LOCUS       40924_1564        9669 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector L274-12, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich BV, Samoilovich MP, Satou Y, Kruss M, Gelhaus C, Kurn U, 
            Bosch TC, Khalturin K.
  TITLE     Blood System Formation in the Urochordate Ciona intestinalis 
            Requires the Variable Receptor vCRL1
  JOURNAL   Mol. Biol. Evol. 29 (10), 3081-3093 (2012)
  PUBMED    22513285
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich VB, Samoilovich MP, Satou Y, Kruess M, Gelhaus C, Kuern U,
            Bosch TCG., Khalturin K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2011) Zoological Institute, Christian-Albrechts 
            University Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 
            24118, Germany
REFERENCE   3  (bases 1 to 9669)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9669)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. 
            Evol."; date: "2012"; volume: "29"; issue: "10"; pages: "3081-3093"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2011) Zoological Institute, Christian-Albrechts University 
            Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 24118, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2448..3161
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     regulatory      4119..4128
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4159..4177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4184..4200)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6826..6844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6854..6870)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7012..7467
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7493..7597
                     /label=AmpR promoter
     CDS             7598..8455
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8629..9217
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9505..9526
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9541..9571
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9579..9595
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9603..9619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9640..9658
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccaagt gcttgaaatg ctaaatgttt tcaatttttc gccattaaga caagcctaca
       61 caaatgcttc tataaattat gccaagcacg ttagcagctt ctacgagccc caaccactat
      121 taattcgaac agcatgtttt tttgcagtgc gcaatgttta acacactata ttatcaatac
      181 tactaaagat aacacatacc aatgcatttc gtctcaaaga gaattttatt ctcttcacga
      241 cgaaaaaaaa agttttgctc tatttccaac aacaacaaaa atatgagtaa tttattcaaa
      301 cggtttgctt aagagataag aaaaaagtga ccactattaa ttcgaacgcg gcgtaagctt
      361 accttaatct caagaagagc aaaacaaaag caactaatgt aacggaatca ttatctagtt
      421 atgatctgca aataatgctg cagggaatgt aacttgcttt accatcgcgt ggtgggaaaa
      481 cgacagtcgt tatagcacga gtgttcatac acctcgtgcc agcttacgag ctaccatata
      541 tgttgtgggc gaataaaggt tttataaata taacaggttt tataaatata acaacgccat
      601 tttaaagtcg gttacataat tctgtaacta gttcaaattg aacggtaaac gtaaataaaa
      661 accttgaccg tcttacccaa ttatataaaa acactttgaa cgctttttag gatggaaggg
      721 tatggccatg ctagataatt ctgtggacca tctcaaccca acctattaca gaacggtcgt
      781 aataatgaaa atggatacca tttttaggca tatagactga ttcctctact ttctagaaac
      841 gtaagcagta tacacagaaa aaatgaagtg tgattctgtg caattaaacc gttctaaatt
      901 catagccgac tgaatttcta attaagtgaa tgtctgacct agatttattg ttaagtttag
      961 caccaaatct gagccagcga taagcagtct aattaaattg gctgctggcg ataaaatagg
     1021 tcatcctgaa aaatcgtttg cgcctttatt taaaatatag tagagtgggg aaagacggga
     1081 catcttatcg ttctattttc tcgtcccatt tcgtagtaaa caaagaacat tcaaaaaata
     1141 taaaaccata acttcaaaac ttcaatagac cgttgtcaac tgtttaaaac acagtaagag
     1201 aatttggata ttatgtgcta aaggtgtccc atctcccccc accctactat atctgtttat
     1261 agttctgtgg ggtaagatga gataccgtta acacctaaac atttttactt taaacaatca
     1321 accacgtttt ttatggtcgt gatgggcatg aggttacata attctgaaaa tattttttgc
     1381 ccccgaccaa aagacgcgaa gagtaaaaac atgtctcagc ttatattccc cacataaata
     1441 tatttttgta ctgtttggtg aatttataaa cttatattac catgcatata cgttatgtta
     1501 ctggtatttt ctcagtaggc aaattcattt gtccacgttt tataggtttt tatttatgat
     1561 ttttaaaatg ctaaaaatgt gggagggggt tgaaagtaca atacaaacac acaaaacaac
     1621 tcaaactaaa gatttatagt tatgctaatt cacctacaca atataacaag atgtgtaatg
     1681 caaccatgtg tttatgatga gcgctaacat attttgtaac cactcaaatt ccccgccaca
     1741 cgaggataat gaataggtga ctctgtagtc tgtacatctt agactgaaat aaagattata
     1801 aatctacgaa ataaaataat ttctgctcac tgattatact tctgttttat agattagaaa
     1861 ccgtttctaa taaatgacct aattcgctat acacacacgc tgtgcgcgag ataatcattc
     1921 tcgcaccccg tttattgtgt taaaattgcc gcctagattc acaaagcgtg acggctagag
     1981 ccagcaacgt gtcgccttca attacgcaac atccgggttg cgcaattctg gatataaaag
     2041 aactaacaaa gatgacgtag ctaccttttt cagttcagac ttacgaaaga ctcacgtgtc
     2101 ggcggtctac ttgtcctttt cgagctgtgg caatttggtg agtggttcta tcttatatct
     2161 gagtacatct ctaaggaatt atagtttgat tagttaagtt tttattgtta ggaaagatga
     2221 aatcattagg ttttacttag tttaagtatg ttagtactgg ttaggcgttt gaattattga
     2281 aaaactcagt tcgttaactg tagtagttct ggtagcttag caagtatacc ctgtatacgc
     2341 cttttggctt tttaacaata acttaaactt attttacagc aaatttctgt gcattcggtt
     2401 aaccccaacc ttccaaaatg aaagacaaga ctttaattaa tgaaaaaatg agtaaaggag
     2461 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     2521 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     2581 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttct
     2641 gttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat gactttttca
     2701 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     2761 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     2821 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     2881 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
     2941 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     3001 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     3061 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     3121 cagctgctgg gattacacat ggcatggatg aactatacaa agcgatcgct taccggtcat
     3181 ttataaagat aggcgtgaaa ggtacagtaa aaatcaatat atttatgtgg ggggtttata
     3241 ttaatttaaa tatataacat gctttactat tggtggtgtt tctagtacaa taaaatcatg
     3301 catacatcta cgtggaacaa atacaaaatt agtaataata caattacgtt gtttaataaa
     3361 ccatatgtac ataagaagaa actgagttac acagaatatt gaatacgttt ctcaaacttt
     3421 aattaaaata aaatggttgt cttgaagact caatttatac aaacaatttt aaacacgaac
     3481 aaacagataa aattaaaacc tgggacatgc aaagaatatt caaaacaaaa ttatgaacca
     3541 aagaaactgc taagaatttg aaagtaaaca aatttcttgt ataatgttca aggtacaaca
     3601 ccttcgccat taggaccgtc cggctgtgta ttctttttca agtctggaat ggttatatct
     3661 ggctctggat cttttgttcc ccaagacaag caaagtattg aaaaagcaca acagcaaatc
     3721 tccgtacaaa agataggaat caggaagaat atgatgagtg caacaacaat tatgcatcca
     3781 ataacaatgc ccgctatagc tccatcagat agtgatttac ttctcgaact ttgaatacag
     3841 acagggtcac tgtcaaatgt agcatcctcc tggcatgttg cattaacatc gttcccagta
     3901 tgatcagttg cacatgaata ttgaacttta attatcatgg acaatgtggt aacaacagct
     3961 aaatcacaat ttacggtaac tgtgctgttg cttgtcaatg aagtaagata tgtaagagtg
     4021 gtcataatga ctcctgttag tcctgttcct tcccacgaac atgcagttgg gctagttgat
     4081 actgttgcag tgatcatcag gtttgtggaa tcccgcggcc atggcggccg ggagcatgcg
     4141 acgtcgggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     4201 aacgtcgtga ctgggaaaac cctggcgtta cccgtacggt tcaatattca tgtgcaactg
     4261 atcatactgg gaacgatgtt aatgcaacat gccaggagga tgctacattt gacagtgacc
     4321 ctgtctgtat tcaaagttcg agaagtaaat cactatctga tggagctata gcgggcattg
     4381 ttattggatg cataattgtt gttgcactca tcatattctt cctgattcct atcttttgta
     4441 cggagatttg ctgttgtgct ttttcaatac tttgcttgtc ttggggaaca aaagatccag
     4501 agccagatat aaccattcca gacttgaaaa agaatacaca gccggacggt cctaatggcg
     4561 aaggtgttgt accttgaaca ttatacaaga aatttgttta ctttcaaatt cttagcagtt
     4621 tctttggttc ataattttgt tttgaatatt ctttgcatgt tccaggtttt aattttatct
     4681 gtttgttcgt gtttaaaatt gtttgtataa attgagtctt caagacaacc attttatttt
     4741 aattaaagct tgagaaacgt attcaatatt ctgtgtaact cagtttcttc ttatgtacat
     4801 atggtttatt aaacaacgta attgtattat tactaatttt gtatttgttc cacgtagatg
     4861 tatgcatgat tttattgtac tagaaacacc accaatagta aagcatgtta tatatttaaa
     4921 ttaatataaa ccccccacat aaatatattg atttttactg tacctttcac gcctatcttt
     4981 ataaatggaa ttcttgcgat cgcgtgactg cagccaacga caccgcccac catcacttct
     5041 gcagttttac tccaccagct tgctcaccct tctaaccatt tcagcctagt taaaccccca
     5101 gttcaatttg tcagcctttt aaattgcgtc atgacatttc agttgttact gtgtgccgct
     5161 ctgtccacat ggtgtagtta tgtttttcat tacccccacc gaaaacactt tcgtaggaaa
     5221 aaaccctccg tttcgtgcca acttaaaagt gttatttttg tttctagcgc tgcttaactt
     5281 atttaaaatg acaagactat ctatgatttt aaaccatggc tttgaatggc gtacatgtat
     5341 tattgtgttt atattcagtt aaaccgtact attactgctt ggaaaattca gttcattcct
     5401 tcaccgaagg ctttaacgtg gttggttgcg gtgttgtcaa tttgctgctg gaaataaagc
     5461 ttctgttaag tgtatactgt ctcttggttt gcaagattat tatggatcca gatgttgtat
     5521 atacaagagt gaagatttag cgtttttatg tggtaaaacg cccatggtga tctatttaca
     5581 gtttttaagt tgagctgatc aattttcaca tgatgatcct gaacgaccag gggcacaaag
     5641 gcatttgcag attgtcccat ctctcactac gaccgatcta ccactatttt cacaacggcc
     5701 agtacatttc gaagtctggt atttagcgaa gtagtgttca attgccaaag ccatgtttct
     5761 ctgcttcgct ctgtctggga tgaccgatga tatgggtcgc agctgtaaaa ttacaaatag
     5821 tcagtttttc tgacgaaagt gaatgttgat tacattcaaa tgaaagtcgt tgatggctaa
     5881 tgccatcgtt catacgcttt aattagcaga ttatgtggtt atattagata tctttaccag
     5941 taatattgag aacgccaagg taaaatctag ttttttgata cttaatgaac agccatatta
     6001 aaagccatac atgaaaaaaa aattaaaaaa aacatacata aactctcata agctgacaca
     6061 gtaggtcgtg gctgacacga ggtgtatgaa acaaaacgct cgtgttttaa cgactgttgt
     6121 tgcaccgtca cgcgagaata aagtaacttc cattcataca tgatacagtt cttaccttga
     6181 aatcgatgac tgcagggcta agtttaactg atgctgtcca gttttcgtaa gcatttgttg
     6241 taagtcggtt tctagcaaag aaactgagag cactagctgt atgagactgc ccaccaatca
     6301 cattcgatat aacttcctta gcagccattg taaaacttcc taaatattag taaataacga
     6361 ataataatgt gtacatgact ctgcttatca gttagattgg gcgcgccatc agtttaaacg
     6421 aatgcggccg ccaccgcggg acgttacaca attctaatat taattaaatt attgttttaa
     6481 gtatgatagt aaatcacatt acgccgcgtt cgaattaata gtggtcactt ttttcttatc
     6541 tcttaagcaa accgtttgaa taaattactc atatttttgt tgttgttgga aatagagcaa
     6601 aacttttttt ttcgtcgtga agagaataaa attctctttg agacgaaatg cattggtatg
     6661 tgttatcttt agtagtattg ataatatagt gtgttaaaca ttgcgcactg caaaaaaaac
     6721 atgctgttcg aattaatagt ggttggggct cgtagaaaac gaaaaatatc ttaagctagc
     6781 atagagaatg gagcaaaact caatttgatg cgagctccaa ttcgccctat agtgagtcgt
     6841 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6901 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6961 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     7021 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7081 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7141 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7201 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7261 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7321 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7381 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7441 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7501 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7561 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7621 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7681 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7741 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7801 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7861 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7921 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7981 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8041 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8101 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8161 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8221 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8281 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8341 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8401 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8461 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8521 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8581 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8641 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8701 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8761 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8821 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8881 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8941 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9001 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9061 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9421 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9481 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9541 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9601 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     9661 acaaaagct
//
