaacaa
     4321 agtgatacca atattgtata aaaagtgtga aatgcccaga attttaaaac gaattctaca
     4381 tcgcgtgact gcagccaacg acaccgccca ccatcacttc tgcagtttta ctccaccagc
     4441 ttgctcaccc ttctaaccat ttcagcctag ttaaaccccc agttcaattt gtcagccttt
     4501 taaattgcgt catgacattt cagttgttac tgtgtgccgc tctgtccaca tggtgtagtt
     4561 atgtttttca ttacccccac cgaaaacact ttcgtaggaa aaaaccctcc gtttcgtgcc
     4621 aacttaaaag tgttattttt gtttctagcg ctgcttaact tatttaaaat gacaagacta
     4681 tctatgattt taaaccatgg ctttgaatgg cgtacatgta ttattgtgtt tatattcagt
     4741 taaaccgtac tattactgct tggaaaattc agttcattcc ttcaccgaag gctttaacgt
     4801 ggttggttgc ggtgttgtca atttgctgct ggaaataaag cttctgttaa gtgtatactg
     4861 tctcttggtt tgcaagatta ttatggatcc agatgttgta tatacaagag tgaagattta
     4921 gcgtttttat gtggtaaaac gcccatggtg atctatttac agtttttaag ttgagctgat
     4981 caattttcac atgatgatcc tgaacgacca ggggcacaaa ggcatttgca gattgtccca
     5041 tctctcacta cgaccgatct accactattt tcacaacggc cagtacattt cgaagtctgg
     5101 tatttagcga agtagtgttc aattgccaaa gccatgtttc tctgcttcgc tctgtctggg
     5161 atgaccgatg atatgggtcg cagctgtaaa attacaaata gtcagttttt ctgacgaaag
     5221 tgaatgttga ttacattcaa atgaaagtcg ttgatggcta atgccatcgt tcatacgctt
     5281 taattagcag attatgtggt tatattagat atctttacca gtaatattga gaacgccaag
     5341 gtaaaatcta gttttttgat acttaatgaa cagccatatt aaaagccata catgaaaaaa
     5401 aaattaaaaa aaacatacat aaactctcat aagctgacac agtaggtcgt ggctgacacg
     5461 aggtgtatga aacaaaacgc tcgtgtttta acgactgttg ttgcaccgtc acgcgagaat
     5521 aaagtaactt ccattcatac atgatacagt tcttaccttg aaatcgatga ctgcagggct
     5581 aagtttaact gatgctgtcc agttttcgta agcatttgtt gtaagtcggt ttctagcaaa
     5641 gaaactgaga gcactagctg tatgagactg cccaccaatc acattcgata taacttcctt
     5701 agcagccatt gtaaaacttc ctaaatatta gtaaataacg aataataatg tgtacatgac
     5761 tctgcttatc agttagattg ggcgcgccat cagtttaaac gaatgcggcc gccaccgcgg
     5821 gacgttacac aattctaata ttaattaaat tattgtttta agtatgatag taaatcacat
     5881 tacgccgcgt tcgaattaat agtggtcact tttttcttat ctcttaagca aaccgtttga
     5941 ataaattact catatttttg ttgttgttgg aaatagagca aaactttttt tttcgtcgtg
     6001 aagagaataa aattctcttt gagacgaaat gcattggtat gtgttatctt tagtagtatt
     6061 gataatatag tgtgttaaac attgcgcact gcaaaaaaaa catgctgttc gaattaatag
     6121 tggttggggc tcgtagaaaa cgaaaaatat cttaagctag catagagaat ggagcaaaac
     6181 tcaatttgat gcgagctcca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     6241 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6301 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6361 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6421 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6481 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6541 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6601 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6661 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6721 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6781 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     6841 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6901 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6961 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7021 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7081 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7141 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7201 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7261 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7321 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7381 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7441 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7501 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7561 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7621 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7681 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7741 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7801 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     8161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8701 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8761 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8821 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8881 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8941 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9001 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
//
