LOCUS       40924_1569        9752 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector L360-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich BV, Samoilovich MP, Satou Y, Kruss M, Gelhaus C, Kurn U, 
            Bosch TC, Khalturin K.
  TITLE     Blood System Formation in the Urochordate Ciona intestinalis 
            Requires the Variable Receptor vCRL1
  JOURNAL   Mol. Biol. Evol. 29 (10), 3081-3093 (2012)
  PUBMED    22513285
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich VB, Samoilovich MP, Satou Y, Kruess M, Gelhaus C, Kuern U,
            Bosch TCG., Khalturin K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2011) Zoological Institute, Christian-Albrechts 
            University Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 
            24118, Germany
REFERENCE   3  (bases 1 to 9752)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9752)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. 
            Evol."; date: "2012"; volume: "29"; issue: "10"; pages: "3081-3093"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2011) Zoological Institute, Christian-Albrechts University 
            Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 24118, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2531..3244
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     regulatory      4202..4211
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4242..4260)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4267..4283)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6909..6927)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6937..6953)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7095..7550
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7576..7680
                     /label=AmpR promoter
     CDS             7681..8538
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8712..9300
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9588..9609
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9624..9654
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9662..9678
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9686..9702
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9723..9741
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccaagt gcttgaaatg ctaaatgttt tcaatttttc gccattaaga caagcctaca
       61 caaatgcttc tataaattat gccaagcacg ttagcagctt ctacgagccc caaccactat
      121 taattcgaac agcatgtttt tttgcagtgc gcaatgttta acacactata ttatcaatac
      181 tactaaagat aacacatacc aatgcatttc gtctcaaaga gaattttatt ctcttcacga
      241 cgaaaaaaaa agttttgctc tatttccaac aacaacaaaa atatgagtaa tttattcaaa
      301 cggtttgctt aagagataag aaaaaagtga ccactattaa ttcgaacgcg gcgtaagctt
      361 accttaatct caagaagagc aaaacaaaag caactaatgt aacggaatca ttatctagtt
      421 atgatctgca aataatgctg caggctttgg ttataggcta ttgtttgagg atccgagggg
      481 caactgttta ttaattttga tatttaacat tttatatgcc atgtgtgttt ctgcaagcca
      541 tcttgaagta taatcgagca cggtattagt tttgcgtata aagatataac cagcggggct
      601 gatcgcatat atatagctat agcttacgca cgaataatat ggcgatgtct gactgacgat
      661 cattaaaagc atgtatacaa ccaactacag gttatgggaa atatagtagg gtgggggaag
      721 atgtgacacc tttagcacat aatatccaaa tatcatgata gtgttttaaa gaattaacaa
      781 cggtcaatgg aagtcgtgag gatacggttt aataattcta tgaatgtttt tggttactaa
      841 caaatgagac gataaaatag aatgtaatag tgtctcatct ttccccaacc cactataaat
      901 acagcaaatt gtaagttatg ggaaaaaata caacagtgaa agtataacag tttttgtttc
      961 taacgctata atagcaacag cagaataaac aggtagtacg tatgcatgca ctgattcaag
     1021 actgaaatca aaagaaaaca ttgttgttgt tttggttcga tacttaactc atgttgttta
     1081 agtttttaat cgtgttttga ggaagttccg ctgctttgcg cgagcggcgg aactctggtt
     1141 ccgagaatgt ttgatgtcac gtttccttta gcgaatcttt aaagaaatat caaagttttt
     1201 gtttaacctc ttattcgcga ggacacaatt atgattcgtt taatgagtct gttagttgtg
     1261 cgggacatta ctgggtaaaa ttaatagaaa aatgccaaaa aaagctgaac gattaaattt
     1321 tgagctttct tagaaaaatg agtaaaaatg gcaaatgtag gaagtgctat tatgacgcat
     1381 gttgtagatg atcgaaagcg agtaatgcgt aatatgtctg gcacagctgc aatgttaata
     1441 catcacaatt taattactat acaagagcaa tagttcattt aatatgccac aaagtacgtt
     1501 gccggtgcgc agtgaaaaat atcacagaca atacacgttc ttccgttgac ggacttgaaa
     1561 tacgtgtagc tgataagtga aggaacctga gttgacaaac gaattcttcc ggcaaacaga
     1621 gaccgcattt tcacgatttt ggtttaccgt agcataaata cgtaatattt tgttttggtt
     1681 ttatacaccg acagccggtg tattttgcaa ggattacctg cttcctattt gacaacaaac
     1741 attgcaaatg atggagaata aacaaataat aaatccgacc gaagcaaaaa tattttgcaa
     1801 gcaatctctt ttatatgtgg gtgaggtgcc attgggtggc acgccatggg ttctaggatt
     1861 taagccagaa ttagtggcta taggtacatt aataactgtt ctgacatttc attgtattta
     1921 atgtaatgaa ctatacaaaa ggcacatata tttaaatccg ccaccttaca aaagtaaaaa
     1981 atccgatttc tatattagca ttaatggcta tgaaatagtt tcagttctta acgtcaggta
     2041 attccaaagt attttatttg atttaattag aggatttatt ccttgcctca cagtgaaact
     2101 tattaagcag cttaaacttt tgcggttcgt gcacgaattt cgtggtatga cgtcattata
     2161 tcacaatgta gtggaaaagt taacgttaca ttaaaaaaaa aacgattaat ttgtaaaacg
     2221 taataaggtt atgcagcttg agtttgacga aagcagggct cagttatacg tcatgatacg
     2281 aacgtatatg tctacgaaca ttggcgcgcc ggaattcgct aagtagcaat gtgtatgagt
     2341 aattaggcat cattactcag agccatatta ggggcatcga tagtgcgcag tgaaaatgac
     2401 ggaaaaacgc aacggcgggt atataaggcc tgcgtggatg aggtatatat gacacgttca
     2461 tcgaacgaag ttaaaatatt attttgtgaa gttattaaat atgaagctgc tactattaat
     2521 taatgaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     2581 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     2641 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     2701 gccaacactt gtcactactt tctgttatgg tgttcaatgc ttttcaagat acccagatca
     2761 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     2821 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     2881 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     2941 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     3001 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     3061 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     3121 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     3181 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     3241 caaagcgatc gcttaccggt catttataaa gataggcgtg aaaggtacag taaaaatcaa
     3301 tatatttatg tggggggttt atattaattt aaatatataa catgctttac tattggtggt
     3361 gtttctagta caataaaatc atgcatacat ctacgtggaa caaatacaaa attagtaata
     3421 atacaattac gttgtttaat aaaccatatg tacataagaa gaaactgagt tacacagaat
     3481 attgaatacg tttctcaaac tttaattaaa ataaaatggt tgtcttgaag actcaattta
     3541 tacaaacaat tttaaacacg aacaaacaga taaaattaaa acctgggaca tgcaaagaat
     3601 attcaaaaca aaattatgaa ccaaagaaac tgctaagaat ttgaaagtaa acaaatttct
     3661 tgtataatgt tcaaggtaca acaccttcgc cattaggacc gtccggctgt gtattctttt
     3721 tcaagtctgg aatggttata tctggctctg gatcttttgt tccccaagac aagcaaagta
     3781 ttgaaaaagc acaacagcaa atctccgtac aaaagatagg aatcaggaag aatatgatga
     3841 gtgcaacaac aattatgcat ccaataacaa tgcccgctat agctccatca gatagtgatt
     3901 tacttctcga actttgaata cagacagggt cactgtcaaa tgtagcatcc tcctggcatg
     3961 ttgcattaac atcgttccca gtatgatcag ttgcacatga atattgaact ttaattatca
     4021 tggacaatgt ggtaacaaca gctaaatcac aatttacggt aactgtgctg ttgcttgtca
     4081 atgaagtaag atatgtaaga gtggtcataa tgactcctgt tagtcctgtt ccttcccacg
     4141 aacatgcagt tgggctagtt gatactgttg cagtgatcat caggtttgtg gaatcccgcg
     4201 gccatggcgg ccgggagcat gcgacgtcgg gcccaattcg ccctatagtg agtcgtatta
     4261 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccgtac
     4321 ggttcaatat tcatgtgcaa ctgatcatac tgggaacgat gttaatgcaa catgccagga
     4381 ggatgctaca tttgacagtg accctgtctg tattcaaagt tcgagaagta aatcactatc
     4441 tgatggagct atagcgggca ttgttattgg atgcataatt gttgttgcac tcatcatatt
     4501 cttcctgatt cctatctttt gtacggagat ttgctgttgt gctttttcaa tactttgctt
     4561 gtcttgggga acaaaagatc cagagccaga tataaccatt ccagacttga aaaagaatac
     4621 acagccggac ggtcctaatg gcgaaggtgt tgtaccttga acattataca agaaatttgt
     4681 ttactttcaa attcttagca gtttctttgg ttcataattt tgttttgaat attctttgca
     4741 tgttccaggt tttaatttta tctgtttgtt cgtgtttaaa attgtttgta taaattgagt
     4801 cttcaagaca accattttat tttaattaaa gcttgagaaa cgtattcaat attctgtgta
     4861 actcagtttc ttcttatgta catatggttt attaaacaac gtaattgtat tattactaat
     4921 tttgtatttg ttccacgtag atgtatgcat gattttattg tactagaaac accaccaata
     4981 gtaaagcatg ttatatattt aaattaatat aaacccccca cataaatata ttgattttta
     5041 ctgtaccttt cacgcctatc tttataaatg gaattcttgc gatcgcgtga ctgcagccaa
     5101 cgacaccgcc caccatcact tctgcagttt tactccacca gcttgctcac ccttctaacc
     5161 atttcagcct agttaaaccc ccagttcaat ttgtcagcct tttaaattgc gtcatgacat
     5221 ttcagttgtt actgtgtgcc gctctgtcca catggtgtag ttatgttttt cattaccccc
     5281 accgaaaaca ctttcgtagg aaaaaaccct ccgtttcgtg ccaacttaaa agtgttattt
     5341 ttgtttctag cgctgcttaa cttatttaaa atgacaagac tatctatgat tttaaaccat
     5401 ggctttgaat ggcgtacatg tattattgtg tttatattca gttaaaccgt actattactg
     5461 cttggaaaat tcagttcatt ccttcaccga aggctttaac gtggttggtt gcggtgttgt
     5521 caatttgctg ctggaaataa agcttctgtt aagtgtatac tgtctcttgg tttgcaagat
     5581 tattatggat ccagatgttg tatatacaag agtgaagatt tagcgttttt atgtggtaaa
     5641 acgcccatgg tgatctattt acagttttta agttgagctg atcaattttc acatgatgat
     5701 cctgaacgac caggggcaca aaggcatttg cagattgtcc catctctcac tacgaccgat
     5761 ctaccactat tttcacaacg gccagtacat ttcgaagtct ggtatttagc gaagtagtgt
     5821 tcaattgcca aagccatgtt tctctgcttc gctctgtctg ggatgaccga tgatatgggt
     5881 cgcagctgta aaattacaaa tagtcagttt ttctgacgaa agtgaatgtt gattacattc
     5941 aaatgaaagt cgttgatggc taatgccatc gttcatacgc tttaattagc agattatgtg
     6001 gttatattag atatctttac cagtaatatt gagaacgcca aggtaaaatc tagttttttg
     6061 atacttaatg aacagccata ttaaaagcca tacatgaaaa aaaaattaaa aaaaacatac
     6121 ataaactctc ataagctgac acagtaggtc gtggctgaca cgaggtgtat gaaacaaaac
     6181 gctcgtgttt taacgactgt tgttgcaccg tcacgcgaga ataaagtaac ttccattcat
     6241 acatgataca gttcttacct tgaaatcgat gactgcaggg ctaagtttaa ctgatgctgt
     6301 ccagttttcg taagcatttg ttgtaagtcg gtttctagca aagaaactga gagcactagc
     6361 tgtatgagac tgcccaccaa tcacattcga tataacttcc ttagcagcca ttgtaaaact
     6421 tcctaaatat tagtaaataa cgaataataa tgtgtacatg actctgctta tcagttagat
     6481 tgggcgcgcc atcagtttaa acgaatgcgg ccgccaccgc gggacgttac acaattctaa
     6541 tattaattaa attattgttt taagtatgat agtaaatcac attacgccgc gttcgaatta
     6601 atagtggtca cttttttctt atctcttaag caaaccgttt gaataaatta ctcatatttt
     6661 tgttgttgtt ggaaatagag caaaactttt tttttcgtcg tgaagagaat aaaattctct
     6721 ttgagacgaa atgcattggt atgtgttatc tttagtagta ttgataatat agtgtgttaa
     6781 acattgcgca ctgcaaaaaa aacatgctgt tcgaattaat agtggttggg gctcgtagaa
     6841 aacgaaaaat atcttaagct agcatagaga atggagcaaa actcaatttg atgcgagctc
     6901 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     6961 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     7021 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     7081 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7141 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7201 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7261 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7321 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7381 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7441 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7501 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     7561 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7621 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7681 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7741 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7801 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7861 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7921 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7981 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8041 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8101 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8161 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8221 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8281 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8341 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8401 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8461 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8521 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8581 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8641 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8701 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8761 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8821 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     8881 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8941 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9001 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9061 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9121 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9181 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9241 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9301 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9361 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9421 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9481 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     9541 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9601 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9661 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     9721 gcaattaacc ctcactaaag ggaacaaaag ct
//
